Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 568,696 T→A 7.3% intergenic (+49/‑206) emrE → / → ybcK DLP12 prophage; multidrug resistance protein/DLP12 prophage; putative recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913568,6960TA 7.3% 386.4 / 12.1 133intergenic (+49/‑206)emrE/ybcKDLP12 prophage; multidrug resistance protein/DLP12 prophage; putative recombinase
Reads supporting (aligned to +/- strand):  ref base T (66/57);  new base A (3/7);  total (69/64)
Fisher's exact test for biased strand distribution p-value = 1.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

TTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAA  >  NC_000913/568547‑568844
                                                                                                                                                     |                                                                                                                                                    
ttttCGTCCTACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATATGTGCCTGTGTGTTGATTATTACTTTATTGTCACGAAGCACACCACATTAAACTAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttatatttatat                                                                                                                                                      <  1:97557/150‑1 (MQ=255)
ttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttatatttatat                                                                                                                                                      <  2:1358008/150‑1 (MQ=255)
   tCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAg                                                                                                                                                   <  2:626068/150‑1 (MQ=255)
   tCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAagag                                                                                                                                                   <  2:529595/150‑1 (MQ=255)
    cGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGc                                                                                                                                                  >  1:1728000/1‑150 (MQ=255)
    cGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGc                                                                                                                                                  <  1:1569012/150‑1 (MQ=255)
     ggCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGcc                                                                                                                                                 <  1:424587/150‑1 (MQ=255)
      gCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCt                                                                                                                                                <  1:209884/150‑1 (MQ=255)
      gCCAACGGCTGGACCTGCAAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCt                                                                                                                                                >  1:986186/1‑150 (MQ=255)
       ccAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCTc                                                                                                                                               <  2:456163/150‑1 (MQ=255)
       ccAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCTc                                                                                                                                               <  1:1220522/150‑1 (MQ=255)
        cAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCg                                                                                                                                              >  2:133580/1‑150 (MQ=255)
          aCGGCTGGTCCTGCCAGCCATAATAGTGATTATTTTGATTTGTGCCGGTTTTTTGATTTTTAATTTATTGTCACGTAGCAGACCACATTAAAATAATTTGTTTCTAAACGTCTAAAATATGGAGGCTCATATATATATAAGAGCCTccat                                                                                                                                            <  1:1001201/150‑4 (MQ=255)
          aCGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTTTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCTccat                                                                                                                                            <  1:848256/150‑4 (MQ=255)
            ggCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGtttt                                                                                                                                          <  2:1379814/150‑1 (MQ=255)
             gCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGttttt                                                                                                                                         >  1:301020/1‑149 (MQ=255)
             gCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTa                                                                                                                                         <  2:737172/150‑1 (MQ=255)
             gCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTa                                                                                                                                         <  2:1008383/150‑1 (MQ=255)
              cTGGACCAGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTACAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGGTTTAt                                                                                                                                        <  1:286687/150‑1 (MQ=255)
                ggACCTGCCAGCCATTATTTGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCTCGAAGCACACCACATTAATATAATTTGTTTCAAAACGCCTAAAATATGGAGGCTGTTATATTTATATGAGCCTCGTTTTATGc                                                                                                                                      <  2:245415/150‑1 (MQ=255)
                    cTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCtttt                                                                                                                                  <  2:1409206/150‑1 (MQ=255)
                      gccagccaTTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTg                                                                                                                                <  2:1096253/150‑1 (MQ=255)
                        cagccaTTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGtt                                                                                                                              >  1:936960/1‑150 (MQ=255)
                               tataGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCt                                                                                                                       >  2:527203/1‑150 (MQ=255)
                               tataGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCt                                                                                                                       >  2:135471/1‑150 (MQ=255)
                                ataGGCATGATGTTGATTTGTGCCGGTGTGTTGATTAATAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCtt                                                                                                                      <  1:696625/150‑1 (MQ=255)
                                   ggCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAt                                                                                                                   >  1:478692/1‑150 (MQ=255)
                                     cATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAttt                                                                                                                 >  2:678422/1‑150 (MQ=255)
                                           gTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGATGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTAtt                                                                                                           >  1:877555/1‑149 (MQ=255)
                                             tGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTa                                                                                                         >  2:857103/1‑150 (MQ=255)
                                              gATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTAt                                                                                                        >  1:1662572/1‑150 (MQ=255)
                                              gATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTAt                                                                                                        >  1:772169/1‑150 (MQ=255)
                                              gATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTAt                                                                                                        >  2:550190/1‑150 (MQ=255)
                                               aTTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTAtt                                                                                                       >  2:1706614/1‑150 (MQ=255)
                                                 ttGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCt                                                                                                     <  1:831926/150‑1 (MQ=255)
                                                     gCCGGTGTTTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtg                                                                                                 <  1:1163043/150‑1 (MQ=255)
                                                     gCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtg                                                                                                 <  2:512086/150‑1 (MQ=255)
                                                       cGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtgtg                                                                                               <  2:586696/150‑1 (MQ=255)
                                                        ggTTTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGc                                                                                              <  1:543703/150‑1 (MQ=255)
                                                        ggTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATg                                                                                                           <  2:1353327/137‑1 (MQ=255)
                                                        ggTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATg                                                                                                           >  1:1353327/1‑137 (MQ=255)
                                                        ggTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGc                                                                                              <  1:1600583/150‑1 (MQ=255)
                                                           gtgtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCttt                                                                                           <  2:1181643/150‑1 (MQ=255)
                                                             gtTGATTATTAATTTATTGTCACGACGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAATTATGGAGGCTCTTATATTTATATGAGCGTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCa                                                                                         >  1:1088853/1‑150 (MQ=255)
                                                                 tttattaATTTATTGTCACGAAGCACACCACATCAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATGTGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCGTTCAAGAt                                                                                     <  1:1630674/149‑1 (MQ=255)
                                                                  ttattaATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGAtt                                                                                    <  2:642795/150‑1 (MQ=255)
                                                                     ttaATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATg                                                                                 >  1:331769/1‑150 (MQ=255)
                                                                     ttaATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATg                                                                                 <  1:365441/150‑1 (MQ=255)
                                                                      taATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTCTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATgg                                                                                >  2:1254371/1‑150 (MQ=255)
                                                                        aTTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCg                                                                              <  1:1717254/150‑1 (MQ=255)
                                                                          ttATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTa                                                                            <  1:1612577/150‑1 (MQ=255)
                                                                           tATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTaa                                                                           >  1:1692497/1‑150 (MQ=255)
                                                                            aTTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAg                                                                          >  1:1137352/1‑150 (MQ=255)
                                                                               gTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGaaa                                                                       >  1:1356837/1‑150 (MQ=255)
                                                                                   cGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCAGCGTTTTATGATTTTTTTTATGGGTTTAATTTTTTATTTTTTATTTTTTGATTTAAATATATTGGCGTAAAAAAATTg                                                                   >  2:126771/1‑150 (MQ=255)
                                                                                    gAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGc                                                                  <  1:1060231/150‑1 (MQ=255)
                                                                                     aaGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCa                                                                 <  2:1219601/150‑1 (MQ=255)
                                                                                      aGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCaa                                                                <  1:617658/150‑1 (MQ=255)
                                                                                           accacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGa                                                           >  1:1259553/1‑150 (MQ=255)
                                                                                            ccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGat                                                          >  1:1026120/1‑150 (MQ=255)
                                                                                            ccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTATGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGat                                                          >  2:508770/1‑150 (MQ=255)
                                                                                               caTTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGattat                                                       >  2:195337/1‑150 (MQ=255)
                                                                                               aaTTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGattat                                                       <  2:1536224/149‑1 (MQ=255)
                                                                                                aTTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGattatt                                                      >  2:90072/1‑150 (MQ=255)
                                                                                                aTTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGattatt                                                      <  2:417248/150‑1 (MQ=255)
                                                                                                   aaaaTAATTTGTTTCTAAAAGACTAAAATATGGAGGATCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTGTTTTGTGCTATAAATATAATGCCGTAAGAAAATTGAAATAATATTAttgtt                                                   >  2:734260/1‑150 (MQ=255)
                                                                                                     aataatTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATtgttgt                                                 <  2:1110269/150‑1 (MQ=255)
                                                                                                          tttGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATAtt                                            <  2:1505472/150‑1 (MQ=255)
                                                                                                          tttGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATAtt                                            >  2:604332/1‑150 (MQ=255)
                                                                                                           ttGTTTCTAAACGACAAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTc                                           <  2:377294/150‑1 (MQ=255)
                                                                                                             gTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCaa                                         >  1:624256/1‑150 (MQ=255)
                                                                                                               ttCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGa                                       >  2:183966/1‑150 (MQ=255)
                                                                                                                tCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGAt                                      >  2:1167632/1‑150 (MQ=255)
                                                                                                                 cTAAACGACTAAAATATGGAGGCTGTTATTAATATATGAGCCTCGTTTTATGCTTTTTGTGAATGACTTTAATTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGAGTAAGAAAATTGCAATAGCATTATTTTTTTATAATCAAGATa                                     >  2:1522763/1‑150 (MQ=255)
                                                                                                                   aaaCGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAAt                                   >  2:1547499/1‑150 (MQ=255)
                                                                                                                    aaCGACTAAAATATGGGGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAAtg                                  <  2:1005266/150‑1 (MQ=255)
                                                                                                                    aaCGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAAtg                                  >  2:567207/1‑150 (MQ=255)
                                                                                                                      cGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAAtgtg                                >  2:573666/1‑150 (MQ=255)
                                                                                                                         cTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGTCGTAAGAAAATTGCAATACGATAATTGTTGTATATTCAAGATAATGTGAcc                             >  2:182086/1‑150 (MQ=255)
                                                                                                                          tAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCt                            <  2:375170/150‑1 (MQ=255)
                                                                                                                            ataaaacgAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTTTTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTa                          >  2:1068114/8‑150 (MQ=255)
                                                                                                                               aaacgAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAAtt                       >  2:552253/5‑150 (MQ=255)
                                                                                                                                atGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTg                      <  1:148079/150‑1 (MQ=255)
                                                                                                                                atGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTg                      <  2:1662572/150‑1 (MQ=255)
                                                                                                                                 tGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTTTGTATTCTTTTGTGCTTTCAAGATTATGGAGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGt                     >  2:183779/1‑150 (MQ=255)
                                                                                                                                   gAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGTCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCt                   >  2:942626/1‑150 (MQ=255)
                                                                                                                                   gAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCt                   >  2:1412710/1‑150 (MQ=255)
                                                                                                                                   gAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCt                   <  1:1521967/150‑1 (MQ=255)
                                                                                                                                    aGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACTATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCtt                  >  1:1223515/1‑150 (MQ=255)
                                                                                                                                      gCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTTTGCTTTCAAGATTATGGCGTAAGATATTTGCTATACGATTATTGTTGTATATTCATGATAATGTGACCTTAATTGTCtttt                <  1:1007978/150‑1 (MQ=255)
                                                                                                                                       ctctTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCttttt               <  2:478692/150‑1 (MQ=255)
                                                                                                                                       ctctTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCttttt               >  2:296677/1‑150 (MQ=255)
                                                                                                                                       ctcaTATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCttttt               >  1:1621219/1‑150 (MQ=255)
                                                                                                                                         ctTATATTTATATGAGCCTCGTTTTATTCTTTTTGTTAATGTATTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTtaa             <  1:873034/150‑1 (MQ=255)
                                                                                                                                              atttatatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTtaaataaa        <  1:476618/150‑1 (MQ=255)
                                                                                                                                               tttatatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTaaataaaa       <  1:519641/150‑1 (MQ=255)
                                                                                                                                                ttatatGTTGCTCGTTTTATGCTTTTTGTTAATGTATTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTaaataaaaa      <  1:126771/150‑1 (MQ=255)
                                                                                                                                                ttatatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTaaataaaaa      <  2:1043130/150‑1 (MQ=255)
                                                                                                                                                  atatGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTaaataaaaaat    >  2:847357/1‑150 (MQ=255)
                                                                                                                                                    atGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAATTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTaaataaaaaataa  <  2:302777/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTAAATAAAAAATAA  >  NC_000913/568547‑568844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: