Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,429,127 A→T 5.8% I65F (ATC→TTC) ‡ fklB → FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,1270AT 5.8% 362.1 / 8.7 121I65F (ATC→TTC) ‡fklBFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base A (61/53);  new base T (2/5);  total (63/58)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

Only 100 of 123 total aligned reads displayed.

CGGCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGG  >  NC_000913/4428979‑4429275
                                                                                                                                                     |                                                                                                                                                     
tgccATTGGTTTGCAGGTAGGGCAACAAGTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCATAAGCACAGGTTGCAGGTATTGCCGATGCGCTGGAAGGCTAACATCCGGCTGTTCCGGATGATGTGGTGCAGCGCGCGCTGCGTGAAAt                                                                                                                                                      <  1:825544/147‑1 (MQ=255)
 ggCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATc                                                                                                                                                     >  1:872029/1‑150 (MQ=255)
       ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAATTGCTCCTGCGCGAAGAACTGGTTGCAGGTATTGCGGATGCTCTGGAAGGCAAAAATCAGGCTGATAAGGTTGATGTGGTGCATCGCGCTCTGCGAGAAATCCACGAg                                                                                                                                              >  1:238891/1‑150 (MQ=255)
       ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAg                                                                                                                                              >  1:939050/1‑150 (MQ=255)
       ggTGTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCGGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAg                                                                                                                                              <  1:976236/150‑1 (MQ=255)
         gttGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAgcg                                                                                                                                            <  1:1298192/149‑1 (MQ=255)
              aGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAgcgcgcga                                                                                                                                       <  1:735538/150‑3 (MQ=255)
              aGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAgcgcgcg                                                                                                                                        <  2:907940/150‑2 (MQ=255)
                gTAGGGCAACAACTGTGTGAATCTGGCCTGTACGGGCTGCTGCCAGAATCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGAt                                                                                                                                     <  1:1131021/150‑1 (MQ=255)
                gTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGAt                                                                                                                                     <  1:625323/150‑1 (MQ=255)
                 tAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATg                                                                                                                                    >  1:96521/1‑150 (MQ=255)
                 gaGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCTGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATg                                                                                                                                    <  2:147649/149‑1 (MQ=255)
                  aGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCGATGc                                                                                                                                   <  1:210951/150‑1 (MQ=38)
                    ggCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCg                                                                                                                                 >  1:1238832/1‑150 (MQ=255)
                       aacaacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCGATGcaccac                                                                                                                              <  2:1698508/150‑6 (MQ=37)
                       aacaacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCGATGcaccac                                                                                                                              <  2:1691953/150‑6 (MQ=25)
                        acaacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcg                                                                                                                             >  2:789893/1‑150 (MQ=255)
                        acaacTGAGGGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTGCCGGTTGATGTGGTGCATCGCGCGCTTCGTGAAATCCACGAGCGCGCCGATGCCGTtcg                                                                                                                             <  1:1274358/150‑1 (MQ=255)
                          aacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTGGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcgtc                                                                                                                           <  1:1357971/150‑1 (MQ=255)
                           acTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcgtcg                                                                                                                          <  1:490072/150‑1 (MQ=255)
                           acTGAGTGAAGCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcgtcg                                                                                                                          <  2:1483902/150‑1 (MQ=255)
                             tGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCgtcgtc                                                                                                                        >  1:426520/1‑150 (MQ=255)
                             tGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCgtcgtc                                                                                                                        >  1:183299/1‑150 (MQ=255)
                              gAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCgtcgtcc                                                                                                                       >  1:279445/1‑149 (MQ=255)
                               aGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAg                                                                                                                      <  1:1250793/150‑1 (MQ=255)
                                gTGAATCTGGCCTGGAAGTGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGc                                                                                                                     >  2:215912/1‑150 (MQ=255)
                                 tGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCg                                                                                                                    <  2:685726/150‑1 (MQ=255)
                                   aaTCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGAGtt                                                                                                                  >  2:253906/1‑150 (MQ=255)
                                    aTCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGttt                                                                                                                 >  2:892125/1‑150 (MQ=255)
                                      cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                               >  2:246885/1‑150 (MQ=255)
                                         gCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                            >  2:1686936/1‑150 (MQ=255)
                                           cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCg                                                                                                          >  2:116619/1‑150 (MQ=255)
                                           cTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCg                                                                                                          <  2:804448/150‑1 (MQ=255)
                                              gAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGAGGCGCTGGAAGGCAAACATCCGGCAGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGTAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATg                                                                                                       <  1:1503727/150‑1 (MQ=255)
                                                aGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgc                                                                                                     >  1:1276797/1‑150 (MQ=255)
                                                  ggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAATGCAAACATACGGCTGTACCCGTTGATGAGGTGCATCCCGCCCTGCGTGAAATCCACGAGCGCGCCGATCCCGTACTTCGTCAGCGTGTCCAGGCGATGgcag                                                                                                   >  2:672493/1‑148 (MQ=255)
                                                  ggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctg                                                                                                   >  1:773501/1‑150 (MQ=255)
                                                  ggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctg                                                                                                   >  1:61162/1‑150 (MQ=255)
                                                   gctgctGCCAGAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctgc                                                                                                  <  1:1726774/150‑1 (MQ=255)
                                                    ctgctgCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGATTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctgct                                                                                                 <  2:387379/150‑1 (MQ=255)
                                                      gctgcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGa                                                                                               >  1:1731038/1‑150 (MQ=255)
                                                       ctgcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGaa                                                                                              >  1:521344/1‑150 (MQ=255)
                                                        tgcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAg                                                                                             >  1:1689896/1‑150 (MQ=255)
                                                         gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                            <  2:613703/150‑1 (MQ=255)
                                                         gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                            >  2:899083/1‑150 (MQ=255)
                                                           cAGAAGCTCTGGTGGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtg                                                                                          <  2:481774/150‑1 (MQ=255)
                                                           cAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCTCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtg                                                                                          >  2:1113529/1‑150 (MQ=255)
                                                            aGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtgt                                                                                         >  1:1222075/1‑150 (MQ=255)
                                                                gCACTGGTTGCAGGTTTTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGaaa                                                                                     <  1:357602/150‑1 (MQ=255)
                                                                 cACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGAGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAt                                                                                    <  2:279445/150‑1 (MQ=255)
                                                                  aCTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCAATGGCTGCTGAAGGTGTGAAATa                                                                                   >  1:370623/1‑150 (MQ=255)
                                                                      gTTGCAGGTATTGCCGATGCGCTGGCAGGCAAACATCCGGCTGTTCCGGTGGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                               <  1:266429/150‑1 (MQ=255)
                                                                       ttGCAGGTATTGCCGTTGCGCTGGAAGGCATACATCCGGCTGTGCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTgg                                                                              <  2:939050/150‑1 (MQ=255)
                                                                          cAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaag                                                                           >  1:1025160/1‑150 (MQ=255)
                                                                            ggTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                            >  1:1251258/1‑115 (MQ=255)
                                                                            ggTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                            <  2:1251258/115‑1 (MQ=255)
                                                                            ggTAGTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaagaa                                                                         <  1:56367/150‑1 (MQ=255)
                                                                                ttGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACg                                                                     <  1:1590548/150‑1 (MQ=255)
                                                                                  gCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGcc                                                                   >  1:1664486/1‑150 (MQ=255)
                                                                                  gCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGcc                                                                   >  1:1641175/1‑150 (MQ=255)
                                                                                     gATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaaa                                                                >  2:1195070/1‑150 (MQ=255)
                                                                                       tGCGCTGGAATGCAAACATCCGGCTGTTCCGGTTGATGTTGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaaaaa                                                              <  2:387590/150‑1 (MQ=255)
                                                                                         cgcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaag                                                            >  1:24778/1‑150 (MQ=255)
                                                                                           cTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGAGCATCGCTCGCTGCGTGAAATCCAAGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaa                                                          >  1:62604/1‑150 (MQ=255)
                                                                                            tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                         <  2:1042164/150‑1 (MQ=255)
                                                                                             ggAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAgg                                                        <  1:708023/150‑1 (MQ=255)
                                                                                                 ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                    >  1:1673940/1‑150 (MQ=255)
                                                                                                 ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATCCCGTTCGTCGTCAGCGTTTCCATGCGATGGCTGCTGAAGGGGTGAACTACCTCCAAGCAAACCCCAACCACGCAGGtgtg                                                    >  1:301213/1‑150 (MQ=255)
                                                                                                   cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGaa                                                  <  1:1686936/150‑1 (MQ=255)
                                                                                                    aaaCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAAt                                                 >  1:618551/1‑150 (MQ=255)
                                                                                                           cGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGTTGGCTGCTGAAGGTGTGAATTACCGGGAAGAAAACGCCAAAACAGAAGGTGGGCTTAGCCCCg                                          >  1:1136541/1‑150 (MQ=255)
                                                                                                           cGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCg                                          <  1:1113529/150‑1 (MQ=255)
                                                                                                            ggaacagccGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGa                                         >  2:218932/8‑150 (MQ=255)
                                                                                                            ggCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGa                                         >  1:1129311/1‑150 (MQ=255)
                                                                                                            ggCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGa                                         >  2:669456/1‑150 (MQ=255)
                                                                                                                gTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCt                                     <  1:1483105/150‑1 (MQ=255)
                                                                                                                    cGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCCCGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGAGGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGcc                                 <  2:130917/150‑1 (MQ=255)
                                                                                                                    cGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGcc                                 <  1:496225/150‑1 (MQ=255)
                                                                                                                        tGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGCGCGCGGCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCa                             >  1:695063/1‑150 (MQ=255)
                                                                                                                         gATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCaa                            >  2:486667/1‑150 (MQ=255)
                                                                                                                          aTGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAAACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTCGCCTGCAAt                           >  2:467315/1‑150 (MQ=255)
                                                                                                                              ggtgCATCGCGCGCTGAGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCATAAAAGAAGGTGTTAATAGCACCGATTCTGGCCTGCAATTCcg                       <  1:368679/150‑1 (MQ=255)
                                                                                                                                  catcggcgcgcTCGTGGATTTCACGCAGCGCGCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTg                   >  1:246269/6‑150 (MQ=38)
                                                                                                                                   atcggcgcgcTCGTGGATTTCACGCAGCGCGAAGATGCCGTTCGTCGTCATAGTTTCCAGGCGATGGCTGCTGAAGGTGTGAATTACCTGGAAGAAAACGACAAAATAGAAGGTGTGAATATCAACGAATCTGTCCTGCAATTCCTCGTg                   >  1:954250/5‑150 (MQ=255)
                                                                                                                                   aTCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGa                  <  2:521344/150‑1 (MQ=255)
                                                                                                                                    tCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                 <  2:773501/150‑1 (MQ=255)
                                                                                                                                    gcgcgcgCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGAt                 <  2:719151/149‑1 (MQ=255)
                                                                                                                                       gcgcgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCa               >  2:421023/1‑150 (MQ=255)
                                                                                                                                          cgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAAcc            <  1:700409/150‑1 (MQ=255)
                                                                                                                                           gcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCa           >  1:1454549/1‑150 (MQ=255)
                                                                                                                                             ggCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAgg         <  1:215912/149‑1 (MQ=255)
                                                                                                                                              gagTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCTGGCGATGGCTGAGGCAGGTGTGAAATACCTGGAAGACAACGCCAAAAAAGCAGGTGTGAATAGGAACGGATCTGGCCGGCCAGGCCGCGTGCGCATCCAggg        >  2:836438/3‑150 (MQ=255)
                                                                                                                                               ggTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTTAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAAGGt       <  1:116619/149‑1 (MQ=255)
                                                                                                                                                gTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTg      >  1:814802/1‑150 (MQ=255)
                                                                                                                                                gTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGAGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTg      >  2:1116009/1‑150 (MQ=255)
                                                                                                                                                gTGAAATCCACGAGCGCGCCGAGGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTg      <  1:1165511/150‑1 (MQ=255)
                                                                                                                                                 tGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGa     >  1:422290/1‑150 (MQ=255)
                                                                                                                                                  gAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGaa    >  2:40152/1‑150 (MQ=255)
                                                                                                                                                    aaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAgg  <  2:167621/150‑1 (MQ=255)
                                                                                                                                                    aaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTGCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCTATTCCGCGTGATCAACCAGGGTGAAgg  <  1:899083/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCATTGGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGG  >  NC_000913/4428979‑4429275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: