Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,353,171 C→G 6.3% intergenic (+90/+29) ghoT → / ← lysU toxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,353,1710CG 6.3% 556.2 / 18.9 172intergenic (+90/+29)ghoT/lysUtoxin of GhoTS toxin‑antitoxin pair; membrane‑lytic protein; stimulator of persister cell formation/lysine tRNA synthetase, inducible
Reads supporting (aligned to +/- strand):  ref base C (78/83);  new base G (6/5);  total (84/88)
Fisher's exact test for biased strand distribution p-value = 7.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 175 total aligned reads displayed.

CTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATAT  >  NC_000913/4353022‑4353320
                                                                                                                                                     |                                                                                                                                                     
cTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGc                                                                                                                                                       >  1:1188600/1‑150 (MQ=255)
 tGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGcc                                                                                                                                                      <  1:1710982/150‑1 (MQ=255)
  ggTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCt                                                                                                                                                     >  2:1217078/1‑150 (MQ=255)
   gTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTg                                                                                                                                                    <  2:815566/150‑1 (MQ=255)
     cGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGTAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGAc                                                                                                                                                  >  1:289147/1‑150 (MQ=255)
      ggAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACt                                                                                                                                                 >  2:1039257/1‑150 (MQ=255)
         aTAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCt                                                                                                                                              <  1:516332/150‑1 (MQ=255)
          tAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCtt                                                                                                                                             >  2:147295/1‑150 (MQ=255)
           aaCCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTc                                                                                                                                            <  1:605498/150‑1 (MQ=255)
            aCCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCg                                                                                                                                           <  1:1621374/150‑1 (MQ=255)
            aCCTGGCCAATGAGGCTGCCTGGGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCAGGCGACTGCTTCg                                                                                                                                           <  1:1515420/150‑1 (MQ=255)
             ccTGGCCAATGAGTCTGCCTGTGTCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGCGGACCCGCTGGTTATCGTGAAACGAAGCGCCTCTCCTCAAAACTGGATGTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGt                                                                                                                                          <  1:131791/150‑1 (MQ=255)
             ccTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGCCCCGCTGGTTATCGTGAAACGAAGCGGCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGGCGCCTGACTGCTGCGt                                                                                                                                          <  2:954768/150‑1 (MQ=255)
             ccTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGt                                                                                                                                          <  1:1595371/150‑1 (MQ=255)
             ccTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCAGGCGACTGCTTCGt                                                                                                                                          <  1:1147702/150‑1 (MQ=255)
                ggCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCa                                                                                                                                       <  2:1182543/150‑1 (MQ=255)
                 gCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCAt                                                                                                                                      >  2:945506/1‑150 (MQ=255)
                  ccAATGAGTCTGCCTGTGGTATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCAtt                                                                                                                                     >  1:941800/1‑150 (MQ=255)
                   cAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTa                                                                                                                                    <  2:810423/150‑1 (MQ=255)
                   cAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAAAGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTAATTa                                                                                                                                    >  2:373833/1‑150 (MQ=255)
                         gTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGa                                                                                                                              >  2:852971/1‑150 (MQ=255)
                         gTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGa                                                                                                                              >  2:158170/1‑150 (MQ=255)
                            tGCGTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCATGACTGCTTCGTTCATTAAAGTGAAat                                                                                                                           <  2:97650/150‑1 (MQ=255)
                            tGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAat                                                                                                                           <  1:1467684/150‑1 (MQ=255)
                            tGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAat                                                                                                                           <  1:1412549/150‑1 (MQ=255)
                               cTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAattta                                                                                                                        >  2:639900/1‑150 (MQ=255)
                                 gtgGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAatttatt                                                                                                                      <  2:132776/150‑1 (MQ=255)
                                   ggCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTc                                                                                                                    >  2:779630/1‑150 (MQ=255)
                                   ggCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTc                                                                                                                    >  2:886559/1‑150 (MQ=255)
                                    gCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCt                                                                                                                   >  2:1630586/1‑150 (MQ=255)
                                      aTTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCTTTCATTAAAGTGAAATTTATTTCtgt                                                                                                                 >  2:90590/1‑150 (MQ=255)
                                                tctctcTTTTAGGAGCTGTAGTTGAGTGGACCCGCAGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTCCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCg                                                                                                       >  1:1504834/1‑150 (MQ=255)
                                                    tctTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCgg                                                                                                   <  2:298744/150‑1 (MQ=255)
                                                     ctTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCggg                                                                                                  >  1:449830/1‑150 (MQ=255)
                                                      ttttAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGa                                                                                                 <  2:478551/150‑1 (MQ=255)
                                                        ttAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAag                                                                                               >  2:920554/1‑150 (MQ=255)
                                                          tggAGCTGTAGTGGAGTGGACCCGCTGGTTATCGTGAAACGAAGGGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAagag                                                                                             <  2:1519000/149‑1 (MQ=255)
                                                          aGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAagag                                                                                             <  2:550798/150‑1 (MQ=255)
                                                            gAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGaa                                                                                           <  2:500822/150‑1 (MQ=255)
                                                             aGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCAGGAAGAGAAt                                                                                          <  2:332431/150‑1 (MQ=255)
                                                             aGCTGTAGTTGAGTGGAACCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAAt                                                                                          <  2:1274153/150‑1 (MQ=255)
                                                              gCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATa                                                                                         <  1:912626/150‑1 (MQ=255)
                                                                tGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAAc                                                                                       <  1:972132/150‑1 (MQ=255)
                                                                 gTAGTTGAGGGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGGCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACg                                                                                      <  2:1164528/150‑1 (MQ=255)
                                                                   aGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTc                                                                                    >  2:1293089/1‑150 (MQ=255)
                                                                    gTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTcg                                                                                   >  1:1043568/1‑150 (MQ=255)
                                                                      tGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTcgcg                                                                                 <  2:422224/150‑1 (MQ=255)
                                                                      tGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTcgcg                                                                                 <  1:779630/150‑1 (MQ=255)
                                                                      tGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTAATTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTcgcg                                                                                 >  2:1424022/1‑150 (MQ=255)
                                                                        aGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGaa                                                                               >  2:1729451/1‑150 (MQ=255)
                                                                           ggACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATat                                                                            >  2:797199/1‑149 (MQ=255)
                                                                              cccGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTAt                                                                         >  2:155186/1‑150 (MQ=255)
                                                                               ccGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATg                                                                        <  2:659323/150‑1 (MQ=255)
                                                                                cGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATgg                                                                       <  2:519779/150‑1 (MQ=255)
                                                                                 gCTGGTTATCGTTAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGATATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGc                                                                      <  2:515959/150‑1 (MQ=255)
                                                                                 gCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGc                                                                      <  1:4667/150‑1 (MQ=255)
                                                                                       tATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAg                                                                >  1:674415/1‑150 (MQ=255)
                                                                                       tATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAg                                                                <  1:639900/150‑1 (MQ=255)
                                                                                        aTCGTGAAACGAAGCGTCTCTCCTCAAATCTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTGCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCGGTTAGt                                                               >  2:202058/1‑150 (MQ=255)
                                                                                          cGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaa                                                             >  1:1732296/1‑150 (MQ=255)
                                                                                          cGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaa                                                             <  1:630655/150‑1 (MQ=255)
                                                                                          cGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTATTAGTaa                                                             >  1:189670/1‑150 (MQ=255)
                                                                                           gTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaaa                                                            >  2:196575/1‑150 (MQ=255)
                                                                                            tGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATAAAAGTTAAATTAATTTCTGTGGACCCAACGCCGGGAAAAAAAAACCGACCCAAATATTATGGCTGTAAGAAAAc                                                           >  2:609955/1‑150 (MQ=255)
                                                                                                 cGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCAt                                                      <  2:419791/150‑1 (MQ=255)
                                                                                                   aaGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGATAGTAAACAGCATaa                                                    >  1:1069734/1‑150 (MQ=255)
                                                                                                     gCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATc                                                  >  1:117999/1‑150 (MQ=255)
                                                                                                      cGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCa                                                 <  1:1293089/150‑1 (MQ=255)
                                                                                                       gTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCAt                                                >  2:510098/1‑150 (MQ=255)
                                                                                                        tctctcCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCTCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCAtt                                               >  2:1473254/1‑150 (MQ=255)
                                                                                                          tctcCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCg                                             <  1:242581/150‑1 (MQ=255)
                                                                                                           ctcctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCgg                                            <  2:596515/150‑1 (MQ=255)
                                                                                                           ctcctcAAAACTGGATTTGCGCAACGATGAACGAAGCAGTCGCCTGACTGATTCGTTCAATAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGATAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCAGTCgg                                            >  2:866399/1‑150 (MQ=255)
                                                                                                             cctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGTAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTc                                          <  2:1095978/150‑1 (MQ=255)
                                                                                                             cctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGAATAATCATTCGGTc                                          >  1:726851/1‑150 (MQ=255)
                                                                                                              ctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCg                                         <  1:323370/150‑1 (MQ=255)
                                                                                                              ctcAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCg                                         >  1:717495/1‑150 (MQ=255)
                                                                                                                cAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGAt                                       <  1:1729451/150‑1 (MQ=255)
                                                                                                                   aaCTGGTTTTGCGGAACGATGAACGATGCAGTCTCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATGGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                    <  2:225113/150‑1 (MQ=255)
                                                                                                                   aaCTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                    <  2:1698131/150‑1 (MQ=255)
                                                                                                                   aaCTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGAATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc                                    <  2:1188600/150‑1 (MQ=255)
                                                                                                                       ggATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATa                                >  1:1374782/1‑150 (MQ=255)
                                                                                                                        gATTTGCGGAACGATGAACGAAGCAGTCTCCTGACTGCTTAGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAc                               <  2:289147/150‑1 (MQ=255)
                                                                                                                          tttGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAccc                             <  2:450120/150‑1 (MQ=255)
                                                                                                                            tGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAg                           >  2:1246307/1‑150 (MQ=255)
                                                                                                                               ggAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTATAGTGTAAGTTATTTCTGTGGGCGCAGCGCCGGGAAGAGAAGAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATCCCCAGAcc                        >  2:314334/1‑150 (MQ=255)
                                                                                                                               ggAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAcc                        <  1:770746/150‑1 (MQ=255)
                                                                                                                                  aCGATGAACGAAGCAGGCGCCTGACTGCTTAGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGc                     <  1:314475/150‑1 (MQ=255)
                                                                                                                                    gATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCgg                   >  1:300739/1‑150 (MQ=255)
                                                                                                                                    gATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCgg                   >  1:55474/1‑150 (MQ=255)
                                                                                                                                      tGAACGAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGCCCAGCGGtt                 >  1:1552745/1‑150 (MQ=255)
                                                                                                                                        aaCGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTgg               <  1:852971/150‑1 (MQ=255)
                                                                                                                                        aaCGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTgg               >  1:1664662/1‑150 (MQ=255)
                                                                                                                                           gAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggcgg            <  2:1259327/150‑1 (MQ=255)
                                                                                                                                           gAAGCAGTCGCCTGACTGCTTCGTTCATAAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACACAGACCAGCCGTTggcgg            >  1:1039083/1‑150 (MQ=255)
                                                                                                                                           gAAGCAGTCAGGCGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTggcgg            >  1:1368168/1‑150 (MQ=255)
                                                                                                                                            aaGCAGTCGCCTGACTGCTTAGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAAAAACGTCGCGAATAGTATGGCAGTTAGTAAACAGCATAATCATTCGGTCGATACCAAAACCCAGACAAGCGGTTggcggc           >  2:948463/1‑150 (MQ=255)
                                                                                                                                                 gTCGCTTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGAcc      >  2:439733/1‑150 (MQ=255)
                                                                                                                                                 gTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGAcc      <  1:1564817/150‑1 (MQ=255)
                                                                                                                                                     ccTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGATGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCatat  >  2:699636/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTTTTTCTCTCTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATAT  >  NC_000913/4353022‑4353320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: