Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,332,534 C→G 5.5% V247L (GTT→CTT) ‡ basS ← sensory histidine kinase in two‑component regulatory system with BasR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,332,5340CG 5.5% 447.5 / 14.2 145V247L (GTT→CTT) ‡basSsensory histidine kinase in two‑component regulatory system with BasR
Reads supporting (aligned to +/- strand):  ref base C (76/61);  new base G (3/5);  total (79/66)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-01

Only 100 of 146 total aligned reads displayed.

AATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCAC  >  NC_000913/4332385‑4332680
                                                                                                                                                     |                                                                                                                                                  
aaTACCTGGTCCTTCCTCTTCAACTGCCATGAACGCTCCGTCATCTTATTGCAGCTTAATCATAATCTTGCTGACTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCATCAGTGTTTCATCACCCTGAAc                                                                                                                                                    >  1:206537/1‑150 (MQ=255)
 aTACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCCCCCTGAACg                                                                                                                                                   >  2:1591803/1‑150 (MQ=255)
 aTACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACg                                                                                                                                                   >  2:404142/1‑150 (MQ=255)
    ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                                >  1:1704866/1‑150 (MQ=255)
    ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                                >  1:1513898/1‑150 (MQ=255)
    ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                                >  2:649234/1‑150 (MQ=255)
    ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                                <  1:1635924/150‑1 (MQ=255)
    ccTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTg                                                                                                                                                <  2:894997/150‑1 (MQ=255)
     cTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGa                                                                                                                                               <  1:332468/150‑1 (MQ=255)
      tGGTCCTTCATCTTCAACAGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGTTTGCATCACCCTGAACGGTGAt                                                                                                                                              <  1:1182054/150‑1 (MQ=255)
          ccTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCAGCCTGAACGGTGATGTcg                                                                                                                                          >  2:586811/1‑150 (MQ=255)
           cTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCGTTCAGGGTGATgcaac                                                                                                                                         <  1:680192/150‑5 (MQ=255)
           cTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTcgg                                                                                                                                         >  2:643013/1‑150 (MQ=255)
           cTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTACATCACCGTTCAGGGTGATgcaac                                                                                                                                         <  1:1250605/150‑5 (MQ=255)
            ttCATCTTCAACTGCCATGCCCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGAGGGCTGTAACGATGGGCGTTTTCTACCATGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCGTTCAGGGTGATgcaacc                                                                                                                                        <  2:1190994/150‑6 (MQ=255)
               aTCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCggcggc                                                                                                                                     >  1:1537207/1‑150 (MQ=255)
                 cTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGgcgc                                                                                                                                   >  2:431134/1‑150 (MQ=255)
                      aCTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGctctct                                                                                                                              >  2:341760/1‑150 (MQ=255)
                       cTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTg                                                                                                                             <  1:40280/150‑1 (MQ=255)
                       cTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTg                                                                                                                             >  2:1227315/1‑150 (MQ=255)
                       cTGCCATGACCGCGCCGTTATCTTCTTGCAGGTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTTATGTCGGCGGCGCTCTCTg                                                                                                                             <  2:1409574/150‑1 (MQ=255)
                         gCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                           <  2:403911/150‑1 (MQ=255)
                         gCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                           >  1:663502/1‑150 (MQ=255)
                         gCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGAGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                           >  2:1048889/1‑150 (MQ=255)
                         gCCAGGACCGCTCCGTCATCTTCTTGCAGCTTAATCAAAATGTTGCTGCCTTGCGGGCGGTAACGATGGGCGCTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGc                                                                                                                           <  1:282595/150‑1 (MQ=255)
                           cATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGGGATGTCGGCGGCGCTCTCTGGCaa                                                                                                                         >  2:1235979/1‑150 (MQ=255)
                              gACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAg                                                                                                                      <  1:163097/150‑1 (MQ=255)
                              gACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGAGGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCCGGGTTGCATCCGCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAg                                                                                                                      >  2:84075/1‑150 (MQ=255)
                                ccGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCa                                                                                                                    >  1:1599846/1‑150 (MQ=255)
                                     ccGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGtt                                                                                                               >  1:418177/1‑150 (MQ=255)
                                     ccGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGAAGGGtt                                                                                                               >  1:1090748/1‑150 (MQ=255)
                                        tCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGc                                                                                                            <  1:1031242/150‑1 (MQ=255)
                                          aTCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGTTGGGCGTTTTCTACCAGGTTTCGCTATAACATCCGCTGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTg                                                                                                          <  2:136147/150‑1 (MQ=255)
                                          aTCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTg                                                                                                          >  2:1458541/1‑150 (MQ=255)
                                                    cgCTAAATCATAATTGTGCTGCCTTGCGGGCTGTAACGATGGGAGTTTTATAACAGGTTTCGCAATAACATCCGCAGCAGGGCTGCATCACCCTGAACGGTGATGCCGGCGGCGCTCTCTGGCAATCGCAGGGTTTGCTGTCGCTGGGCg                                                                                                <  2:1439727/149‑1 (MQ=255)
                                                    aGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCg                                                                                                <  2:1704866/150‑1 (MQ=255)
                                                     gCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGa                                                                                               >  1:829835/1‑150 (MQ=255)
                                                       ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCGTCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGc                                                                                             <  2:1510708/150‑1 (MQ=255)
                                                       ttAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGc                                                                                             <  1:1188401/150‑1 (MQ=255)
                                                         aaTCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCAt                                                                                           >  1:1002258/1‑150 (MQ=255)
                                                            cATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGt                                                                                        >  1:743828/1‑150 (MQ=255)
                                                            cATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGt                                                                                        <  2:1185748/150‑1 (MQ=255)
                                                             aTAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTa                                                                                       >  2:1178671/1‑150 (MQ=255)
                                                                 tGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGa                                                                                   >  1:1297121/1‑150 (MQ=255)
                                                                  gTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAg                                                                                  >  2:852165/1‑150 (MQ=255)
                                                                  gTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAg                                                                                  >  1:1242484/1‑150 (MQ=255)
                                                                    tgctgcCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCATGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGtt                                                                                >  1:346604/1‑150 (MQ=255)
                                                                          cTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCGCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCAt                                                                          <  1:1543439/150‑1 (MQ=255)
                                                                           ttGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATa                                                                         <  2:1537207/150‑1 (MQ=255)
                                                                             gCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGGCGCTGGTCGAGCATGGTACTGAGTTCGTCATACg                                                                       <  2:1120527/150‑1 (MQ=255)
                                                                              cGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGa                                                                      >  1:1210867/1‑150 (MQ=255)
                                                                                ggCTGTAACGATGGGCGTTTTATACAAGGTTACGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGTGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAgg                                                                    >  1:1308267/1‑150 (MQ=255)
                                                                                    gTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGgag                                                                >  1:1643511/1‑150 (MQ=255)
                                                                                      aaCGATGGGCGTTTTCTACCAGGTTTCGCAATAACATACGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGaa                                                              >  1:924667/1‑150 (MQ=255)
                                                                                        cGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATg                                                            >  1:682644/1‑150 (MQ=255)
                                                                                         gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATg                                                            >  1:440130/1‑149 (MQ=255)
                                                                                         gATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATg                                                            <  2:440130/149‑1 (MQ=255)
                                                                                              gCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCt                                                      >  2:428215/1‑150 (MQ=255)
                                                                                              gCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCt                                                      <  2:1434049/150‑1 (MQ=255)
                                                                                                gTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCGCATGCAATAGCAGGGTTTGCTGTCGCTTGTCGAGCATGGGACTGAGTTCGTCATACGAGGGGAGAATGACATCTTc                                                    <  2:260959/150‑1 (MQ=255)
                                                                                                     cTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                               >  2:911342/1‑150 (MQ=255)
                                                                                                     cTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCcagca                                               <  1:365698/150‑1 (MQ=255)
                                                                                                        gcAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACACATAACTGTGATGTCGGCTGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGATCATGGTACTCAGTTCGTCATACGAGCTGAGAATGACATATTCcagaaatt                                            >  1:238491/2‑145 (MQ=255)
                                                                                                        ccAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCAGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGCCATCTTCCAGCAGtt                                            >  1:238489/1‑150 (MQ=255)
                                                                                                         cAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGATTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGttt                                           >  1:633671/1‑150 (MQ=255)
                                                                                                          ccgcgtCGCAATAACATCCCCAGCAGGGTGGCATCACCCTGAACGGTGATGTCGGCGGCGCGCTCTGCCCATAGCAGGGTTTGCTGTCGCTGGGCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                          <  2:1648059/145‑1 (MQ=255)
                                                                                                          aGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                          <  1:1227315/150‑1 (MQ=255)
                                                                                                          aGGGTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGtttt                                          <  1:1048889/150‑1 (MQ=255)
                                                                                                                cGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGt                                    <  1:404142/150‑1 (MQ=255)
                                                                                                                 gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                   >  1:1410900/1‑150 (MQ=255)
                                                                                                                 gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                   >  1:647324/1‑150 (MQ=255)
                                                                                                                 gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                   <  2:1514127/150‑1 (MQ=255)
                                                                                                                 gCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                   >  1:966769/1‑150 (MQ=255)
                                                                                                                 gCAATAACATCCGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCGGTCGCTGGTCGAGCATGGTACTGAGCTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGtt                                   <  2:1048076/150‑1 (MQ=255)
                                                                                                                  cAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTg                                  >  1:519627/1‑150 (MQ=255)
                                                                                                                     tAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATa                               >  2:32372/1‑150 (MQ=255)
                                                                                                                     tAACATCCGCAGCACGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATa                               <  2:25264/150‑1 (MQ=255)
                                                                                                                      aaCATCCTCAGCAGGGTTGCATCACGCTGAACGTTGATGTCGGCGGCGCTCTCTGGCTATAGCAGGGTTTGCTGTCTCTGGTCGAGCATGGTACTGAGTTCGTCATACGATGGGAGAATGTCATCTTCCAGCATTTTTACATGTTGATaa                              <  1:588831/150‑1 (MQ=255)
                                                                                                                      aaCATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATaa                              >  1:1062972/1‑150 (MQ=255)
                                                                                                                         aTCCGCCGCAGGGGTGCATCTCCCTGAACGGTGATGTCGGCGGCGCTCTCTGTCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACAGAGTTCGTCATACGAGGGGAGAAAGACAGCTTCCAGCAGTTTTACATGTTGATAAttc                           <  1:1622220/150‑2 (MQ=255)
                                                                                                                         aTCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGGTTTACATGTTGATAtttt                           >  2:1089696/1‑149 (MQ=255)
                                                                                                                           ccGCAGCAGGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTAcc                         <  1:1697632/150‑1 (MQ=255)
                                                                                                                              cagcagGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGga                      >  1:198355/1‑150 (MQ=255)
                                                                                                                              cagcagGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGGCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTGTTACATGTTGATAATTACCGga                      >  2:534665/1‑150 (MQ=255)
                                                                                                                                 cagGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaaga                   >  1:789321/1‑150 (MQ=255)
                                                                                                                                 cagGGGTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaaga                   <  2:1712026/150‑1 (MQ=255)
                                                                                                                                  ccgccgacATCACCGTTCAGGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaagaa                  >  2:1669213/8‑150 (MQ=255)
                                                                                                                                   gggTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaa                 >  1:1501133/1‑150 (MQ=255)
                                                                                                                                   gggTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGaaa                 >  1:1280804/1‑150 (MQ=255)
                                                                                                                                     gTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaag                    <  2:794780/145‑1 (MQ=255)
                                                                                                                                     gTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGgaag                    >  1:794780/1‑145 (MQ=255)
                                                                                                                                     ccgacATCACCGTTCAGGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAAt               >  1:1654442/5‑150 (MQ=255)
                                                                                                                                       tGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa             >  2:811967/1‑150 (MQ=255)
                                                                                                                                       tGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTAGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGa             >  1:995676/1‑150 (MQ=255)
                                                                                                                                          aTCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTg          >  2:1513880/1‑150 (MQ=255)
                                                                                                                                           tCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGt         <  1:445611/150‑1 (MQ=255)
                                                                                                                                             aCCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTcc       >  1:1381737/1‑150 (MQ=255)
                                                                                                                                              cccTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCg      >  1:1581393/1‑150 (MQ=255)
                                                                                                                                                  gAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCAc  >  2:1334679/1‑150 (MQ=255)
                                                                                                                                                  gAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGGCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCAc  <  1:522717/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AATACCTGGTCCTTCATCTTCAACTGCCATGACCGCTCCGTCATCTTCTTGCAGCTTAATCATAATGTTGCTGCCTTGCGGGCTGTAACGATGGGCGTTTTCTACCAGGTTTCGCAATAACATCCGCAGCAGGGTTGCATCACCCTGAACGGTGATGTCGGCGGCGCTCTCTGGCAATAGCAGGGTTTGCTGTCGCTGGTCGAGCATGGTACTGAGTTCGTCATACGAGGGGAGAATGACATCTTCCAGCAGTTTTACATGTTGATAATTACCGGAAGAAAATGACTGTCCGGCAC  >  NC_000913/4332385‑4332680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: