Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,460,483 T→A 6.0% intergenic (‑119/+39) nrdG ← / ← nrdD anaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,460,4830TA 6.0% 368.6 / 6.8 124intergenic (‑119/+39)nrdG/nrdDanaerobic ribonucleotide reductase activating protein/anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base T (61/55);  new base A (3/5);  total (64/60)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

TGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACGCGGCG  >  NC_000913/4460334‑4460632
                                                                                                                                                     |                                                                                                                                                     
tGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGtt                                                                                                                                                       <  2:1487454/150‑1 (MQ=255)
tGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATCGCCAAATGTAGGCCGGATAAGGCGtt                                                                                                                                                       <  2:307674/150‑1 (MQ=255)
 gTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGttt                                                                                                                                                      <  2:980154/150‑1 (MQ=255)
 gTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGttt                                                                                                                                                      <  1:879514/150‑1 (MQ=255)
      cAGGATAGTACTGAGGATAATTCATTATTTCGCCTGGCTTATCAGACCGCTTCTAGGCTGCATACGGTATTCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAAGTGCCAAATGTCGGCCGGATAAGGAGATTAcgcc                                                                                                                                                 >  1:822797/1‑150 (MQ=38)
        ggATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                               >  2:947751/1‑150 (MQ=255)
          aTAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATGCGGCGTAAAcgccttat                                                                                                                                             <  2:1477599/150‑5 (MQ=38)
           tAGTACTGATGATACTTCCTTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCACTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                            <  2:609242/150‑1 (MQ=255)
           tAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATGCGGCGTAAAcgccttatc                                                                                                                                            <  2:1464104/150‑6 (MQ=38)
            aGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                           <  2:497653/150‑1 (MQ=38)
               aCTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGACAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                        >  1:772926/1‑150 (MQ=255)
               aCTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTACCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                        <  1:985076/150‑1 (MQ=255)
                  gatgatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt                                                                                                                                     >  1:778160/1‑150 (MQ=255)
                  gatgatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt                                                                                                                                     <  2:880163/150‑1 (MQ=255)
                  gatgatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt                                                                                                                                     >  2:983557/1‑150 (MQ=255)
                  gatgagAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGTTAATCAATGCCTGATGCGACGCTGTCGCGTATTATCAGACCTGGAGCAAATGCAAAATGTAGGCCGGCTAAGGCGTATACGACGCATCCGGCAtt                                                                                                                                     >  1:671564/1‑150 (MQ=38)
                    tgatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTg                                                                                                                                   <  1:1313326/150‑1 (MQ=255)
                     gatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGCTGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGc                                                                                                                                  <  1:1729556/150‑1 (MQ=255)
                     gatAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGc                                                                                                                                  <  2:145133/150‑1 (MQ=255)
                      atAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCACATCCGGCATTTGCt                                                                                                                                 <  2:1223254/150‑1 (MQ=255)
                       tAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCtt                                                                                                                                <  2:543126/150‑1 (MQ=255)
                          ttCATTATTTCGCCTGTCTTATCAGTCCGTGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGc                                                                                                                             <  1:141328/150‑1 (MQ=255)
                          ttCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGc                                                                                                                             >  2:1070573/1‑150 (MQ=255)
                           tCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCt                                                                                                                            >  1:1189633/1‑150 (MQ=255)
                             attattTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCa                                                                                                                          >  1:490898/1‑150 (MQ=255)
                                attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGCCCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                       >  2:525138/1‑150 (MQ=255)
                                attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                       <  2:237897/150‑1 (MQ=255)
                                attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                       <  2:1630647/150‑1 (MQ=255)
                                attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                       >  2:844673/1‑150 (MQ=255)
                                attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                       <  1:1460794/150‑1 (MQ=255)
                                attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCagag                                                                                                                       >  1:153222/1‑150 (MQ=255)
                                attTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCAGTCGCGTCTTATCAGACCTGGAGCAATTGACAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCTGCATTTGCTTAGCTCagag                                                                                                                       >  2:1392191/1‑150 (MQ=255)
                                       cTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGAt                                                                                                                >  1:1316667/1‑150 (MQ=255)
                                       cTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTGCTTAGCTCAGAGTGAAGAt                                                                                                                >  2:339233/1‑150 (MQ=255)
                                        tGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCAGGCGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCTGCATTTGCTTAGCTCAGAGTGAAGAtt                                                                                                               >  2:1113630/1‑150 (MQ=255)
                                          gCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTaa                                                                                                             >  2:1476625/1‑150 (MQ=255)
                                           tttctCAGTCCGAGTGTAGGCTGCATACGGTAATCAATGCCTTATGCGACGCTGTCGAGTCTTATCAGACATGGAGCAATTTCCAAATGTAGGCCTGATAAGGCGTTTACGCCGCATCAGGCATTTGCTTAGCTCAGAGTGAAGATTAAc                                                                                                            <  1:1113699/146‑1 (MQ=38)
                                            ttAGCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTaagc                                                                                                           <  2:1158950/150‑3 (MQ=255)
                                                 aGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCt                                                                                                      <  2:153222/150‑1 (MQ=255)
                                                  gTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCAACCGGCATTTGCTTAGCTCAGAGTGAAGATTAAACTATCTg                                                                                                     >  1:1576817/1‑150 (MQ=255)
                                                    ccGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGcc                                                                                                   <  2:837709/150‑1 (MQ=255)
                                                         tgtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTc                                                                                              >  2:633514/1‑150 (MQ=255)
                                                         tgtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTc                                                                                              >  2:1599077/1‑150 (MQ=255)
                                                          gtAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTcc                                                                                             >  1:435050/1‑150 (MQ=255)
                                                           tatgCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTccc                                                                                            <  2:124558/147‑1 (MQ=255)
                                                            aGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGCGAAGATTAACCTATCTGCCCATTcccc                                                                                           >  1:1727341/1‑150 (MQ=255)
                                                               cTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCCCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTACCCaaa                                                                                        >  1:405909/1‑150 (MQ=255)
                                                                 gCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATg                                                                                      >  2:551110/1‑150 (MQ=255)
                                                                 gCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCAGCCGGCATTTGCTTAGCTCAGCGTGAAGATTAACCTATCTGCCCATTCCCCAAATg                                                                                      >  2:1555215/1‑150 (MQ=255)
                                                                    tCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGttt                                                                                   <  2:687855/150‑1 (MQ=255)
                                                                      cGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTaa                                                                                 >  1:1173881/1‑150 (MQ=255)
                                                                       ggTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAAc                                                                                <  1:636432/150‑1 (MQ=255)
                                                                         tAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAAcgc                                                                              <  2:1526336/150‑1 (MQ=255)
                                                                              aaTGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGATGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCTgcgc                                                                         <  2:1074048/150‑1 (MQ=255)
                                                                                 gCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTa                                                                      <  2:26973/150‑1 (MQ=255)
                                                                                    tGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTTAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAAct                                                                   <  1:1381354/150‑1 (MQ=255)
                                                                                       tGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAActtct                                                                >  2:762381/1‑150 (MQ=255)
                                                                                       tGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAActtct                                                                >  2:448071/1‑150 (MQ=255)
                                                                                          gACGCTGTCGCGTCTTATCAGACCTGGAGCCATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCCTTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTcc                                                             <  1:919724/150‑1 (MQ=255)
                                                                                          gACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTcc                                                             <  1:633514/150‑1 (MQ=255)
                                                                                              cTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCt                                                         >  2:41169/1‑150 (MQ=255)
                                                                                                  cgcgTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATAAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTAcc                                                     >  1:219796/1‑150 (MQ=255)
                                                                                                     gTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGc                                                  <  1:542191/150‑1 (MQ=255)
                                                                                                       cTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACAAGCGt                                                >  1:1516749/1‑150 (MQ=255)
                                                                                                        ttATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGCGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGtt                                               <  2:1727341/150‑1 (MQ=255)
                                                                                                         tATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTCGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTa                                              <  1:1175730/150‑1 (MQ=255)
                                                                                                         tATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTa                                              >  1:1665937/1‑150 (MQ=255)
                                                                                                          aTCAGACATGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTaa                                             <  1:386099/150‑1 (MQ=255)
                                                                                                            cAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAAc                                           <  1:983557/150‑1 (MQ=255)
                                                                                                             aGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAAc                                                                    <  1:118830/124‑1 (MQ=255)
                                                                                                             aGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAAc                                                                    >  2:118830/1‑124 (MQ=255)
                                                                                                             aGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACg                                          <  1:1200924/150‑1 (MQ=255)
                                                                                                               cccTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTCAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGa                                        <  1:857072/149‑1 (MQ=255)
                                                                                                                 cTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACg                                      <  2:488955/150‑1 (MQ=255)
                                                                                                                  tGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGt                                     <  1:383584/150‑1 (MQ=255)
                                                                                                                  tGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGt                                     >  2:526691/1‑150 (MQ=255)
                                                                                                                   ggAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCATATTGAATATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTg                                    <  1:282060/150‑1 (MQ=255)
                                                                                                                   ggAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTg                                    <  2:221974/150‑1 (MQ=255)
                                                                                                                   ggAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTg                                    <  2:1189633/150‑1 (MQ=255)
                                                                                                                      gCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCAt                                 >  1:1184343/1‑150 (MQ=255)
                                                                                                                        aaTTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATcc                               >  1:540524/1‑150 (MQ=255)
                                                                                                                         aTTGCCAAATGTAGGCTGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCgg                             <  1:1320041/150‑1 (MQ=255)
                                                                                                                           tGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCggg                            >  1:308327/1‑150 (MQ=255)
                                                                                                                            gCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGGGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGGCGTGCATCCGGGc                           >  1:150515/1‑150 (MQ=255)
                                                                                                                             ccAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCt                          <  1:947751/150‑1 (MQ=255)
                                                                                                                             ccAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCt                          >  2:562563/1‑150 (MQ=255)
                                                                                                                              cAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTa                         >  2:1010071/1‑150 (MQ=255)
                                                                                                                                 aTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCt                      >  1:982734/1‑150 (MQ=255)
                                                                                                                                   aaatgCCGGATGCGGCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTaa                    >  1:1428695/5‑150 (MQ=255)
                                                                                                                                    tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTaaa                   <  1:1620697/150‑1 (MQ=255)
                                                                                                                                     aGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAat                  >  2:38323/1‑150 (MQ=255)
                                                                                                                                       gCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAatat                <  2:1699125/150‑1 (MQ=255)
                                                                                                                                       gCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAatat                >  1:504720/1‑150 (MQ=255)
                                                                                                                                           gATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGc            >  1:733675/1‑150 (MQ=255)
                                                                                                                                             tgcggCGTAAACGCCTTATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTTCCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGcac          >  1:806142/4‑150 (MQ=255)
                                                                                                                                              aaGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGcaca         <  2:490898/150‑1 (MQ=255)
                                                                                                                                                ggCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATc               >  2:1327121/1‑142 (MQ=255)
                                                                                                                                                ggCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACAcg       >  1:931938/1‑150 (MQ=255)
                                                                                                                                                   gTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTCCCTAAATAGCCGCACACgcgg    >  2:1272672/1‑150 (MQ=255)
                                                                                                                                                    tttACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACgcggc   <  1:1730996/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTCGACAGGATAGTACTGATGATAATTCATTATTTCGCCTGGCTTATCAGTCCGCGTGTAGGCTGCATCCGGTAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGACCTGGAGCAATTGCCAAATGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTGCTTAGCTCAGAGTGAAGATTAACCTATCTGCCCATTCCCCAAATGTTTAACGCGGCGCTTAACTTCTTCCTGCTTACCAGCGTTAAACGGACGTGCATCCGGGCTACCTAAATATCCGCACACGCGGCG  >  NC_000913/4460334‑4460632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: