BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,502,107	G→T	5.6%	A442D (GCT→GAT) ‡	insG ←	IS4 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,502,107	0	G	T	5.6%	369.6 / 7.7	124	A442D (GCT→GAT) ‡	insG	IS4 transposase
Reads supporting (aligned to +/- strand): ref base G (62/54); new base T (4/3); total (67/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 126 total aligned reads displayed.

GCATGACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGT  >  NC_000913/4501958‑4502256

gCATGACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAg                                                                                                                                                       <  2:649004/150‑1 (MQ=255)

 cATGACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGc                                                                                                                                                      <  1:662519/150‑1 (MQ=255)

    gACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAac                                                                                                                                                   >  1:768432/1‑150 (MQ=255)

      cAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAaatg                                                                                                                                                 >  1:1471727/1‑150 (MQ=255)

        aTGTCAAACAGTCAAAGATTGTGGCGTGGACGGTCAGTCTTGGGGACAAAACAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAactgac                                                                                                                                               <  2:1240632/150‑1 (MQ=255)

          gTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAactgactg                                                                                                                                             <  1:96119/150‑1 (MQ=255)

          gTCAAACAGTCAAAGATTGTGGCGTTGAATTTCAGTCTTGGGGACAAAAAAGATCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGATGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAactgactg                                                                                                                                             <  1:83233/150‑1 (MQ=255)

           tCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGTGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTGCTTAACTGACTgg                                                                                                                                            <  1:1161016/150‑1 (MQ=255)

           tCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTGCTTAACTGACTgg                                                                                                                                            <  2:688265/150‑1 (MQ=255)

           tCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTgg                                                                                                                                            <  1:724244/150‑1 (MQ=255)

            cAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGc                                                                                                                                           <  2:1557402/150‑1 (MQ=255)

              aaCAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGctc                                                                                                                                         >  1:116952/1‑150 (MQ=255)

                     aaaGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCg                                                                                                                                  >  1:1308464/1‑150 (MQ=255)

                     aaaGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCg                                                                                                                                  >  2:483501/1‑150 (MQ=255)

                      aaGATTGTGGCGTTGACGGTCAGTCTTGGGGAC‑AAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCggg                                                                                                                                <  2:940436/150‑1 (MQ=255)

                        taTTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCgggg                                                                                                                               <  1:181865/149‑1 (MQ=255)

                        gATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCgggg                                                                                                                               <  2:1332826/150‑1 (MQ=255)

                         atggtgGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGc                                                                                                                              <  1:6691/147‑1 (MQ=255)

                         aTTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGc                                                                                                                              <  2:924082/150‑1 (MQ=255)

                              ggCGTTGACGGTCAGTCTTGTGTACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTgg                                                                                                                         <  2:1720717/150‑1 (MQ=255)

                               gCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTggg                                                                                                                        <  1:1539913/150‑1 (MQ=255)

                               gCGTTGACGGGCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTggg                                                                                                                        <  2:233402/150‑1 (MQ=255)

                                cGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTgggg                                                                                                                       <  2:613965/150‑1 (MQ=255)

                                   tGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTAt                                                                                                                    >  1:1049802/1‑150 (MQ=255)

                                   tGACGATCAGTCTTGTGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGAAGCTGAAAAGAAAAACGCATCTTGGTTCTGTAATGCCAGTCAGTTTAGCAACTGACTGGCTAGTATTCGGGGGTGTGGGGTAt                                                                                                                    >  2:1658579/1‑150 (MQ=255)

                                    gACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTAtt                                                                                                                   >  1:548435/1‑150 (MQ=255)

                                      cGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTc                                                                                                                 >  2:1440203/1‑150 (MQ=255)

                                         tCAGTCTTGGGGAC‑AAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAgg                                                                                                             <  1:49837/150‑1 (MQ=255)

                                          cAGGCTTGGGGACAAAAAAGCACAGTATTCTCGTTGGCAACCAGATAATTTAACAGATGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAgg                                                                                                             <  2:350560/150‑1 (MQ=255)

                                            gTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGc                                                                                                           >  2:1007262/1‑150 (MQ=255)

                                            gTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGc                                                                                                           >  1:1136519/1‑150 (MQ=255)

                                             tCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGcc                                                                                                          <  1:378112/150‑1 (MQ=255)

                                             tCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATATAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGcc                                                                                                          <  1:1267920/150‑1 (MQ=255)

                                              cTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTGCTTAACTGACTGGc                                                                                                                                           <  1:483808/116‑1 (MQ=255)

                                              cTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTGCTTAACTGACTGGc                                                                                                                                           >  2:483808/1‑116 (MQ=255)

                                                tGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCctct                                                                                                       <  2:7062/150‑1 (MQ=255)

                                                 ggggACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCtctc                                                                                                      >  1:1332919/1‑150 (MQ=255)

                                                 ggggACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCtctc                                                                                                      >  1:325239/1‑150 (MQ=255)

                                                       aaaaaaGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAATTAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTAc                                                                                                <  2:949162/150‑1 (MQ=255)

                                                        aaaaaGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTAcc                                                                                               <  2:868314/150‑1 (MQ=255)

                                                            aGCTCAGTAATCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGAATGGCTCTTTTTCCGGGCTGTGGGGTATTTCCAGGGCCTCTCATGTACCActc                                                                                           >  2:1591130/1‑150 (MQ=255)

                                                                    aTTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAg                                                                                   <  1:837285/150‑1 (MQ=255)

                                                                    aTTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAg                                                                                   >  2:1455309/1‑150 (MQ=255)

                                                                    aTTCTCGTAGGCAACCAGATTATTTAACAGTTGATCCTGAAACGAAATAGGCATCTTTGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCAATCTCCGGAAg                                                                                   >  2:352874/1‑150 (MQ=255)

                                                                     ttCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAgg                                                                                  <  2:1433413/150‑1 (MQ=255)

                                                                     ttCTCGTTCGCAACCAGATAATTTCACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAgg                                                                                  <  1:270707/150‑1 (MQ=255)

                                                                        tcGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGccc                                                                               <  2:1108540/150‑1 (MQ=255)

                                                                         cGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGTCCCt                                                                              >  2:1629021/1‑150 (MQ=255)

                                                                         cGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCt                                                                              >  2:1512991/1‑150 (MQ=255)

                                                                         cGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCt                                                                              >  2:1506321/1‑150 (MQ=255)

                                                                          gTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGTGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCACtt                                                                             >  1:208186/1‑150 (MQ=255)

                                                                            tGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTc                                                                           >  1:182874/1‑150 (MQ=255)

                                                                             ggCAACCAGATTATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTATGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGCGTATTTCCAGAGCCTCTACTTTACCACTCTCGGGAAGGCCCTTTcc                                                                          >  2:702833/1‑150 (MQ=255)

                                                                             ggCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTcc                                                                          >  2:24799/1‑150 (MQ=255)

                                                                              gCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTGGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCGTTGCCACTCTCGGGAAGGCCCTTTccc                                                                         >  2:381490/1‑150 (MQ=255)

                                                                               cAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGCCTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCATCTCCTTTACCACTCTCGGGAAGGCCCTTTCCct                                                                        >  2:856312/1‑150 (MQ=255)

                                                                                 aCCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGTGAAGGCCCTTTCCcttg                                                                      >  1:1064769/1‑149 (MQ=255)

                                                                                   cAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCcttctt                                                                    <  1:1653196/150‑1 (MQ=255)

                                                                                    aGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTCAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTg                                                                   <  1:266588/150‑1 (MQ=255)

                                                                                    aGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTg                                                                   >  2:44578/1‑150 (MQ=255)

                                                                                     gATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAggggc                                                                                                          <  1:680183/110‑3 (MQ=255)

                                                                                       tAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCg                                                                <  2:1136519/150‑1 (MQ=255)

                                                                                       tAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGATAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCATTCTTGTCg                                                                >  2:1679945/1‑150 (MQ=255)

                                                                                         aTTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGt                                                              >  1:549371/1‑150 (MQ=255)

                                                                                         aTTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGGATTTCCAGGGCCGCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGt                                                              <  1:290779/150‑1 (MQ=255)

                                                                                             aaCAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGTCCCTTTCCCTTCTTGTCGGTAAtt                                                          >  1:1154648/1‑150 (MQ=255)

                                                                                             aaCAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAAtt                                                          <  1:399727/150‑1 (MQ=255)

                                                                                               cAGTTGTTGCTGAAACTAATTAGGCATCTTGGTTCTGTTATGCCAGTCAGTTTAGCTACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTACTTTACCACTCTCGGGATGGCCCTTTCCCTTCTTGTCGGTAATTTc                                                        <  1:437018/150‑1 (MQ=255)

                                                                                                aGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTCCCACTCTCGGGAAGGCCCTTGCCCGTCTTGTCGGTAATTTca                                                       >  2:345521/1‑150 (MQ=255)

                                                                                                aGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTca                                                       >  2:316243/1‑150 (MQ=255)

                                                                                                     aTGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCTGTAATTTCACAAGt                                                  >  1:298919/1‑150 (MQ=255)

                                                                                                         tGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTc                                              >  2:521686/1‑150 (MQ=255)

                                                                                                          gAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTcc                                             <  1:346610/150‑1 (MQ=255)

                                                                                                           aaaCGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTccc                                            <  2:1049802/150‑1 (MQ=255)

                                                                                                            aaCGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCa                                           >  2:1371659/1‑150 (MQ=255)

                                                                                                                 aaTAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTATCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACtt                                      >  2:1100809/1‑150 (MQ=255)

                                                                                                                   tAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCGTGTCGGTTATTTCACAAGTTGTCCCATACTTGc                                    >  2:1360960/1‑150 (MQ=255)

                                                                                                                     ggCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCaa                                  >  1:1649002/1‑150 (MQ=255)

                                                                                                                         tCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATc                              <  1:1455309/150‑1 (MQ=255)

                                                                                                                          cTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATcg                             <  1:483501/150‑1 (MQ=255)

                                                                                                                          cTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCGTCTTGGCGGGATTTTCTCAACTTGTCGCATACTTGctcgctcc                             >  1:281378/1‑143 (MQ=255)

                                                                                                                                 cTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCa                      <  2:116952/150‑1 (MQ=255)

                                                                                                                                 cTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCa                      >  2:726525/1‑150 (MQ=255)

                                                                                                                                  tGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAg                     <  2:248371/150‑1 (MQ=255)

                                                                                                                                   gTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGc                    >  1:1588985/1‑150 (MQ=255)

                                                                                                                                   gTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGc                    <  1:24268/150‑1 (MQ=255)

                                                                                                                                     aaTGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTc                  >  1:519413/1‑150 (MQ=255)

                                                                                                                                      aTGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTcc                 <  2:1154648/150‑1 (MQ=255)

                                                                                                                                       tGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCg                >  1:1181961/1‑150 (MQ=255)

                                                                                                                                        gCCAGTCAGTTGCTTAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCgg               >  1:597118/1‑150 (MQ=255)

                                                                                                                                        gCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAAATCGCGCATCAGCTCCgg               >  2:1415127/1‑150 (MQ=255)

                                                                                                                                        gCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTAATTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCgg               <  1:1239687/150‑1 (MQ=255)

                                                                                                                                         ccAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGt              >  2:1119356/1‑150 (MQ=255)

                                                                                                                                         ccAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGGATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCggg              <  1:767926/150‑2 (MQ=255)

                                                                                                                                           agtcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGtat            >  2:602760/1‑150 (MQ=255)

                                                                                                                                           agtcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACAACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGtat            >  1:1053560/1‑150 (MQ=255)

                                                                                                                                            gtcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAATTTGTCCCATACTTGCAAGATCGAGCATCAGCTCCGGtata           >  2:1074693/1‑150 (MQ=255)

                                                                                                                                            gtcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGtata           >  2:773519/1‑150 (MQ=255)

                                                                                                                                             tcagtTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATAc          <  2:687493/150‑1 (MQ=255)

                                                                                                                                                     gCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCggg  >  2:137342/1‑149 (MQ=255)

GCATGACAATGTCAAACAGTCAAAGATTGTGGCGTTGACGGTCAGTCTTGGGGACAAAAAAGCTCAGTATTCTCGTTGGCAACCAGATAATTTAACAGTTGATGCTGAAACGAAATAGGCATCTTGGTTCTGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTTCCAGGGCCTCTCCTTTACCACTCTCGGGAAGGCCCTTTCCCTTCTTGTCGGTAATTTCACAAGTTGTCCCATACTTGCAAGATCGCGCATCAGCTCCGGTATACGTCCCGGT  >  NC_000913/4501958‑4502256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`