Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,335,654 T→A 6.1% intergenic (‑64/+40) eptA ← / ← adiC lipid A phosphoethanolamine transferase/arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,335,6540TA 6.1% 371.0 / 9.4 131intergenic (‑64/+40)eptA/adiClipid A phosphoethanolamine transferase/arginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base T (48/75);  new base A (5/3);  total (53/78)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

Only 100 of 131 total aligned reads displayed.

CAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATA  >  NC_000913/4335507‑4335802
                                                                                                                                                   |                                                                                                                                                    
cAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTc                                                                                                                                                    >  2:57870/1‑150 (MQ=255)
  ggCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCtt                                                                                                                                                  >  1:533652/1‑150 (MQ=255)
  ggCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCtt                                                                                                                                                  >  1:1074723/1‑150 (MQ=255)
  ggCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCtt                                                                                                                                                  >  2:1237727/1‑150 (MQ=255)
   gCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTa                                                                                                                                                 <  1:1186099/150‑1 (MQ=255)
     aGATAGAGATATAAAAAGCGGCCAACGATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAg                                                                                                                                               >  1:1514097/1‑150 (MQ=255)
     aGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAg                                                                                                                                               <  2:1054629/150‑1 (MQ=255)
       atatAGATATATAAAGCGGCCAAACATATCCAGTCGAGTAATTTATAAGAGTTTGTTTTTAGTAGTCGCTTCAACATCTCGGTGTTTCGATCGAACAAATTGCGCAGATGGTCTAATAGCAAAATTAAGCCAACCTTAAGAACTTAATGt                                                                                                                                             <  2:656075/150‑1 (MQ=255)
        tAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGtt                                                                                                                                            >  2:1646145/1‑150 (MQ=255)
         agagATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGATGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTg                                                                                                                                           <  1:469055/150‑1 (MQ=255)
          gagaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTgg                                                                                                                                          >  1:325392/1‑150 (MQ=255)
          gagaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTATCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGAACTTAAGGTTgg                                                                                                                                          <  1:66576/150‑1 (MQ=255)
          gagaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGGTTCCATCGCACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGACAACCTAAAGTTAGTAAGGTTgg                                                                                                                                          >  1:266889/1‑150 (MQ=255)
          gagaTATAAAAAGCGGCCAACAAGAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTATTAGCTTCAACATCACTGTGATTCCATCGAACAAAGTTCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTgg                                                                                                                                          <  1:438303/150‑1 (MQ=255)
           agaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGTGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGc                                                                                                                                         <  1:1045261/150‑1 (MQ=255)
           agaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGGCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTgga                                                                                                                                         <  1:1045263/150‑2 (MQ=255)
            gaTATAAAAAGCGTCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCt                                                                                                                                        <  2:1436889/150‑1 (MQ=255)
            gaTATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCt                                                                                                                                        >  1:632310/1‑150 (MQ=255)
             atatAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCtt                                                                                                                                       >  2:1493266/1‑150 (MQ=255)
              tataAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCtta                                                                                                                                      <  2:1270158/150‑2 (MQ=255)
               ataAAAAGCGGCCAACAATAGCCAGTCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTTTTTCCATCGAACAAAGGGCGCATATGCTCGCAAAGCAAAATTAATCCAACCTTAAGTTCTTAAGGTTGGCtttt                                                                                                                                     <  2:52021/150‑1 (MQ=255)
                 aaaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGTGCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTAt                                                                                                                                   <  1:884672/150‑1 (MQ=255)
                 aaaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTAt                                                                                                                                   <  1:978376/150‑1 (MQ=255)
                  aaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTgg                                                                                                                                          <  2:140138/142‑1 (MQ=255)
                  aaaaGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTgg                                                                                                                                          >  1:140138/1‑142 (MQ=255)
                      gCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTg                                                                                                                              <  1:147827/150‑1 (MQ=255)
                      ccGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTg                                                                                                                              <  2:70958/149‑1 (MQ=255)
                        ggCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCt                                                                                                                            <  2:819030/150‑1 (MQ=255)
                           caacaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGa                                                                                                                         >  1:33002/1‑150 (MQ=255)
                             acaaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGAtt                                                                                                                       <  2:761337/150‑1 (MQ=255)
                              caaTAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTa                                                                                                                      <  1:694678/150‑1 (MQ=255)
                                 tAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAgg                                                                                                                   <  1:1422673/150‑1 (MQ=255)
                                  aGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAggg                                                                                                                  <  2:233197/150‑1 (MQ=255)
                                      aGGCGAGTAAATTCAAAGAGGGGCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAAt                                                                                                              <  1:344237/150‑1 (MQ=255)
                                            gTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCt                                                                                                        <  1:1237727/150‑1 (MQ=255)
                                             tAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGTAATTAATCtt                                                                                                       <  2:566261/150‑1 (MQ=255)
                                              aaaTTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCttt                                                                                                      >  2:1720706/1‑150 (MQ=255)
                                               aaTTCAAAGAGGTTCTTTTTAGTAGGCGCTTCATCATCACGGTGTTTCCATCTAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTg                                                                                                     <  1:1269762/150‑1 (MQ=255)
                                                 ttCAAAGAGGGTCTTTTTAGTAGTCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGGAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCt                                                                                                   <  2:891752/150‑1 (MQ=255)
                                                 ttCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACTTCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAACTTAAGCCAAGCTTATGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCt                                                                                                   <  1:1017839/150‑1 (MQ=255)
                                                     aaGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTAtt                                                                                               <  1:1493266/150‑1 (MQ=255)
                                                     aaGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTAtt                                                                                               >  2:68069/1‑150 (MQ=255)
                                                     aaGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTATATGTTTGCTGGATTAAGGCGAATTAATCTTTGCTTAtt                                                                                               >  1:1328337/1‑150 (MQ=255)
                                                      agagGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTg                                                                                              <  2:1544218/150‑1 (MQ=255)
                                                      agagGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTg                                                                                              <  2:1198730/150‑1 (MQ=255)
                                                       gagGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTgg                                                                                             <  1:291739/150‑1 (MQ=255)
                                                         gggTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTACGCCAACCTTAAGTTCTAAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTg                                                                                           <  1:1084406/150‑1 (MQ=255)
                                                         gggTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTg                                                                                           <  2:124831/150‑1 (MQ=255)
                                                         gggTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTg                                                                                           <  2:1544250/150‑1 (MQ=255)
                                                          ggTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGc                                                                                          >  2:927073/1‑150 (MQ=255)
                                                           ggcTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCa                                                                                         <  2:1094143/148‑1 (MQ=255)
                                                              tttttAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCt                                                                                      <  2:463254/150‑1 (MQ=255)
                                                                  tagtagGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAgg                                                                                  >  1:799642/1‑150 (MQ=255)
                                                                  tagtagGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAgg                                                                                  <  2:362923/150‑1 (MQ=255)
                                                                     tagGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCATAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGAt                                                                               <  1:797781/150‑1 (MQ=255)
                                                                      tggCGCTTCAACTTCTCGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGTTTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATa                                                                              <  2:1435172/149‑1 (MQ=255)
                                                                      agGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATa                                                                              <  2:1074723/150‑1 (MQ=255)
                                                                       ggCGCTTCAACATCACGGTGTTTCCATCGATCAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGTATTAAGGGGAATTAATCTTTGCTAATTGTTGCATCTATGGTATAc                                                                             >  2:897949/1‑150 (MQ=255)
                                                                          gcTTCAACATCAGGGTGTTTCCATCTAAAAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGTTTGGCTTTTATGTTTGATTTATTAAGGGTAATAAATCTTTGATTATTGGTCCATCTAAGGGATACggg                                                                          <  2:1467567/150‑1 (MQ=255)
                                                                              cAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGtttt                                                                      <  1:1655698/150‑1 (MQ=255)
                                                                                aCATCACGGTGTTTCCATCGCACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTa                                                                    <  2:63968/150‑1 (MQ=255)
                                                                                 cATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTAt                                                                   <  2:533652/150‑1 (MQ=255)
                                                                                 cATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTAt                                                                   <  2:1260360/150‑1 (MQ=255)
                                                                                  aTCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATg                                                                  <  1:171138/150‑1 (MQ=255)
                                                                                    cACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTa                                                                <  1:376016/150‑1 (MQ=255)
                                                                                     ccGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAg                                                               <  2:1709295/149‑1 (MQ=255)
                                                                                       ggTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGcc                                                             >  1:161616/1‑150 (MQ=255)
                                                                                       ggTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGcc                                                             <  1:1443124/150‑1 (MQ=255)
                                                                                          gtTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGt                                                          >  1:1144800/1‑150 (MQ=255)
                                                                                            ttCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTg                                                        <  2:1719575/150‑1 (MQ=255)
                                                                                              ccATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGTGTTTGTATGTAGCCGGTTGTa                                                      >  2:591821/1‑150 (MQ=255)
                                                                                              ccATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTa                                                      <  1:536540/150‑1 (MQ=255)
                                                                                               cATCGAACAAATTGAGGATATGCTCTCAAATCAAAATTATTCTAACCATAAATTCTTACGGTTGGCTTTTATGTTTTCTGGATTAAGGTGAATTAATCTTTGCTTTTTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTaa                                                     <  2:270204/150‑1 (MQ=255)
                                                                                                aTCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAAt                                                    <  2:519804/150‑1 (MQ=255)
                                                                                                   gAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCa                                                 >  2:973678/1‑150 (MQ=255)
                                                                                                         aGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCAt                                           >  1:1662376/1‑150 (MQ=255)
                                                                                                                       cGCAAAGCATAATTAAGCCATCCTTAAGTTCTTAAGGTTGGATTTTATGTTTGATGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTGTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatg                             <  2:1328337/150‑1 (MQ=255)
                                                                                                                       cGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGTGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatg                             <  2:325392/150‑1 (MQ=255)
                                                                                                                        gcaaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatga                            >  2:747423/1‑150 (MQ=255)
                                                                                                                        gcaaagcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGtgatga                            >  2:1539086/1‑150 (MQ=255)
                                                                                                                            agcaaaATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCaa                        >  2:1103903/1‑149 (MQ=255)
                                                                                                                               aaaaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAg                     <  1:124956/150‑1 (MQ=255)
                                                                                                                               aaaaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAg                     >  1:890592/1‑150 (MQ=255)
                                                                                                                                 aaTTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAggg                   <  1:965762/150‑1 (MQ=255)
                                                                                                                                      aGCCAACCTTAAGTTCTTAAGGGTGGCTTTTATCTTTGCTGGATTAAGGGGAATTAATCTTAGCTTATTGGTGCATCTAAGGGATACGGCTTTTTATGTAGCAGGTTTTAAATCATCGCATACATTGCGGTGATGAACATCAGGGTGACa              >  1:1058128/1‑150 (MQ=255)
                                                                                                                                       gCCAACCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACaa             >  2:674493/1‑150 (MQ=255)
                                                                                                                                        ccAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGTTTGTAATTCAGCTCATACATGGAGTTGATGACAATCAGGGTGACaaa            >  2:1462790/1‑150 (MQ=255)
                                                                                                                                        ccAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACaaa            >  1:534794/1‑150 (MQ=255)
                                                                                                                                         cAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAATGGATACGGGTTTTTATGTAGCCGGTTGTAATTCCGCGCATACAAGGCGGTGATGACCATCAGGGTGACAAAt           >  2:1286008/1‑150 (MQ=255)
                                                                                                                                         cAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAAt           >  2:1150269/1‑150 (MQ=255)
                                                                                                                                         cAACCTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAAt           >  2:457488/1‑150 (MQ=255)
                                                                                                                                          aaCCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATg          <  2:830422/150‑1 (MQ=255)
                                                                                                                                            ccTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAc        <  1:57870/150‑1 (MQ=255)
                                                                                                                                            ccTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAc        <  1:1720706/150‑1 (MQ=255)
                                                                                                                                            ccTTAAGAACTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGTTACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATAAATGGCGGTGATGACCATCAGGGTGACAAATGAc        >  2:403559/1‑150 (MQ=255)
                                                                                                                                             cTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAcc       <  2:161616/150‑1 (MQ=255)
                                                                                                                                             cTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGAcc       >  1:1159641/1‑150 (MQ=255)
                                                                                                                                             cTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGAATACATGGCGGTGATGACCATCAGGGTGACAAATGAcc       >  1:1093401/1‑150 (MQ=255)
                                                                                                                                               tAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACcac     <  2:1159641/150‑1 (MQ=255)
                                                                                                                                                  gTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAATGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATAAGGGTGACAAATGCCCACATa  <  2:1589946/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAGGCAGATAGAGATATAAAAAGCGGCCAACAATAGCCAGGCGAGTAAATTCAAAGAGGGTCTTTTTAGTAGGCGCTTCAACATCACGGTGTTTCCATCGAACAAAGTGCGCATATGCTCGCAAAGCAAAATTAAGCCAACCTTAAGTTCTTAAGGTTGGCTTTTATGTTTGCTGGATTAAGGGGAATTAATCTTTGCTTATTGGTGCATCTAAGGGATACGGGTTTTTATGTAGCCGGTTGTAATTCAGCGCATACATGGCGGTGATGACCATCAGGGTGACAAATGACCACATA  >  NC_000913/4335507‑4335802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: