Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,551,422 T→A 8.5% intergenic (‑126/‑214) gntP ← / → uxuA fructuronate transporter/mannonate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,551,4220TA 8.5% 402.5 / 18.5 145intergenic (‑126/‑214)gntP/uxuAfructuronate transporter/mannonate hydrolase
Reads supporting (aligned to +/- strand):  ref base T (57/75);  new base A (3/10);  total (60/85)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

GAGAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATAC  >  NC_000913/4551276‑4551571
                                                                                                                                                  |                                                                                                                                                     
gagaATGTTAATCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTg                                                                                                                                                    <  2:86715/150‑1 (MQ=255)
  gaATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgca                                                                                                                                                  <  1:117688/150‑1 (MQ=255)
   aaTGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgcag                                                                                                                                                 <  2:206435/150‑1 (MQ=255)
    aTGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTgcagc                                                                                                                                                <  1:1409536/150‑1 (MQ=255)
    aTGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTgcagc                                                                                                                                                <  2:1147154/150‑1 (MQ=255)
      gTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGcaa                                                                                                                                              >  2:396150/1‑150 (MQ=255)
        tAATCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGTGCGATATGTTATTTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaag                                                                                                                                            <  1:104189/150‑3 (MQ=255)
        tAATCACATGCATAAAGTTTTACCTTACCTGTTTGAACCGTTGTTATTTTGGGCGATATTTTATGTATATTGGTCAACCATTGTTGCGATGAATGTAACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaag                                                                                                                                            <  2:1105546/150‑3 (MQ=255)
        tAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaag                                                                                                                                            <  2:669480/150‑3 (MQ=255)
        tAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAAATTGCAGcaaag                                                                                                                                            <  2:378200/150‑3 (MQ=255)
            cacaTGCATTACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCa                                                                                                                                        <  1:270373/150‑1 (MQ=255)
            cacaTGCATAACGTTTTACCTTACCTGGTTGACCCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCa                                                                                                                                        >  2:631397/1‑150 (MQ=255)
             acaTGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCAt                                                                                                                                       <  2:1684463/150‑1 (MQ=255)
                 gCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATgaga                                                                                                                                   <  2:1424418/150‑1 (MQ=255)
                 gCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATgaga                                                                                                                                   <  1:1515560/150‑1 (MQ=255)
                  cATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGagag                                                                                                                                  <  2:196074/150‑1 (MQ=255)
                    tAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTg                                                                                                                                >  2:1156767/1‑150 (MQ=255)
                     aaCGTTTTACCTTACCTGTTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTTCAATTTGCAGCAACAACCATGAGAGTGa                                                                                                                               <  2:458304/150‑1 (MQ=255)
                        gTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAAt                                                                                                                            <  1:815190/150‑1 (MQ=255)
                           ttaccttaccTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCt                                                                                                                         <  1:602750/150‑1 (MQ=255)
                             accttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtg                                                                                                                       <  1:449571/150‑1 (MQ=255)
                             accttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtg                                                                                                                       <  1:1682075/150‑1 (MQ=255)
                             accttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtg                                                                                                                       <  1:1277261/150‑1 (MQ=255)
                               cttacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGAGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTtgtg                                                                                                                     <  1:57759/150‑1 (MQ=255)
                                 tacctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGAt                                                                                                                   <  2:52771/150‑1 (MQ=255)
                                  acctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGAtg                                                                                                                  <  2:523686/150‑1 (MQ=255)
                                   cctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGAtgt                                                                                                                 <  2:1627048/150‑1 (MQ=255)
                                    ctGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGAtgtg                                                                                                                <  2:586018/150‑1 (MQ=255)
                                      ggTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGt                                                                                                              >  1:1027631/1‑150 (MQ=255)
                                       gTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTTTGATGTGGtt                                                                                                             >  1:1286477/1‑150 (MQ=255)
                                       gTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGtt                                                                                                             >  1:893943/1‑150 (MQ=255)
                                           aaCCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAAcc                                                                                                         >  1:579448/1‑150 (MQ=255)
                                               gttgttATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAAtt                                                                                                     >  1:1442025/1‑150 (MQ=255)
                                                         tGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCg                                                                                           <  2:366149/150‑1 (MQ=255)
                                                           ggCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGcaacaa                                                                                                                                           <  1:1108106/100‑1 (MQ=255)
                                                           ggCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGcaacaa                                                                                                                                           >  2:1108106/1‑100 (MQ=255)
                                                           ggCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCggg                                                                                         <  2:1309365/150‑1 (MQ=255)
                                                           ggCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCggg                                                                                         <  2:607243/150‑1 (MQ=255)
                                                           ggCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCggg                                                                                         <  1:854982/150‑1 (MQ=255)
                                                           ggCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATACTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGAAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCggg                                                                                         <  1:237058/150‑1 (MQ=255)
                                                            gCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGt                                                                                        <  1:1149074/150‑1 (MQ=255)
                                                              gATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTg                                                                                      >  1:1442869/1‑150 (MQ=255)
                                                              gATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGGGGTTAACCAATTTCAGAATTCGGGTTg                                                                                      <  1:298026/150‑1 (MQ=255)
                                                                 atgttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCAGATCCTCTGATCAATATCCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACa                                                                                   <  2:294535/150‑1 (MQ=255)
                                                                  tgttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCTTCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACAt                                                                                  >  1:333078/1‑150 (MQ=255)
                                                                  tgttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACAt                                                                                  >  1:954576/1‑150 (MQ=255)
                                                                   gttatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACACTTTGCTGCAATTTGCAGCAACAACCATGAGTGTGTTATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATg                                                                                 <  2:127176/150‑1 (MQ=255)
                                                                     tatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTc                                                                               >  2:145533/1‑150 (MQ=255)
                                                                     tatgtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTc                                                                               <  2:1378124/150‑1 (MQ=255)
                                                                     tatgtAAATTGGTAAAACATTGTTGCGATGAATGTCAGATCCTCTGATCAATAACCATCGATTACCCTTTGCTGGAATTTGCTGAAAGAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTAGACATGTc                                                                               <  2:1335885/150‑1 (MQ=255)
                                                                        gtAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTa                                                                            <  2:249339/150‑1 (MQ=255)
                                                                         tAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTAc                                                                           <  2:805802/150‑1 (MQ=255)
                                                                         tAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGACGGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTGGACATGTCTTAc                                                                           <  1:529837/150‑1 (MQ=255)
                                                                           aaTTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTCGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCa                                                                         <  2:630346/150‑1 (MQ=255)
                                                                               ggTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAg                                                                     >  1:666807/1‑150 (MQ=255)
                                                                                gTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAgg                                                                    >  1:637479/1‑150 (MQ=255)
                                                                                gTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAgg                                                                    <  1:1703255/150‑1 (MQ=255)
                                                                                  cAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTa                                                                  >  1:748336/1‑150 (MQ=255)
                                                                                  cAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTa                                                                  >  1:742038/1‑150 (MQ=255)
                                                                                   aaCCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAg                                                                 <  1:1398211/150‑1 (MQ=255)
                                                                                       aTTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACt                                                             <  2:1649228/150‑1 (MQ=255)
                                                                                              cGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGc                                                      >  2:448483/1‑150 (MQ=255)
                                                                                              cGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGc                                                      <  2:547038/150‑1 (MQ=255)
                                                                                                 tGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGAAATTTGCAGCAACAACCATGAGATTGAAATTCTTGTGATTTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCAt                                                   <  1:1020534/150‑1 (MQ=255)
                                                                                                     tGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCa                                               >  2:1337342/1‑150 (MQ=255)
                                                                                                     tGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCa                                               <  1:1632245/150‑1 (MQ=255)
                                                                                                     tGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCa                                               >  1:359690/1‑150 (MQ=255)
                                                                                                          caTCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGa                                          >  1:227813/1‑150 (MQ=255)
                                                                                                             ccTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGc                                       <  1:468291/150‑1 (MQ=255)
                                                                                                               tctGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCaa                                     <  2:302988/150‑1 (MQ=255)
                                                                                                                  gATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGc                                  >  1:489029/1‑150 (MQ=255)
                                                                                                                   aTCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGcc                                 >  2:331973/1‑150 (MQ=255)
                                                                                                                    tCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCa                                >  2:1210252/1‑150 (MQ=255)
                                                                                                                        tAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCAGCTCATCCGATGCAACGCCACGGc                            >  2:256154/1‑150 (MQ=255)
                                                                                                                          aCCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTg                          <  1:595171/150‑1 (MQ=255)
                                                                                                                             aTCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCgg                       >  1:1706617/1‑150 (MQ=255)
                                                                                                                               cGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGta                     >  2:612591/1‑149 (MQ=255)
                                                                                                                               cGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTc                     <  1:1318880/150‑1 (MQ=255)
                                                                                                                                  ttACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGTAACGCCACGGCTGCGGTCTgg                  <  2:581449/150‑1 (MQ=255)
                                                                                                                                  ttACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTgg                  <  2:893943/150‑1 (MQ=255)
                                                                                                                                  ttACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTgg                  <  1:575761/150‑1 (MQ=255)
                                                                                                                                    ggttgttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCAGATGCAACGCCACGGCTGCGGTCTgggt                >  1:869013/6‑148 (MQ=255)
                                                                                                                                     cccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTAACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttg               <  2:1027631/150‑1 (MQ=255)
                                                                                                                                     cccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATGCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttg               <  1:42026/150‑1 (MQ=255)
                                                                                                                                      ccTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAAGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgt              <  1:1200238/150‑1 (MQ=255)
                                                                                                                                       tgttGCTGCAAATTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgtt             >  2:1163659/3‑150 (MQ=255)
                                                                                                                                       cTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgtt             >  2:1258432/1‑150 (MQ=255)
                                                                                                                                       cTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgtt             <  1:292591/150‑1 (MQ=255)
                                                                                                                                       cTTTGCTGAAATTTTCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGgttgtt             <  2:25495/150‑1 (MQ=255)
                                                                                                                                        tttGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTgttccatg            >  1:421229/1‑143 (MQ=255)
                                                                                                                                        gttGCTGCAAATTGCAGCAACAACCATGAGAGTGATATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATAAGCCATCTCATCCGATGCAACGCCTCGGCTGCGGTCTGgttgtta            >  1:113938/2‑149 (MQ=255)
                                                                                                                                           gctgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATc         >  1:1177402/1‑150 (MQ=255)
                                                                                                                                           gctgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTAGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGTCTGCGGTCTGGTTGTTCATc         >  1:889401/1‑150 (MQ=255)
                                                                                                                                            ctgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATcc        >  1:1220063/1‑150 (MQ=255)
                                                                                                                                            ctgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATcc        >  1:1121697/1‑150 (MQ=255)
                                                                                                                                            ctgcAATTTGCAGCAACAACCATGAGAGTGAAATACTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATcc        >  2:1124435/1‑150 (MQ=255)
                                                                                                                                             tgcAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGATCACATGTCTTACCAAAAGGTAGAAGTTATACCCCATCTCATCCGATGCAACCCCACGGCTGCGGACTGCTTGATCATCCg       >  1:1568172/1‑150 (MQ=255)
                                                                                                                                              gcAATTTGCAGCAACAACCATGAGAGTGATATTCTAGTGATGTGGTTAACCAATTTCAGAATTCGGTTTGACATGTCTTACCAAAAGGTAGAACTTATACGACATCTCATCCGATTCAACGCCACGGCTGCGGTCTGGTTGTTCATCCgg      <  2:1120214/150‑1 (MQ=255)
                                                                                                                                                aaTTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGAt    <  2:1631481/150‑1 (MQ=255)
                                                                                                                                                  tttGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTTATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATAc  <  2:1484545/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GAGAATGTTAAGCACATGCATAACGTTTTACCTTACCTGGTTGAACCGTTGTTATTTTGGGCGATATGTTATGTAAATTGGTCAACCATTGTTGCGATGAATGTCACATCCTCTGATCAATAACCATCGATTACCCTTTGCTGCAATTTGCAGCAACAACCATGAGAGTGAAATTCTTGTGATGTGGTTAACCAATTTCAGAATTCGGGTTGACATGTCTTACCAAAAGGTAGAACTTATACGCCATCTCATCCGATGCAACGCCACGGCTGCGGTCTGGTTGTTCATCCGGATAC  >  NC_000913/4551276‑4551571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: