Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,332,529 C→G 5.6% Q248H (CAG→CAC) ‡ basS ← sensory histidine kinase in two‑component regulatory system with BasR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,5290CG 5.6% 432.8 / 14.7 144Q248H (CAG→CAC) ‡basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base C (77/59);  new base G (3/5);  total (80/64)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01

Only 100 of 146 total aligned reads displayed.

CATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGC  >  NC_000913/4332381‑4332678
                                                                                                                                                    |                                                                                                                                                     
catcaATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCt                                                                                                                                                      >  2:1697632/1‑150 (MQ=255)
    aaTACCTGGTCCTTCCTCTTCAACTGCCATGAACGCTCCGTCATCTTATTGCAGCTTAATCATAATCTTGCTGACTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCATCAGTGTTTCATCACCCTGAAc                                                                                                                                                  >  1:206537/1‑150 (MQ=255)
     aTACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCCCCCTGAACg                                                                                                                                                 >  2:1591803/1‑150 (MQ=255)
     aTACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACg                                                                                                                                                 >  2:404142/1‑150 (MQ=255)
        ccTGGTCCTTCCTCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCATCTTAATCATAATGTTGCTGCCTTGCGGGATGGAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCAGAAGCAGGGCTGCATCACCCTGAACGGTg                                                                                                                                              <  2:1369133/150‑1 (MQ=255)
        ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                              >  1:1704866/1‑150 (MQ=255)
        ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                              >  1:1513898/1‑150 (MQ=255)
        ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                              >  2:649234/1‑150 (MQ=255)
        ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                              <  1:1635924/150‑1 (MQ=255)
        ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                              <  2:894997/150‑1 (MQ=255)
         cTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGa                                                                                                                                             <  1:332468/150‑1 (MQ=255)
          tGGTCCTTCATCTTCAACAGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGTTTGCATCACCCTGAACGGTGAt                                                                                                                                            <  1:1182054/150‑1 (MQ=255)
           ggTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATg                                                                                                                                           <  2:409735/150‑1 (MQ=255)
              ccTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCAGCCTGAACGGTGATGTcg                                                                                                                                        >  2:586811/1‑150 (MQ=255)
              ccTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCGTTCAGGGTGATgcaa                                                                                                                                        <  1:671770/150‑4 (MQ=255)
              ccTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTCGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcg                                                                                                                                        >  2:818266/1‑150 (MQ=255)
               cTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTACATCACCGTTCAGGGTGATgcaac                                                                                                                                       <  1:1250605/150‑5 (MQ=255)
                ttCATCTTCAACTGCCATGCCCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGAGGGCTGTAACGATGGGCGTTTTCTACCATGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCGTTCAGGGTGATgcaacc                                                                                                                                      <  2:1190994/150‑6 (MQ=255)
                ttCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcggc                                                                                                                                      >  2:1437662/1‑150 (MQ=255)
                ttCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTACATCACCGTTCAGGGTGATgcaacc                                                                                                                                      <  1:555218/150‑6 (MQ=255)
                   aTCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCggcggc                                                                                                                                   >  1:1537207/1‑150 (MQ=255)
                     cTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGgcgc                                                                                                                                 >  2:431134/1‑150 (MQ=255)
                     cTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACACTGAACGGTGATGTCGGCGgcgc                                                                                                                                 >  2:393862/1‑150 (MQ=255)
                          aCTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctctct                                                                                                                            >  2:341760/1‑150 (MQ=255)
                           cTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTg                                                                                                                           <  1:40280/150‑1 (MQ=255)
                           cTGCCATGACCGCGCCGTTATCTTCTTGCAGGTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTTATGTCGGCGGCGCTCTCTg                                                                                                                           <  2:1409574/150‑1 (MQ=255)
                             gCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                         >  1:663502/1‑150 (MQ=255)
                             gCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGAGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                         >  2:1048889/1‑150 (MQ=255)
                             gCCAGGACCGCTCCGTCATCTTCTTGCAGCTTAATCAAAATGTTGCTGCCTTGCGGGCGGTAACGATGGGCGCTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                         <  1:282595/150‑1 (MQ=255)
                               cATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGGGATGTCGGCGGCGCTCTCTGGCaa                                                                                                                       >  2:1235979/1‑150 (MQ=255)
                                  gACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAg                                                                                                                    <  1:163097/150‑1 (MQ=255)
                                  gACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGAGGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCCGGGTTGCATCCGCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAg                                                                                                                    >  2:84075/1‑150 (MQ=255)
                                    ccGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCa                                                                                                                  >  1:1599846/1‑150 (MQ=255)
                                         ccGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGtt                                                                                                             >  1:418177/1‑150 (MQ=255)
                                         ccGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGAAGGGtt                                                                                                             >  1:1090748/1‑150 (MQ=255)
                                            tCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGc                                                                                                          <  1:1031242/150‑1 (MQ=255)
                                               tcttctTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCTGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGt                                                                                                       >  2:1035136/1‑150 (MQ=255)
                                                        cgCTAAATCATAATTGTGCTGCCTTGCGGGCTGTAACGATGGGAGTTTTATAACAGGTTTCGCAATAACATCCGCAGCAGGGCTGCATCACCCTGAACGGTGATGCCGGCGGCGCTCTCTGGCAATCGCAGGGTTTGCTGTCGCTGGGCg                                                                                              <  2:1439727/149‑1 (MQ=255)
                                                           ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCGTCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGc                                                                                           <  2:1510708/150‑1 (MQ=255)
                                                           ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGc                                                                                           <  1:1188401/150‑1 (MQ=255)
                                                             aaTCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCAt                                                                                         >  1:1002258/1‑150 (MQ=255)
                                                                cATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGt                                                                                      <  2:1185748/150‑1 (MQ=255)
                                                                     tGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGa                                                                                 >  1:1297121/1‑150 (MQ=255)
                                                                      gTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAg                                                                                >  2:852165/1‑150 (MQ=255)
                                                                      gTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAg                                                                                >  1:1242484/1‑150 (MQ=255)
                                                                        tgctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCATGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGtt                                                                              >  1:346604/1‑150 (MQ=255)
                                                                              cTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCGCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCAt                                                                        <  1:1543439/150‑1 (MQ=255)
                                                                                 gCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGGCGCTGGTCGAGCATGGTACTGAGTTCGTCATACg                                                                     <  2:1120527/150‑1 (MQ=255)
                                                                                 gCGGGCTGTAACGATGGGCGGTTTCTACCAGCTTTCGCAATAACAACCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACg                                                                     <  2:611350/150‑1 (MQ=255)
                                                                                  cGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATATCATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCTGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATGCgg                                                                    >  2:266099/1‑149 (MQ=255)
                                                                                  cGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGa                                                                    >  1:1210867/1‑150 (MQ=255)
                                                                                    ggCTGTAACGATGGGCGTTTTATACAAGGTTACGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGTGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAgg                                                                  >  1:1308267/1‑150 (MQ=255)
                                                                                        gTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGgag                                                              >  1:1643511/1‑150 (MQ=255)
                                                                                          aaCGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGaa                                                            >  2:1191910/1‑150 (MQ=255)
                                                                                          aaCGATGGGCGTTTTCTACCAGGTTTCGCAATAACATACGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGaa                                                            >  1:924667/1‑150 (MQ=255)
                                                                                            cGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATg                                                          >  1:682644/1‑150 (MQ=255)
                                                                                             gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATg                                                          <  2:440130/149‑1 (MQ=255)
                                                                                             gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATg                                                          >  1:440130/1‑149 (MQ=255)
                                                                                               tGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACa                                                       <  2:1513898/150‑1 (MQ=255)
                                                                                                    gTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCGCATGCAATAGCAGGGTTTGCTGTCGCTTGTCGAGCATGGGACTGAGTTCGTCATACGAGGGGAGAATGACATCTTc                                                  <  2:260959/150‑1 (MQ=255)
                                                                                                       ttCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcag                                               <  1:685742/150‑1 (MQ=255)
                                                                                                         cTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                             >  2:911342/1‑150 (MQ=255)
                                                                                                         cTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                             >  1:935081/1‑150 (MQ=255)
                                                                                                         cTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                             <  1:365698/150‑1 (MQ=255)
                                                                                                            gcAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACACATAACTGTGATGTCGGCTGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGATCATGGTACTCAGTTCGTCATACGAGCTGAGAATGACATATTCcagaaatt                                          >  1:238491/2‑145 (MQ=255)
                                                                                                            ccAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCAGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGCCATCTTCCAGCAGtt                                          >  1:238489/1‑150 (MQ=255)
                                                                                                             cAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGATTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGttt                                         >  1:633671/1‑150 (MQ=255)
                                                                                                              ccgcgtCGCAATAACATCCCCAGCAGGGTGGCATCACCCTGAACGGTGATGTCGGCGGCGCGCTCTGCCCATAGCAGGGTTTGCTGTCGCTGGGCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                        <  2:1648059/145‑1 (MQ=255)
                                                                                                              aGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                        <  1:1227315/150‑1 (MQ=255)
                                                                                                              aGGGTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                        <  1:1048889/150‑1 (MQ=255)
                                                                                                                  ttCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACAt                                    >  2:510309/1‑150 (MQ=255)
                                                                                                                    cGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGt                                  <  1:404142/150‑1 (MQ=255)
                                                                                                                     gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                 <  2:1514127/150‑1 (MQ=255)
                                                                                                                     gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                 >  1:1410900/1‑150 (MQ=255)
                                                                                                                      cacaaaCATCCGCACCAGCGCTGCATCACCCTGAACGGGGATGTCGGCGGCGCGCTCGCGCAATAGCAGGGTCTGCTGTCGCTGGTCGAGCATGGTACTGAGGTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTg                                <  2:230385/146‑1 (MQ=255)
                                                                                                                      cAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTg                                >  1:519627/1‑150 (MQ=255)
                                                                                                                         tAACATCCGCCGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGTGTTAGCTGTCGCGGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAAGGACATCTTCCAGCAGTTTTACATGTTGATa                             <  2:1127753/150‑1 (MQ=255)
                                                                                                                         tAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATa                             <  1:643013/150‑1 (MQ=255)
                                                                                                                          aaCATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATaa                            >  1:1062972/1‑150 (MQ=255)
                                                                                                                             aTCCGCCGCAGGGGTGCATCTCCCTGAACGGTGATGTCGGCGGCGCTCTCTGTCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACAGAGTTCGTCATACGAGGGGAGAAAGACAGCTTCCAGCAGTTTTACATGTTGATAAttc                         <  1:1622220/150‑2 (MQ=255)
                                                                                                                              tCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTAc                        <  2:1242484/150‑1 (MQ=255)
                                                                                                                               ccGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTAcc                       <  2:1479283/150‑1 (MQ=255)
                                                                                                                               ccGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTAcc                       <  1:1697632/150‑1 (MQ=255)
                                                                                                                                  cagcagGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGga                    >  1:198355/1‑150 (MQ=255)
                                                                                                                                     cagGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaaga                 >  1:789321/1‑150 (MQ=255)
                                                                                                                                     cagGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaaga                 <  2:1712026/150‑1 (MQ=255)
                                                                                                                                       gggTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaa               >  1:1280804/1‑150 (MQ=255)
                                                                                                                                       gggTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaa               >  1:1501133/1‑150 (MQ=255)
                                                                                                                                         gTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaag                  >  1:794780/1‑145 (MQ=255)
                                                                                                                                         gTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAt             <  1:818266/150‑1 (MQ=255)
                                                                                                                                         ccgacATCACCGTTCAGGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAt             >  1:1654442/5‑150 (MQ=255)
                                                                                                                                           tGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa           >  2:811967/1‑150 (MQ=255)
                                                                                                                                           tGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTAGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa           >  1:995676/1‑150 (MQ=255)
                                                                                                                                            gCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGAc          <  2:1670385/150‑1 (MQ=255)
                                                                                                                                              aTCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTg        <  2:1601792/150‑1 (MQ=255)
                                                                                                                                               tCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGt       <  1:445611/150‑1 (MQ=255)
                                                                                                                                                cACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTc      >  2:1070954/1‑150 (MQ=255)
                                                                                                                                                 aCCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTcc     >  1:1381737/1‑150 (MQ=255)
                                                                                                                                                  cccTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCg    >  1:1581393/1‑150 (MQ=255)
                                                                                                                                                    cTGAACGGTGATGTCGGCGGCGCGCTCTGGCAATAGCAGGGTTTGCGGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGc  <  2:782150/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CATCAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGC  >  NC_000913/4332381‑4332678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑