Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,332,531 G→T 6.0% Q248K (CAG→AAG) ‡ basS ← sensory histidine kinase in two‑component regulatory system with BasR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,5310GT 6.0% 434.6 / 11.8 145Q248K (CAG→AAG) ‡basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base G (76/60);  new base T (4/5);  total (80/65)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01

Only 100 of 147 total aligned reads displayed.

CAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCAC  >  NC_000913/4332384‑4332680
                                                                                                                                                   |                                                                                                                                                     
  aTACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCCCCCTGAACg                                                                                                                                                   >  2:1591803/1‑150 (MQ=255)
     ccTGGTCCTTCCTCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCATCTTAATCATAATGTTGCTGCCTTGCGGGATGGAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCAGAAGCAGGGCTGCATCACCCTGAACGGTg                                                                                                                                                <  2:1369133/150‑1 (MQ=255)
     ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                                <  2:894997/150‑1 (MQ=255)
     ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                                <  1:1635924/150‑1 (MQ=255)
     ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                                >  1:1513898/1‑150 (MQ=255)
     ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                                >  2:649234/1‑150 (MQ=255)
      cTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGa                                                                                                                                               <  1:332468/150‑1 (MQ=255)
       tGGTCCTTCATCTTCAACAGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGTTTGCATCACCCTGAACGGTGAt                                                                                                                                              <  1:1182054/150‑1 (MQ=255)
        ggTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATg                                                                                                                                             <  2:409735/150‑1 (MQ=255)
           ccTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCAGCCTGAACGGTGATGTcg                                                                                                                                          >  2:586811/1‑150 (MQ=255)
           ccTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCGTTCAGGGTGATgcaa                                                                                                                                          <  1:671770/150‑4 (MQ=255)
           ccTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTCGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcg                                                                                                                                          >  2:818266/1‑150 (MQ=255)
            cTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCGTTCAGGGTGATgcaac                                                                                                                                         <  1:680192/150‑5 (MQ=255)
            cTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTACATCACCGTTCAGGGTGATgcaac                                                                                                                                         <  1:1250605/150‑5 (MQ=255)
             ttCATCTTCAACTGCCATGCCCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGAGGGCTGTAACGATGGGCGTTTTCTACCATGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCGTTCAGGGTGATgcaacc                                                                                                                                        <  2:1190994/150‑6 (MQ=255)
             ttCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcggc                                                                                                                                        >  2:1437662/1‑150 (MQ=255)
             ttCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTACATCACCGTTCAGGGTGATgcaacc                                                                                                                                        <  1:555218/150‑6 (MQ=255)
                aTCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCggcggc                                                                                                                                     >  1:1537207/1‑150 (MQ=255)
                  cTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACACTGAACGGTGATGTCGGCGgcgc                                                                                                                                   >  2:393862/1‑150 (MQ=255)
                       aCTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctctct                                                                                                                              >  2:341760/1‑150 (MQ=255)
                        cTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTg                                                                                                                             <  1:40280/150‑1 (MQ=255)
                        cTGCCATGACCGCGCCGTTATCTTCTTGCAGGTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTTATGTCGGCGGCGCTCTCTg                                                                                                                             <  2:1409574/150‑1 (MQ=255)
                          gCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                           <  2:403911/150‑1 (MQ=255)
                          gCCAGGACCGCTCCGTCATCTTCTTGCAGCTTAATCAAAATGTTGCTGCCTTGCGGGCGGTAACGATGGGCGCTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                           <  1:282595/150‑1 (MQ=255)
                            cATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGGGATGTCGGCGGCGCTCTCTGGCaa                                                                                                                         >  2:1235979/1‑150 (MQ=255)
                               gACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAg                                                                                                                      <  1:163097/150‑1 (MQ=255)
                               gACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGAGGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCCGGGTTGCATCCGCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAg                                                                                                                      >  2:84075/1‑150 (MQ=255)
                                 ccGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCa                                                                                                                    >  1:1599846/1‑150 (MQ=255)
                                      ccGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGAAGGGtt                                                                                                               >  1:1090748/1‑150 (MQ=255)
                                         tCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGc                                                                                                            <  1:1031242/150‑1 (MQ=255)
                                           aTCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGTTGGGCGTTTTCTACCAGGTTTCGCTATAACATCCGCTGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTg                                                                                                          <  2:136147/150‑1 (MQ=255)
                                            tcttctTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCTGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGt                                                                                                         >  2:1035136/1‑150 (MQ=255)
                                                     cgCTAAATCATAATTGTGCTGCCTTGCGGGCTGTAACGATGGGAGTTTTATAACAGGTTTCGCAATAACATCCGCAGCAGGGCTGCATCACCCTGAACGGTGATGCCGGCGGCGCTCTCTGGCAATCGCAGGGTTTGCTGTCGCTGGGCg                                                                                                <  2:1439727/149‑1 (MQ=255)
                                                      gCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGa                                                                                               >  1:829835/1‑150 (MQ=255)
                                                        ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCGTCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGc                                                                                             <  2:1510708/150‑1 (MQ=255)
                                                        ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGc                                                                                             <  1:1188401/150‑1 (MQ=255)
                                                          aaTCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCAt                                                                                           >  1:1002258/1‑150 (MQ=255)
                                                             cATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGt                                                                                        >  1:743828/1‑150 (MQ=255)
                                                              aTAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTa                                                                                       >  2:1178671/1‑150 (MQ=255)
                                                                  tGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGa                                                                                   >  1:1297121/1‑150 (MQ=255)
                                                                   gTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAg                                                                                  >  2:852165/1‑150 (MQ=255)
                                                                   gTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAg                                                                                  >  1:1242484/1‑150 (MQ=255)
                                                                   gTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAg                                                                                  >  1:1670385/1‑150 (MQ=255)
                                                                     tgctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCATGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGtt                                                                                >  1:346604/1‑150 (MQ=255)
                                                                          ccTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCa                                                                           >  1:1705360/1‑150 (MQ=255)
                                                                           cTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCGCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCAt                                                                          <  1:1543439/150‑1 (MQ=255)
                                                                              gCGGGCTGTAACGATGGGCGGTTTCTACCAGCTTTCGCAATAACAACCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACg                                                                       <  2:611350/150‑1 (MQ=255)
                                                                               cGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATATCATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCTGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATGCgg                                                                      >  2:266099/1‑149 (MQ=255)
                                                                               cGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGa                                                                      >  1:1210867/1‑150 (MQ=255)
                                                                               cGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGa                                                                      >  1:271201/1‑150 (MQ=255)
                                                                                     gTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGgag                                                                >  1:1643511/1‑150 (MQ=255)
                                                                                       aaCGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGaa                                                              >  2:1191910/1‑150 (MQ=255)
                                                                                        aCGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCACTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGAACTGAGTTCGTCATACGAGGGGATAAt                                                             >  1:136124/1‑150 (MQ=255)
                                                                                          gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATg                                                            <  2:440130/149‑1 (MQ=255)
                                                                                          gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATg                                                            >  1:440130/1‑149 (MQ=255)
                                                                                            tGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACa                                                         <  2:1513898/150‑1 (MQ=255)
                                                                                             gggCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACAt                                                        >  1:51392/1‑150 (MQ=255)
                                                                                               gCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCt                                                      >  2:428215/1‑150 (MQ=255)
                                                                                                 gTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCGCATGCAATAGCAGGGTTTGCTGTCGCTTGTCGAGCATGGGACTGAGTTCGTCATACGAGGGGAGAATGACATCTTc                                                    <  2:260959/150‑1 (MQ=255)
                                                                                                    ttCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcag                                                 <  1:685742/150‑1 (MQ=255)
                                                                                                      cTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                               >  1:935081/1‑150 (MQ=255)
                                                                                                      cTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                               <  1:365698/150‑1 (MQ=255)
                                                                                                      cTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                               >  2:911342/1‑150 (MQ=255)
                                                                                                       tACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagcag                                              >  1:425939/1‑150 (MQ=255)
                                                                                                         ccAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCAGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGCCATCTTCCAGCAGtt                                            >  1:238489/1‑150 (MQ=255)
                                                                                                           aGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                          <  1:1227315/150‑1 (MQ=255)
                                                                                                           aGGGTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                          <  1:1048889/150‑1 (MQ=255)
                                                                                                               ttCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACAt                                      >  2:510309/1‑150 (MQ=255)
                                                                                                                 cGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGt                                    <  1:404142/150‑1 (MQ=255)
                                                                                                                  gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                   >  1:966769/1‑150 (MQ=255)
                                                                                                                  gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                   >  1:647324/1‑150 (MQ=255)
                                                                                                                  gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                   <  2:1514127/150‑1 (MQ=255)
                                                                                                                  gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGAGATGTCGGCGGCGCTCACTGGCAATAGCAGGGTTTGCTGTCGCAGGTCGAGCATGGTACAGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTAACATGtt                                   >  1:234455/1‑150 (MQ=255)
                                                                                                                  gCAATAACATCCGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCGGTCGCTGGTCGAGCATGGTACTGAGCTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                   <  2:1048076/150‑1 (MQ=255)
                                                                                                                   cacaaaCATCCGCACCAGCGCTGCATCACCCTGAACGGGGATGTCGGCGGCGCGCTCGCGCAATAGCAGGGTCTGCTGTCGCTGGTCGAGCATGGTACTGAGGTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTg                                  <  2:230385/146‑1 (MQ=255)
                                                                                                                   cAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTg                                  >  1:519627/1‑150 (MQ=255)
                                                                                                                      tAACATCCGCCGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGTGTTAGCTGTCGCGGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAAGGACATCTTCCAGCAGTTTTACATGTTGATa                               <  2:1127753/150‑1 (MQ=255)
                                                                                                                      tAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATa                               <  1:643013/150‑1 (MQ=255)
                                                                                                                       aaCATCCTCAGCAGGGTTGCATCACGCTGAACGTTGATGTCGGCGGCGCTCTCTGGCTATAGCAGGGTTTGCTGTCTCTGGTCGAGCATGGTACTGAGTTCGTCATACGATGGGAGAATGTCATCTTCCAGCATTTTTACATGTTGATaa                              <  1:588831/150‑1 (MQ=255)
                                                                                                                       aaCATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATaa                              >  1:1062972/1‑150 (MQ=255)
                                                                                                                          aTCCGCCGCAGGGGTGCATCTCCCTGAACGGTGATGTCGGCGGCGCTCTCTGTCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACAGAGTTCGTCATACGAGGGGAGAAAGACAGCTTCCAGCAGTTTTACATGTTGATAAttc                           <  1:1622220/150‑2 (MQ=255)
                                                                                                                          aTCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGGTTTACATGTTGATAtttt                           >  2:1089696/1‑149 (MQ=255)
                                                                                                                           tCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTAc                          <  2:1242484/150‑1 (MQ=255)
                                                                                                                            ccGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTAcc                         <  2:1479283/150‑1 (MQ=255)
                                                                                                                            ccGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTAcc                         <  1:1697632/150‑1 (MQ=255)
                                                                                                                               cagcagGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGga                      >  1:198355/1‑150 (MQ=255)
                                                                                                                                  cagGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaaga                   >  1:789321/1‑150 (MQ=255)
                                                                                                                                  cagGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaaga                   <  2:1712026/150‑1 (MQ=255)
                                                                                                                                   ccgccgacATCACCGTTCAGGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaagaa                  >  2:1669213/8‑150 (MQ=255)
                                                                                                                                    gggTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaa                 >  1:1280804/1‑150 (MQ=255)
                                                                                                                                    gggTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaa                 >  1:1501133/1‑150 (MQ=255)
                                                                                                                                      gTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAt               <  1:818266/150‑1 (MQ=255)
                                                                                                                                        tGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa             >  2:811967/1‑150 (MQ=255)
                                                                                                                                         gCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGAc            <  2:1670385/150‑1 (MQ=255)
                                                                                                                                           aTCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTg          <  2:1601792/150‑1 (MQ=255)
                                                                                                                                             cACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTc        >  2:1070954/1‑150 (MQ=255)
                                                                                                                                              aCCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTcc       >  1:1381737/1‑150 (MQ=255)
                                                                                                                                              aCCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTcc       <  1:1458541/150‑1 (MQ=255)
                                                                                                                                               cccTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCg      >  1:1581393/1‑150 (MQ=255)
                                                                                                                                                 cTGAACGGTGATGTCGGCGGCGCGCTCTGGCAATAGCAGGGTTTGCGGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGc    <  2:782150/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CAATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCAC  >  NC_000913/4332384‑4332680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: