Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,280,224 G→T 6.3% G82V (GGT→GTT)  yjcE → putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,280,2240GT 6.3% 329.2 / 10.4 111G82V (GGT→GTT) yjcEputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (44/59);  new base T (5/2);  total (50/61)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

Only 100 of 115 total aligned reads displayed.

TATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGC  >  NC_000913/4280075‑4280370
                                                                                                                                                     |                                                                                                                                                  
tATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATgg                                                                                                                                                    >  1:1527473/1‑150 (MQ=255)
tATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATgg                                                                                                                                                    >  1:1214357/1‑150 (MQ=255)
tATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTGTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATgg                                                                                                                                                    >  1:1311127/1‑150 (MQ=255)
         cGCCATCGGTGCGCTACTGGCGTGGCCGACGGTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGAt                                                                                                                                           <  2:1145611/150‑1 (MQ=255)
          gCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGAtt                                                                                                                                          >  2:658576/1‑150 (MQ=255)
          gCCATCGGTGCGCTACTGGCGAGGCCGACGTTTGGTTTGCATGAGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATATCTCGAACATGGTCGAGAGAtt                                                                                                                                          >  1:1604716/1‑150 (MQ=255)
           ccATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGAttt                                                                                                                                         >  2:728812/1‑150 (MQ=255)
            cATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGAtttt                                                                                                                                        >  2:369301/1‑150 (MQ=255)
            cATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCAGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCGCGAACATGGTCGAGAGAtttt                                                                                                                                        >  1:1707463/1‑150 (MQ=255)
                ggTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGACCATGTTCGAGAAAttcacggg                                                                                                                                    <  2:1102136/150‑7 (MQ=255)
                ggTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGc                                                                                                                                    <  2:518713/150‑1 (MQ=255)
                      cTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTcgcg                                                                                                                              >  2:729742/1‑150 (MQ=255)
                      cTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTcgcg                                                                                                                              >  2:1354585/1‑150 (MQ=255)
                      cTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTcgcg                                                                                                                              >  1:645136/1‑150 (MQ=255)
                       tACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCGTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgc                                                                                                                             <  1:897420/150‑1 (MQ=255)
                       tACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgc                                                                                                                             <  2:362089/150‑1 (MQ=255)
                       tACTGGCGGGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgc                                                                                                                             <  1:141846/150‑1 (MQ=255)
                       tACTGGCGGGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgc                                                                                                                             <  1:1714570/150‑1 (MQ=255)
                        aCTGGCGTGGCCGACGTTTGGTTTGCATGGGGAGTTTGATCCTGATCTCTTTTTAGTCTGGGTTATCCCGCCGTTGCTGTGCGGTGATGGCTGGAAAACGCCGCCCCGTGAATTTCTCGAACTTGGTCGAGAGATTTTCGGCCTCgcgct                                                                                                                            <  1:438049/150‑1 (MQ=255)
                          tGGGGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgctgg                                                                                                                          <  1:759984/150‑1 (MQ=255)
                               tGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgctggcgctg                                                                                                                     >  1:512697/1‑150 (MQ=255)
                               gggCCGACGTTTGGTTTGCATGTGGAGTTTGATCCCGAACTCTTTTTAGGCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCgcgctggcgctg                                                                                                                     <  2:1015493/149‑1 (MQ=255)
                                ggCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGcgctggcgctgg                                                                                                                    >  1:1466428/1‑150 (MQ=255)
                                 gCCGACGGTGGGTTTGCATGTGGAGGTTGATCCTCAACTCTTTTTAGTCTTGGTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCTGCCTCGCGCTGGCGCtggt                                                                                                                   <  1:1519630/150‑1 (MQ=255)
                                  ccGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCAGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCtggtg                                                                                                                  >  1:824964/1‑150 (MQ=255)
                                  ccGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGATGTTCGCTGATGGCTGGAAAACGCCGACACGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCtggtg                                                                                                                  >  1:1234228/1‑150 (MQ=255)
                                   cGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCtggtgg                                                                                                                 >  2:1520662/1‑150 (MQ=255)
                                           ggtttgCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCAcc                                                                                                         <  1:1222545/150‑1 (MQ=255)
                                               tgCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTgg                                                                                                     >  2:1103157/1‑150 (MQ=255)
                                                 cATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGATGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTAGCGCTGGAGCTGGTGGTGGTCACCGTGGTc                                                                                                   >  2:334323/1‑150 (MQ=255)
                                                   tgtgGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCTACCCGTGAATTTCTCGAACATGGTAGAGAGATTTTCGGCCTCGCGCTGGCGCTTGTGGTGGTCACCGTGGTCgg                                                                                                 >  2:1107250/1‑150 (MQ=255)
                                                    gaggAGTTTGATCCTGAACTCTTTTTAGTCTTCTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACACGTGAATAACTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGCTGGTGGTCACCGTGGTCGGc                                                                                                >  1:745382/3‑150 (MQ=255)
                                                       gAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATTGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGCTCACCGTGGTCGGCAtt                                                                                             >  1:865420/1‑150 (MQ=255)
                                                       gAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCAtt                                                                                             <  1:1350977/150‑1 (MQ=255)
                                                        aGTTTGATCCTGAACTCATTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACACGTGAATTTCTCGAACATTGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGCTGGTCACCGTGGTCGGCATTg                                                                                            >  1:213402/1‑150 (MQ=255)
                                                         gTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTTCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTgg                                                                                           >  1:1177556/1‑150 (MQ=255)
                                                         gTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTgg                                                                                           <  1:997554/150‑1 (MQ=255)
                                                         gTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTgg                                                                                           <  2:706123/150‑1 (MQ=255)
                                                           ttGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCt                                                                                         >  1:1443606/1‑150 (MQ=255)
                                                           ttGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCt                                                                                         >  2:53052/1‑150 (MQ=255)
                                                             gATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGAATTTCGGCCTCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGGATTGGCttg                                                                                       >  2:1144005/1‑149 (MQ=255)
                                                              aTCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTTGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTcc                                                                                      <  1:970899/150‑1 (MQ=255)
                                                               tCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCt                                                                                     >  2:1240572/1‑150 (MQ=255)
                                                               tCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCt                                                                                     <  1:1219119/150‑1 (MQ=255)
                                                                    aaCTCTTTTTAGTCTTGTTTAACCCGCCGTTGCTGTTCGCTGATGCCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGGCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTAttt                                                                                <  2:390061/150‑1 (MQ=255)
                                                                    aaCTCTTTTTAGTCTTGTATCTCCCGCCGTCGCAGTGCGCTGATGTCTGGAAAACGACGACCCGTGTATTTCTCGAACTTGGTCGAGAGATTTTCGGCCTCGAGCTGGCGCTGGTGGTGGTCAGCGTGGTCGGCATTGGCTTCCTTAttt                                                                                <  2:56017/150‑1 (MQ=255)
                                                                             tAGTCGTGATTATCCCGCCGTTGATGTTCGCTGAAGGCTGGATCACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGGTTTGGCTTCCTTATTTACTGggtgg                                                                       <  2:1068309/150‑1 (MQ=255)
                                                                              aGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGggtggt                                                                      <  2:540324/150‑1 (MQ=255)
                                                                               gTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGgtggtg                                                                     <  1:729742/150‑1 (MQ=255)
                                                                               gTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGgtggtg                                                                     >  2:518746/1‑150 (MQ=255)
                                                                               gTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGgtggtg                                                                     <  1:1354585/150‑1 (MQ=255)
                                                                                 cTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGcc                                                                   >  1:1162967/1‑150 (MQ=255)
                                                                                    gTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGGCCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCTGCCTCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCggg                                                                >  2:1401806/1‑150 (MQ=255)
                                                                                    gTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCggg                                                                >  1:842347/1‑150 (MQ=255)
                                                                                    gTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCggg                                                                >  1:425496/1‑150 (MQ=255)
                                                                                    gTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGGCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCggg                                                                <  1:1520662/150‑1 (MQ=255)
                                                                                     tttATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGc                                                               <  1:990324/150‑1 (MQ=255)
                                                                                     tttATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGc                                                               <  1:740214/150‑1 (MQ=255)
                                                                                       tATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTGCTGGGTGGTGCCGGGCAt                                                             <  1:1175069/150‑1 (MQ=255)
                                                                                         tCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTc                                                           <  1:177879/150‑1 (MQ=255)
                                                                                          cccGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTcc                                                          <  2:1482044/150‑1 (MQ=255)
                                                                                             gccgTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCt                                                       <  1:2722/150‑1 (MQ=255)
                                                                                             gccgTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCt                                                       >  1:1278103/1‑150 (MQ=255)
                                                                                               cgTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGa                                                     <  1:371131/150‑1 (MQ=255)
                                                                                                 ttGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATc                                                   <  1:971197/150‑1 (MQ=255)
                                                                                                 ttGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATc                                                   <  1:685313/150‑1 (MQ=255)
                                                                                                  tGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGCTCGAGAGATTTTCGGCCTCTCGCTGGCGCTGGTGGAGGTCACCGTGGTGGGGTGTGGCGTCCTTTGTTACTGGGTGGTGCCGGGCATTCCGCTGATcc                                                  >  2:337113/1‑150 (MQ=255)
                                                                                                  ggCTGTTCGCTGATGGCTGGAAAACGCCGCCCCGTGAATTTCTCGAACATGGGCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATcc                                                  <  1:719227/149‑1 (MQ=255)
                                                                                                      gTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCTCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGc                                              >  2:204678/1‑150 (MQ=255)
                                                                                                      gTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGc                                              <  2:1466428/150‑1 (MQ=255)
                                                                                                      gTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGc                                              <  1:1119674/150‑1 (MQ=255)
                                                                                                       ttCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCACTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGcc                                             <  2:1524590/150‑1 (MQ=255)
                                                                                                         cGCTGAGGGCTGGACAACGCCGACCCGTTAATTTCTCGATCATGGGCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCTCCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCtt                                           <  1:122124/150‑1 (MQ=255)
                                                                                                          gCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCttt                                          <  2:1352714/150‑1 (MQ=255)
                                                                                                           cTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTg                                         <  1:1194991/150‑1 (MQ=255)
                                                                                                               tGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCt                                     >  1:1621939/1‑150 (MQ=255)
                                                                                                                  cTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTggc                                  >  1:248651/1‑150 (MQ=255)
                                                                                                                    ggAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTgcgccacagc                                >  2:570640/1‑144 (MQ=255)
                                                                                                                        aaCGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGt                            <  1:835157/150‑1 (MQ=255)
                                                                                                                        aaCGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGt                            <  1:1021389/150‑1 (MQ=255)
                                                                                                                         ccGCCGACCCGTGAATTTCTCGATCATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGGCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTg                           <  2:1604716/149‑1 (MQ=255)
                                                                                                                           gCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCt                         <  2:16820/150‑1 (MQ=255)
                                                                                                                            ccGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCtt                        <  1:960843/150‑1 (MQ=255)
                                                                                                                              gACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGGCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTtc                      <  2:1386573/150‑1 (MQ=255)
                                                                                                                                cccGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGGCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTtctc                    <  2:807605/150‑1 (MQ=255)
                                                                                                                                  cGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccg                  <  2:842347/150‑1 (MQ=255)
                                                                                                                                  cGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccg                  <  2:425496/150‑1 (MQ=255)
                                                                                                                                     gAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGCATAGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccgacc               <  2:1198776/150‑1 (MQ=255)
                                                                                                                                      aaTCTCTCGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccgaccg              >  1:800261/1‑150 (MQ=255)
                                                                                                                                      aaTCTCTCGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccgaccg              >  1:1655514/1‑150 (MQ=255)
                                                                                                                                       atctctcGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTccgaccga             >  1:721465/4‑150 (MQ=255)
                                                                                                                                          tctcGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCtt                        <  2:1141524/136‑1 (MQ=255)
                                                                                                                                          tctcGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCtt                        >  1:1141524/1‑136 (MQ=255)
                                                                                                                                           ctcGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCt         >  2:515055/1‑150 (MQ=255)
                                                                                                                                            tcGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCtg        >  1:1722783/1‑150 (MQ=255)
                                                                                                                                             cGACCATGTTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCtgt       >  1:1500834/1‑150 (MQ=255)
                                                                                                                                                aCATGGTCGAGAGATTTTCGGCCTCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTAGTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGgc    <  1:695869/150‑1 (MQ=255)
                                                                                                                                                  ctGGTCGAGAGATTTTCGGCCGCGCGCGGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCAGTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGgcgc  <  2:1214357/149‑1 (MQ=255)
                                                                                                                                                  aTGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGgcgc  <  1:369301/150‑1 (MQ=255)
                                                                                                                                                  aTGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGgcgc  <  1:1384229/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TATGCAAATCGCCATCGGTGCGCTACTGGCGTGGCCGACGTTTGGTTTGCATGTGGAGTTTGATCCTGAACTCTTTTTAGTCTTGTTTATCCCGCCGTTGCTGTTCGCTGATGGCTGGAAAACGCCGACCCGTGAATTTCTCGAACATGGTCGAGAGATTTTCGGCCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGC  >  NC_000913/4280075‑4280370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: