Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,315,439 A→T 6.9% F34I (TTT→ATT) ‡ phnO ← aminoalkylphosphonate N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,315,4390AT 6.9% 270.2 / 7.8 105F34I (TTT→ATT) ‡phnOaminoalkylphosphonate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (47/47);  new base T (5/2);  total (55/50)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01

Only 100 of 108 total aligned reads displayed.

CACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGACTGG  >  NC_000913/4315290‑4315588
                                                                                                                                                     |                                                                                                                                                     
caccaACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGaaa                                                                                                                                                       >  2:1647505/1‑150 (MQ=255)
  ccaACTCCTGAATTTCGCCGATCCAGCTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAAcg                                                                                                                                                     <  1:237585/150‑1 (MQ=255)
     aCTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgt                                                                                                                                                  <  1:192288/150‑1 (MQ=255)
        ccTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGTTTCGCgtggg                                                                                                                                               <  1:1114319/150‑2 (MQ=255)
        ccTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtggt                                                                                                                                               >  1:1618820/1‑150 (MQ=255)
        ccTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCAGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCCTTAAAACCCACGCGAAACGCgtggt                                                                                                                                               >  1:173906/1‑150 (MQ=255)
          tGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGTTTCGCgtgggtt                                                                                                                                             <  2:1253/150‑4 (MQ=255)
            aaTTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTc                                                                                                                                           <  2:298512/150‑1 (MQ=255)
             aTTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCa                                                                                                                                          <  2:1483744/150‑1 (MQ=255)
             aTTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCa                                                                                                                                          <  2:1627073/150‑1 (MQ=255)
             aTTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCa                                                                                                                                          >  1:1414476/1‑150 (MQ=255)
              tttCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCaa                                                                                                                                         <  1:723336/150‑1 (MQ=255)
                  gCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTc                                                                                                                                     >  1:831012/1‑150 (MQ=255)
                  gCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTc                                                                                                                                     >  1:1333504/1‑150 (MQ=255)
                      aTCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGcc                                                                                                                                 <  1:359305/150‑1 (MQ=255)
                         ccgTTGACATGATGCAGATGAAACTGCAAATGCAGGACGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGt                                                                                                                              <  2:1361082/148‑1 (MQ=255)
                          aGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGtt                                                                                                                             <  2:1618820/150‑1 (MQ=255)
                           gTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGCTTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGttt                                                                                                                            >  1:1392597/1‑150 (MQ=255)
                             tGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTa                                                                                                                          <  2:278906/150‑1 (MQ=255)
                              gACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAg                                                                                                                         <  2:917789/150‑1 (MQ=255)
                              gACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAg                                                                                                                         >  2:4664/1‑150 (MQ=255)
                               aCATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGc                                                                                                                        >  2:25393/1‑150 (MQ=255)
                               aCATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCCCATGTTTGGGTCGCGCAGATTGGCGTTTAAACCCACGCGAACCGCGTGGTGGTCAAACACCGCCTGTTTTAGc                                                                                                                        >  1:133161/1‑150 (MQ=255)
                                cATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCt                                                                                                                       >  1:294008/1‑150 (MQ=255)
                                        aGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAGCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATc                                                                                                               >  2:1265762/1‑150 (MQ=255)
                                            gAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAgcg                                                                                                           <  1:686558/150‑1 (MQ=255)
                                               aCTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGt                                                                                                        >  2:282965/1‑150 (MQ=255)
                                                cTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCACGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTa                                                                                                       <  1:960210/150‑1 (MQ=255)
                                                cTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTa                                                                                                       >  1:386315/1‑150 (MQ=255)
                                                 tGCAAATGCCGGCCTATCATGCCGACAACTTCGCCATCACCCAGCGCCAGATGGTCGCGCATGTTGGGGCCGCGCAGATTGGCGTTAAAACCCACGCTAACCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTaa                                                                                                      <  2:1440361/150‑1 (MQ=255)
                                                       tGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTGTTAGCTCACAAATCAGCGCGTAAACcgcg                                                                                                <  1:717614/150‑1 (MQ=255)
                                                         cAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTc                                                                                              >  2:475597/1‑150 (MQ=255)
                                                         cAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTc                                                                                              >  2:887918/1‑150 (MQ=255)
                                                         cAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTc                                                                                              >  2:513294/1‑150 (MQ=255)
                                                          aGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCg                                                                                             <  1:873829/150‑1 (MQ=255)
                                                           ggCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCgg                                                                                            >  2:1024259/1‑150 (MQ=255)
                                                           ggCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCgg                                                                                            >  2:685953/1‑150 (MQ=255)
                                                            gCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGCTTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTACCAAATCAGCGCGTAAACCGCGTCGgt                                                                                           >  1:1406502/1‑150 (MQ=255)
                                                            gCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGgt                                                                                           >  2:509722/1‑150 (MQ=255)
                                                            gCCGATCATACCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGgt                                                                                           >  2:605943/1‑150 (MQ=255)
                                                               gATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTc                                                                                        <  1:1368076/150‑1 (MQ=255)
                                                               gATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTc                                                                                        >  1:175028/1‑150 (MQ=255)
                                                                    tGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACt                                                                                   <  2:758853/150‑1 (MQ=255)
                                                                       cGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCg                                                                                <  1:1134354/150‑1 (MQ=255)
                                                                        gACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGt                                                                               <  2:902820/150‑1 (MQ=255)
                                                                           aaCTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGc                                                                            <  1:1091528/150‑1 (MQ=255)
                                                                            aCTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGcc                                                                           >  1:11397/1‑150 (MQ=255)
                                                                            aCTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGcc                                                                           >  1:184078/1‑150 (MQ=255)
                                                                               tCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCggg                                                                        >  2:1458660/1‑150 (MQ=255)
                                                                                   cATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGaa                                                                    >  2:627773/1‑150 (MQ=255)
                                                                                   cATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGGTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGaa                                                                    <  1:156294/150‑1 (MQ=255)
                                                                                    aTCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAg                                                                   <  1:1647505/150‑1 (MQ=255)
                                                                                     tCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGc                                                                  <  1:1565473/150‑1 (MQ=255)
                                                                                      cAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAACCGCGTGGTGGTCAACCTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt                                                                 >  1:172293/1‑150 (MQ=255)
                                                                                      cAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt                                                                 >  2:128597/1‑150 (MQ=255)
                                                                                        agcagcGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGATACGCGTGGTGGTCAAACTCCGCCTGGTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTca                                                               >  1:1276057/1‑150 (MQ=255)
                                                                                        agcagcGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTca                                                               >  2:725589/1‑150 (MQ=255)
                                                                                         gcagcGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGGCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTcac                                                              <  1:95002/150‑1 (MQ=255)
                                                                                            gcgcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAg                                                           <  1:661572/150‑1 (MQ=255)
                                                                                                cAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCTGGCGAAGCTCACAAGCAgg                                                       <  1:1428128/150‑1 (MQ=255)
                                                                                                cAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAgg                                                       <  2:586110/150‑1 (MQ=255)
                                                                                                     ggTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATgg                                                  <  1:366387/150‑1 (MQ=255)
                                                                                                      gTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGgt                                                 >  1:1263458/1‑150 (MQ=255)
                                                                                                          cgcATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGttt                                             >  2:601191/1‑150 (MQ=255)
                                                                                                           gcATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtc                                            <  1:920317/150‑1 (MQ=255)
                                                                                                            cATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtct                                           <  2:294008/150‑1 (MQ=255)
                                                                                                              tGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAGACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTcc                                         <  2:1661674/150‑1 (MQ=255)
                                                                                                               gTTTGGTGCGCGCAGATTGGCGTTAAAACCTACGCGATAAGAGTGGTGGTCAAACTCCGCCTGGTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGTCGAAGCTCACAAGCAGGCATGGTGTTTCTCCt                                        <  1:1065764/150‑1 (MQ=255)
                                                                                                               gTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCt                                        <  1:475597/150‑1 (MQ=255)
                                                                                                               gTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCt                                        >  1:1009090/1‑150 (MQ=255)
                                                                                                                  tGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCt                                     <  2:1720641/150‑1 (MQ=255)
                                                                                                                     gTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGAt                                  <  2:367192/150‑1 (MQ=255)
                                                                                                                     gTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGAt                                  <  2:361428/150‑1 (MQ=255)
                                                                                                                     gTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGAt                                  >  2:717026/1‑150 (MQ=255)
                                                                                                                      tCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATg                                 <  2:544330/150‑1 (MQ=255)
                                                                                                                      gcgcgCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATg                                 <  1:232523/149‑1 (MQ=255)
                                                                                                                        gcgcAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGa                               <  2:1380363/150‑1 (MQ=255)
                                                                                                                            aGATTGGCGTTAAAACCCACGCGAAACGCGGGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGGAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAg                           <  1:938952/150‑1 (MQ=255)
                                                                                                                              aTTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCg                         >  2:1244067/1‑150 (MQ=255)
                                                                                                                              aTTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCg                         >  2:1120229/1‑150 (MQ=255)
                                                                                                                                 ggCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTca                      >  2:626990/1‑150 (MQ=255)
                                                                                                                                 ggCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGACCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTACTTCTGATGGATCAGCGTca                      >  1:949852/1‑150 (MQ=255)
                                                                                                                                   cGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTcagc                    <  1:586184/150‑1 (MQ=255)
                                                                                                                                    gTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTcagca                   >  2:1731319/1‑150 (MQ=255)
                                                                                                                                     ttAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTcagcag                  >  1:1374989/1‑150 (MQ=255)
                                                                                                                                     ttAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTcagcag                  >  1:1280223/1‑150 (MQ=255)
                                                                                                                                       gaccaccACGCGTTTCGCGTGGTGGTCAAACTCCGCCTGTATTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCGTTCTGATGGATCAGCGTCAGCAGCg                >  1:150021/6‑150 (MQ=255)
                                                                                                                                       aaaaCCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCg                <  1:509722/150‑1 (MQ=255)
                                                                                                                                        accaccACGCGTTTCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCgt               >  1:1141711/5‑150 (MQ=255)
                                                                                                                                         aaCCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTATCCTTCTGATGGATCAGCGTCAGCAGCgtg              >  2:1039047/1‑150 (MQ=255)
                                                                                                                                            ccACGCGTTTCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcg           >  1:944046/1‑150 (MQ=255)
                                                                                                                                            ccACGCGTTTCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcg           >  1:1567581/1‑150 (MQ=255)
                                                                                                                                            ccACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcg           <  2:398836/150‑1 (MQ=255)
                                                                                                                                            ccACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcg           >  1:1454572/1‑150 (MQ=255)
                                                                                                                                              aCGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgac         <  1:1024259/150‑1 (MQ=255)
                                                                                                                                               cgcgAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTcgacc        >  2:1732033/1‑150 (MQ=255)
                                                                                                                                               cgcgAAACGCGTGGTGGGCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGGcgacc        <  1:948311/150‑1 (MQ=255)
                                                                                                                                                gcgAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAACCAGGCATGGTGTATCTCCTTCTGATGGATCAGAGTCAGCAGCGTGTcgaccg       >  1:142721/1‑150 (MQ=255)
                                                                                                                                                    aaCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGGGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGACTg   <  1:865629/150‑1 (MQ=255)
                                                                                                                                                     aCGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGACTgg  <  2:175028/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCCTTCTGATGGATCAGCGTCAGCAGCGTGTCGACCGACTGG  >  NC_000913/4315290‑4315588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: