Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,281,679 T→G 6.9% intergenic (+50/+104) yjcE → / ← yjcF putative cation/proton antiporter/pentapeptide repeats protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,281,6790TG 6.9% 472.6 / 20.5 160intergenic (+50/+104)yjcE/yjcFputative cation/proton antiporter/pentapeptide repeats protein
Reads supporting (aligned to +/- strand):  ref base T (78/70);  new base G (6/5);  total (84/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01

Only 100 of 168 total aligned reads displayed.

CCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGAC  >  NC_000913/4281530‑4281827
                                                                                                                                                     |                                                                                                                                                    
 caccTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCtt                                                                                                                                                     >  2:1456616/1‑150 (MQ=255)
   ccTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCtttt                                                                                                                                                   >  2:392576/1‑150 (MQ=255)
   ccTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCtttt                                                                                                                                                   >  1:1546881/1‑150 (MQ=255)
   ccTGAGCGCCACACGTGAGATCAGCAACGAAAGGCTTCAAAAATTACTGCACGATCTCGATTTGATTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTTACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCtttt                                                                                                                                                   <  2:1469393/150‑1 (MQ=255)
    cTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTg                                                                                                                                                  >  1:942570/1‑150 (MQ=255)
    cTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTg                                                                                                                                                  >  2:1272848/1‑150 (MQ=255)
         cgcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCt                                                                                                                                             <  1:1193591/150‑1 (MQ=255)
         cgcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCt                                                                                                                                             >  1:1640653/1‑150 (MQ=255)
         cgcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGCTAACCTGTTAAAGGTGCAAAAGATTGCACCt                                                                                                                                             <  2:376623/150‑1 (MQ=38)
          gcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCtt                                                                                                                                            >  2:648409/1‑150 (MQ=255)
          gcCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAAAAGATTGCACCtt                                                                                                                                            <  1:1266463/150‑1 (MQ=255)
              cacTGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAAc                                                                                                                                        <  1:1721678/150‑1 (MQ=255)
              cacGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAAc                                                                                                                                        >  1:1063702/1‑150 (MQ=255)
              cacGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAAc                                                                                                                                        >  2:1552817/1‑150 (MQ=255)
               acGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATCGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACa                                                                                                                                       >  1:200273/1‑150 (MQ=255)
                  ggAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGt                                                                                                                                    <  1:67481/150‑1 (MQ=255)
                   gagaTCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCATTTGCACCTTTAACAGGtt                                                                                                                                   <  2:694853/150‑1 (MQ=255)
                    agaTCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTa                                                                                                                                  <  2:243788/150‑1 (MQ=255)
                      aacAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGAAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGc                                                                                                                                >  1:1152385/3‑150 (MQ=255)
                        cAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTc                                                                                                                              >  2:184850/1‑150 (MQ=255)
                         aGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCg                                                                                                                             >  2:1513847/1‑150 (MQ=255)
                         aGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCg                                                                                                                             >  2:832611/1‑150 (MQ=255)
                           cAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCggg                                                                                                                           >  2:604513/1‑150 (MQ=255)
                             aCGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCt                                                                                                                         >  2:660766/1‑150 (MQ=255)
                             aCGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTAGCACCTTTAACAGGTTAGCTCGGGCt                                                                                                                         >  1:1113375/1‑150 (MQ=255)
                                   cGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACAtt                                                                                                                   >  1:774621/1‑150 (MQ=255)
                                     cTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCa                                                                                                                 <  1:415162/150‑1 (MQ=255)
                                           aaaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTc                                                                                                           <  2:315811/150‑1 (MQ=255)
                                            aaTTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCt                                                                                                          <  2:893899/150‑1 (MQ=255)
                                              ttACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTaa                                                                                                        <  1:979035/150‑1 (MQ=255)
                                                 cTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAAc                                                                                                     >  2:1020217/1‑150 (MQ=255)
                                                 cTGCACGATCTAGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGATCGTGCTAACATGCATGAGTCTAAAAc                                                                                                     >  2:1013920/1‑150 (MQ=255)
                                                  tGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACa                                                                                                    >  1:492705/1‑150 (MQ=255)
                                                   gCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaa                                                                                                   >  2:769370/1‑150 (MQ=255)
                                                    cACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACaaa                                                                                                  <  1:637934/150‑1 (MQ=255)
                                                          ctcGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTACGCCGAAAGCGACGTAACCAGGCCCGAGTTAATCTGAAAAATGTGCAATCTTTTGCACGTTTAACAGGTTAGCTCGGGCTTACATTCATGATTCTAAAACAAATAAAtt                                                                                            <  1:6799/150‑1 (MQ=255)
                                                          ctcGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAAtt                                                                                            <  2:1337096/150‑1 (MQ=255)
                                                              aTTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTAtct                                                                                        >  1:1655070/1‑150 (MQ=255)
                                                                 tGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTAtctctc                                                                                     <  2:579300/150‑1 (MQ=255)
                                                                   cTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCtt                                                                                   >  2:354984/1‑150 (MQ=255)
                                                                    ttGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCttt                                                                                  <  1:912948/150‑1 (MQ=255)
                                                                     ttaaGCGTTGCTAATTTAGGAAAAACAGTAAGCCTAAAGCTACGTAACCAGGCCCGAGTTAATATGATAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCtttt                                                                                 <  1:855572/148‑1 (MQ=255)
                                                                       aaGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTaa                                                                               <  2:1700389/150‑1 (MQ=255)
                                                                         gCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAg                                                                             <  1:1413153/150‑1 (MQ=255)
                                                                           gTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTc                                                                           <  1:1703652/150‑1 (MQ=255)
                                                                           gTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTc                                                                           >  1:893191/1‑150 (MQ=255)
                                                                               cTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCATTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAAtt                                                                       >  2:611456/1‑150 (MQ=255)
                                                                                tAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTc                                                                      <  2:1593217/150‑1 (MQ=255)
                                                                                   ttGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCAtt                                                                   <  2:942570/150‑1 (MQ=255)
                                                                                     gAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTgg                                                                 <  2:1261547/150‑1 (MQ=255)
                                                                                       ggAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTTCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCt                                                               >  1:1452142/1‑150 (MQ=255)
                                                                                       ggAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCt                                                               >  2:1323555/1‑150 (MQ=255)
                                                                                       ggAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCt                                                               >  1:581813/1‑150 (MQ=255)
                                                                                           aaTCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGttc                                                           <  1:1323555/150‑1 (MQ=255)
                                                                                              cactaccccGAACGCGACGTACCCAGGCCCGAGGTAATCTGAAAAAGCTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGttcttc                                                        <  2:1654712/143‑1 (MQ=255)
                                                                                                gTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGa                                                      >  1:1107817/1‑150 (MQ=255)
                                                                                                 tAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGAc                                                     <  2:760698/150‑1 (MQ=255)
                                                                                                  aaGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACt                                                    <  1:765663/150‑1 (MQ=255)
                                                                                                  aaGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCAACTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACt                                                    <  1:1135871/150‑1 (MQ=255)
                                                                                                    gCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACttt                                                  >  2:1512489/1‑149 (MQ=255)
                                                                                                     ccGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAAGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTaa                                                 >  1:1206218/1‑150 (MQ=255)
                                                                                                      cGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAAc                                                <  2:1640653/150‑1 (MQ=255)
                                                                                                       gAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAAcc                                               <  1:1355632/150‑1 (MQ=255)
                                                                                                       gAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAAcc                                               <  2:1063702/150‑1 (MQ=255)
                                                                                                           gCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCNAACANTCATGATTNTAAAACAANTAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCaa                                           <  1:1456616/150‑1 (MQ=255)
                                                                                                             gACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATc                                         <  1:1272848/150‑1 (MQ=255)
                                                                                                             gACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATc                                         <  2:502418/150‑1 (MQ=255)
                                                                                                               cGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTg                                       >  2:458592/1‑150 (MQ=255)
                                                                                                               cGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTg                                       <  1:1349975/150‑1 (MQ=255)
                                                                                                                gTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGc                                      >  1:1024670/1‑150 (MQ=255)
                                                                                                                 tAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCa                                     <  1:832611/150‑1 (MQ=255)
                                                                                                                 tAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCa                                     <  2:1175639/150‑1 (MQ=255)
                                                                                                                   aCCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATc                                   <  2:1429227/150‑1 (MQ=255)
                                                                                                                    ccAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTATCTTTTAAAGTCAATTCATTGGCTGTTCTTCGAATTAACCTCAATCTGCAtat                                  >  2:852924/1‑148 (MQ=255)
                                                                                                                     cAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCtt                                 >  1:660209/1‑150 (MQ=255)
                                                                                                                       ggCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGc                               >  2:844368/1‑150 (MQ=255)
                                                                                                                         cccGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCat                             >  1:251611/1‑150 (MQ=255)
                                                                                                                            gAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTATTCGACTTAACCTCAATCTTCATCTTGCataat                          >  2:870195/1‑150 (MQ=255)
                                                                                                                                tAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCt                      <  2:568795/150‑1 (MQ=255)
                                                                                                                                 aaTCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTg                     >  2:1184968/1‑150 (MQ=255)
                                                                                                                                  aTCTGAAAAAGGTGCTATCTTTTGCAACTTTAACTGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAAATATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGc                    <  1:270710/150‑1 (MQ=255)
                                                                                                                                  aTCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGc                    >  2:911551/1‑150 (MQ=255)
                                                                                                                                  aTCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGc                    <  2:1572631/150‑1 (MQ=255)
                                                                                                                                  aTCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGc                    <  1:1573091/150‑1 (MQ=255)
                                                                                                                                    atGAAAAAGGTGCAATCTTTTTCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAg                  <  1:627411/149‑1 (MQ=255)
                                                                                                                                      gttaaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAAAAAATAAATTATCTATCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTa                >  1:702294/4‑150 (MQ=255)
                                                                                                                                      gAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTa                <  2:1546881/150‑1 (MQ=255)
                                                                                                                                      gAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTa                >  1:494283/1‑150 (MQ=255)
                                                                                                                                        taaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGCCTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAAt              >  1:1627043/2‑150 (MQ=255)
                                                                                                                                        aaaaGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAAt              >  1:1122692/1‑150 (MQ=255)
                                                                                                                                         aaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATc             >  1:1693338/1‑150 (MQ=255)
                                                                                                                                         aaaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATc             >  2:1170513/1‑150 (MQ=255)
                                                                                                                                          aaGGTGCAAAAGATTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCt            >  1:843707/1‑150 (MQ=255)
                                                                                                                                           aGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCtt           >  2:986183/1‑150 (MQ=255)
                                                                                                                                            ggTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTc          <  2:1392650/150‑1 (MQ=255)
                                                                                                                                             gTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCa         >  1:409707/1‑150 (MQ=255)
                                                                                                                                              tGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCAt        <  2:774621/150‑1 (MQ=255)
                                                                                                                                              tGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCAt        <  1:458592/150‑1 (MQ=255)
                                                                                                                                               gCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATa       >  1:793630/1‑150 (MQ=255)
                                                                                                                                                    cTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGAc  <  2:1494859/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAAGCGTTGCTAATTGAGGAAAATCAGTAAGCCGAAAGCGACGTAACCAGGCCCGAGGTAATCTGAAAAAGGTGCAATCTTTTGCACCTTTAACAGGTTAGCTCGGGCTAACATTCATGATTCTAAAACAAATAAATTATCTCTCTTTTAAAGTCAATTCATTGGCTGTTCTTCGACTTAACCTCAATCTGCATCTTGCATAATATCTGCAGTAATCTTCATAGCGAC  >  NC_000913/4281530‑4281827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: