Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 543,749 T→A 5.8% T100S (ACA→TCA) ‡ allE ← S‑ureidoglycine aminohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913543,7490TA 5.8% 361.9 / 8.7 120T100S (ACA→TCA) ‡allES‑ureidoglycine aminohydrolase
Reads supporting (aligned to +/- strand):  ref base T (50/63);  new base A (2/5);  total (52/68)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

Only 100 of 125 total aligned reads displayed.

GCCAGAAACCAGCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAG  >  NC_000913/543600‑543897
                                                                                                                                                     |                                                                                                                                                    
gCCAGAAACCAGCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGt                                                                                                                                                      >  1:716430/1‑150 (MQ=255)
 ccAGAAACCAGCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGtt                                                                                                                                                     >  1:1022167/1‑150 (MQ=255)
 ccAGAAACCAGCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGtt                                                                                                                                                     >  1:1694991/1‑150 (MQ=255)
  cAGAAACCAGCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGttt                                                                                                                                                    >  2:1437898/1‑150 (MQ=255)
    gAAACCAGCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCCCCTTCGCTTAAGGCAAATGTTTTg                                                                                                                                                  <  2:1000519/150‑1 (MQ=255)
     aaaCCAGCCACGGTGAATAGCCTTATACAGGAACATAGCGTCTCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGc                                                                                                                                                 <  2:324871/150‑1 (MQ=255)
       aCCAGCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCt                                                                                                                                               >  2:1250332/1‑150 (MQ=255)
        ccAGCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCtt                                                                                                                                              >  1:630665/1‑150 (MQ=255)
         cAGCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTc                                                                                                                                             >  2:755976/1‑150 (MQ=255)
           gCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAAACATTTGCCttaag                                                                                                                                           <  2:805093/150‑4 (MQ=255)
            ccACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAAACATTTGCCttaagc                                                                                                                                          <  1:473877/150‑5 (MQ=255)
            ccACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAAGAAAGATAGCCACCTTCGCTTAAGTCAAATGTTTTGCCTTCGGc                                                                                                                                          >  1:1202046/1‑150 (MQ=255)
            ccACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCGGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGACTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAAACATTTGCCttaagc                                                                                                                                          <  2:44104/150‑5 (MQ=255)
            ccACGGTGCATAGCCTTCTACCGGAACATAGCCGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCCGAAGGCAAAACATTTGCCttaagc                                                                                                                                          <  1:282259/150‑5 (MQ=255)
             cACGGTGCATAGCCTTCTCCCTGAACATAGCGGCGCTTATATAAAAAGATTTGTCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCt                                                                                                                                         <  1:876543/150‑1 (MQ=255)
              aCGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTTGGCGTTAACAAACGTCATTAAGGAGCCTGGCTGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAAACATTTGCCttaagcga                                                                                                                                        <  1:638829/150‑7 (MQ=255)
                ggTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTg                                                                                                                                      <  1:58173/150‑1 (MQ=255)
                  tGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGc                                                                                                                                    <  2:1286273/150‑1 (MQ=255)
                  tGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGc                                                                                                                                    <  1:565945/150‑1 (MQ=255)
                     aTAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTg                                                                                                                                >  2:846245/1‑150 (MQ=255)
                     aTAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGGCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGt                                                                                                                                 <  2:1000448/150‑1 (MQ=255)
                      tAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTg                                                                                                                                <  1:1617706/150‑1 (MQ=255)
                      tAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTg                                                                                                                                <  2:1547688/150‑1 (MQ=255)
                      tAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTg                                                                                                                                <  1:1732469/150‑1 (MQ=255)
                       aGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGa                                                                                                                               <  2:1620324/150‑1 (MQ=255)
                        gCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCgg                                                                                                                                           >  1:106504/1‑137 (MQ=255)
                        gCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCgg                                                                                                                                           <  2:106504/137‑1 (MQ=255)
                        gCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGat                                                                                                                              <  2:1558326/150‑1 (MQ=255)
                              tACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTcc                                                                                                                        <  2:429917/150‑1 (MQ=255)
                              tACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCAACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTcc                                                                                                                        >  2:277318/1‑150 (MQ=255)
                               aCCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCa                                                                                                                       >  1:79946/1‑150 (MQ=255)
                                ccGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCag                                                                                                                      <  2:1400805/150‑1 (MQ=255)
                                ccGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCag                                                                                                                      <  2:1717846/150‑1 (MQ=255)
                                ccGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCag                                                                                                                      <  2:1711259/150‑1 (MQ=255)
                                  ggAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCagag                                                                                                                    >  1:82218/1‑150 (MQ=255)
                                     aCATAGCGGAGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTTGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGAAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATc                                                                                                                 <  2:536222/150‑1 (MQ=255)
                                      cATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTACGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATca                                                                                                                >  1:1322635/1‑150 (MQ=255)
                                      cATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTATTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATca                                                                                                                <  1:417008/150‑1 (MQ=255)
                                      cATAGCGGCGCTTATATAAAAAGATTTGACTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATca                                                                                                                <  1:429431/150‑1 (MQ=255)
                                         aGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACAt                                                                                                             <  2:357960/150‑1 (MQ=255)
                                         aGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACAt                                                                                                             >  1:151190/1‑150 (MQ=255)
                                         aGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACAt                                                                                                             >  2:156085/1‑150 (MQ=255)
                                          gcggcgCTTATATATAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATa                                                                                                            <  1:324585/150‑1 (MQ=255)
                                           cggcgCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATAc                                                                                                           <  1:364982/150‑1 (MQ=255)
                                                cTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGaa                                                                                                      >  2:1438264/1‑150 (MQ=255)
                                                  tatataAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCATTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACg                                                                                                    >  1:1681437/1‑150 (MQ=255)
                                                     ataAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGttt                                                                                                 <  2:207774/150‑1 (MQ=255)
                                                      taAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTTAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTc                                                                                                <  1:1016796/150‑1 (MQ=255)
                                                       aaaaaGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCa                                                                                               >  1:1503829/1‑150 (MQ=255)
                                                       aaaaaGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCa                                                                                               >  1:983797/1‑150 (MQ=255)
                                                       aaaaaGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCa                                                                                               >  2:1231109/1‑150 (MQ=255)
                                                         aaaGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCtt                                                                                                                                        >  1:760785/1‑107 (MQ=255)
                                                         aaaGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCtt                                                                                                                                        <  2:760785/107‑1 (MQ=255)
                                                         aaaGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAAt                                                                                             >  2:1267096/1‑150 (MQ=255)
                                                                 ggCTGTCTTCGGCCTGGGCGTTAACAAACGTCATAAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGTCAGTGATATTTCCAGAGATCACATACAGGAACGTATCAATGCCTTCGc                                                                                     >  1:1439876/1‑150 (MQ=255)
                                                                   cTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCa                                                                                   <  1:1186942/150‑1 (MQ=255)
                                                                     gTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCAcc                                                                                 <  2:502428/150‑1 (MQ=255)
                                                                      gcTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCg                                                                                <  2:1694991/149‑1 (MQ=255)
                                                                         tCGGCATGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATATCCTCCTTCGCTTAAGGCAAATTTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGTAACGTTTCAATGCCTTCGCCACCGAAg                                                                             <  2:856531/150‑1 (MQ=255)
                                                                          cGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGc                                                                            >  2:1018474/1‑150 (MQ=255)
                                                                           ggCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGcc                                                                           <  1:1588700/150‑1 (MQ=255)
                                                                            gCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGccc                                                                          <  1:1382277/150‑1 (MQ=255)
                                                                                gggCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTgtt                                                                      >  2:1181621/1‑150 (MQ=255)
                                                                                gggCGTTAACAAACGTCATTAAGGAGCCTGGCGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTCCGCCACCGAAGCCCTgttg                                                                     <  1:846245/150‑1 (MQ=255)
                                                                                  gCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTgttgg                                                                    >  1:32430/1‑150 (MQ=255)
                                                                                   cGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTgttggt                                                                   <  2:79946/150‑1 (MQ=255)
                                                                                    gTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTgttggtt                                                                  <  2:32430/150‑1 (MQ=255)
                                                                                    gTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCAACCGAAGCCCTgttggtt                                                                  >  2:995052/1‑150 (MQ=255)
                                                                                       aaCAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGcc                                                               >  2:1142010/1‑150 (MQ=255)
                                                                                        aCAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCa                                                              >  1:659577/1‑150 (MQ=255)
                                                                                          aaaCGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCAcc                                                            <  1:1213422/150‑1 (MQ=255)
                                                                                               tCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGtttt                                                       >  1:833035/1‑150 (MQ=255)
                                                                                                cATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTg                                                      >  2:402037/1‑150 (MQ=255)
                                                                                                 aTTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGa                                                     >  2:1182193/1‑150 (MQ=255)
                                                                                                 aTTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGa                                                     >  1:707743/1‑150 (MQ=255)
                                                                                                  ttAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGAt                                                    <  1:1437898/150‑1 (MQ=255)
                                                                                                    aaGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGc                                                  <  1:575025/150‑1 (MQ=255)
                                                                                                    aaGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGc                                                  <  2:716430/150‑1 (MQ=255)
                                                                                                        aGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAgtg                                              <  2:1480782/150‑1 (MQ=255)
                                                                                                         gCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGACGCCCTGTTGGTTGCCAGCGGTTTGATGCAgtgt                                             >  2:981268/1‑150 (MQ=255)
                                                                                                                gggCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGa                                      <  1:277318/150‑1 (MQ=255)
                                                                                                                   cAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATaa                                   <  1:1250332/150‑1 (MQ=255)
                                                                                                                   cAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTAGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCGACCGTTTTGATCAAGTTAGACCAGATaa                                   >  2:1083249/1‑150 (MQ=255)
                                                                                                                      tAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATca                                <  1:755976/150‑1 (MQ=255)
                                                                                                                        aaGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATcaac                              >  1:692240/1‑150 (MQ=255)
                                                                                                                         aGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACATGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATcaaca                             <  2:1317265/150‑1 (MQ=255)
                                                                                                                                   cTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCAc                   >  1:572453/1‑150 (MQ=255)
                                                                                                                                    ttCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCTATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCAcc                  <  1:1075021/150‑1 (MQ=255)
                                                                                                                                        ccgaaGGCAAAACATTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGc              >  2:50013/4‑150 (MQ=255)
                                                                                                                                        ccgaaGGCAAAACATTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTGATGGAGTGGGGCCATCTAAAAAACAAAACCTGCACCCCGca             >  1:1392015/4‑149 (MQ=255)
                                                                                                                                          tAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGCGGCACCCAGttt            >  1:1264504/1‑150 (MQ=255)
                                                                                                                                           aaGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGCGATCCCATCCAGGAACGTTTCAATGCCTGCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCttt           <  2:284720/150‑1 (MQ=255)
                                                                                                                                            aGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAAGCAACAAAAGAGGCACCCAGCTTTg          >  2:1006270/1‑150 (MQ=255)
                                                                                                                                              gCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGc        >  1:1389422/1‑150 (MQ=255)
                                                                                                                                              gCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATAACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATATTCTACAAAAGAGGCACCCAGCTTTGGc        >  1:1215343/1‑150 (MQ=255)
                                                                                                                                                aaaTGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACACAGCTTTGGCGt      >  1:1355138/1‑150 (MQ=255)
                                                                                                                                                 aaTGTTTTTCCTTCGGCTTTGTCAGTTATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCTCCTAAGCCCTGTTGGTTGCCTCCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTg     <  1:155991/150‑1 (MQ=255)
                                                                                                                                                    tttttGCCTTCGTCTTTGGCAGTGATATTTCCAGAGATCACATTCAGGAACGTTTCAATGCCTTCGCCACAGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGAACAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGgag  <  1:1560822/149‑1 (MQ=255)
                                                                                                                                                    gTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGCAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGAGCAGATAATCACCTAAAGCGGCACCCCGCTTTGGCGTGtag  >  1:282222/1‑150 (MQ=255)
                                                                                                                                                    gTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGgag  >  1:1342156/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCAGAAACCAGCCACGGTGCATAGCCTTCTACCGGAACATAGCGGCGCTTATATAAAAAGATTTGGCTGTCTTCGGCCTGGGCGTTAACAAACGTCATTAAGGAGCCTGGCGGGCAATAAAGATAGCCACCTTCGCTTAAGGCAAATGTTTTGCCTTCGGCTTTGGCAGTGATATTTCCAGAGATCACATACAGGAACGTTTCAATGCCTTCGCCACCGAAGCCCTGTTGGTTGCCACCGTTTTGATGCAGTGTGACCAGATAATCAACAAAAGAGGCACCCAGCTTTGGCGTGGAG  >  NC_000913/543600‑543897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: