BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,469,457	C→T	8.1%	T611T (ACC→ACT) ‡	mgtA →	magnesium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,469,457	0	C	T	8.1%	335.1 / 12.6	111	T611T (ACC→ACT) ‡	mgtA	magnesium transporter
Reads supporting (aligned to +/- strand): ref base C (50/52); new base T (4/5); total (54/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 113 total aligned reads displayed.

ACCGTAAAAATCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCTGTGGACGGCGC  >  NC_000913/4469314‑4469606

aCCGTAAAAATCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTg                                                                                                                                                 >  1:725840/1‑150 (MQ=255)

 ccGTAAAAATCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTTCCATGCAGTGGGGCTGGACGCGGGAGAGGTGGTCAATGGTAGTGATATAGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGt                                                                                                                                                >  1:1651622/1‑150 (MQ=255)

  cGTAAAAATCCTTCCTGGCGACCGTGAGTTATTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGTGAGATGGTGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTGGTACGCTGtg                                                                                                                                               <  2:297144/150‑2 (MQ=255)

   gTAATAATCCTTACTGGCGACAGTGAGTTAGTTGCTGCGAATGTGTGCCATGAAGTGTGGCTGGACGCGGGTGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAATTCTTTCACAGCGTACCACGCTGttt                                                                                                                                              <  2:1651809/150‑1 (MQ=255)

   gTAAAAATCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGttt                                                                                                                                              >  1:44624/1‑150 (MQ=255)

   gTAAAAATCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGttt                                                                                                                                              >  1:1344634/1‑150 (MQ=255)

    tAAAAATCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTGGTACGCTGtgca                                                                                                                                             <  1:1085088/150‑4 (MQ=255)

    tAAAAATCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTg                                                                                                                                             <  2:1495718/150‑1 (MQ=255)

     aaaaaTCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTGGTACGCTGtgcaa                                                                                                                                            <  1:1682472/150‑5 (MQ=255)

      aaaaTCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTGGTACGCTGtgcaag                                                                                                                                           <  2:1268116/150‑6 (MQ=255)

          tCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGcc                                                                                                                                       <  1:1689270/150‑1 (MQ=255)

           ccTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCt                                                                                                                                      <  2:949234/150‑1 (MQ=255)

            cTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTg                                                                                                                                     <  2:364119/150‑1 (MQ=255)

            cTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTg                                                                                                                                     <  2:1584517/150‑1 (MQ=255)

              tACTGGCGACAGTGTGTTAGTTGCAGCGAAAGTGTGCCATGTAGTGGGGCTGGACGCGTTAGAGGTGGTCATTGGTAGTGATTTTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGAc                                                                                                                                   <  2:408678/150‑1 (MQ=255)

               aCTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACg                                                                                                                                  <  1:1534511/150‑1 (MQ=255)

                cTGTCGACAGTGAGTTTGAGGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGTGAGTGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAAGCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGc                                                                                                                                 <  1:257870/150‑1 (MQ=255)

                 tGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGcc                                                                                                                                <  2:1003165/150‑1 (MQ=255)

                 tGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGcc                                                                                                                                <  2:1312206/150‑1 (MQ=255)

                  ggCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGccgccg                                                                                                                               >  1:1159652/1‑150 (MQ=255)

                  ggCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCg                                                                                                                               >  1:1501935/1‑150 (MQ=255)

                     gACCGTGAGTTAGTTGCAGCGAAAGTGTGCCTTGAATGGGGGCTGGACGCGGGAGAGGTGGTCATCGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATg                                                                                                                            <  1:1275726/150‑1 (MQ=255)

                     gACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACTAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCAGATg                                                                                                                            >  2:1220223/1‑150 (MQ=255)

                        aGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGCACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCAt                                                                                                                         <  2:919538/150‑1 (MQ=255)

                         gTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATa                                                                                                                        >  1:587006/1‑150 (MQ=255)

                         gTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATa                                                                                                                        >  2:1671540/1‑150 (MQ=255)

                          tGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATaa                                                                                                                       <  2:1117582/150‑1 (MQ=255)

                                   tGCAGCGAACGTGTGACATGAAGGGCGGCTGGACGCGGGAGGGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCAt                                                                                                              <  1:1485449/150‑1 (MQ=255)

                                     cAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGCGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATtg                                                                                                            <  2:379040/150‑1 (MQ=255)

                                      aGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATtgt                                                                                                           >  2:1294655/1‑150 (MQ=255)

                                      aGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATtgt                                                                                                           >  1:1189987/1‑150 (MQ=255)

                                         gAAAGTGTGACATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCAATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGAc                                                                                                        <  2:328633/150‑1 (MQ=255)

                                          aaaGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACTCCGATGCATAAAGAACGCATTGTGAcc                                                                                                       >  2:585724/1‑150 (MQ=255)

                                          aaaGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGAcc                                                                                                       >  2:955849/1‑150 (MQ=255)

                                            actgtgCCATGAATTGGGGCTGGACTCGGCAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCtt                                                                                                     <  2:846614/148‑1 (MQ=255)

                                               gtgCCATGAAGTGGGGCTGGACGAGGGAGAGTTTGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACt                                                                                                  <  2:1608475/150‑1 (MQ=255)

                                                 gCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACTCATTGTGACCTTACTGa                                                                                                >  2:1198441/1‑150 (MQ=255)

                                                   cATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAg                                                                                              <  1:968822/150‑1 (MQ=255)

                                                    aTGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGCCGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAgc                                                                                             >  2:851252/1‑150 (MQ=255)

                                                    aTGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAgc                                                                                             <  2:892309/150‑1 (MQ=255)

                                                          tGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAg                                                                                       <  1:240/150‑1 (MQ=255)

                                                            gggCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAggg                                                                                     >  1:797663/1‑150 (MQ=255)

                                                            gggCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAggg                                                                                     <  1:2460/150‑1 (MQ=255)

                                                                     gcgGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGtt                                                                            <  2:1507995/150‑1 (MQ=255)

                                                                     gcgGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATATTGCACAGCGTACCACGCTGTTTGCCCGGCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGtt                                                                            <  2:1248365/150‑1 (MQ=255)

                                                                           gagGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCCCGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCttt                                                                      <  2:1501935/150‑1 (MQ=255)

                                                                           gagGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCttt                                                                      <  2:788358/150‑1 (MQ=255)

                                                                           gagGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCttt                                                                      <  1:1181122/150‑1 (MQ=255)

                                                                             ggtggtCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTAt                                                                    >  1:103368/1‑150 (MQ=255)

                                                                              gtggtCATTGGTAGTGATATTTAAACTCTATCTGACGAAGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATg                                                                   <  2:1629847/150‑1 (MQ=255)

                                                                               tggtCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATgg                                                                  >  2:1348838/1‑150 (MQ=255)

                                                                                ggtCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATggg                                                                 <  2:1272320/150‑1 (MQ=255)

                                                                                ggtCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATggg                                                                 <  1:1618257/150‑1 (MQ=255)

                                                                                gggctatGGAAGTGAAATTGAAAAGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATggg                                                                 <  1:1328933/144‑1 (MQ=255)

                                                                                 gtCGTTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGc                                                                >  2:1719738/1‑150 (MQ=255)

                                                                                 gtCATTGGTAGTTATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGc                                                                <  2:464261/150‑1 (MQ=255)

                                                                                 gtCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCTTTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGc                                                                <  2:598269/150‑1 (MQ=255)

                                                                                 gtCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGc                                                                >  1:1164970/1‑150 (MQ=255)

                                                                                  tCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCg                                                               >  2:774788/1‑150 (MQ=255)

                                                                                   cATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGgaga                                                              >  2:384237/1‑150 (MQ=255)

                                                                                    aTTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGAt                                                             >  2:1260539/1‑150 (MQ=255)

                                                                                     ttGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATg                                                            <  2:747420/150‑1 (MQ=255)

                                                                                      tGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATgg                                                           >  1:670925/1‑150 (MQ=255)

                                                                                        gTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTa                                                         <  1:774788/150‑1 (MQ=255)

                                                                                            tGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTaa                                                     >  1:229383/1‑150 (MQ=255)

                                                                                             gATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAat                                                    >  2:1131599/1‑150 (MQ=255)

                                                                                               tatTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATg                                                            >  1:1609402/1‑140 (MQ=255)

                                                                                               tatTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATg                                                            <  2:1609402/140‑1 (MQ=255)

                                                                                               tatTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAatga                                                  <  1:1726555/150‑1 (MQ=255)

                                                                                                atTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAatgat                                                 >  2:49102/1‑150 (MQ=255)

                                                                                                atTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAatgat                                                 >  1:557657/1‑150 (MQ=255)

                                                                                                   gAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATgcg                                              <  2:805660/150‑1 (MQ=255)

                                                                                                       cGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGACGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGcgcccg                                          <  1:391015/150‑1 (MQ=255)

                                                                                                         cTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGcgcccgcc                                        >  1:96117/1‑150 (MQ=255)

                                                                                                          ggaatGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCt                                       >  1:1036958/5‑150 (MQ=255)

                                                                                                              tGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTAcg                                   >  2:533577/1‑150 (MQ=255)

                                                                                                                 cgacgaACTGGCAAATCTAGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACgcgc                                >  1:1284191/1‑150 (MQ=255)

                                                                                                                 cgacgaACTCGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTGGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACgcgc                                <  1:996201/150‑1 (MQ=255)

                                                                                                                     gcagtGGCAAATCTTTCACAGCGGACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACTCATTTTTACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCg                            <  2:1557525/146‑1 (MQ=255)

                                                                                                                         tGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGata                        >  1:1652346/1‑150 (MQ=255)

                                                                                                                          ggCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGatat                       >  1:1183817/1‑150 (MQ=255)

                                                                                                                               aTCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCa                  >  1:539084/1‑150 (MQ=255)

                                                                                                                               aTCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCa                  >  1:1730533/1‑150 (MQ=255)

                                                                                                                                  gcaaacaGCGTGGTACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCAttt               >  2:564343/5‑150 (MQ=255)

                                                                                                                                   tGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTc              >  2:1030641/1‑150 (MQ=255)

                                                                                                                                   caaacaGCGTGGTACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCAtttg              >  1:755226/4‑149 (MQ=255)

                                                                                                                                   caaacaGCGTGGTACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTc              >  2:1375585/4‑150 (MQ=255)

                                                                                                                                    aaacaGCGTGGTACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCt             >  2:1410612/3‑150 (MQ=255)

                                                                                                                                     cacaGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCtg            >  1:1713877/1‑150 (MQ=255)

                                                                                                                                     cacaGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCtg            >  1:811477/1‑150 (MQ=255)

                                                                                                                                     cacaGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTCACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCtg            <  2:887443/150‑1 (MQ=255)

                                                                                                                                      acaGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCtgt           >  1:1645300/1‑150 (MQ=255)

                                                                                                                                       caGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCtgtg          <  2:1354630/150‑1 (MQ=255)

                                                                                                                                       caGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCtgtg          >  1:507903/1‑150 (MQ=255)

                                                                                                                                       caGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACAGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATACGCCCGCCTTACGCGCTGCGGATATCGGCATTTCtgtg          >  1:1379866/1‑150 (MQ=255)

                                                                                                                                        aGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGACCGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCTGTgg         <  1:853991/150‑1 (MQ=255)

                                                                                                                                        aGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCTGTgg         <  1:1671540/150‑1 (MQ=255)

                                                                                                                                             aCCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCTGTGGACGgc    <  1:533577/150‑1 (MQ=255)

                                                                                                                                               cACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCTGTGGACGgcgc  >  1:436713/1‑150 (MQ=255)

                                                                                                                                               cACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCTGTGGACGgcgc  >  1:681138/1‑150 (MQ=255)

ACCGTAAAAATCCTTACTGGCGACAGTGAGTTAGTTGCAGCGAAAGTGTGCCATGAAGTGGGGCTGGACGCGGGAGAGGTGGTCATTGGTAGTGATATTGAAACGCTATCTGACGACGAACTGGCAAATCTTGCACAGCGTACCACGCTGTTTGCCCGCCTGACGCCGATGCATAAAGAACGCATTGTGACCTTACTGAAGCGCGAAGGGCATGTGGTTGGCTTTATGGGCGATGGTATTAATGATGCGCCCGCCTTACGCGCTGCGGATATCGGCATTTCTGTGGACGGCGC  >  NC_000913/4469314‑4469606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`