Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,283,230 A→G 5.4% intergenic (‑155/+23) yjcF ← / ← actP pentapeptide repeats protein/acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,283,2300AG 5.4% 326.1 / 5.7 113intergenic (‑155/+23)yjcF/actPpentapeptide repeats protein/acetate transporter
Reads supporting (aligned to +/- strand):  ref base A (47/58);  new base G (2/4);  total (49/64)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

Only 100 of 114 total aligned reads displayed.

TCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGG  >  NC_000913/4283082‑4283379
                                                                                                                                                    |                                                                                                                                                     
tCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAg                                                                                                                                                      <  2:1373197/150‑1 (MQ=255)
  cTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAgac                                                                                                                                                    >  2:933973/1‑150 (MQ=255)
  cTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAgac                                                                                                                                                    >  2:67007/1‑150 (MQ=255)
       aaTTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCg                                                                                                                                               >  2:110069/1‑150 (MQ=255)
          aaaCAAGAAACAAACGGCATATTCTCTGAAATATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGGCAGACGACCgggg                                                                                                                                            <  1:1675902/149‑1 (MQ=255)
           aaCCAGAAAGAAAAGGCAAATTCTCTGCATTATCATTTTCTACGACTGTCAAAAAGCCCTCATTTTTTAATGAGTTTATTTTTTTAATATTATGGGAACAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCGTCTGACCGGGGa                                                                                                                                           <  2:868473/150‑1 (MQ=255)
           aaCAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGTAGAGGAAGAGTATTCCCCGCTCAAACCACCGGGGa                                                                                                                                           >  2:813212/1‑150 (MQ=255)
            aCAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCGTCTGACCGGGGaa                                                                                                                                          <  1:252981/150‑1 (MQ=255)
             cAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCGTCTGACCGGGGaat                                                                                                                                         <  1:422927/150‑2 (MQ=255)
             aaagaaagaAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAg                                                                                                                                         <  2:1389861/149‑1 (MQ=255)
              aataaataaaAGGAATATTCTCTTCATTATCTTTTTCTACGACTGTCAAAAATCGCTAATTTTTAAATGTTTTTTTTTGTTTAATATTATGGTAAACGGTTATGCATTTGGGAGAGGAAGAGTATTCCCCGGGCAGACGACCGGGGAAgg                                                                                                                                        <  2:1417605/147‑1 (MQ=255)
              aagaaagaAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAgg                                                                                                                                        >  1:992887/1‑150 (MQ=255)
              aagaaagaAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAg                                                                                                                                         >  2:1706032/1‑149 (MQ=255)
               agaaagaaAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCGTCTGACCGGGGaatac                                                                                                                                       <  2:507333/150‑4 (MQ=255)
               agaaagaaAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTAGGAGAGTAAGAGTATTACCCTGTCAGACGAACGGGGAAggg                                                                                                                                       >  1:200230/1‑150 (MQ=255)
                gaaagaaaCGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGGGATGCATGTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAgggg                                                                                                                                      <  1:56422/150‑1 (MQ=255)
                   agaaaAGGCATATTCTCTGTATTATCTTTTTCTACGACTGTCAAAAATCGCTCATTTTTTATTTATTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGgaga                                                                                                                                   <  2:543700/150‑1 (MQ=255)
                    caaaaGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGgagag                                                                                                                                  <  1:419990/149‑1 (MQ=255)
                     aaaaGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGATTATTTGTTTAATATTATGGCAAAAGGTGCTGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGgagaga                                                                                                                                 <  1:456456/150‑1 (MQ=255)
                       aaGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGAtt                                                                                                                               <  1:433482/150‑1 (MQ=255)
                       aaGGCATATTCTCTCGATAATCATTTTCTACCCCTCTCAAAAATCGCACATTTTTTAATGCTTTTATATGTTTAATATTATGGCACACGGTGCTGCATTTGGTAGAGGAAGAGTATTCCCCGGTCAGACTACCGGGGAAGGGGAGAGAtt                                                                                                                               <  2:872111/150‑1 (MQ=255)
                       aaGGAATATTCTCTGCATTATCATTTTATACGACTGTCAAAAATCGCTAATTTTTTAATGATTTTATTTTTTTAATATTATGGGAAAAGGTGATGCATTTGGGTGAGGAAGAGTATTCCCCGGTCTGACGACCGGGGAAGGGGAGAGAtt                                                                                                                               <  2:504667/150‑1 (MQ=255)
                          gCATATTCTCTGAATTATCATTTTCTACGACTGTAAAAAATCGCTCATTATTTAAAGATTTTATTTTTTTAATATTATGGTAAAAGGTGATGCATTTGGGACACTAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAAt                                                                                                                            <  2:1462784/150‑1 (MQ=255)
                           cATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATg                                                                                                                           <  1:696980/150‑1 (MQ=255)
                           cATATTCTCTGCATTATCATTTTCTACGACCGTCAAAAATCGCTCATTTTTTAATGAGTTTATATGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATg                                                                                                                           <  1:105957/150‑1 (MQ=255)
                           cATATTCTCTGCATTATCAATTTCTACGACTGTCAAAAATCGCTCATTATTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATg                                                                                                                           <  1:836647/150‑1 (MQ=255)
                             tatTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGTGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATgcg                                                                                                                         >  1:371984/1‑150 (MQ=255)
                              atTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATgcgc                                                                                                                        >  2:669492/1‑150 (MQ=255)
                               ttCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGGCAGACGACCGGGGAAGGGGAGAGATTAATgcgcg                                                                                                                       <  2:1290265/150‑1 (MQ=255)
                                   ctGCTTTATCATTATCTACGACTGACAAAAATCGCTAATTTTTTAATGAGTTTATTTTTTAAATATTATGGCAAAAGGTCATGCATTTGGTAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGc                                                                                                                   <  1:575564/150‑1 (MQ=255)
                                    tGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATTCGCGCGGcc                                                                                                                  >  2:327011/1‑150 (MQ=255)
                                     gCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGTGAGAGATTAATGAGCGCggaat                                                                                                                 >  1:974547/1‑147 (MQ=255)
                                      cATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATTCGCGCGGccat                                                                                                                >  2:608483/1‑148 (MQ=255)
                                          aTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGATTATTCCCCGGTCAGACGAACGGGGAATGGGATAGATTAATTCACGAGGCCTTgata                                                                                                            >  1:837538/1‑147 (MQ=255)
                                          aTCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTc                                                                                                            >  2:625441/1‑150 (MQ=255)
                                           tCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAATGGGAGTGATTAATGCGCGCGGCCTTGCTCa                                                                                                           >  1:1146941/1‑150 (MQ=255)
                                            cATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGAGCGCGGCCTTGCTCaa                                                                                                          >  1:544863/1‑150 (MQ=255)
                                              ttttCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGACGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACg                                                                                                        <  1:9469/150‑1 (MQ=255)
                                                    aCGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCATGCTCAACGCCAAAg                                                                                                  >  1:86900/1‑150 (MQ=255)
                                                     cGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGc                                                                                                 >  2:1457667/1‑150 (MQ=255)
                                                       aCTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCg                                                                                               <  1:287481/150‑1 (MQ=255)
                                                       aCTGGCAAAAATAGCTCATTTTTTTATGAGTTTATATGTTTAATATTATGGGAAAAGGTCATGCATTTGGGAGAGGACGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCg                                                                                               <  1:262731/150‑1 (MQ=255)
                                                          gTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAATGGGAGAGATTAATGAGCGCGGACTTGCTCAACGCCAAATCCGGTc                                                                                            >  1:1070031/1‑150 (MQ=255)
                                                          gTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGAAGACCGGGGAAGGGGAGAGATTAATGCGCGCGGACTTGCTAAACGCCAAATCCGGTc                                                                                            >  1:1190210/1‑150 (MQ=255)
                                                          gTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTAAGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAATGGGAGATATTAATTCGCGCGGCCTTTATAAACCCCAAAGCCGGta                                                                                            >  2:1442238/1‑149 (MQ=255)
                                                            cAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTg                                                                                          >  2:1611990/1‑150 (MQ=255)
                                                            cAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTg                                                                                          >  2:66736/1‑150 (MQ=255)
                                                              aaaaTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTggg                                                                                        >  1:438327/1‑150 (MQ=255)
                                                              aaaaTCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTggg                                                                                        <  2:1454052/150‑1 (MQ=255)
                                                              aaaaTCGCTCATTTTTTAATGAGTTAATATGTTAAAAATAATGGGAAAAGGTTATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTggg                                                                                        <  1:443676/150‑1 (MQ=255)
                                                                  tCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                    <  1:487978/150‑1 (MQ=255)
                                                                  aaacTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCg                                                                                    >  2:1712195/4‑150 (MQ=255)
                                                                     cTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGGGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGAt                                                                                 <  1:1618355/150‑1 (MQ=255)
                                                                       cATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGAACGGGGTATGGGAGAGATTAATGCGCGCGGCCTTTCTCAACGCCAAATCCGGTCTGGGAGCGGATaa                                                                               >  1:866235/1‑150 (MQ=255)
                                                                       cATTTTTTAATGAGTTTATTTGTTTAATATTATGCGAAACGGCGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATaa                                                                               <  1:848934/150‑1 (MQ=255)
                                                                        aTTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATaaa                                                                              >  1:1654009/1‑150 (MQ=255)
                                                                        aTTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATaaa                                                                              >  1:692731/1‑150 (MQ=255)
                                                                          tttttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACt                                                                            <  2:964507/150‑1 (MQ=255)
                                                                          tttttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACt                                                                            <  1:1351432/150‑1 (MQ=255)
                                                                            tttAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCCGTCAGACGACCGGCGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTgc                                                                          >  2:663201/1‑150 (MQ=255)
                                                                                aTGAGTTTATTTGTTTAATATTATGGGACACGGGGATGCCTTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTgcgcgc                                                                      <  1:784610/150‑1 (MQ=255)
                                                                                   aGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGa                                                                   >  2:1389185/1‑150 (MQ=255)
                                                                                          ttGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtc                                                            <  1:933973/150‑1 (MQ=255)
                                                                                            gTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCACACGACCGGGGAAGGGGAGAGATTTATGCGCCCGGCCTTGCAAAACGCCAAAGCCGGTCTGGGAGCGGATAAACAGCGCGCGGAACAGTtcac                                                          >  1:1268415/1‑150 (MQ=255)
                                                                                             tttAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacg                                                         <  1:1632963/150‑1 (MQ=255)
                                                                                             tttAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCCGCCCTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacg                                                         <  2:276692/150‑1 (MQ=255)
                                                                                                 atatTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTtcacgctca                                                     <  1:1170223/150‑1 (MQ=255)
                                                                                                   attatGGGAAAAGGTGATTCATTTGGGAGAGGAAGAGTATTCACCGGTAAGACGAACGGGGAACCGGACAAATTAATTCGCGCGGCCTTGCTAAACGCCCAAACCGCTCTTGGAGCGGATAAACAGCGCGCGGAACATTtaaccctaaac                                                   >  2:1259677/1‑146 (MQ=255)
                                                                                                     tatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACAGCGCGCGGAACAGTTCACGCTCAcgcg                                                 >  1:613857/1‑150 (MQ=255)
                                                                                                     tatGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCCGTGAGACGACCGGGGAAGGGGAGAGAATAATTCGCGCGGCCTTGCTCAACGCCAAAGCCGGGCTGGGAGCGGATAAACAGCCCGCGGCACAGTtaacgcacacgcc                                                 >  2:1531527/1‑149 (MQ=255)
                                                                                                       tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCAcgcgcg                                               <  1:1389185/150‑1 (MQ=255)
                                                                                                       tGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCAcgcgcg                                               <  1:1673393/150‑1 (MQ=255)
                                                                                                        gggAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATTCGCGCGGCCTTGCTCAACCCAAAAGCAGGTCATGGAGCGGATAAAATTAGCGCGGAACAGTTAAACCTAAcgcgccc                                              >  2:1285704/1‑148 (MQ=255)
                                                                                                         ggAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGTAGGGGAGAGAATAATGCGCGCGGCCATGCTCAACGCCAAAGCCGGGCTGGGCGCGGATTAACTGCGCGCGGACCCGTTCCCGCTCCCGCGCGcc                                             >  1:615563/1‑150 (MQ=255)
                                                                                                         ggAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGcc                                             <  2:353598/150‑1 (MQ=255)
                                                                                                          gAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCt                                            >  1:1125855/1‑150 (MQ=255)
                                                                                                          gAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCCGGGAAGGGGAGAGATTAATGCGCGCGGCCTTTCTAAACGCCCAAGCCGGTCTGGGAGCGGATAAAATGCGCGCGGAACAGTTCACGCTCACGCGCGccg                                            >  1:541333/1‑149 (MQ=255)
                                                                                                            caatgTGATGCATTTGGGAGAGTAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAACGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTc                                          <  1:169571/146‑1 (MQ=255)
                                                                                                                 tGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTg                                     >  1:1137403/1‑150 (MQ=255)
                                                                                                                    tGCATTTGGGAGAGGAAGAGTATACCCCGGTCTGACGACCGGGGAAGGGGAGAGATAAATGCGCGCGGCCTTGCTCAACGCCATAGCCGGTCTGGGAGCGGATAAACTGCGCGCGTAACAGTTCACGCTCACGCGCGCCTTCAGCGGAGt                                  <  1:1567661/150‑1 (MQ=255)
                                                                                                                        tttGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATc                              <  1:327011/150‑1 (MQ=255)
                                                                                                                          ggggAGAGGCAGACGATTCCCCGGTCAGCCGACCGGGGAAGGGGAGAGCTTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGGCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCgg                            <  1:305889/149‑1 (MQ=255)
                                                                                                                           gggAGAGGAAGAGTATGCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGt                           <  1:683677/150‑1 (MQ=255)
                                                                                                                             gagaGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAGCGCCAAAGCCGGTCTGGGAGCGGATTAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCGTCCGCGGAGTTATCGGGTg                         >  1:1137375/1‑150 (MQ=255)
                                                                                                                                agGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCTCGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCg                      <  2:837538/150‑1 (MQ=255)
                                                                                                                                agGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCg                      <  2:583206/150‑1 (MQ=255)
                                                                                                                                  gAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCgag                    <  2:438327/150‑1 (MQ=255)
                                                                                                                                     gagTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGagaag                 <  1:1611990/150‑1 (MQ=255)
                                                                                                                                        tATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAc              <  1:1457667/150‑1 (MQ=255)
                                                                                                                                        tATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAc              <  1:669492/150‑1 (MQ=255)
                                                                                                                                        ccttCCCCGGTCGTCTGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCCCGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAc              >  1:517500/3‑150 (MQ=255)
                                                                                                                                         aTTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAcc             <  1:1442238/150‑1 (MQ=255)
                                                                                                                                         aTTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAcc             <  1:511566/150‑1 (MQ=255)
                                                                                                                                         aTTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAAcc             <  2:1204039/150‑1 (MQ=255)
                                                                                                                                           tCCCCGGTCGTCTGACCGGGGAAGGGCAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGCATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGATATCGGTTGCCGAGAAGAACCAg           >  2:907960/1‑150 (MQ=255)
                                                                                                                                              ccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATg        <  1:833292/150‑1 (MQ=255)
                                                                                                                                              ccGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCGTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATg        <  1:1259677/150‑1 (MQ=255)
                                                                                                                                                ggTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGcc      <  1:401028/150‑1 (MQ=255)
                                                                                                                                                   cAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAg   >  2:1605966/1‑150 (MQ=255)
                                                                                                                                                    aGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAgg  >  2:696132/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAGGAAGAGTATTCCCCGGTCAGACGACCGGGGAAGGGGAGAGATTAATGCGCGCGGCCTTGCTCAACGCCAAAGCCGGTCTGGGAGCGGATAAACTGCGCGCGGAACAGTTCACGCTCACGCGCGCCTTCCGCTGAGTTATCGGTTGCCGAGAAGAACCAGATGCCGAGG  >  NC_000913/4283082‑4283379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: