Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,470,951 A→C 8.1% intergenic (‑38/+35) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,470,9510AC 8.1% 374.2 / 17.4 133intergenic (‑38/+35)ridA/pyrIenamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
Reads supporting (aligned to +/- strand):  ref base A (62/60);  new base C (6/5);  total (68/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 137 total aligned reads displayed.

TCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGG  >  NC_000913/4470802‑4471100
                                                                                                                                                        |                                                                                                                                                     
tCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCa                                                                                                                                                       >  2:381577/1‑150 (MQ=255)
tCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGATCa                                                                                                                                                       >  1:935170/1‑150 (MQ=255)
    aTTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATtat                                                                                                                                                   <  2:395415/150‑1 (MQ=255)
       tACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAgg                                                                                                                                                >  1:1478491/1‑150 (MQ=255)
       tACCGGGATCTGACAGGAGGTGATGATCATATTGCCCTGATCAACGCCCTGTACGTAAGGACCGAAAGCTGCCGGTGCATTTTCCTTCGAGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAgg                                                                                                                                                <  2:894688/150‑1 (MQ=255)
        aCCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAggg                                                                                                                                               >  1:1635021/1‑150 (MQ=255)
         ccGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGa                                                                                                                                              >  2:3446/1‑150 (MQ=255)
          cGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAg                                                                                                                                             >  2:377175/1‑150 (MQ=255)
               tCTGACAGGAGGTGATGATCATATTGCCCAGATAAACTCCCTGTACGTACTGAGCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTAATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGGTCCCTATAATGAGCCAGa                                                                                                                                        <  2:1006818/150‑1 (MQ=255)
                cTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGAc                                                                                                                                       <  2:381393/150‑1 (MQ=255)
                cTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGAc                                                                                                                                       <  1:1729855/150‑1 (MQ=255)
                 tGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCCCTATAATGAGCCAGACt                                                                                                                                      <  1:770954/150‑1 (MQ=255)
                 tGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACt                                                                                                                                      >  1:127663/1‑150 (MQ=255)
                 tGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACt                                                                                                                                      >  1:132944/1‑150 (MQ=255)
                  gACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCCCTATAATGAGCCAGACtt                                                                                                                                     <  2:703943/150‑1 (MQ=255)
                  gACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACtt                                                                                                                                     <  2:1421737/150‑1 (MQ=255)
                  gACCGGAGGTGATGATCATATTGCCCAGATCAACGACCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATATTTTTGCTCATGATTTCTCCTTTATTACATCGGTA‑‑AAAAGTCTGGATCATTATAGGGAGCCAGACtt                                                                                                                                     >  1:1672164/1‑150 (MQ=255)
                    ccGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTAATAAAAGTCTGGCTCCCTATAATGAGCCAGACtt                                                                                                                                     <  1:815744/150‑1 (MQ=38)
                     cGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTtta                                                                                                                                  <  1:526206/150‑1 (MQ=255)
                       gAGGTGATGATCATATTGCCCATATCAACGCCCTGTTAGTAATGACCTATAGCTGCCGGTGAATTTTACGTCGCTATAGTTTTGATCATGATTTCTCCTTTATTACAGCTGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTttatt                                                                                                                                <  2:324295/150‑1 (MQ=255)
                        aGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTttatta                                                                                                                               >  1:230038/1‑150 (MQ=255)
                          gTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAcc                                                                                                                             <  1:558514/150‑1 (MQ=255)
                          gTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTAcc                                                                                                                             <  2:1329715/150‑1 (MQ=255)
                           tgatgaTCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCa                                                                                                                            >  2:1485087/1‑150 (MQ=255)
                           tgatgaTCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCa                                                                                                                            <  1:1348910/150‑1 (MQ=255)
                              tgatCATATTGCCCAGATCAAGTCCGTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTAGCGATAGTTTTGCTAATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCGTCATTATAGGGAGCCAGACTTTTATTACCAAc                                                                                                                          <  2:487854/150‑1 (MQ=255)
                              tgatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTCTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAAcc                                                                                                                         >  2:1593386/1‑150 (MQ=255)
                               gatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCg                                                                                                                        >  1:686341/1‑150 (MQ=255)
                               gatCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCg                                                                                                                        >  1:552653/1‑150 (MQ=255)
                                ttCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGc                                                                                                                       <  2:947816/149‑1 (MQ=255)
                                 tCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCa                                                                                                                      >  1:1254850/1‑150 (MQ=255)
                                  cATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTTTTACCAACCGCaa                                                                                                                     >  2:886346/1‑150 (MQ=255)
                                    tatTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAACGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaatt                                                                                                                   >  1:753231/1‑150 (MQ=255)
                                        gCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCaattaatt                                                                                                               >  1:472612/1‑150 (MQ=255)
                                          ccAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTgg                                                                                                             >  2:1190137/1‑150 (MQ=255)
                                            aGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGcc                                                                                                           <  1:20802/150‑1 (MQ=255)
                                            aGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGcc                                                                                                           <  1:958919/150‑1 (MQ=255)
                                               tCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGc                                                                                                        >  2:647644/1‑150 (MQ=255)
                                                cAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGca                                                                                                       <  1:920057/150‑1 (MQ=255)
                                                 aaCGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcac                                                                                                      >  2:1588517/1‑150 (MQ=255)
                                                    gCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAATCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGcaccac                                                                                                   <  1:1573834/150‑1 (MQ=255)
                                                     cccTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACcaca                                                                                                  >  2:810191/1‑150 (MQ=255)
                                                         gTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACAACATTAt                                                                                              >  1:1292758/1‑150 (MQ=255)
                                                          ttagTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTATATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATg                                                                                             <  1:659701/147‑1 (MQ=255)
                                                          tACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATg                                                                                             <  2:230038/150‑1 (MQ=255)
                                                           aCGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATgg                                                                                            <  1:1232873/150‑1 (MQ=255)
                                                               aaGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaaa                                                                                        >  1:731235/1‑150 (MQ=255)
                                                               aaGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGaaa                                                                                        >  2:718971/1‑150 (MQ=255)
                                                                 ggACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAAc                                                                                      >  2:1389199/1‑150 (MQ=255)
                                                                   aCCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAActc                                                                                    >  2:1094192/1‑150 (MQ=255)
                                                                         tgCTGCCGGGGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTc                                                                              <  2:79364/149‑1 (MQ=255)
                                                                          gCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTca                                                                             <  2:1635021/150‑1 (MQ=255)
                                                                           cTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTcac                                                                            >  2:880624/1‑150 (MQ=255)
                                                                             gCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTATTTTTctcat                                                                          >  2:164570/1‑149 (MQ=255)
                                                                                 gTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTAtt                                                                      >  2:396081/1‑150 (MQ=255)
                                                                                       tttCCGTCTCGATAGTTTTGCTGATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCAtt                                                                <  1:687591/150‑1 (MQ=255)
                                                                                         tCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTg                                                              <  1:1630148/150‑1 (MQ=255)
                                                                                            gTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAgc                                                           <  2:1254850/150‑1 (MQ=255)
                                                                                               gcgATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGcgcg                                                        >  1:1684607/1‑150 (MQ=255)
                                                                                                 gATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACGTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAACCACTTTTTCACATTATTTGAATTTGAGCGCGat                                                      >  2:647694/1‑150 (MQ=255)
                                                                                                 gATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTAGAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGAACCACATTATGGGAAAACTCTTTTTAACAGTATTTGAATTTGAGCGCGat                                                      >  2:1500691/1‑150 (MQ=255)
                                                                                                        ttGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTg                                               <  2:1418539/150‑1 (MQ=255)
                                                                                                                gATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGttt                                       <  1:886346/150‑1 (MQ=255)
                                                                                                                   ttCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGAATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCg                                    <  2:748240/150‑1 (MQ=255)
                                                                                                                    tctcCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGc                                   >  2:1192043/1‑150 (MQ=255)
                                                                                                                    tctcCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGc                                   >  2:181360/1‑150 (MQ=255)
                                                                                                                    tctcCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGc                                   <  2:1346021/150‑1 (MQ=255)
                                                                                                                     ctcCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTTACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCa                                  >  1:825747/1‑150 (MQ=255)
                                                                                                                      tcCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGGTTTCGCAc                                 <  2:456372/150‑1 (MQ=255)
                                                                                                                       ccTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGATCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACg                                <  1:369358/150‑1 (MQ=255)
                                                                                                                        cTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACgg                               >  2:183702/1‑150 (MQ=255)
                                                                                                                         tttATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGc                              >  1:302692/1‑150 (MQ=255)
                                                                                                                           tattaCAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGAGCGATATCATTGGCGCGTTTTCGCACGGCaa                            >  1:1645325/1‑150 (MQ=255)
                                                                                                                            attaCAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTATTTGGCCAGCACCACATTATGGGAAAACTCTTTATCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaaa                           <  1:276887/150‑1 (MQ=255)
                                                                                                                            attaCAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCaaa                           >  1:379050/1‑150 (MQ=255)
                                                                                                                                   cGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGAt                    <  2:127663/150‑1 (MQ=255)
                                                                                                                                    ggTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATg                   >  2:349877/1‑150 (MQ=255)
                                                                                                                                           aaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAcc              >  2:1530645/1‑150 (MQ=255)
                                                                                                                                           aaaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAAcc              >  2:1315218/1‑150 (MQ=255)
                                                                                                                                            aaGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCg             >  1:1186017/1‑150 (MQ=255)
                                                                                                                                             aGTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCgg            >  1:55382/1‑150 (MQ=255)
                                                                                                                                             aGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCgg            >  1:108346/1‑150 (MQ=255)
                                                                                                                                             aGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCgg            >  2:408987/1‑150 (MQ=255)
                                                                                                                                              gTCTGGCTCCCTATAATGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGt           >  1:1731579/1‑150 (MQ=255)
                                                                                                                                              gTCTGGCTCCCTATAAGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTGTCGCACGGCAAAGCTGGATGAAACCGGt           >  1:1429967/1‑150 (MQ=255)
                                                                                                                                              gTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGt           >  1:1081600/1‑150 (MQ=255)
                                                                                                                                              gTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGt           <  1:1389199/150‑1 (MQ=255)
                                                                                                                                                cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTc         >  1:1077253/1‑150 (MQ=255)
                                                                                                                                                cTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTc         <  1:183702/150‑1 (MQ=255)
                                                                                                                                                 tGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCg        <  1:1452621/150‑1 (MQ=255)
                                                                                                                                                  ggCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCgg       >  2:430413/1‑150 (MQ=255)
                                                                                                                                                   gCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGc      >  1:35675/1‑150 (MQ=255)
                                                                                                                                                    cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa     >  1:1690520/1‑150 (MQ=255)
                                                                                                                                                    cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa     >  1:1175743/1‑150 (MQ=255)
                                                                                                                                                    cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa     <  2:1478491/150‑1 (MQ=255)
                                                                                                                                                    cTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCa     >  1:1411707/1‑150 (MQ=255)
                                                                                                                                                       cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATg   <  1:1180407/150‑1 (MQ=255)
                                                                                                                                                       cATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATg   <  1:1729870/150‑1 (MQ=255)
                                                                                                                                                        aTTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATgg  >  1:12307/1‑150 (MQ=255)
                                                                                                                                                        aTTATAGGGAGCCAGACTTATATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATgg  <  2:935170/150‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
TCGGATTTACCGGGATCTGACCGGAGGTGATGATCATATTGCCCAGATCAACGCCCTGTACGTAAGGACCGATAGCTGCCGGTGCATTTTCCGTCGCGATAGTTTTGCTCATGATTTCTCCTTTATTACAGCGGTA‑‑AAAAGTCTGGCTCATTATAGGGAGCCAGACTTTTATTACCAACCGCAATTAATTGGCCAGCACCACATTATGGGAAAACTCTTTTTCACAGTATTTGCATTTGAGCGCGATATCATTGGCGCGTTTTCGCACGGCAAAGCTGGATGAAACCGGTTCGGCATGG  >  NC_000913/4470802‑4471100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: