Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 643,511 G→A 6.0% intergenic (+185/+46) ybdR → / ← rnk putative Zn‑dependent NAD(P)‑binding oxidoreductase/regulator of nucleoside diphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913643,5110GA 6.0% 261.9 / 4.1 83intergenic (+185/+46)ybdR/rnkputative Zn‑dependent NAD(P)‑binding oxidoreductase/regulator of nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (45/33);  new base A (2/3);  total (47/36)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

ACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGAATCGCCA  >  NC_000913/643364‑643659
                                                                                                                                                   |                                                                                                                                                     
ccGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGGGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAg                                                                                                                                                     <  2:1360980/149‑1 (MQ=255)
 ctaTAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGt                                                                                                                                                    <  2:38003/148‑1 (MQ=255)
     aaTCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCAt                                                                                                                                                <  1:1068868/150‑1 (MQ=255)
       tctccgccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATcc                                                                                                                                              <  1:964373/147‑1 (MQ=255)
         gccgccATAATGAGAAATGCTATGATTATTTAAAATGCTTCTGCCATTGTTATTCCAACAAAAGTAACGGAATAATTTTGCAGCAAAAGTGAACTGTGTGAACCAGGAAAAATTGCTGATTTTGCGTATAGAGGATGCACTCGCATCctc                                                                                                                                            <  2:517999/150‑1 (MQ=255)
         gccgccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCctc                                                                                                                                            >  1:1490225/1‑150 (MQ=255)
          ccgccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCTTAAATAGGATGCGATTGAATCctct                                                                                                                                           >  1:312530/1‑150 (MQ=255)
          ccgccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCctct                                                                                                                                           >  1:968891/1‑150 (MQ=255)
            gccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCACTCGCATCctcttt                                                                                                                                         <  2:50702/150‑3 (MQ=255)
             ccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTggg                                                                                                                                        <  1:949244/150‑1 (MQ=255)
             ccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTggg                                                                                                                                        <  1:1046483/150‑1 (MQ=255)
              cATAATAAGAAATCCTATAAGGATGTAAAATGCTTCTGCCATGGTTATTCCAACAAACGAAACGTAATAATTTTGCAGCATAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATTCACTCGCATCctctttac                                                                                                                                       <  2:1080022/150‑5 (MQ=255)
                 aaTCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCaaa                                                                                                                                    >  1:303833/1‑150 (MQ=255)
                  aTCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAg                                                                                                                                   >  2:1131887/1‑150 (MQ=255)
                  aTCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAg                                                                                                                                   >  2:1125951/1‑150 (MQ=255)
                   taaGAAATCCTATCAGGATGTAAAATGATTCTGCCATGTTTATTCCCACAAACGAAACGGAAAAATTTTGCAGCAAAATGCAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGc                                                                                                                                  <  2:120651/148‑1 (MQ=255)
                    cAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGAATCCTCTGGGCaaatcc                                                                                                                                 >  2:236554/1‑147 (MQ=255)
                      gAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAg                                                                                                                               >  2:1548225/1‑150 (MQ=255)
                        aaTCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGtt                                                                                                                             >  2:1281515/1‑150 (MQ=255)
                            cTATCAGGATGTAAAAAGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCg                                                                                                                         <  2:659510/150‑1 (MQ=255)
                                cAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTtg                                                                                                                     <  2:1490225/150‑1 (MQ=255)
                                aagtaTGTAATATGCTTCTGCCATCGTTATTCCCACAAACGAAACGGATTAATTTTGCAGCAAAGTGTACAGTGAGAACCAGGTAAAATTGCTGTTTTTGCGTATAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTtgt                                                                                                                    <  1:206764/146‑1 (MQ=255)
                                   gATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGc                                                                                                                  <  2:1294594/150‑1 (MQ=255)
                                     tGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAACAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCATCTGGGCAAAGCGATTTATCGCTTGTGCAg                                                                                                                >  2:210582/1‑150 (MQ=255)
                                        aaaaTGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATg                                                                                                             >  2:105012/1‑150 (MQ=255)
                                                 cTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAg                                                                                                   <  2:590823/150‑1 (MQ=255)
                                                 cTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAg                                                                                                   >  2:200371/1‑150 (MQ=255)
                                                  tGCCATGTTTATTCCCACAAACGAAACGGAATAATTTTGCATCAAAAGTGAACAGAGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGc                                                                                                  <  2:310140/150‑1 (MQ=255)
                                                  tGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGc                                                                                                  <  1:778906/150‑1 (MQ=255)
                                                   gCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCa                                                                                                 >  2:1376288/1‑150 (MQ=255)
                                                      aTGGTTATTCCCACAACCGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGt                                                                                              <  2:338837/150‑1 (MQ=255)
                                                          ttATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTc                                                                                          >  1:1308094/1‑150 (MQ=255)
                                                             ttCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGcc                                                                                       >  1:178236/1‑150 (MQ=255)
                                                               cccACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCcac                                                                                     >  2:512566/1‑149 (MQ=255)
                                                                ccACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCcagc                                                                                    >  2:371011/1‑150 (MQ=255)
                                                                ccACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCcagc                                                                                    <  2:448435/150‑1 (MQ=255)
                                                                 cacaAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCTTAAAGAGGATGCGAGTGCATCCTCTGGGCATAGCGAGTTATCGCTTGTGCAGAGGGGTTTAAAGCAGGTAGTCGCcagca                                                                                   >  2:252384/1‑150 (MQ=255)
                                                                 cacaAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAACGCAGGTAGTCGCcagca                                                                                   >  2:213634/1‑150 (MQ=255)
                                                                 cacaAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCcagca                                                                                   >  2:1444103/1‑150 (MQ=255)
                                                                         aaacgGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTctgg                                                                           >  2:337723/1‑150 (MQ=255)
                                                                             ggAATACTTTTGCAGCAAAAGTGAACAGTGACAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTAATCGCTTGTGCAGATGGGATTAAAGCAGGAAGTCGCCAGCAGCTTctggctgg                                                                       <  1:840997/150‑1 (MQ=255)
                                                                             ggAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTctggctgg                                                                       <  1:1131887/150‑1 (MQ=255)
                                                                             ggAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTctggctgg                                                                       <  1:1125951/150‑1 (MQ=255)
                                                                               aataatTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTa                                                                     <  1:236554/150‑1 (MQ=255)
                                                                                     tttGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAg                                                               <  2:1649145/150‑1 (MQ=255)
                                                                                        gcagcaAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTc                                                            >  2:1452813/1‑150 (MQ=255)
                                                                                        gcagcaAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTc                                                            >  1:554914/1‑150 (MQ=255)
                                                                                        gcagcaAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTc                                                            <  1:372994/150‑1 (MQ=255)
                                                                                           gcaAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAg                                                         >  2:1569877/1‑150 (MQ=255)
                                                                                           gcaAAAGTGAACAGTGAGAACAAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTATAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAg                                                         >  2:858484/1‑150 (MQ=255)
                                                                                             aaaaGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCa                                                       >  1:590836/1‑150 (MQ=255)
                                                                                               attttatcAGTGAGATCAAGGAAAAATTGCTGATTTTTCGTATAGTTGATGCGAGTGCATCCTCTTGGAAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAATCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACt                                                     <  2:1596799/143‑1 (MQ=255)
                                                                                                aGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACtt                                                    <  1:57340/150‑1 (MQ=255)
                                                                                                 gTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTc                                                   <  1:1496346/150‑1 (MQ=255)
                                                                                                 gTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTc                                                   >  1:9616/1‑150 (MQ=255)
                                                                                                    aaCAGTGAGAAACACTAAAAATTGCTGTTTTTGCCTAAAGAGGAAGCGAGTGCATCCTCTTGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAATCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAg                                                <  2:1570410/150‑1 (MQ=255)
                                                                                                      cAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGt                                              >  2:1512082/1‑150 (MQ=255)
                                                                                                          gagaACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGt                                          >  1:846380/1‑150 (MQ=255)
                                                                                                           agaACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGtt                                         >  2:1134457/1‑150 (MQ=255)
                                                                                                            gaACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTg                                        >  1:889272/1‑150 (MQ=255)
                                                                                                               ccAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCaa                                     >  2:1317903/1‑150 (MQ=255)
                                                                                                               ccAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCaa                                     >  2:1434498/1‑150 (MQ=255)
                                                                                                                catgAAAAATTGATGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAAc                                    <  2:876243/147‑1 (MQ=255)
                                                                                                                 aGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTACATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAAcg                                   >  1:895808/1‑150 (MQ=255)
                                                                                                                  ggAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAAcgc                                  >  2:1309044/1‑150 (MQ=255)
                                                                                                                    aaaaaTTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAAcgccg                                >  1:144614/1‑150 (MQ=255)
                                                                                                                      aaaTTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACgccgcc                              <  1:1548225/150‑1 (MQ=255)
                                                                                                                          tGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGa                          >  1:350131/1‑150 (MQ=255)
                                                                                                                             tGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGt                       <  2:846380/150‑1 (MQ=255)
                                                                                                                               aTTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTc                     <  1:200371/150‑1 (MQ=255)
                                                                                                                                     cGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCTGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTg               <  2:1509863/150‑1 (MQ=255)
                                                                                                                                     cGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTg               >  2:1291205/1‑150 (MQ=255)
                                                                                                                                     cGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTg               <  2:1517207/150‑1 (MQ=255)
                                                                                                                                      gTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGa              >  2:369673/1‑150 (MQ=255)
                                                                                                                                      gTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGa              >  2:200144/1‑150 (MQ=255)
                                                                                                                                      gTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGa              >  1:816888/1‑150 (MQ=255)
                                                                                                                                       cccagagGATGCACTCGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCCGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGaa             >  1:485881/4‑150 (MQ=255)
                                                                                                                                        aaaaAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAAt            >  2:942196/1‑150 (MQ=255)
                                                                                                                                          agagGATGCACTCGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATgg          >  2:682996/1‑150 (MQ=255)
                                                                                                                                             ggATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGaa        >  2:983849/1‑150 (MQ=255)
                                                                                                                                             ggATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGAAt       >  1:298395/1‑150 (MQ=255)
                                                                                                                                              gATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCGCAGTGAATGGAATc      >  1:181511/1‑150 (MQ=255)
                                                                                                                                                  cGAGTGCATCCTCTGGGCAAAGCGAGGTATCGGTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGAATCGCCa  <  1:105012/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGAATCGCCA  >  NC_000913/643364‑643659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: