BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,523,472	A→C	7.0%	F208C (TTC→TGC)	yjhG ←	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,523,472	0	A	C	7.0%	322.2 / 11.4	110	F208C (TTC→TGC)	yjhG	putative dehydratase
Reads supporting (aligned to +/- strand): ref base A (51/51); new base C (2/6); total (53/57)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

Only 100 of 110 total aligned reads displayed.

TGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCAC  >  NC_000913/4523327‑4523620

tGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCg                                                                                                                                                  >  1:1503884/1‑150 (MQ=255)

 gAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGt                                                                                                                                                 >  2:165411/1‑150 (MQ=255)

    tgtgGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTgcg                                                                                                                                              >  2:305755/1‑150 (MQ=255)

      tgGGATTGCCCGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGcc                                                                                                                                            <  2:1065641/150‑1 (MQ=255)

           ttGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATgg                                                                                                                                       <  1:1151287/150‑1 (MQ=255)

           ttGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATgg                                                                                                                                       <  1:337721/150‑1 (MQ=255)

           ttGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATgg                                                                                                                                       <  1:859620/150‑1 (MQ=255)

           ttGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATgg                                                                                                                                       <  1:1657993/150‑1 (MQ=255)

            tGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATggc                                                                                                                                      <  1:502714/150‑2 (MQ=255)

            tGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATggc                                                                                                                                      <  2:562936/150‑2 (MQ=255)

             gCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGtt                                                                                                                                     <  2:880445/150‑1 (MQ=255)

             gCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGtt                                                                                                                                     >  2:681687/1‑150 (MQ=255)

               cAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTg                                                                                                                                   <  1:1150339/150‑1 (MQ=255)

               cAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTg                                                                                                                                   >  1:1688818/1‑150 (MQ=255)

                aGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGc                                                                                                                                  <  1:810509/150‑1 (MQ=255)

                 gTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATggcgaatt                                                                                                                                 <  1:1380639/150‑7 (MQ=255)

                 gTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCACCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCa                                                                                                                                 <  2:1438200/150‑1 (MQ=255)

                   cccAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTTGAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAcc                                                                                                                               >  2:849145/1‑150 (MQ=255)

                   cccAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAcc                                                                                                                               >  1:514523/1‑150 (MQ=255)

                   cccAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAcc                                                                                                                               >  1:787277/1‑150 (MQ=255)

                    ccAATCCTTCGGCCACCACCGGCTATGTCCCGGCAGTGCCCAAAAATTCACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCt                                                                                                                              <  1:156620/150‑1 (MQ=255)

                       aTCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGc                                                                                                                           <  2:1238346/150‑1 (MQ=255)

                         ccTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACCGGCTTTACAGCCCGCCCGGCGTGCGTCCTGTAGAGATAATTCGCCATAGGCGAAGCGTGCGCCAATGGTGTGGACATTGACg                                                                                                                         >  1:1157338/1‑150 (MQ=255)

                         ccTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCCCGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCg                                                                                                                         >  1:1616014/1‑150 (MQ=255)

                         ccTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCGCCTTGCCg                                                                                                                         >  1:68430/1‑150 (MQ=255)

                          cTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGt                                                                                                                        <  2:1199478/150‑1 (MQ=255)

                            tCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTg                                                                                                                      <  2:1294496/150‑1 (MQ=255)

                            tCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTg                                                                                                                      <  2:1613210/150‑1 (MQ=255)

                            tCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTg                                                                                                                      <  2:1494645/150‑1 (MQ=255)

                               gCCACCACCTGACATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCAGAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtct                                                                                                                   <  2:698777/150‑1 (MQ=255)

                                 caccacCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtcttc                                                                                                                 <  2:1358656/150‑1 (MQ=255)

                                   ccaccTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTtctc                                                                                                               <  2:1625829/150‑1 (MQ=255)

                                    caccTGAGTTGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGATTCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTcc                                                                                                              <  2:45744/150‑1 (MQ=255)

                                    caccTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTcc                                                                                                              >  1:1197496/1‑150 (MQ=255)

                                      ccTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGAGTGCGCCAATGGTTTGCACCTTGCCGTAGTCTTCTCCaa                                                                                                            >  2:1431414/1‑149 (MQ=255)

                                        tGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATcc                                                                                                          >  2:21285/1‑150 (MQ=255)

                                           gaTGTCCCGGCAGTGCCCAAAAATTGCCAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCTTCCttt                                                                                                       >  2:205620/1‑150 (MQ=255)

                                           gaTGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCttt                                                                                                       >  2:523966/1‑150 (MQ=255)

                                            aTGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTg                                                                                                      >  1:800588/1‑150 (MQ=255)

                                               gcccGGCAGTGCCCAAAAATTGACAGCAGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCgg                                                                                                   <  1:1436291/149‑1 (MQ=255)

                                                cccGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCGGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCGTGCCGTTGTCTTCTCCATCCTTTGCggg                                                                                                  >  1:163244/1‑150 (MQ=255)

                                                     cAGTGCCTAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCg                                                                                             >  1:273927/1‑150 (MQ=255)

                                                     cAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCg                                                                                             >  2:11353/1‑150 (MQ=255)

                                                     cAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCg                                                                                             >  2:1613820/1‑150 (MQ=255)

                                                         gCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGGAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGGGTGCCCCTTGCCGTTGTCGTCGCCATCCTTTGCGGGCCGCGTcgc                                                                                         >  1:120940/1‑150 (MQ=255)

                                                          cccAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTcgcg                                                                                        <  1:730314/150‑1 (MQ=255)

                                                           ccAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCgcgc                                                                                       <  2:1253263/150‑1 (MQ=255)

                                                            cAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTGGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGcgcc                                                                                      >  2:1488833/1‑150 (MQ=255)

                                                            cAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGcgcc                                                                                      >  2:1554968/1‑150 (MQ=255)

                                                                 aTTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCgg                                                                                 <  2:877201/150‑1 (MQ=255)

                                                                  ttGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCggg                                                                                <  1:579075/150‑1 (MQ=255)

                                                                      cAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGAcc                                                                            <  1:1093582/150‑1 (MQ=255)

                                                                            ccgccgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACg                                                                      >  1:763442/1‑150 (MQ=255)

                                                                              gccgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGt                                                                    >  1:698554/1‑150 (MQ=255)

                                                                                cgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTg                                                                  <  1:601090/150‑1 (MQ=255)

                                                                                       ggCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGAtgtt                                                           >  1:1001712/1‑150 (MQ=255)

                                                                                        gCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGAtgttg                                                          >  2:129543/1‑150 (MQ=255)

                                                                                        gCACAGGCTATACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGAtgttg                                                          <  1:1196651/150‑1 (MQ=255)

                                                                                                tctACAGGACGCACGCCGTGCGGGCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTgcgc                                                  >  1:506990/3‑150 (MQ=255)

                                                                                                 ctACAGGACGCACGCCGTGCGGGCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGcgcg                                                 >  2:566658/2‑150 (MQ=255)

                                                                                                  tACAGCCCGCTCGGCGTGCGTCCTGTAGAGATACTTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcgg                                                <  2:1375799/150‑1 (MQ=255)

                                                                                                  tACAGCCCGCACGGCGTGGGTCCTGTTGAGATAATTCGCCCTTGGCGAAGCGTGCGCCAAAGGTTTGCTACTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcgg                                                <  2:867829/150‑1 (MQ=255)

                                                                                                   acaggacGCACGCCGTGCGGGCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcggc                                               >  2:1321166/7‑150 (MQ=255)

                                                                                                   aCAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcggc                                               >  2:934099/1‑150 (MQ=255)

                                                                                                    caggacGCACGCCGTGCGGGCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcggcg                                              >  2:357557/6‑150 (MQ=255)

                                                                                                    caggacGCACGCCGTGCGGGCTGTAAAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcggcg                                              >  2:704136/6‑150 (MQ=255)

                                                                                                      gCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAg                                            <  1:1370699/150‑1 (MQ=255)

                                                                                                      gCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAg                                            <  2:468511/150‑1 (MQ=255)

                                                                                                       cccGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGt                                           >  1:596752/1‑150 (MQ=255)

                                                                                                        ccGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTg                                          >  2:596767/1‑150 (MQ=255)

                                                                                                        ccGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTg                                          <  1:508001/150‑1 (MQ=255)

                                                                                                          gCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGcc                                        <  1:11353/150‑1 (MQ=255)

                                                                                                          gCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGcc                                        <  2:1503884/150‑1 (MQ=255)

                                                                                                           cACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCca                                       <  2:273927/150‑1 (MQ=255)

                                                                                                               gcgtgcgtCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGACAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGGTGCGATGTTGTGCTGCGCGGCGAGTGCcatcat                                   >  1:52149/1‑150 (MQ=255)

                                                                                                                 gtgcgtCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCAtgg                                 <  2:1421950/150‑1 (MQ=255)

                                                                                                                  tgcgtCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCAtggt                                >  1:365151/1‑150 (MQ=255)

                                                                                                                   gcgtCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGTGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCAtggtg                               <  2:700753/150‑1 (MQ=255)

                                                                                                                    cgtCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCAtggtgg                              <  2:1376286/150‑1 (MQ=255)

                                                                                                                      tCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGcc                            <  2:1686579/150‑1 (MQ=255)

                                                                                                                      gccTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGcc                            <  1:463449/149‑1 (MQ=255)

                                                                                                                       ccTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCg                           <  1:1321824/150‑1 (MQ=255)

                                                                                                                        cTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCgg                          >  1:1077431/1‑150 (MQ=255)

                                                                                                                         tGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCCATGGTTTGCACCTTGCCGTTGTCTTCTCCCTCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCCGCACGGTTGCGTTGTTGTGCTGCGCGGCGAGGGCCATCATGGTGGCCGGa                         >  1:249479/1‑150 (MQ=255)

                                                                                                                            agagATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCTTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCAACATGGTGGCCGGAAGc                      >  2:132379/1‑150 (MQ=255)

                                                                                                                            agagATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGc                      <  2:1634936/150‑1 (MQ=255)

                                                                                                                            agagATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGc                      >  1:897798/1‑150 (MQ=255)

                                                                                                                             gagaTAATTCGCCATTGGCGAAGCGGGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGGGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGcc                     <  2:314755/150‑1 (MQ=255)

                                                                                                                               cacgcttCGCCAATGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGAGCCATCATGGTGGCCGGAAGcccc                   >  2:823934/6‑150 (MQ=255)

                                                                                                                                aTAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCt                  <  1:1375614/150‑1 (MQ=255)

                                                                                                                                    ttCGCCATTGGCGCAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATc              >  1:1695031/1‑150 (MQ=255)

                                                                                                                                    ttCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTGGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGCGTGCCCTCATGGTGGCCGGCCGCCCCTTATc              >  2:1039407/1‑150 (MQ=255)

                                                                                                                                     acgcccatGGCGAAGCGTGCGCCACTGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCg             <  2:1616014/143‑1 (MQ=255)

                                                                                                                                        ccATTGGCGCACGCTTCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCCTCATGGTGGCCGGAAGCCCCTTATCGCac          >  1:1552329/1‑149 (MQ=255)

                                                                                                                                            tGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCg      <  1:783445/150‑1 (MQ=255)

                                                                                                                                             ggCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGc     <  1:388085/150‑1 (MQ=255)

                                                                                                                                             ggCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGc     >  2:1310059/1‑150 (MQ=255)

                                                                                                                                             ggCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGc     >  1:166351/1‑150 (MQ=255)

                                                                                                                                              gCGAAGCGCGCGCCACTGGTTTGCACCTTGCCGTAGTCTTCTCCATCCTTTGCGGGCCGCGGCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGcc    <  1:183915/150‑1 (MQ=255)

                                                                                                                                                gAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCcac  <  1:512969/150‑1 (MQ=255)

TGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCCAC  >  NC_000913/4523327‑4523620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`