Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,519,313 C→A 5.1% intergenic (+89/+25) insB1 → / ← yjhU IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,519,3130CA 5.1% 365.0 / 4.8 129intergenic (+89/+25)insB1/yjhUIS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element
Reads supporting (aligned to +/- strand):  ref base C (64/56);  new base A (4/4);  total (69/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAG  >  NC_000913/4519166‑4519456
                                                                                                                                                   |                                                                                                                                               
cGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTa                                                                                                                                               >  2:150477/1‑150 (MQ=255)
        ttGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGc                                                                                                                                       <  1:1492070/150‑1 (MQ=255)
         tGCATGACAAGGTCATCGGGCATTATCTGACCATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAACATACTGCCCCATATCTACAGGGTGCa                                                                                                                                      >  2:1159053/1‑150 (MQ=37)
         tGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACCCTATCAGTAAGTTGGAGTCAGTACCGACCATGGTTATTTCATACATTGTGGGTATTGTTCTTATTATGGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCa                                                                                                                                      >  2:219655/1‑150 (MQ=255)
         tGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCa                                                                                                                                      >  1:431745/1‑150 (MQ=255)
          gCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTTTTTCTTACATTGTGGGTATTGTTCGTATTATCGGAGCTAATCAATAAAATCCTGCCGCATAGCTACTTGGGGGAg                                                                                                                                     >  2:292035/1‑150 (MQ=25)
          gCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATGTAGATATGGGGCAg                                                                                                                                     <  1:1723916/150‑1 (MQ=255)
          gCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAg                                                                                                                                     >  2:757559/1‑150 (MQ=255)
           cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgt                                                                                                                                    <  2:246980/150‑1 (MQ=255)
           cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgt                                                                                                                                    <  1:956261/150‑1 (MQ=255)
             tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATGTAGATATGGGGCAggat                                                                                                                                  <  2:447445/150‑4 (MQ=37)
             tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttg                                                                                                                                  >  2:1399795/1‑150 (MQ=255)
             tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttg                                                                                                                                  >  2:410050/1‑150 (MQ=255)
             tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttg                                                                                                                                  >  2:621390/1‑150 (MQ=255)
             tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttg                                                                                                                                  <  1:1086486/150‑1 (MQ=255)
               aCAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgtt                                                                                                                                <  2:997405/150‑1 (MQ=255)
               aCAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgtt                                                                                                                                <  2:92900/150‑1 (MQ=255)
                cAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTc                                                                                                                               >  2:235754/1‑150 (MQ=255)
                  atgTCATCGGGCATTATCTGAACATAAAACACTCTCAGTAAGTTGGAGTCAATACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAAACAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCAt                                                                                                                             <  1:1483498/148‑1 (MQ=255)
                  aGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATAGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGCTCAt                                                                                                                             >  2:391896/1‑150 (MQ=255)
                   ggTCATCGGGCATTATCTGAACATAAAACACTGTCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCAtt                                                                                                                            >  2:724832/1‑150 (MQ=255)
                   ggTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCAtt                                                                                                                            >  2:1388678/1‑150 (MQ=255)
                   ggTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCAtt                                                                                                                            >  2:1722878/1‑150 (MQ=255)
                       aTCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCttt                                                                                                                        <  2:28365/150‑1 (MQ=255)
                          gggCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGCCCATGTTTATTTCCTACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAg                                                                                                                     <  2:1594292/150‑1 (MQ=255)
                          gggCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAg                                                                                                                     <  2:541837/150‑1 (MQ=255)
                          gggCATTATCTGAACAAAAAACACTATCAGTAATTTGGAGTCATTACAGAACATGTTTATTTCATACAATGAGGGTATTGTTCTTATTATAGCCGCTATTCAATAAAATCCTGCAAAATATCTACATGGGGCAGTTGTTCATTCTTTTAg                                                                                                                     >  2:174400/1‑150 (MQ=255)
                             cATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCACCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtgt                                                                                                                  >  1:1243328/1‑150 (MQ=255)
                              aTTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGtgtg                                                                                                                 >  2:707117/1‑150 (MQ=255)
                               ttATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTgg                                                                                                                <  1:527180/150‑1 (MQ=255)
                                tATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGt                                                                                                               >  1:1175700/1‑150 (MQ=255)
                                   atGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTTTTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCACCATATATACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAt                                                                                                            <  2:156697/149‑1 (MQ=255)
                                    tGAACATAAAAAACTCTCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCCCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAtt                                                                                                           <  2:923731/150‑1 (MQ=255)
                                     gAACATATAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTAATTCTTTTAGTTTGGTAAtta                                                                                                          >  1:665710/1‑149 (MQ=255)
                                     gAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTc                                                                                                          >  2:1526961/1‑150 (MQ=255)
                                       aCATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTcac                                                                                                        >  1:687496/1‑150 (MQ=255)
                                         aTAAATCACTATCAGTAAGATGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCACATATCTACATGGGGCAGTTGTTCATTATTTTAGTGTGGTAATTCacac                                                                                                      >  1:347138/1‑150 (MQ=255)
                                         aTAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCacac                                                                                                      >  2:521606/1‑150 (MQ=255)
                                           aaaaCACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGAAATACACACGc                                                                                                    >  2:861148/1‑150 (MQ=255)
                                           aaaaCACTATAAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGAGACAGATCTACATTCTTTTATTCTAGTAATTCACACGc                                                                                                    >  2:858964/1‑150 (MQ=255)
                                            aaaCACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGcc                                                                                                   >  2:1308425/1‑150 (MQ=255)
                                             aaCACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCa                                                                                                  >  2:1547169/1‑150 (MQ=255)
                                               cacTATCAGTAAGTTGGAGTCCTTACCGCCCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCGAATCAATAAAATCCTGCCCCATATCTCCATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGc                                                                                                >  1:1653601/1‑150 (MQ=255)
                                                   aTCAGTAAGTTGGAGTCATTACCGACGATGTTTATTTCATACATTGTGGGTATTGTTCTTATTTTCGCCGCTAATCAATAAAATCCTGCCCCATATGTACAGGGGGCAGTTCTTCATTCTTTTAGTGTGGTATTTCACACGCCAGCaaaa                                                                                            >  2:292735/1‑150 (MQ=255)
                                                    tCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCaaaaa                                                                                           <  2:8389/150‑1 (MQ=255)
                                                     cAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAAc                                                                                          <  1:993399/150‑1 (MQ=255)
                                                         aaGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTg                                                                                      <  1:612765/150‑1 (MQ=255)
                                                           gTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGcc                                                                                    >  1:1465116/1‑150 (MQ=255)
                                                             tGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTAATTCTTTTATTGTGGTAATTCACACGCCAGCAAAAACTCAGCCGt                                                                                  >  1:57317/1‑150 (MQ=255)
                                                                   cATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGATCCTtc                                                                            >  2:1040568/1‑150 (MQ=255)
                                                                       aCCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcatca                                                                        <  1:621390/150‑1 (MQ=255)
                                                                        ccGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaa                                                                       <  2:150270/150‑1 (MQ=255)
                                                                         cGACCATGTTTATTTCATACATTGTGGGTTTTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaac                                                                      <  2:1499727/150‑1 (MQ=255)
                                                                         cGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCCTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaac                                                                      >  2:1393481/1‑150 (MQ=255)
                                                                               tGTTTATTTCATACATTTTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAg                                                                <  1:1322798/150‑1 (MQ=255)
                                                                                gTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGACGCTAATCAATAAAATCCTGCCCCATATCTACATTTGTAATTTTTTCATTATTTTTGTGTGTTAATTAACACTCAAACAAAAAATATGACGTTCCTTAATCAAAAATCAgt                                                               >  1:341316/1‑149 (MQ=255)
                                                                                   tATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTcc                                                            >  2:1030805/1‑150 (MQ=255)
                                                                                      ttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATTGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTg                                                         <  1:209712/150‑1 (MQ=255)
                                                                                      ttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTg                                                         <  1:707117/150‑1 (MQ=255)
                                                                                       tCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGa                                                        <  1:1041305/150‑1 (MQ=255)
                                                                                       tCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCAGCCGTACCTTCATCAACAATCAGGTCCGTGa                                                        >  2:1077255/1‑150 (MQ=255)
                                                                                        cATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGAc                                                       >  1:799517/1‑150 (MQ=255)
                                                                                        cATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCACCAAAAACTCTGCCGTTCCTTCATCATCAATCAGGTCCGTgcc                                                       >  2:111829/1‑148 (MQ=255)
                                                                                          tACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACTTGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACat                                                     >  1:1561858/1‑150 (MQ=255)
                                                                                              ttGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATAtcc                                                 <  1:757559/150‑1 (MQ=255)
                                                                                                gtgGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTTCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATAtcctc                                               <  2:798563/150‑1 (MQ=255)
                                                                                                gtgGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATAtcctc                                               >  2:479839/1‑150 (MQ=255)
                                                                                                      aTTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCCGTTGATCATTCTTTTAGTGTGTTTATTCAAACGCCCGCAAAAACTCTGCCGTTCCTTCAGCAACAATCAGGGCCGTGCAATATCATCCcagca                                         >  2:320699/1‑150 (MQ=255)
                                                                                                        tGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTTACATATCCTCCCAGCAgg                                       >  2:1727986/1‑150 (MQ=255)
                                                                                                         gTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCTGTTGTTCATTCTTTTAGTGTTGTAATTCACACGCCTGAAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAggg                                      <  1:1133074/150‑1 (MQ=255)
                                                                                                         gTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAggg                                      <  2:1175700/150‑1 (MQ=255)
                                                                                                            cTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGACGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCAc                                   >  1:1689115/1‑150 (MQ=255)
                                                                                                              tattatCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGACGTTCCTTAATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCAccc                                 >  2:1067897/1‑150 (MQ=255)
                                                                                                                 tatCGCAGCTAATCAATAAAATCCTGCCACATATCTACAATGGGCAGTTGATCATTATATTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAAc                              <  1:113449/150‑1 (MQ=255)
                                                                                                                  atCGCCGCTAATCAATAAAATCCTGCCCCATATCTCAATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAACACTCTGCCGTTCCGTCATCACCAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACg                             >  1:892862/1‑150 (MQ=255)
                                                                                                                   tCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGt                            <  1:883925/150‑1 (MQ=255)
                                                                                                                    cgccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCCCACGCCCGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGtt                           >  1:569434/1‑150 (MQ=255)
                                                                                                                         cTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTc                      <  1:1547169/150‑1 (MQ=255)
                                                                                                                          tAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTGGCGGCa                     >  1:1425438/1‑150 (MQ=255)
                                                                                                                            aTCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATa                   <  2:482360/150‑1 (MQ=255)
                                                                                                                            aTCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATa                   >  1:538185/1‑150 (MQ=255)
                                                                                                                             tCAATAAAATCCTGCCCCAAATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGGCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAg                  <  1:1497356/150‑1 (MQ=255)
                                                                                                                              cAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGc                 >  1:1398590/1‑150 (MQ=255)
                                                                                                                              cAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGc                 <  1:1345070/150‑1 (MQ=255)
                                                                                                                              cAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTAATTCTTTTAGTCTGGTAATTCACACCACAGCAAAAACTCTGCCGATCCTTCATAAAAAAACAGTACCGTGACATATACACCCAGCAGGGCACCCAACCTTGCATCATaac                 >  1:843317/1‑148 (MQ=255)
                                                                                                                              aaaTAAAATCCTGCACCATATCTACATGGTGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTGCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGc                 <  1:420089/149‑1 (MQ=255)
                                                                                                                               aaaaaaaTCCTGCCCAATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGcc                <  1:753335/147‑1 (MQ=255)
                                                                                                                               aaTAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTATTTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATACTCCCAGCAGGGCACCCAACGTTGCGTCAGAGcc                >  1:259622/1‑150 (MQ=255)
                                                                                                                               aaTAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGcc                >  1:1340301/1‑150 (MQ=255)
                                                                                                                               aaTAAAATCCTGCCCCATATATAAATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTACAAGCAGGGCACCCAACGTTGCGTCATAGcc                >  2:724840/1‑150 (MQ=255)
                                                                                                                                 tAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCctc              >  2:1085241/1‑150 (MQ=255)
                                                                                                                                    aaTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCttt           <  1:724832/150‑1 (MQ=255)
                                                                                                                                     caacTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGGTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTc          >  1:36763/4‑150 (MQ=255)
                                                                                                                                      tCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc         >  1:148953/1‑150 (MQ=255)
                                                                                                                                      tCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc         <  1:1526961/150‑1 (MQ=255)
                                                                                                                                       acTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTacc        >  2:506931/2‑150 (MQ=255)
                                                                                                                                        cTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcccc       >  1:1102192/1‑150 (MQ=255)
                                                                                                                                         tGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCcca      <  1:1100933/150‑1 (MQ=255)
                                                                                                                                            cccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacca   >  2:1408572/1‑150 (MQ=255)
                                                                                                                                             ccATATCTACATGGGGCAGTTGCTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCGCCAg  <  1:391896/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAG  >  NC_000913/4519166‑4519456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: