Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,423,004 C→G 5.5% S53R (AGC→AGG ulaE → L‑xylulose 5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,423,0040CG 5.5% 384.3 / 8.5 123S53R (AGC→AGGulaEL‑xylulose 5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (62/54);  new base G (5/2);  total (67/56)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 128 total aligned reads displayed.

AAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGC  >  NC_000913/4422855‑4423150
                                                                                                                                                      |                                                                                                                                                  
aaacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGc                                                                                                                                                    >  2:1666671/1‑150 (MQ=255)
 aacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCTAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGcc                                                                                                                                                   >  1:658253/1‑150 (MQ=255)
 aacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGcc                                                                                                                                                   >  2:534861/1‑150 (MQ=255)
 aacaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGcc                                                                                                                                                   >  1:43735/1‑150 (MQ=255)
    aaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCcgcg                                                                                                                                                <  2:1058347/150‑1 (MQ=255)
      aTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGGCTCCAGTTGAGGCGCGac                                                                                                                                              <  1:905777/150‑2 (MQ=255)
         cctcgtGGCATCTCTGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGAGTTTGTCGAAAGGTCGGTAGATCATACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGagcag                                                                                                                                           <  1:1576156/145‑1 (MQ=255)
             ttGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc                                                                                                                                       <  2:1017003/150‑1 (MQ=255)
                gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTgg                                                                                                                                    >  2:1481295/1‑150 (MQ=255)
                gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCGGGCTGGAACGCCGGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTgg                                                                                                                                    <  1:1063054/150‑1 (MQ=255)
                 cATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgc                                                                                                                                   <  1:993562/150‑1 (MQ=255)
                 cATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGAGTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgc                                                                                                                                   >  2:1542890/1‑150 (MQ=255)
                   tCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcgc                                                                                                                                 >  2:1595546/1‑150 (MQ=255)
                     tATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGGCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg                                                                                                                               <  2:1001479/150‑1 (MQ=255)
                      aTGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCggg                                                                                                                              >  1:1512597/1‑150 (MQ=255)
                      aTGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTgg                                                                                                                              >  2:1672748/1‑150 (MQ=255)
                        gAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGACACTGACGATCGCCTGTCGCTCCTCAACTGGAGCCGCGCGCAGCGTCTGGCGCTGGTc                                                                                                                            >  2:158767/1‑150 (MQ=255)
                        gAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCACCTGGCGCCGGGAGCAGCGGCTGGCGCTGGTc                                                                                                                            >  2:164183/1‑150 (MQ=255)
                        gAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTc                                                                                                                            >  1:619571/1‑150 (MQ=255)
                        gAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATGTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTc                                                                                                                            >  2:100943/1‑150 (MQ=255)
                         aaaaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCa                                                                                                                           >  2:33782/1‑150 (MQ=255)
                          aaaaGCGCTCCCCGACGGGGAGTGCTGGCTGGAACGCCTGCAAATTGCAAAAACGTTAGGCTTCTAATTTGTCGAAATGTCTGTATATGAAAATTACGATAGCCTGTCGAGCCTCAACTGGAGCCGCGACCATAGTCTGGCGCTTGTCaa                                                                                                                          >  2:611029/1‑150 (MQ=255)
                           ccaGCACTCCCCGGCGGGGAGCGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGAAGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCGGGTCAAt                                                                                                                         >  2:1270902/3‑150 (MQ=255)
                            caGCACTCCCCGGCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATg                                                                                                                        >  2:400093/2‑150 (MQ=255)
                                gcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGa                                                                                                                     >  1:1299726/1‑150 (MQ=255)
                                gcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAt                                                                                                                    >  2:1250512/1‑150 (MQ=255)
                                gcTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGAACGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAt                                                                                                                    <  2:6467/150‑1 (MQ=255)
                                gcTCCCCGCCGGGGAGTGCTGGCTGGAACGACTGCAACTGGCAAAAACGTTAGGCATCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGAGCTGGTCAATGCGAt                                                                                                                    >  1:1121640/1‑150 (MQ=255)
                                gcTCCCCGCCGGGGAGTGCTGGCTGGAACCCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAt                                                                                                                    >  2:708955/1‑150 (MQ=255)
                                 cTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAtt                                                                                                                   >  1:1265707/1‑150 (MQ=255)
                                  tCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAACCTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttg                                                                                                                  >  1:1439460/1‑150 (MQ=255)
                                   ccccGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttgt                                                                                                                 >  2:317225/1‑150 (MQ=255)
                                                  ctggctggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCTATTGTTGAAACCGgcgtgcg                                                                                                  >  2:121867/1‑150 (MQ=255)
                                                    ggctggAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATTGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGgcgtgcgcg                                                                                                <  1:681335/150‑1 (MQ=255)
                                                             gCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCa                                                                                       <  1:1024617/150‑1 (MQ=255)
                                                               cTGCAACTGGCAAAATCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGGCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAtg                                                                                     <  1:714510/150‑1 (MQ=255)
                                                               cTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAtg                                                                                     <  2:916364/150‑1 (MQ=255)
                                                                 gCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCAtgtg                                                                                   >  1:529320/1‑150 (MQ=255)
                                                                  cAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGc                                                                                  <  1:317225/150‑1 (MQ=255)
                                                                  cAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGc                                                                                  <  2:1379624/150‑1 (MQ=255)
                                                                   aaCTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGcc                                                                                 <  1:1416014/150‑1 (MQ=255)
                                                                      tGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACCGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCttt                                                                              >  2:1596154/1‑150 (MQ=255)
                                                                       ggCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGCGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCttta                                                                             >  2:1502295/1‑149 (MQ=255)
                                                                       ggCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTc                                                                             >  2:314375/1‑150 (MQ=255)
                                                                         cAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTg                                                                           >  1:484221/1‑150 (MQ=255)
                                                                         cAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGAGAGCAGCGTCAGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTg                                                                           >  2:203323/1‑150 (MQ=255)
                                                                          aaaaaCGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGc                                                                          >  2:818607/1‑150 (MQ=255)
                                                                                  tAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                                                                  >  2:229288/1‑150 (MQ=255)
                                                                                  tAGGCTGCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtc                                                                  <  1:881141/150‑1 (MQ=255)
                                                                                   aGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAACCCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                                                                 >  2:475811/1‑150 (MQ=255)
                                                                                    ggCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                                                                 >  2:1413578/1‑149 (MQ=255)
                                                                                    ggCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCAtcgtcg                                                                 <  1:1413578/149‑1 (MQ=255)
                                                                                      cTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGttt                                                              >  1:1044867/1‑150 (MQ=255)
                                                                                        tCGATTTTGTCGATATGTGGGTAGAGGAATCTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGTGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCTTCGTCGTTTcc                                                            <  1:901309/150‑1 (MQ=255)
                                                                                        tCGACTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGAGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTcc                                                            <  1:895252/150‑1 (MQ=255)
                                                                                          gATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCg                                                          <  1:187565/150‑1 (MQ=255)
                                                                                           aTTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGc                                                         <  1:227385/150‑1 (MQ=255)
                                                                                               tgacGAACTGTCGGTCGATGAAACTGACGATCGACTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAACCCGGCGTGCGCGTGCCGTCCATGAGCCTTTCTGCTCATCGTCGTTTCCCGCTggg                                                     <  1:101637/147‑1 (MQ=255)
                                                                                               tGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTggg                                                     <  2:2049/150‑1 (MQ=255)
                                                                                                gTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGc                                                    >  1:1136465/1‑150 (MQ=255)
                                                                                                 tCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCa                                                   >  1:381469/1‑150 (MQ=255)
                                                                                                  cGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGCGCCGCGTCTGGCGCGGGTCACTTCGAGTGTTGAAACCGGCGGGAGCGTGCCGGCCATGTGCCGTTCTGCTCCTCGTCGTGTCCCGCTGGGCAg                                                  >  2:286148/1‑150 (MQ=255)
                                                                                                   gAAATGTCGGTAGATGAAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGCCGTTTCCCGCTGGGCAg                                                  <  2:1299726/150‑1 (MQ=255)
                                                                                                   gAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGGTGGGctgg                                                 >  1:1160707/1‑147 (MQ=255)
                                                                                                       tGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAg                                             <  2:1549088/150‑1 (MQ=255)
                                                                                                        gTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGGGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGa                                            >  1:1019805/1‑150 (MQ=255)
                                                                                                          cGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATg                                          >  2:1132413/1‑150 (MQ=255)
                                                                                                          cGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATg                                          >  1:1723978/1‑150 (MQ=255)
                                                                                                           ggTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCTCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGa                                         >  2:1452290/1‑150 (MQ=255)
                                                                                                            gaaGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGAc                                        <  2:934554/148‑1 (MQ=255)
                                                                                                                  gAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTg                                  <  2:51928/150‑1 (MQ=255)
                                                                                                                       cTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCggg                              >  1:1389453/1‑150 (MQ=255)
                                                                                                                       cTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCggg                              <  1:1481295/150‑1 (MQ=255)
                                                                                                                       cTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCggg                              >  1:423881/1‑150 (MQ=255)
                                                                                                                        tGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGgc                             <  1:252559/150‑1 (MQ=255)
                                                                                                                         gACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGgcg                            >  2:406147/1‑150 (MQ=255)
                                                                                                                           cGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCa                          >  2:1596069/1‑150 (MQ=255)
                                                                                                                            gATCGCCTGTCGCGCCGCAACTGGAGCCGCGAGCTGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATTTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAg                         <  2:1051841/150‑1 (MQ=255)
                                                                                                                              tCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAggg                       <  1:1270902/150‑1 (MQ=255)
                                                                                                                              tCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGGCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAggg                       <  2:1265707/150‑1 (MQ=255)
                                                                                                                               cGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAgggg                      <  2:1439460/150‑1 (MQ=255)
                                                                                                                                gCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGc                     <  1:415288/150‑1 (MQ=255)
                                                                                                                                gCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCGGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGc                     >  1:1452610/1‑150 (MQ=255)
                                                                                                                                   tGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTgg                  <  2:43735/150‑1 (MQ=255)
                                                                                                                                   tGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTgg                  <  2:1055927/150‑1 (MQ=255)
                                                                                                                                   gctCGCGGCTCCAGTTGAGGCGCGATCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGTCGTGCGCGTGCAGTCCATGTGCCTTTCTGCTCATAGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCTGGCGCAGGGGCTgg                  >  2:241555/3‑150 (MQ=255)
                                                                                                                                    gTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCAGCTCATCGTCGTTTCCCGCTGGGCAGTGAACATGACCCGGTGCGGGCGCAGGGGCTggt                 >  1:793647/1‑149 (MQ=255)
                                                                                                                                    ctCGCGGCTCGAGTTGAGGCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGga                 >  1:610870/2‑150 (MQ=255)
                                                                                                                                    ctCGCGGCTCCAGTTGAGGCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCGTCGTCGTTTCCCGCTGGGCAGTGAAGATGACCCGGTGCGGGCGCAGGGGCgggg                 >  1:170657/2‑149 (MQ=255)
                                                                                                                                     tCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGgag                <  1:502928/150‑1 (MQ=255)
                                                                                                                                           cTCAACTGGAGCCGCGAGCAGCGTCTGGCGGTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTGATCGTCGTTTCCCGCAGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCAGGAGATTATg          >  1:778245/1‑150 (MQ=255)
                                                                                                                                            gcAACTGGAGCCGCGAGCAGCGGCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGc         <  1:1562563/149‑1 (MQ=255)
                                                                                                                                             cAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGTCGTGCGCGTGCCGTCCATGTGCCTTTATGCTCATCGTCGTTTACCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCg        >  2:398134/1‑150 (MQ=255)
                                                                                                                                             cAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCg        <  1:314375/150‑1 (MQ=255)
                                                                                                                                             cAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATAGTCGTTTACCGCTGGGCAGTGAAGATGAAGAGGTGCGTGCGCAGGGGCTGGAGATTATGCg        >  2:391938/1‑150 (MQ=255)
                                                                                                                                              aaCTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGt       <  1:1250512/150‑1 (MQ=255)
                                                                                                                                               aCTGGAGCCGCGCGCAGCGTCGGGCGCTGGTCAATGCGATTGTTGAACCCTGCGTGCGCGTGCCCTCCATGTGCCTTTCTGCTCAGCGTAGTTTCCCGCTGGGCAGTGAAGAGGACGCGGTGCGGGCGCTGGGGCTGGAGATGATGCgga      >  2:671679/1‑148 (MQ=255)
                                                                                                                                               aCTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTa      <  2:484221/150‑1 (MQ=255)
                                                                                                                                                   gAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGc  <  2:946064/150‑1 (MQ=255)
                                                                                                                                                   gAGCCGCGAGCAGCGGCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGc  <  1:1542890/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
AAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGGCGTGCGCGTGCCGTCCATGTGCCTTTCTGCTCATCGTCGTTTCCCGCTGGGCAGTGAAGATGACGCGGTGCGGGCGCAGGGGCTGGAGATTATGCGTAAAGC  >  NC_000913/4422855‑4423150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: