Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,460,477 A→C 5.3% intergenic (‑113/+45) nrdG ← / ← nrdD anaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,460,4770AC 5.3% 379.6 / 6.7 125intergenic (‑113/+45)nrdG/nrdDanaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base A (58/60);  new base C (2/5);  total (60/65)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

GACGATGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACA  >  NC_000913/4460329‑4460625
                                                                                                                                                    |                                                                                                                                                    
gACGATGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAg                                                                                                                                                     >  1:706294/1‑150 (MQ=255)
  cGATGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGc                                                                                                                                                   <  1:37375/150‑1 (MQ=255)
  cGAGGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGc                                                                                                                                                   <  1:325856/150‑1 (MQ=255)
     tGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGtt                                                                                                                                                <  2:1487454/150‑1 (MQ=255)
     tGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATCGCCAAATGTAGGCCGGATAAGGCGtt                                                                                                                                                <  2:307674/150‑1 (MQ=255)
      gTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGttt                                                                                                                                               <  2:980154/150‑1 (MQ=255)
      gTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGttt                                                                                                                                               <  1:879514/150‑1 (MQ=255)
           cAGGATAGTACTGAGGATAATTCATTATTTCGCCTGGCTTATCAGACCGCTTCTAGGCTGCATACGGTATTCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAAGTGCCAAATGTCGGCCGGATAAGGAGATTAcgcc                                                                                                                                          >  1:822797/1‑150 (MQ=38)
             ggATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                        >  2:947751/1‑150 (MQ=255)
                tAGTACTGATGATACTTCCTTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCACTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                     <  2:609242/150‑1 (MQ=255)
                tAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATGCGGCGTAAAcgccttatc                                                                                                                                     <  2:1464104/150‑6 (MQ=38)
                 aGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                    <  2:497653/150‑1 (MQ=38)
                   tACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCTTCCGGTACTCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                  <  2:1124081/150‑1 (MQ=38)
                    aCTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTACCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                 <  1:985076/150‑1 (MQ=255)
                       gatgatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt                                                                                                                              >  1:778160/1‑150 (MQ=255)
                       gatgatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt                                                                                                                              <  2:880163/150‑1 (MQ=255)
                       gatgatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt                                                                                                                              >  2:983557/1‑150 (MQ=255)
                       gatgagAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGTTAATCAATGCCTGATGCGACGCTGTCGCGTATTATCAGACCTGGAGCAAATGCAAAATGTAGGCCGGCTAAGGCGTATACGACGCATCCGGCAtt                                                                                                                              >  1:671564/1‑150 (MQ=38)
                        ttgatAATTCTTTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCTTCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt                                                                                                                             <  2:794001/149‑1 (MQ=255)
                        atgatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcctac                                                                                                                             <  2:1564153/150‑5 (MQ=38)
                          gatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGc                                                                                                                           <  2:145133/150‑1 (MQ=255)
                           atAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCACATCCGGCATTTGCt                                                                                                                          <  2:1223254/150‑1 (MQ=255)
                            tAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCtt                                                                                                                         <  2:543126/150‑1 (MQ=255)
                               ttCATTATTTCGCCTGTCTTATCAGTCCGTGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGc                                                                                                                      <  1:141328/150‑1 (MQ=255)
                               ttCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGc                                                                                                                      >  2:1070573/1‑150 (MQ=255)
                                tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                     >  1:1189633/1‑150 (MQ=255)
                                  attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                   >  1:490898/1‑150 (MQ=255)
                                     attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGCCCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                >  2:525138/1‑150 (MQ=255)
                                     attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                >  2:844673/1‑150 (MQ=255)
                                     attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                <  1:1460794/150‑1 (MQ=255)
                                     attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                <  2:1630647/150‑1 (MQ=255)
                                     attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                >  1:153222/1‑150 (MQ=255)
                                         cGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAAAGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGACGGATAAGTCGTTTACGCCGCATACGGCATTTGCTTAGCTCAGAGTGaa                                                                                                            >  2:282060/1‑150 (MQ=255)
                                            cTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGAt                                                                                                         >  1:1316667/1‑150 (MQ=255)
                                            cTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTGCTTAGCTCAGAGTGAAGAt                                                                                                         >  2:339233/1‑150 (MQ=255)
                                             tGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGAtt                                                                                                        >  2:1314565/1‑150 (MQ=255)
                                               gCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTaa                                                                                                      >  2:1476625/1‑150 (MQ=255)
                                                tttctCAGTCCGAGTGTAGGCTGCATACGGTAATCAATGCCTTATGCGACGCTGTCGAGTCTTATCAGACATGGAGCAATTTCCAAATGTAGGCCTGATAAGGCGTTTACGCCGCATCAGGCATTTGCTTAGCTCAGAGTGAAGATTAAc                                                                                                     <  1:1113699/146‑1 (MQ=38)
                                                 ttATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAAcc                                                                                                    >  2:1730996/1‑150 (MQ=255)
                                                 ttAGCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTaagc                                                                                                    <  2:1158950/150‑3 (MQ=255)
                                                       gTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTg                                                                                              >  1:782201/1‑150 (MQ=255)
                                                       gTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTg                                                                                              >  1:488955/1‑150 (MQ=255)
                                                         ccGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGcc                                                                                            <  2:837709/150‑1 (MQ=255)
                                                         ccGCGTGTAGGCTGCATCCGGTAATCAATCCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGGTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGcc                                                                                            <  1:1265794/150‑1 (MQ=255)
                                                              tgtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTc                                                                                       >  2:1599077/1‑150 (MQ=255)
                                                              tgtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTc                                                                                       >  2:633514/1‑150 (MQ=255)
                                                               gtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTcc                                                                                      >  1:435050/1‑150 (MQ=255)
                                                                tatgCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTccc                                                                                     <  2:124558/147‑1 (MQ=255)
                                                                 aGGCTTCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTcccc                                                                                    <  1:1714263/150‑1 (MQ=255)
                                                                 aGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGCGAAGATTAACCTATCTGCCCATTcccc                                                                                    >  1:1727341/1‑150 (MQ=255)
                                                                    cTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCCCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTACCCaaa                                                                                 >  1:405909/1‑150 (MQ=255)
                                                                      gCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATg                                                                               <  1:905117/150‑1 (MQ=255)
                                                                      gCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATg                                                                               >  2:551110/1‑150 (MQ=255)
                                                                      gCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCAGCCGGCATTTGCTTAGCTCAGCGTGAAGATTAACCTATCTGCCCATTCCCCAAATg                                                                               >  2:1555215/1‑150 (MQ=255)
                                                                         tCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGttt                                                                            <  2:687855/150‑1 (MQ=255)
                                                                          ccGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATACGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTa                                                                           >  1:156007/1‑150 (MQ=255)
                                                                           cGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTaa                                                                          >  1:1173881/1‑150 (MQ=255)
                                                                              tAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAAcgc                                                                       <  2:1526336/150‑1 (MQ=255)
                                                                                   aaTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGATGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCTgcgc                                                                  <  2:1074048/150‑1 (MQ=255)
                                                                                   aaTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGgcgc                                                                  >  1:385509/1‑150 (MQ=255)
                                                                                      gCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTa                                                               <  2:26973/150‑1 (MQ=255)
                                                                                         tGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTTAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAAct                                                            <  1:1381354/150‑1 (MQ=255)
                                                                                            tGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAActtct                                                         >  2:448071/1‑150 (MQ=255)
                                                                                               gACGCTGTCGCGTCTTATCAGACCTGGAGCCATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCCTTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTcc                                                      <  1:919724/150‑1 (MQ=255)
                                                                                               gACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTcc                                                      <  1:633514/150‑1 (MQ=255)
                                                                                                   cTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCt                                                  >  2:41169/1‑150 (MQ=255)
                                                                                                    acacgcgTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCtt                                                 >  2:1006633/4‑150 (MQ=255)
                                                                                                       cgcgTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATAAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTAcc                                              >  1:219796/1‑150 (MQ=255)
                                                                                                          gTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGc                                           <  1:542191/150‑1 (MQ=255)
                                                                                                           tCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCg                                          >  2:388992/1‑150 (MQ=255)
                                                                                                            cTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACAAGCGt                                         >  1:1516749/1‑150 (MQ=255)
                                                                                                            attATCAGACCTGGAGCAATTGCCAAATGTTGTGCTTATAAGGCGTTTACGACGCATCCGGCAATTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAAGGTTAAACGCTGCGCTTAACTTCTTCCTGCTTACCAGCGt                                         <  1:266196/149‑1 (MQ=255)
                                                                                                             ttATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGCGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGtt                                        <  2:1727341/150‑1 (MQ=255)
                                                                                                              tATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTCGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTa                                       <  1:1175730/150‑1 (MQ=255)
                                                                                                              tATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTa                                       >  1:1665937/1‑150 (MQ=255)
                                                                                                                 cAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAAc                                    <  1:983557/150‑1 (MQ=255)
                                                                                                                  aGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAAc                                                             <  1:118830/124‑1 (MQ=255)
                                                                                                                  aGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAAc                                                             >  2:118830/1‑124 (MQ=255)
                                                                                                                  aGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACg                                   <  1:1200924/150‑1 (MQ=255)
                                                                                                                      cTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCTCTTAAATTCTTCCTGCTTACCAGCGTTAAACGGACg                               >  2:150627/1‑150 (MQ=255)
                                                                                                                      cTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACg                               <  2:488955/150‑1 (MQ=255)
                                                                                                                       tGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTCCCAGCGTTAAACGGACGt                              >  2:533448/1‑150 (MQ=255)
                                                                                                                       tGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGt                              <  1:383584/150‑1 (MQ=255)
                                                                                                                        ggAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCATATTGAATATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTg                             <  1:282060/150‑1 (MQ=255)
                                                                                                                        ggAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTg                             <  2:221974/150‑1 (MQ=255)
                                                                                                                        ggAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTg                             <  2:1189633/150‑1 (MQ=255)
                                                                                                                           gCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCAt                          >  1:1184343/1‑150 (MQ=255)
                                                                                                                             aaTTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATcc                        >  1:540524/1‑150 (MQ=255)
                                                                                                                              aTTGCCAAATGTAGGCTGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCgg                      <  1:1320041/150‑1 (MQ=255)
                                                                                                                                tGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCggg                     <  1:1476625/150‑1 (MQ=255)
                                                                                                                                  ccAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCt                   <  1:947751/150‑1 (MQ=255)
                                                                                                                                  ccAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCt                   >  2:562563/1‑150 (MQ=255)
                                                                                                                                   cAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTa                  >  2:1010071/1‑150 (MQ=255)
                                                                                                                                      aTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCt               >  1:982734/1‑150 (MQ=255)
                                                                                                                                        gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTaa             >  1:692375/1‑150 (MQ=255)
                                                                                                                                        aaatgCCGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTaa             >  1:1428695/5‑150 (MQ=255)
                                                                                                                                         tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTaaa            <  1:1620697/150‑1 (MQ=255)
                                                                                                                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAatat         >  2:491656/1‑150 (MQ=255)
                                                                                                                                                gATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGc     >  1:733675/1‑150 (MQ=255)
                                                                                                                                                   aaGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGcaca  >  2:1471003/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GACGATGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACA  >  NC_000913/4460329‑4460625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: