Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,299,543 A→G 8.0% intergenic (‑177/+21) fdhF ← / ← mdtP formate dehydrogenase‑H, selenopolypeptide subunit/outer membrane factor of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,299,5430AG 8.0% 318.8 / 14.5 112intergenic (‑177/+21)fdhF/mdtPformate dehydrogenase‑H, selenopolypeptide subunit/outer membrane factor of efflux pump
Reads supporting (aligned to +/- strand):  ref base A (56/47);  new base G (3/6);  total (59/53)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

Only 100 of 112 total aligned reads displayed.

CATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGC  >  NC_000913/4299395‑4299687
                                                                                                                                                    |                                                                                                                                                
cATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAg                                                                                                                                                 <  1:1559710/150‑1 (MQ=255)
cATACGCGCTCTCGACTACAGTATGCATCTTTTATCCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTCACGTGACGAAACGAAATACCGCGTCACAg                                                                                                                                                 >  1:782662/1‑150 (MQ=255)
    cgcgCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCAt                                                                                                                                             >  1:209695/1‑150 (MQ=255)
    cgcgCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCAt                                                                                                                                             >  2:4861/1‑150 (MQ=255)
    cgcgCTCTCGACTACAGTATGCATCTTTTATGCCACATTTAATGTGGGGTCATTCCCTGATATTACGGGCACTAATTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCACTATTGACGTGACGAAACGCAATACCGCGTGACAGccct                                                                                                                                             >  2:240630/1‑148 (MQ=255)
      cgcTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCa                                                                                                                                           >  2:1460899/1‑150 (MQ=255)
        ctctCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAcg                                                                                                                                         >  1:1456260/1‑150 (MQ=255)
          ctcGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAcgcg                                                                                                                                       >  1:243597/1‑150 (MQ=255)
           tcGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGATGGCTGTCACGCgg                                                                                                                                      <  1:430472/150‑1 (MQ=255)
            cGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGATGGCTGTCACGCggt                                                                                                                                     <  2:920438/150‑2 (MQ=255)
            cGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCCCGCGGc                                                                                                                                     >  1:684457/1‑150 (MQ=255)
            cGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCAATCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGGCGGCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGc                                                                                                                                     <  1:1544036/150‑1 (MQ=255)
             gACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCAGAAATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGATGGCTGTCACGCggta                                                                                                                                    <  1:494220/150‑3 (MQ=255)
              aCTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTACCTGATATTACGGGCACTATTTATTCAAAACTCTGAAGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGATGGCTGTCACGCggtat                                                                                                                                   <  2:275861/150‑4 (MQ=255)
                 aCAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGATGGCTGTCACGCggtatttc                                                                                                                                <  1:1122811/150‑7 (MQ=255)
                 aCAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACa                                                                                                                                >  2:718530/1‑150 (MQ=255)
                 aCAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACa                                                                                                                                >  1:1307176/1‑150 (MQ=255)
                  cAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAAAAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCCCGCGGCAGACat                                                                                                                               >  2:1073733/1‑150 (MQ=255)
                   aGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGGCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACatt                                                                                                                              <  1:1044224/150‑1 (MQ=255)
                        gCATCTTTTATGCCACATTTTATGTTGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACattttatt                                                                                                                         <  2:35315/150‑1 (MQ=255)
                        gCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACattttatt                                                                                                                         <  2:29618/150‑1 (MQ=255)
                         cATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACattttattt                                                                                                                        >  2:643653/1‑150 (MQ=255)
                         cATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTAATCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACattttattt                                                                                                                        >  2:1279047/1‑150 (MQ=255)
                               ttATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTtctc                                                                                                                  <  2:94297/150‑1 (MQ=255)
                                tATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcg                                                                                                                 >  1:1470847/1‑150 (MQ=255)
                                   gCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcgacg                                                                                                              >  2:199634/1‑150 (MQ=255)
                                    ccACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcgacga                                                                                                             >  2:243690/1‑150 (MQ=255)
                                       caTTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACgg                                                                                                          <  1:838566/150‑1 (MQ=255)
                                          tttATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAc                                                                                                       <  1:1540074/150‑1 (MQ=255)
                                          tttATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAc                                                                                                       <  1:132539/150‑1 (MQ=255)
                                           ttATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcc                                                                                                      >  2:1699805/1‑150 (MQ=255)
                                           ttATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcc                                                                                                      <  1:347137/150‑1 (MQ=255)
                                             aTGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcctg                                                                                                    <  2:375931/150‑1 (MQ=255)
                                               gtgGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcctgcc                                                                                                  <  1:891464/150‑1 (MQ=255)
                                                 ggggTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcctgcctg                                                                                                <  2:1276315/150‑1 (MQ=255)
                                                    gTCATTCCCTGATATTACTGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATa                                                                                             <  1:595479/150‑1 (MQ=255)
                                                      cATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATAcc                                                                                           >  1:799056/1‑150 (MQ=255)
                                                      cATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATAcc                                                                                           >  2:1264709/1‑150 (MQ=255)
                                                      cATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATAcc                                                                                           >  2:710285/1‑150 (MQ=255)
                                                       aTTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATAccc                                                                                          >  1:401246/1‑150 (MQ=255)
                                                       aTTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATAccc                                                                                          >  2:714834/1‑150 (MQ=255)
                                                       aTTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACAAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATGATTTCTCGACTACGGGCCCAGCCTGATAccc                                                                                          >  1:125482/1‑150 (MQ=255)
                                                        ttCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACccg                                                                                         >  2:1180638/1‑150 (MQ=255)
                                                         tCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATAACGCGGCAGACATTTTATTTTTTCTCGACGACGTGACCTGCCTGATACccgc                                                                                        >  2:1667171/1‑150 (MQ=255)
                                                          cccTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACccgcc                                                                                       >  2:177276/1‑150 (MQ=255)
                                                          cccTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACccgcc                                                                                       <  2:243597/150‑1 (MQ=255)
                                                          cccTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACccgcc                                                                                       <  2:708167/150‑1 (MQ=255)
                                                            cTGATATTACGGGCACTATTTATACAAAACTCTGTCGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCGCGCGTGCTCCATTTTAGTTTTTCTCGACGACGGGACATGCCTGCTACCcgcagc                                                                                     >  2:266058/1‑150 (MQ=255)
                                                                tatTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATAACGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAg                                                                                 >  1:110438/1‑150 (MQ=255)
                                                                 atTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGc                                                                                >  1:176492/1‑150 (MQ=255)
                                                                     cgtgCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCCTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGAtt                                                                            <  1:1026636/147‑1 (MQ=255)
                                                                      gggCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGAttt                                                                           >  2:1004925/1‑150 (MQ=255)
                                                                       ggCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTtc                                                                          >  1:543791/1‑150 (MQ=255)
                                                                        gCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGGCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTtca                                                                         <  1:1590007/150‑1 (MQ=255)
                                                                         cACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTtcat                                                                        >  2:1110836/1‑150 (MQ=255)
                                                                           cTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTtcatca                                                                      <  2:1644938/150‑1 (MQ=255)
                                                                             atttattCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAAc                                                                    >  1:331981/1‑150 (MQ=255)
                                                                              tttattCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACt                                                                   <  1:1699805/150‑1 (MQ=255)
                                                                                  ttCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAAt                                                               >  1:195012/1‑150 (MQ=255)
                                                                                    cAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGc                                                             >  1:950948/1‑150 (MQ=255)
                                                                                    cAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGc                                                             >  2:1543189/1‑150 (MQ=255)
                                                                                    cAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGc                                                             >  2:1565282/1‑150 (MQ=255)
                                                                                      aaaCTCTGACGAAAAACAGGCTGACGTCAGTTTTGACGTGACGAAACGAAATACAGCGAGACAGCCATCACGCCGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACACGCCGCCCAGCGATTTCATCAACTGAATGAtt                                                           >  1:1091605/1‑150 (MQ=255)
                                                                                         ctctGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTgg                                                        >  1:230198/1‑150 (MQ=255)
                                                                                           ctGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGAt                                                      <  1:454456/150‑1 (MQ=255)
                                                                                           ctGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGAt                                                      <  1:177276/150‑1 (MQ=255)
                                                                                            tGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATc                                                     >  1:960749/1‑150 (MQ=255)
                                                                                            tGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATc                                                     <  2:1470847/150‑1 (MQ=255)
                                                                                             gACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATCACCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCa                                                    >  1:158531/1‑150 (MQ=255)
                                                                                             gACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCa                                                    >  2:43881/1‑150 (MQ=255)
                                                                                                  aaaaCAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCcgg                                               >  2:531803/1‑150 (MQ=255)
                                                                                                      cAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCggcggc                                           <  1:718530/150‑1 (MQ=255)
                                                                                                      cAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCggcggc                                           <  1:1261806/150‑1 (MQ=255)
                                                                                                            gtcgtcAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCa                                     <  1:4861/150‑1 (MQ=255)
                                                                                                             gcgtcAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCCGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAg                                    <  1:307324/149‑1 (MQ=255)
                                                                                                              cgtcAGTTTTGACTTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGc                                   <  1:13596/150‑1 (MQ=255)
                                                                                                              cgtcAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGc                                   >  1:1273682/1‑150 (MQ=255)
                                                                                                               gtcAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCa                                  >  1:386750/1‑150 (MQ=255)
                                                                                                                 cAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATc                                <  1:1195062/150‑1 (MQ=255)
                                                                                                                 cAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCAGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATc                                <  1:1248023/150‑1 (MQ=255)
                                                                                                                   gTTTTGACGTGGCGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCac                              <  1:1717636/150‑2 (MQ=255)
                                                                                                                        gacgtgacgAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCATCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATg                         <  1:1134560/150‑1 (MQ=255)
                                                                                                                        gacgtgacgAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATg                         <  1:337040/150‑1 (MQ=255)
                                                                                                                          cgtgacgAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGAc                       <  1:1036362/150‑1 (MQ=255)
                                                                                                                           gtgacgAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACa                      <  1:1073733/150‑1 (MQ=255)
                                                                                                                               cgaaacgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACAtctc                  <  1:1543189/150‑1 (MQ=255)
                                                                                                                               cgaaacgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACAtctc                  >  2:1253899/1‑150 (MQ=255)
                                                                                                                                   acgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGCCATCTCTTCg              >  2:445149/1‑150 (MQ=255)
                                                                                                                                   acgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCg              >  1:504783/1‑150 (MQ=255)
                                                                                                                                   acgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCg              <  2:781009/150‑1 (MQ=255)
                                                                                                                                    cgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCgg             <  2:799056/150‑1 (MQ=255)
                                                                                                                                     gaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGc            <  2:684457/150‑1 (MQ=255)
                                                                                                                                       tctgccGCGTGATGGCTGTCACGCGGCAGACATTTTATTTTTTCTCGCCGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTTTCCAGCATCAGTAATGACATCTCTTCGGctt          >  1:877937/5‑148 (MQ=255)
                                                                                                                                        ctgccGCGTGATGGCTGTCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAg         >  1:676651/4‑150 (MQ=255)
                                                                                                                                          gccGCGTGATGGCTGTCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGACCAGCATCAGTAATGACAACTATTCGGAAAGcc       >  1:350971/2‑149 (MQ=255)
                                                                                                                                          aCCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATCCTTTCGATCACCCGGCGGCTGTCCAGCATCAGGAATGAAAGCTCTTCGGCTAGCa       >  2:1215704/1‑150 (MQ=255)
                                                                                                                                             gcgTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTg    <  1:690659/150‑1 (MQ=255)
                                                                                                                                              cgTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTgg   <  2:317921/150‑1 (MQ=255)
                                                                                                                                              cgTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTgg   >  1:1265165/1‑150 (MQ=255)
                                                                                                                                               gTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGc  <  2:1456260/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CATACGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGC  >  NC_000913/4299395‑4299687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: