| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,401,064 | T→G | 5.7% | H131Q (CAT→CAG) | hflX → | GTPase, stimulated by 50S subunit binding; Mn(2+) homeostasis regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,401,064 | 0 | T | G | 5.7% | 325.3 / 2.7 | 117 | H131Q (CAT→CAG) | hflX | GTPase, stimulated by 50S subunit binding; Mn(2+) homeostasis regulator |
| Reads supporting (aligned to +/- strand): ref base T (49/60); new base G (2/6); total (51/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 117 total aligned reads displayed.
ATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACAGTCGCGCCTGGAAAGAG > NC_000913/4400916‑4401212 | aTGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATc < 1:1021846/150‑1 (MQ=255) tGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGGTGCAGGTTGAGCTGGCGCAGCTGCGCCAGCt < 2:232845/150‑1 (MQ=255) ccTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTTCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGc < 1:213380/150‑1 (MQ=255) ccTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGGTGAGCTGGCGCAGCTGCGCCATCTGGc < 1:1360739/150‑1 (MQ=255) tGAGCCCGGCGCAAGAGCGTAACCTGCAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGATGGCGCAGCTGCGCCAGCtcaacc < 1:626408/150‑6 (MQ=255) cccGGCGCAAGAGCGTAACCTGGCGCGTTTGGGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTAcgcg < 1:1071925/150‑1 (MQ=255) ggCGCAAGAGCGTAACCTGGAGCGTTGGTGCGAGTGGCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCt < 2:1529535/150‑1 (MQ=255) cgcAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTgg < 1:1286647/150‑1 (MQ=255) cgcAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTgg < 1:1616536/150‑1 (MQ=255) cAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGCCCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTg > 2:872565/1‑150 (MQ=255) cAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCGGGCGCAGCTGCGCCATCTGGCCACGCACCAggcg < 2:924064/150‑3 (MQ=255) agagCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAGCGTGCGCGTACCCATGAGTGTAAGTTGCAGTTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCg > 1:268828/1‑150 (MQ=255) agCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTg < 2:1376988/150‑1 (MQ=255) agCGTAACCTGGAGCGTTTGTGCGAGGGTCGTGATATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTg < 2:130022/150‑1 (MQ=255) cGTCACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGCGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGc > 1:1532206/1‑150 (MQ=255) gTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCt > 1:1455925/1‑150 (MQ=255) ccTGGAGCGTTTGGGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGAc < 1:89810/150‑1 (MQ=255) cTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGAcc > 2:1544/1‑150 (MQ=255) tGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCCACGCACCAGGCGCGTAGccagatgg < 1:1388996/150‑8 (MQ=255) tGGAGCGTTTGTGCGAGTGGCGTGGTATCGACCGCACCGGCCTTATTTTAGAAATTTTCGCCCTACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCACCAGGCGCGTAGccagatgg < 1:1723539/150‑8 (MQ=255) cgCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCACCGTGCGCGTACCCATGCGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCAcc < 1:591359/149‑1 (MQ=255) aGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCAcc > 1:1330615/1‑150 (MQ=255) gCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCt < 2:908898/150‑1 (MQ=255) gTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTg < 2:1049177/150‑1 (MQ=255) gTTTGTGAGCGTGACGTGTTATGGCCCGCACCGGCGTTATTTTAGTTAATTTCGCCCAAGGGGCGCGTACGCATGAGGGTAAGTTGCAGGTGGAGCTGGCGCTGCTGCGCCATCTGGCGACGCGCCTGGTGCGTGGCAGGACCCACCTTg < 2:672227/150‑1 (MQ=255) gtgCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAg > 1:1003731/1‑150 (MQ=255) gtgCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAg < 1:824018/150‑1 (MQ=255) gtgCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAg < 1:1133745/150‑1 (MQ=255) gCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGAc > 2:1441352/1‑150 (MQ=255) cGAGGGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGTGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACa < 2:1147704/150‑1 (MQ=255) tgtCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGaaa > 2:1334250/1‑150 (MQ=255) gtTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCggg > 2:1299096/1‑150 (MQ=255) ttATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGa < 1:1544/150‑1 (MQ=255) tATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCAGGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCATGAAAGACAGAAAGGCGGGAt > 2:353097/1‑150 (MQ=255) tATCGACCGCACCGGCCTTATTTTAGATATTATCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGAt < 2:228563/150‑1 (MQ=255) aTCGACCGCACCTGCCTTTTTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATa < 1:477277/150‑1 (MQ=255) cGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGATGGTAAGTTGCAGGTTGAGCTGGCGCTGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAgg < 1:1133862/150‑1 (MQ=255) gACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGAGAGAAAGGCGGGATAGGt > 2:706067/1‑150 (MQ=255) ggCACCGGCCTTATTTTAGATATTTTCGCACTACGGGCGCGTAGCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGACAGGCGGGATAGGTTTg < 2:1118819/149‑1 (MQ=255) cGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTg > 2:544741/1‑150 (MQ=255) gCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCCCCTTGAAAGACAGAAAGGCGGGATAGGTTTGc > 2:515688/1‑150 (MQ=255) gCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGc > 1:519274/1‑150 (MQ=255) gCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGc < 1:850071/150‑1 (MQ=255) cACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCg > 1:374456/1‑150 (MQ=255) aCCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGt < 2:1037964/150‑1 (MQ=255) caggCCTTATTTTTGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCGCCTTGAATGAGAGAAAGGCGGGATAGGTTTGCGTg > 2:41899/3‑150 (MQ=255) cGGCCGTATTTTAGATATTTTCGCCCAACGTGCTCGTACCCATGACGGTAAGTTGAAGGTTGAGCTGTCGCAGCTGCGCCAGCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTgg < 2:265729/150‑1 (MQ=255) tATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCggg < 2:1472006/150‑1 (MQ=255) aTTTTAGATATTTTCGCCCAACGTGCGCGTACCCTTGCGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTTAAAGACATAAAGGCGGGATAGGTTTGCGTGGTCCGGGt < 2:1558964/150‑1 (MQ=255) gATATTTACGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCCGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGACAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAAcc < 2:164236/150‑1 (MQ=255) atatTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAAccc < 1:722003/150‑1 (MQ=255) atatTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCGGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGGGGTCCGGGTGAAAccc < 2:275237/150‑1 (MQ=255) atTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCCGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGATAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAg > 2:1501086/1‑150 (MQ=255) atTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGCAACCCAg > 2:714747/1‑150 (MQ=255) tttCGCCCACCGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCt < 1:679593/150‑1 (MQ=255) gCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGaa > 1:1464986/1‑150 (MQ=255) cccAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGaaa > 1:593821/1‑150 (MQ=255) ccAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAACCCCAGCTCGCAac > 1:1310384/1‑150 (MQ=255) ccAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAac > 1:993393/1‑150 (MQ=255) ccAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAac > 1:407106/1‑150 (MQ=255) ccAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAac > 1:801923/1‑150 (MQ=255) ccAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGGGGAGCTGGCGCAGCTGCTCCAGCTGGCTACGCGCCTGGGGCGGGGCTGGACACACCTTGAAAGACAGAAAGGCGTGCTAGGTTGGCGTGGTCCGGGTGAAACCCAGCTCGAAac < 1:287118/150‑1 (MQ=255) cAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAacc < 1:485850/150‑1 (MQ=255) tGCGCGTACCCATGCGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAaccgaccg < 1:755863/150‑1 (MQ=255) gcgTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACcgtcg > 1:189557/1‑150 (MQ=255) gTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGtt < 2:536671/150‑1 (MQ=255) cccATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTttgt > 1:555518/1‑150 (MQ=255) cATGAGTGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTttgttg < 2:813584/150‑1 (MQ=255) cATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCAGCTGGCTACGCGCCTGGTGCGGGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGGGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTttgttg < 2:1226456/150‑1 (MQ=255) aGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTa > 1:1384990/1‑150 (MQ=255) gggTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGCCAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTttgtatatact > 1:1109450/1‑143 (MQ=255) gggTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTaa > 2:531222/1‑150 (MQ=255) gggTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTaa > 1:584632/1‑150 (MQ=255) gggTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTAGCGTaa > 1:1709681/1‑150 (MQ=255) caaagaagCAGCTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCATGAAAGACAGAAAGGCGGGATAGGTTTTCGTGCTCCGGGTGAAACCCAGCTCGAAAACGACCGACGTTTGTTGCGTAATc > 2:550674/8‑150 (MQ=255) gTTGCAGGGTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCAt < 2:663673/150‑1 (MQ=255) tGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGGGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCg < 2:69041/150‑1 (MQ=255) aGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGCCCGTCGTTTGTTGCGTAATCGCATCGTGc > 1:1627434/1‑150 (MQ=255) aGGGTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATATGTTTGCGTGGGCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGc < 1:866032/150‑1 (MQ=255) tttGAGCTGGCGCAGCTGCGCAATATGGCTAAGCGCCGGGTGCGTGGCTGGACCCACCTCGACAGACAGAAAGGCGGGATAGGCTTGCGTGGTCCGGGGCAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAg < 1:1309990/149‑1 (MQ=255) agccagatGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAg > 2:183901/8‑150 (MQ=255) agccagatGGCGCAGCTGCGCCAGCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCATGAAAGACAGAACGGCGGGATAGGTTTGCGTGGTCCGGTTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAg > 2:1308020/8‑150 (MQ=255) gccagatGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGa > 1:738178/7‑150 (MQ=255) tGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGAt < 1:119181/150‑1 (MQ=255) ccagatGGCGCAGCTGCGCCAGCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGCCCGTCGTTTGTTGCGTAATCGCATCGTGCAGAt > 2:1157186/6‑150 (MQ=255) cgCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATAc < 1:1060563/149‑1 (MQ=255) cgCTGGCGCAGCGGCGCCATCTGGCTACGCGCCTGGGGCGTGGCTGGACCCACCTTGACAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATAc < 1:1065958/149‑1 (MQ=255) aGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGTTAc > 1:1422510/1‑150 (MQ=255) aGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATAc < 2:519274/150‑1 (MQ=255) gCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACa < 1:788876/150‑1 (MQ=255) gCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACa > 2:689644/1‑150 (MQ=255) cTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCGGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGGCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACAg < 2:268828/150‑1 (MQ=255) atGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACAg > 1:631145/2‑150 (MQ=255) ggCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACAGTc > 2:233017/1‑150 (MQ=255) ggCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTAGAACCCGACCGTCGTTTGTTGCGTAATCTCATCGTGCAGATACAGTc > 2:942662/1‑150 (MQ=255) tGCGCCATCTGGCTACTCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACAGTCGCGCCTgg < 1:941072/150‑1 (MQ=255) tGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACAGTCGCGCCTgg < 2:1455925/150‑1 (MQ=255) ggcgcCATCTGGCTACGCGCCTGGTGCGTGGCTGTACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACAGTCGCGCCTgg < 2:307401/149‑1 (MQ=255) ccATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACAGTCGCGCCTGGAAag < 1:1334250/150‑1 (MQ=255) aTCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACAGTCGCGCCTGGAAagag < 1:48316/150‑1 (MQ=255) | ATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGGATAGGTTTGCGTGGTCCGGGTGAAACCCAGCTCGAAACCGACCGTCGTTTGTTGCGTAATCGCATCGTGCAGATACAGTCGCGCCTGGAAAGAG > NC_000913/4400916‑4401212 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |