Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,376,280 G→A 5.2% intergenic (+15/‑37) efp → / → ecnA polyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,376,2800GA 5.2% 425.4 / 5.1 132intergenic (+15/‑37)efp/ecnApolyproline‑specific translation elongation factor EF‑P/entericidin A membrane lipoprotein, antidote entericidin B
Reads supporting (aligned to +/- strand):  ref base G (66/59);  new base A (2/5);  total (68/64)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 136 total aligned reads displayed.

ATACCGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCG  >  NC_000913/4376132‑4376429
                                                                                                                                                    |                                                                                                                                                     
aTACCGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCgg                                                                                                                                                      <  1:1252741/150‑1 (MQ=255)
   ccGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCt                                                                                                                                                   >  2:755125/1‑150 (MQ=255)
    cGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTg                                                                                                                                                  >  2:1165423/1‑150 (MQ=255)
       aGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAg                                                                                                                                               <  1:385889/150‑1 (MQ=255)
        ggTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAgg                                                                                                                                              >  1:138708/1‑150 (MQ=255)
         gTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGc                                                                                                                                             <  1:321448/150‑1 (MQ=255)
            cTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTTGTTAAAGTTCATCTGTTTGTACAAATCGTCGAAGTCATCAAAGTGGATACCCGATCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTTGTGCGGCCTGCAGGCTGc                                                                                                                                          <  1:1501822/150‑1 (MQ=255)
            cTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGc                                                                                                                                          >  2:993625/1‑150 (MQ=255)
              ggtggCAAACCGGCTTCCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATTGGCGAAGACATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTTCGGCCTGCAGGCTGCAc                                                                                                                                        >  2:345678/1‑150 (MQ=255)
              ggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCAGCCTGCAGGCCGCAc                                                                                                                                        <  1:1009361/150‑1 (MQ=255)
              ggtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGGTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAc                                                                                                                                        <  1:319291/150‑1 (MQ=255)
               gtggCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAcc                                                                                                                                       <  1:680110/150‑1 (MQ=255)
               ggggCAAACCGGCTACCCTGTCTCCTGGCGCGGTGGGTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATCCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCAcc                                                                                                                                       <  2:1068579/148‑1 (MQ=255)
                   cAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCCCCATCa                                                                                                                                   >  2:513528/1‑150 (MQ=255)
                   cAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCa                                                                                                                                   >  2:696606/1‑150 (MQ=255)
                      cccGGCTACCCTGGCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAACTCGGCGAAGGCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACtt                                                                                                                                <  1:98753/149‑1 (MQ=255)
                      aCCGGCTACCCTGGCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGGGCAGCCTGCAGGCCGCACCacaaccg                                                                                                                                <  1:588642/150‑7 (MQ=255)
                       ccGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTa                                                                                                                               <  2:1585136/150‑1 (MQ=255)
                       ccGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTa                                                                                                                               <  2:279086/150‑1 (MQ=255)
                       ccGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTa                                                                                                                               >  2:415405/1‑150 (MQ=255)
                               ccTGTCTACTGGCGCTGGGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCGGCACCATCACTTATTCAGGTc                                                                                                                       <  1:484973/150‑1 (MQ=255)
                                   tCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCagag                                                                                                                   >  1:1447623/1‑150 (MQ=255)
                                      aCTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAgatg                                                                                                                >  1:91347/1‑150 (MQ=255)
                                                tgGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGAGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGcc                                                                                                      <  2:299313/150‑1 (MQ=255)
                                                 ggTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCACAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                                                                     <  1:1456310/150‑1 (MQ=255)
                                                 ggTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCt                                                                                                     >  2:199120/1‑150 (MQ=255)
                                                      aaGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCg                                                                                                >  2:1184185/1‑150 (MQ=255)
                                                      aaGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCg                                                                                                >  1:453787/1‑150 (MQ=255)
                                                        gTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGtt                                                                                              >  2:518136/1‑150 (MQ=255)
                                                          tCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCt                                                                                            >  2:723786/1‑150 (MQ=255)
                                                            cGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTg                                                                                          >  1:1473374/1‑150 (MQ=255)
                                                            cGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTg                                                                                          <  2:801217/150‑1 (MQ=255)
                                                             gCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGt                                                                                         <  1:86031/150‑1 (MQ=255)
                                                               tGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCGCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGttt                                                                                       <  1:921401/150‑1 (MQ=255)
                                                                gTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGtttt                                                                                      <  2:1706708/150‑1 (MQ=255)
                                                                  ttGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGATCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGGTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGATACGCCTTATCGTTCTTGTTttgc                                                                                    <  2:375880/150‑1 (MQ=255)
                                                                   tGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                                                                   >  2:1313014/1‑150 (MQ=255)
                                                                   tGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGGGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCCCCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgct                                                                                   >  1:1564627/1‑150 (MQ=255)
                                                                    gTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                                                                  >  1:1091357/1‑150 (MQ=255)
                                                                    gTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgctt                                                                                  >  1:896715/1‑150 (MQ=255)
                                                                     tACTAATCGGCGAAGTCATCATAGTGGATACCCGCGCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCGTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                                 <  2:832700/150‑1 (MQ=255)
                                                                     tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                                 <  2:541128/150‑1 (MQ=255)
                                                                     tACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGGGAATACGTCTCTCGCGGGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCGGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTttgcttg                                                                                 <  2:1362077/150‑1 (MQ=255)
                                                                      aCAAATCGGCGAAGTCATCAAAGTGGATCCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCCGGATCAGTTATTCTGGTCAGTGATGTTGAAGCGCCTGTTCGTTCTTGTTttgcttgc                                                                                >  2:921617/1‑150 (MQ=255)
                                                                       cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                               >  2:437217/1‑150 (MQ=255)
                                                                       cAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGcc                                                                               <  2:1692908/150‑1 (MQ=255)
                                                                        aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCa                                                                              >  1:1008876/1‑150 (MQ=255)
                                                                        aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCa                                                                              >  1:1143087/1‑150 (MQ=255)
                                                                        aaaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCGGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCa                                                                              >  2:1421432/1‑150 (MQ=255)
                                                                         aaTCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAg                                                                             >  1:845131/1‑150 (MQ=255)
                                                                               cGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgct                                                                       <  1:518136/150‑1 (MQ=255)
                                                                                 aaGTCATCAACGTGGATACCCGCTCTGGTGAATACGTGTCTGGCGTTAAGTAATGCGGTTGTGGTGCGGCCTGCAGGGTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCAGgctgc                                                                     <  1:1334689/150‑1 (MQ=255)
                                                                                  aGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACgctgct                                                                    >  1:1588839/1‑150 (MQ=255)
                                                                                   gTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTc                                                                   >  1:1108740/1‑150 (MQ=255)
                                                                                   gTCATCAAAGTGGATACCCGCTCTGGTGAATACGGCTCTCGCGTGAAGTAATGCGGTTGTGGGGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTc                                                                   <  1:1662288/150‑1 (MQ=255)
                                                                                     catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCCCTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTAGTTTTGCTTCCCAGCACGCTGCTCAc                                                                 >  2:1260181/1‑150 (MQ=255)
                                                                                     catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCAc                                                                 >  1:339080/1‑150 (MQ=255)
                                                                                     catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCAc                                                                 >  2:215502/1‑150 (MQ=255)
                                                                                     catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCAc                                                                 >  2:785096/1‑150 (MQ=255)
                                                                                     catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCAc                                                                 >  2:1175771/1‑150 (MQ=255)
                                                                                     catcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAAAGACTTATCGTTCTTGTTTTGCTTGCCAGCACGATGCTCAc                                                                 >  2:1540988/1‑150 (MQ=255)
                                                                                      atcaAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTAACg                                                                >  1:1234929/1‑150 (MQ=255)
                                                                                      atcaAAGGGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGGTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACg                                                                <  2:334327/150‑1 (MQ=255)
                                                                                         aaaGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGATGTAATGCGGTTGTGGTGCGGCCTGCTGGCTGCACCATCAGTTATTCAGGTCAGAGCTGATGCAACGCCTTTTCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGc                                                             >  2:255310/1‑150 (MQ=255)
                                                                                         aaaGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGc                                                             <  2:230803/150‑1 (MQ=255)
                                                                                           aGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTg                                                           <  2:1395254/150‑1 (MQ=255)
                                                                                            gTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGt                                                          <  2:1143087/150‑1 (MQ=255)
                                                                                            gTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGt                                                          <  2:505296/150‑1 (MQ=255)
                                                                                               gATACCCGCGCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAac                                                       <  2:1123318/150‑1 (MQ=255)
                                                                                                aTACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCCTCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCCCGCTGCTCACGGGCTGTAaca                                                      >  2:1256691/1‑150 (MQ=255)
                                                                                                aTACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAaca                                                      >  2:1024970/1‑150 (MQ=255)
                                                                                                  aCCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACAcc                                                    >  2:198794/1‑150 (MQ=255)
                                                                                                    ccGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGc                                                  >  2:1375802/1‑150 (MQ=255)
                                                                                                           ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTTTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCATAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGt                                           >  2:369549/1‑150 (MQ=255)
                                                                                                           ggTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGt                                           >  1:684726/1‑150 (MQ=255)
                                                                                                            gTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGGGGTGCGGCCTGCAGGCTGCACCAGCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTGTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGtt                                          <  2:1297527/150‑1 (MQ=255)
                                                                                                              gAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTTCCAGCCCGCTGCTCAAGGGCTGTAACACCGCTCGCGGtttg                                        >  1:875651/1‑149 (MQ=255)
                                                                                                                aTACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCgg                                      <  1:415405/150‑1 (MQ=255)
                                                                                                                  ccggctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCAGGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTAGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCg                                    <  2:1701156/146‑1 (MQ=255)
                                                                                                                   cGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGa                                   >  2:442554/1‑150 (MQ=255)
                                                                                                                     tctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGGTGCACCATCACTTATTCAGGTCAGTGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAg                                 <  1:939880/150‑1 (MQ=255)
                                                                                                                     tctctcGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAg                                 <  1:755125/150‑1 (MQ=255)
                                                                                                                          cgcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATc                            <  1:703068/150‑1 (MQ=255)
                                                                                                                           gcgTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCCTCACTTATTCAGGTCAGAGATGATGTAACGCCTTATCGTTGTTGTTTTGCTTGCCAGCCCGCAGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGCAGGCATCa                           >  1:1106586/1‑150 (MQ=255)
                                                                                                                             gTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaaa                         >  1:1455665/1‑150 (MQ=255)
                                                                                                                              tGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCGGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAAc                        <  2:1091357/150‑1 (MQ=255)
                                                                                                                               gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACa                       <  2:1490314/150‑1 (MQ=255)
                                                                                                                               gAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACa                       <  1:1374398/150‑1 (MQ=255)
                                                                                                                                   tAATGCGGTTGGGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACAtctc                   <  2:1067775/150‑1 (MQ=255)
                                                                                                                                      tGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCTGTTTCGGCGAAGACATCAAACATCTCGGc                <  1:1513508/150‑1 (MQ=255)
                                                                                                                                       gCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCa               >  1:1254272/1‑150 (MQ=255)
                                                                                                                                       gCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCa               >  1:210013/1‑150 (MQ=255)
                                                                                                                                       gCGGTTGTGGTGCGGACTGCAGGCTGCACCATCACTTATTCATGTAAGAGATGATGAAACGCATTATCTTTCTTGTTTTTCTTGCCAGCAAGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTAGGCGAAGACATCAAACATCTCGGCa               <  2:1210723/150‑1 (MQ=255)
                                                                                                                                        cGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCaa              >  2:552648/1‑150 (MQ=255)
                                                                                                                                        cGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCaa              >  2:1558770/1‑150 (MQ=255)
                                                                                                                                              tggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCAt        >  1:1648068/1‑150 (MQ=255)
                                                                                                                                              tggtgCAGCCTGCAGGCCGCACCATCACTTATTCAGGTCAGAGAAGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCAt        >  1:133307/1‑150 (MQ=255)
                                                                                                                                                gtgCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCCGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCAtct      >  1:1617864/1‑150 (MQ=255)
                                                                                                                                                  gCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCaaa                         <  1:1610013/129‑1 (MQ=255)
                                                                                                                                                    ggCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTcg  >  2:1294286/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATACCGCAGGTACTGGTGGCAAACCGGCTACCCTGTCTACTGGCGCTGTGGTTAAAGTTCCGCTGTTTGTACAAATCGGCGAAGTCATCAAAGTGGATACCCGCTCTGGTGAATACGTCTCTCGCGTGAAGTAATGCGGTTGTGGTGCGGCCTGCAGGCTGCACCATCACTTATTCAGGTCAGAGATGATGAAACGCCTTATCGTTCTTGTTTTGCTTGCCAGCACGCTGCTCACGGGCTGTAACACCGCTCGCGGTTTCGGCGAAGACATCAAACATCTCGGCAACTCCATCTCTCG  >  NC_000913/4376132‑4376429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: