Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,549,873 A→C 6.5% intergenic (+163/+80) fimH → / ← gntP minor component of type 1 fimbriae/fructuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,549,8730AC 6.5% 320.2 / 11.4 129intergenic (+163/+80)fimH/gntPminor component of type 1 fimbriae/fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base A (54/66);  new base C (2/7);  total (56/73)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01

Only 100 of 131 total aligned reads displayed.

CATTGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGAC  >  NC_000913/4549724‑4550020
                                                                                                                                                     |                                                                                                                                                   
cATTGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGACCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAAAAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTCACCCTCTCCCCGGa                                                                                                                                                     >  1:102394/1‑150 (MQ=255)
 aTTGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAg                                                                                                                                                    >  1:659031/1‑150 (MQ=255)
   tGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAggg                                                                                                                                                  <  1:1016741/150‑1 (MQ=255)
   tGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAggg                                                                                                                                                  >  2:941366/1‑150 (MQ=255)
    gCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAgggg                                                                                                                                                 >  1:473380/1‑150 (MQ=255)
    gCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCACTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAACGCGCACGTTCCTGCTCTGTTCCCCTCCCCCTACCCCTCTCCCCCGAggcc                                                                                                                                                 >  1:257072/1‑148 (MQ=38)
     cTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGc                                                                                                                                                <  1:601707/150‑1 (MQ=255)
        aTGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAg                                                                                                                                             <  1:1438152/150‑1 (MQ=255)
         tGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAgg                                                                                                                                            <  2:640500/150‑1 (MQ=255)
         tGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCGCCCCTCCGGGGAGAgg                                                                                                                                            <  2:1268246/150‑1 (MQ=39)
          gCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTTGCCGTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAggg                                                                                                                                           <  2:342884/150‑1 (MQ=255)
          gCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAggg                                                                                                                                           >  2:676966/1‑150 (MQ=255)
          gCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCAGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAggg                                                                                                                                           >  1:1376010/1‑150 (MQ=255)
           cTGGTACGCAATATTACCTGAAGCTAAAAACCTGCCCGTTAGCCCTTTGTAGGCCAGATAAGACGCGGCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCGCCCCTCCGGGGAGAgggt                                                                                                                                          <  1:1273483/150‑2 (MQ=255)
           cTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAgggg                                                                                                                                          >  2:1349128/1‑150 (MQ=255)
            tGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGa                                                                                                                                         <  2:663727/150‑1 (MQ=255)
             ggTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGAc                                                                                                                                        <  1:921449/150‑1 (MQ=255)
             ggTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGAc                                                                                                                                        <  2:202977/150‑1 (MQ=255)
             ggTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGAc                                                                                                                                        <  2:845592/150‑1 (MQ=255)
               caCGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCGCCCCTCCGGGGAGAgggttagg                                                                                                                                      <  1:1626565/149‑6 (MQ=37)
                aCGCAATATTACCTGACGCTAAAAACCTGCACGTTATCCCTTTGTAGGCCAGATAAGACGCATCCGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCCCCCTACCCCTCGCCCCTCCGGGGAGAgggttaggg                                                                                                                                     <  2:68649/150‑7 (MQ=255)
                 cGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTc                                                                                                                                    >  1:271135/1‑150 (MQ=255)
                      tatTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGc                                                                                                                               >  2:1700399/1‑150 (MQ=255)
                          aCCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACAt                                                                                                                           >  1:519096/1‑150 (MQ=255)
                           ccTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACAtt                                                                                                                          >  2:1594706/1‑150 (MQ=255)
                            cTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACAttt                                                                                                                         >  1:1353791/1‑150 (MQ=255)
                             tGAAGCTCAAAACCTGCACGGTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACAtttt                                                                                                                        <  2:961219/150‑1 (MQ=255)
                              gAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACAttttt                                                                                                                       >  2:1028093/1‑150 (MQ=255)
                                aGCTAAAAACCTGCATGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCAACCTACCCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAg                                                                                                                     <  1:59383/150‑1 (MQ=255)
                                  cTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCCCATTTTTAGAc                                                                                                                   >  2:116127/1‑150 (MQ=255)
                                  cTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGAc                                                                                                                   >  1:372655/1‑150 (MQ=255)
                                  cTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGAc                                                                                                                   >  1:378863/1‑150 (MQ=255)
                                        aCCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGGTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtc                                                                                                             <  1:685041/150‑1 (MQ=255)
                                          cTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcatc                                                                                                          <  2:793088/150‑1 (MQ=255)
                                           tGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcatc                                                                                                          <  2:108922/150‑1 (MQ=255)
                                           tGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcatc                                                                                                          <  2:128904/150‑1 (MQ=255)
                                           tGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcatc                                                                                                          >  2:1364900/1‑150 (MQ=255)
                                            gCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGtcatca                                                                                                         <  2:43347/150‑1 (MQ=255)
                                             cACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAg                                                                                                        <  1:1150536/150‑1 (MQ=255)
                                               cGTTAGCCCTTTGTAGGCCTGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTc                                                                                                      <  1:480524/150‑1 (MQ=255)
                                                 ttAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTg                                                                                                    <  2:1362031/150‑1 (MQ=255)
                                                     cccTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGcc                                                                                                <  1:651907/150‑1 (MQ=255)
                                                        tttGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGc                                                                                             <  1:1058317/150‑1 (MQ=255)
                                                             aGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTg                                                                                        >  2:650266/1‑150 (MQ=255)
                                                               gCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGc                                                                                      >  1:1421792/1‑150 (MQ=255)
                                                                 cAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAg                                                                                    <  2:310512/150‑1 (MQ=255)
                                                                   gATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCTATTGGCAGGc                                                                                  <  1:1280237/150‑1 (MQ=255)
                                                                     tAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctc                                                                                <  1:1700399/150‑1 (MQ=255)
                                                                      aaGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGctct                                                                               >  2:1449442/1‑150 (MQ=255)
                                                                       aGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTATGAGCCTGCCATTGGCAGGCTCTg                                                                              >  1:1118391/1‑150 (MQ=255)
                                                                        gACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGCGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTgg                                                                             <  1:783687/150‑1 (MQ=255)
                                                                        gACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTgg                                                                             <  1:1590465/150‑1 (MQ=255)
                                                                         aCGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCAATGGCAGGctcagac                                                                            <  1:1086192/150‑5 (MQ=255)
                                                                          cgcgTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCCGAGCCTGCCATTGGCAGGCTCTGgtg                                                                           >  2:781382/1‑150 (MQ=255)
                                                                            cgTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTc                                                                         >  1:831567/1‑150 (MQ=255)
                                                                             gTCAGCGTCGCATCTGGCATAATCAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTcc                                                                        <  1:62624/150‑1 (MQ=255)
                                                                             gTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGGCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTcc                                                                        <  2:568938/150‑1 (MQ=255)
                                                                             gTCACCGTAGCATCTGGCATAAACAAAGCGCACTATGCTGGTCTGTTCCCCTCACCCTAACCCACTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTcc                                                                        <  1:328654/150‑1 (MQ=255)
                                                                                   gTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAc                                                                  <  2:473380/150‑1 (MQ=255)
                                                                                       cATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTa                                                              >  1:1046529/1‑150 (MQ=255)
                                                                                           tGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAc                                                                  <  2:42022/142‑1 (MQ=255)
                                                                                           tGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTAc                                                                  >  1:42022/1‑142 (MQ=255)
                                                                                             gCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                                                        >  1:1540938/1‑150 (MQ=255)
                                                                                             gCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGc                                                        >  2:709072/1‑150 (MQ=255)
                                                                                              cATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                       >  2:663614/1‑150 (MQ=255)
                                                                                              cATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                       >  1:371477/1‑150 (MQ=255)
                                                                                              cATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                       >  1:1729177/1‑150 (MQ=255)
                                                                                              cATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCt                                                       <  1:611103/150‑1 (MQ=255)
                                                                                                 aaaCAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCtaat                                                    <  2:1247694/150‑1 (MQ=255)
                                                                                                 aaaCAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCtaat                                                    <  1:284855/150‑1 (MQ=255)
                                                                                                   aCAAAGCGCACTTTGCTGGTCTGTTCCCCTCACACTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCtaataa                                                  >  1:149926/1‑150 (MQ=255)
                                                                                                    cAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTaataat                                                 >  1:124974/1‑150 (MQ=255)
                                                                                                     aaaGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATc                                                <  2:372287/150‑1 (MQ=255)
                                                                                                       aGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAg                                              <  2:462629/150‑1 (MQ=255)
                                                                                                        gcgcACATTGCTGGTCTGTTCCCCTCAACCTAACCCTCTCCCCGTAGGGGCGAGGGGACTGTCCGGGCACATTTTTATACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGc                                             <  1:949482/150‑1 (MQ=255)
                                                                                                                  cTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCa                                   >  1:457045/1‑150 (MQ=255)
                                                                                                                  cTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCa                                   <  2:372655/150‑1 (MQ=255)
                                                                                                                  cTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCa                                   >  1:1098811/1‑150 (MQ=255)
                                                                                                                      tCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCCGccc                               >  2:1281949/1‑150 (MQ=255)
                                                                                                                       cTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCa                              <  1:709072/150‑1 (MQ=255)
                                                                                                                            ccccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCTGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCAc                         <  2:1376010/150‑1 (MQ=255)
                                                                                                                              ccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACgg                       >  1:659957/1‑150 (MQ=255)
                                                                                                                              ccTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACgg                       <  1:66100/150‑1 (MQ=255)
                                                                                                                                tCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAg                     <  2:831567/150‑1 (MQ=255)
                                                                                                                                tCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAg                     >  2:632573/1‑150 (MQ=255)
                                                                                                                                   cccTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTg                  >  1:568273/1‑150 (MQ=255)
                                                                                                                                     cTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAc                <  1:1012778/150‑1 (MQ=255)
                                                                                                                                     cTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGAc                <  2:519096/150‑1 (MQ=255)
                                                                                                                                       aaCCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCa              <  1:941366/150‑1 (MQ=255)
                                                                                                                                        ccccTCGCCCCTCCGGGGAGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCACCCACGGAGTTGACCAg             >  1:933771/2‑150 (MQ=255)
                                                                                                                                        aCCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAg             >  1:371704/1‑150 (MQ=255)
                                                                                                                                         cccTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTa                                                                   >  1:356296/1‑95 (MQ=255)
                                                                                                                                         cccTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGc            <  2:39428/150‑1 (MQ=255)
                                                                                                                                            tctcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTcc         <  1:676966/150‑1 (MQ=255)
                                                                                                                                             ctcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCCACCACGGAGTTGCCCAGCTCca        >  2:25887/1‑150 (MQ=255)
                                                                                                                                             ctcCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCca        >  2:359983/1‑150 (MQ=255)
                                                                                                                                                 ccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCcagcag    <  1:1000476/150‑1 (MQ=255)
                                                                                                                                                 ccGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTAATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCcagcag    <  2:149926/150‑1 (MQ=255)
                                                                                                                                                   ggAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGAc  >  2:884763/1‑150 (MQ=255)
                                                                                                                                                   ggAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTATGTCATCAGTCTGAGCCTGCAATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCCTCACAAAAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCacac  >  2:334420/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CATTGCTAATGCTGGTACGCAATATTACCTGAAGCTAAAAACCTGCACGTTAGCCCTTTGTAGGCCAGATAAGACGCGTCAGCGTCGCATCTGGCATAAACAAAGCGCACTTTGCTGGTCTGTTCCCCTCACCCTAACCCTCTCCCCGGAGGGGCGAGGGGACTGTCCGGGCACATTTTTAGACTTTGTCATCAGTCTGAGCCTGCCATTGGCAGGCTCTGGTGTCCTTTTACGCTACCATGCTAATAATCAGCACAATAATCAGCCCAACCACGGAGTTGACCAGCTCCAGCAGAC  >  NC_000913/4549724‑4550020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: