Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,299,546 C→T 8.1% intergenic (‑180/+18) fdhF ← / ← mdtP formate dehydrogenase‑H, selenopolypeptide subunit/outer membrane factor of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,299,5460CT 8.1% 333.0 / 12.9 111intergenic (‑180/+18)fdhF/mdtPformate dehydrogenase‑H, selenopolypeptide subunit/outer membrane factor of efflux pump
Reads supporting (aligned to +/- strand):  ref base C (55/47);  new base T (3/6);  total (58/53)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

Only 100 of 115 total aligned reads displayed.

CGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGC  >  NC_000913/4299399‑4299695
                                                                                                                                                   |                                                                                                                                                     
cgcgCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCAt                                                                                                                                                     >  1:209695/1‑150 (MQ=255)
cgcgCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCAt                                                                                                                                                     >  2:4861/1‑150 (MQ=255)
cgcgCTCTCGACTACAGTATGCATCTTTTATGCCACATTTAATGTGGGGTCATTCCCTGATATTACGGGCACTAATTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCACTATTGACGTGACGAAACGCAATACCGCGTGACAGccct                                                                                                                                                     >  2:240630/1‑148 (MQ=255)
  cgcTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCa                                                                                                                                                   >  2:1460899/1‑150 (MQ=255)
    ctctCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAcg                                                                                                                                                 >  1:1456260/1‑150 (MQ=255)
      ctcGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCAcgcg                                                                                                                                               >  1:243597/1‑150 (MQ=255)
        cGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGATGGCTGTCACGCggt                                                                                                                                             <  2:920438/150‑2 (MQ=255)
        cGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCCCGCGGc                                                                                                                                             >  1:684457/1‑150 (MQ=255)
        cGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCAATCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGGCGGCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGc                                                                                                                                             <  1:1544036/150‑1 (MQ=255)
         gACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCAGAAATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGATGGCTGTCACGCggta                                                                                                                                            <  1:494220/150‑3 (MQ=255)
          aCTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTACCTGATATTACGGGCACTATTTATTCAAAACTCTGAAGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGATGGCTGTCACGCggtat                                                                                                                                           <  2:275861/150‑4 (MQ=255)
             aCAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGATGGCTGTCACGCggtatttc                                                                                                                                        <  1:1122811/150‑7 (MQ=255)
             aCAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACa                                                                                                                                        >  2:718530/1‑150 (MQ=255)
             aCAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACa                                                                                                                                        >  1:1307176/1‑150 (MQ=255)
              cAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAAAAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCCCGCGGCAGACat                                                                                                                                       >  2:1073733/1‑150 (MQ=255)
               aGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGGCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACatt                                                                                                                                      <  1:1044224/150‑1 (MQ=255)
                    gCATCTTTTATGCCACATTTTATGTTGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACattttatt                                                                                                                                 <  2:35315/150‑1 (MQ=255)
                    gCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACattttatt                                                                                                                                 <  2:29618/150‑1 (MQ=255)
                     cATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACattttattt                                                                                                                                >  2:643653/1‑150 (MQ=255)
                     cATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTAATCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACattttattt                                                                                                                                >  2:1279047/1‑150 (MQ=255)
                       tCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTAttttt                                                                                                                              >  2:1036362/1‑150 (MQ=255)
                       tCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTATTGACGTGACGAAACGAAATACCGCGTTACAGCCATCACGCGGCAGACATTTTAttttt                                                                                                                              >  1:1442652/1‑150 (MQ=255)
                           ttATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTtctc                                                                                                                          <  2:94297/150‑1 (MQ=255)
                               gCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCTTGACAGCCATCACGCGGCAGACATTTTATTTTTtcaagaca                                                                                                                      >  2:690659/1‑144 (MQ=255)
                               gCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcgacg                                                                                                                      >  2:199634/1‑150 (MQ=255)
                                ccACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTcgacga                                                                                                                     >  2:243690/1‑150 (MQ=255)
                                   caTTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACgg                                                                                                                  <  1:838566/150‑1 (MQ=255)
                                      tttATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAc                                                                                                               <  1:1540074/150‑1 (MQ=255)
                                      tttATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAc                                                                                                               <  1:132539/150‑1 (MQ=255)
                                       ttATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcc                                                                                                              >  2:1699805/1‑150 (MQ=255)
                                       ttATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcc                                                                                                              <  1:347137/150‑1 (MQ=255)
                                         aTGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcctg                                                                                                            <  2:375931/150‑1 (MQ=255)
                                           gtgGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcctgcc                                                                                                          <  1:484915/150‑1 (MQ=255)
                                           gtgGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcctgcc                                                                                                          <  1:891464/150‑1 (MQ=255)
                                             ggggTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGAcctgcctg                                                                                                        <  2:1276315/150‑1 (MQ=255)
                                                  cATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATAcc                                                                                                   >  2:1264709/1‑150 (MQ=255)
                                                  cATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATAcc                                                                                                   >  1:799056/1‑150 (MQ=255)
                                                   aTTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATAccc                                                                                                  >  2:714834/1‑150 (MQ=255)
                                                   aTTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATAccc                                                                                                  >  1:401246/1‑150 (MQ=255)
                                                   aTTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACAAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATGATTTCTCGACTACGGGCCCAGCCTGATAccc                                                                                                  >  1:125482/1‑150 (MQ=255)
                                                    ttCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACccg                                                                                                 >  2:1180638/1‑150 (MQ=255)
                                                     tCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATAACGCGGCAGACATTTTATTTTTTCTCGACGACGTGACCTGCCTGATACccgc                                                                                                >  2:1667171/1‑150 (MQ=255)
                                                      cccTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACccgcc                                                                                               <  2:708167/150‑1 (MQ=255)
                                                      cccTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACccgcc                                                                                               <  2:243597/150‑1 (MQ=255)
                                                      cccTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACccgcc                                                                                               >  2:177276/1‑150 (MQ=255)
                                                            tatTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATAACGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAg                                                                                         >  1:110438/1‑150 (MQ=255)
                                                             atTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGc                                                                                        >  1:176492/1‑150 (MQ=255)
                                                                 cgtgCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCCTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGAtt                                                                                    <  1:1026636/147‑1 (MQ=255)
                                                                  gggCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGAttt                                                                                   >  2:1004925/1‑150 (MQ=255)
                                                                   ggCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTtc                                                                                  >  1:543791/1‑150 (MQ=255)
                                                                    gCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGGCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTtca                                                                                 <  1:1590007/150‑1 (MQ=255)
                                                                       cTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTtcatca                                                                              <  2:1644938/150‑1 (MQ=255)
                                                                         atttattCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAAc                                                                            >  1:331981/1‑150 (MQ=255)
                                                                          tttattCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACt                                                                           <  1:1699805/150‑1 (MQ=255)
                                                                             attCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGaa                                                                        >  2:337040/1‑150 (MQ=255)
                                                                              ttCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAAt                                                                       >  1:195012/1‑150 (MQ=255)
                                                                                cAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGc                                                                     >  1:950948/1‑150 (MQ=255)
                                                                                cAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGc                                                                     >  2:1565282/1‑150 (MQ=255)
                                                                                cAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGc                                                                     >  2:1543189/1‑150 (MQ=255)
                                                                                  aaaCTCTGACGAAAAACAGGCTGACGTCAGTTTTGACGTGACGAAACGAAATACAGCGAGACAGCCATCACGCCGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACACGCCGCCCAGCGATTTCATCAACTGAATGAtt                                                                   >  1:1091605/1‑150 (MQ=255)
                                                                                     ctctGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTgg                                                                >  1:230198/1‑150 (MQ=255)
                                                                                       ctGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGAt                                                              <  1:454456/150‑1 (MQ=255)
                                                                                       ctGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGAt                                                              <  1:177276/150‑1 (MQ=255)
                                                                                        tGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATc                                                             >  1:960749/1‑150 (MQ=255)
                                                                                        tGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATc                                                             <  1:1681976/150‑1 (MQ=255)
                                                                                        tGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATc                                                             <  2:1470847/150‑1 (MQ=255)
                                                                                         gACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATCACCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCa                                                            >  1:158531/1‑150 (MQ=255)
                                                                                              aaaaCAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCcgg                                                       >  2:531803/1‑150 (MQ=255)
                                                                                                  cAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCggcggc                                                   <  1:718530/150‑1 (MQ=255)
                                                                                                  cAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCggcggc                                                   <  1:1261806/150‑1 (MQ=255)
                                                                                                        gtcgtcAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCa                                             <  1:4861/150‑1 (MQ=255)
                                                                                                         gcgtcAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCCGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAg                                            <  1:307324/149‑1 (MQ=255)
                                                                                                          cgtcAGTTTTGACTTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGc                                           <  1:13596/150‑1 (MQ=255)
                                                                                                          cgtcAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGc                                           >  1:1273682/1‑150 (MQ=255)
                                                                                                           gtcAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCa                                          >  1:386750/1‑150 (MQ=255)
                                                                                                             cAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATc                                        <  1:1195062/150‑1 (MQ=255)
                                                                                                             cAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCAGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATc                                        <  1:1248023/150‑1 (MQ=255)
                                                                                                                    gacgtgacgAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCATCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATg                                 <  1:1134560/150‑1 (MQ=255)
                                                                                                                    gacgtgacgAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATg                                 <  1:337040/150‑1 (MQ=255)
                                                                                                                      cgtgacgAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGAc                               <  1:1036362/150‑1 (MQ=255)
                                                                                                                       gtgacgAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACa                              <  1:1073733/150‑1 (MQ=255)
                                                                                                                           cgaaacgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACAtctc                          >  2:1253899/1‑150 (MQ=255)
                                                                                                                           cgaaacgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACAtctc                          <  1:1543189/150‑1 (MQ=255)
                                                                                                                               acgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGCCATCTCTTCg                      >  2:445149/1‑150 (MQ=255)
                                                                                                                               acgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCg                      <  2:781009/150‑1 (MQ=255)
                                                                                                                               acgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCg                      >  1:504783/1‑150 (MQ=255)
                                                                                                                                cgaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCgg                     <  2:799056/150‑1 (MQ=255)
                                                                                                                                 gaaaTACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGc                    <  2:684457/150‑1 (MQ=255)
                                                                                                                                    ctgccGCGTGATGGCTGTCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAg                 >  1:676651/4‑150 (MQ=255)
                                                                                                                                      gccGCGTGATGGCTGTCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGACCAGCATCAGTAATGACAACTATTCGGAAAGcc               >  1:350971/2‑149 (MQ=255)
                                                                                                                                      aCCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATCCTTTCGATCACCCGGCGGCTGTCCAGCATCAGGAATGAAAGCTCTTCGGCTAGCa               >  2:1215704/1‑150 (MQ=255)
                                                                                                                                        cgcgTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACt             >  1:1239013/1‑150 (MQ=255)
                                                                                                                                         gcgTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTg            <  1:690659/150‑1 (MQ=255)
                                                                                                                                          cgTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTgg           <  2:317921/150‑1 (MQ=255)
                                                                                                                                           gTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGc          <  2:1307176/150‑1 (MQ=255)
                                                                                                                                           gTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGc          <  1:710285/150‑1 (MQ=255)
                                                                                                                                                 gCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCgg    <  2:331981/150‑1 (MQ=255)
                                                                                                                                                 gCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCgg    >  2:241482/1‑150 (MQ=255)
                                                                                                                                                  ccATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTAGGATCACCCGGCGGCTGTCCAGCATAAGTAATGACATCTCTTCGGCAAGCACAGGCAACCggg   >  1:111058/1‑150 (MQ=255)
                                                                                                                                                   cATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGc  >  1:542734/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGCGCTCTCGACTACAGTATGCATCTTTTATGCCACATTTTATGTGGGGTCATTCCCTGATATTACGGGCACTATTTATTCAAAACTCTGACGAAAAACAGGCTGTCGTCAGTTTTGACGTGACGAAACGAAATACCGCGTGACAGCCATCACGCGGCAGACATTTTATTTTTTCTCGACGACGGGACCTGCCTGATACCCGCCGCCCAGCGATTTCATCAACTGAATGCTTTGGATCACCCGGCGGCTGTCCAGCATCAGTAATGACATCTCTTCGGCAAGCACTGGCAACCGGGC  >  NC_000913/4299399‑4299695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: