Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 643,514 T→C 6.0% intergenic (+188/+43) ybdR → / ← rnk putative Zn‑dependent NAD(P)‑binding oxidoreductase/regulator of nucleoside diphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913643,5140TC 6.0% 237.6 / 3.7 82intergenic (+188/+43)ybdR/rnkputative Zn‑dependent NAD(P)‑binding oxidoreductase/regulator of nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base T (45/32);  new base C (2/3);  total (47/35)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACG  >  NC_000913/643365‑643662
                                                                                                                                                     |                                                                                                                                                     
ctaTAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGt                                                                                                                                                       <  2:38003/148‑1 (MQ=255)
    aaTCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCAt                                                                                                                                                   <  1:1068868/150‑1 (MQ=255)
      tctccgccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATcc                                                                                                                                                 <  1:964373/147‑1 (MQ=255)
        gccgccATAATGAGAAATGCTATGATTATTTAAAATGCTTCTGCCATTGTTATTCCAACAAAAGTAACGGAATAATTTTGCAGCAAAAGTGAACTGTGTGAACCAGGAAAAATTGCTGATTTTGCGTATAGAGGATGCACTCGCATCctc                                                                                                                                               <  2:517999/150‑1 (MQ=255)
        gccgccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCctc                                                                                                                                               >  1:1490225/1‑150 (MQ=255)
         ccgccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCTTAAATAGGATGCGATTGAATCctct                                                                                                                                              >  1:312530/1‑150 (MQ=255)
         ccgccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCctct                                                                                                                                              >  1:968891/1‑150 (MQ=255)
           gccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCACTCGCATCctcttt                                                                                                                                            <  2:50702/150‑3 (MQ=255)
            ccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTggg                                                                                                                                           <  1:1046483/150‑1 (MQ=255)
            ccATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTggg                                                                                                                                           <  1:949244/150‑1 (MQ=255)
             cATAATAAGAAATCCTATAAGGATGTAAAATGCTTCTGCCATGGTTATTCCAACAAACGAAACGTAATAATTTTGCAGCATAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATTCACTCGCATCctctttac                                                                                                                                          <  2:1080022/150‑5 (MQ=255)
                aaTCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCaaa                                                                                                                                       >  1:303833/1‑150 (MQ=255)
                 aTCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAg                                                                                                                                      >  2:1131887/1‑150 (MQ=255)
                 aTCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAg                                                                                                                                      >  2:1125951/1‑150 (MQ=255)
                  taaGAAATCCTATCAGGATGTAAAATGATTCTGCCATGTTTATTCCCACAAACGAAACGGAAAAATTTTGCAGCAAAATGCAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGc                                                                                                                                     <  2:120651/148‑1 (MQ=255)
                   cAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGAATCCTCTGGGCaaatcc                                                                                                                                    >  2:236554/1‑147 (MQ=255)
                     gAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAg                                                                                                                                  >  2:1548225/1‑150 (MQ=255)
                       aaTCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGtt                                                                                                                                >  2:1281515/1‑150 (MQ=255)
                           cTATCAGGATGTAAAAAGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCg                                                                                                                            <  2:659510/150‑1 (MQ=255)
                               cAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTtg                                                                                                                        <  2:1490225/150‑1 (MQ=255)
                               aagtaTGTAATATGCTTCTGCCATCGTTATTCCCACAAACGAAACGGATTAATTTTGCAGCAAAGTGTACAGTGAGAACCAGGTAAAATTGCTGTTTTTGCGTATAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTtgt                                                                                                                       <  1:206764/146‑1 (MQ=255)
                                  gATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGc                                                                                                                     <  2:1294594/150‑1 (MQ=255)
                                    tGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAACAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCATCTGGGCAAAGCGATTTATCGCTTGTGCAg                                                                                                                   >  2:210582/1‑150 (MQ=255)
                                       aaaaTGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATg                                                                                                                >  2:105012/1‑150 (MQ=255)
                                                cTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAg                                                                                                      <  2:590823/150‑1 (MQ=255)
                                                cTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAg                                                                                                      >  2:200371/1‑150 (MQ=255)
                                                 tGCCATGTTTATTCCCACAAACGAAACGGAATAATTTTGCATCAAAAGTGAACAGAGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGc                                                                                                     <  2:310140/150‑1 (MQ=255)
                                                 tGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGc                                                                                                     <  1:778906/150‑1 (MQ=255)
                                                  gCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCa                                                                                                    >  2:1376288/1‑150 (MQ=255)
                                                     aTGGTTATTCCCACAACCGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGt                                                                                                 <  2:338837/150‑1 (MQ=255)
                                                         ttATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTc                                                                                             >  1:1308094/1‑150 (MQ=255)
                                                            ttCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGcc                                                                                          >  1:178236/1‑150 (MQ=255)
                                                              cccACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCcac                                                                                        >  2:512566/1‑149 (MQ=255)
                                                               ccACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCcagc                                                                                       >  2:371011/1‑150 (MQ=255)
                                                               ccACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCcagc                                                                                       <  2:448435/150‑1 (MQ=255)
                                                                cacaAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCTTAAAGAGGATGCGAGTGCATCCTCTGGGCATAGCGAGTTATCGCTTGTGCAGAGGGGTTTAAAGCAGGTAGTCGCcagca                                                                                      >  2:252384/1‑150 (MQ=255)
                                                                cacaAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAACGCAGGTAGTCGCcagca                                                                                      >  2:213634/1‑150 (MQ=255)
                                                                cacaAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCcagca                                                                                      >  2:1444103/1‑150 (MQ=255)
                                                                        aaacgGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTctgg                                                                              >  2:337723/1‑150 (MQ=255)
                                                                            ggAATACTTTTGCAGCAAAAGTGAACAGTGACAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTAATCGCTTGTGCAGATGGGATTAAAGCAGGAAGTCGCCAGCAGCTTctggctgg                                                                          <  1:840997/150‑1 (MQ=255)
                                                                            ggAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTctggctgg                                                                          <  1:1131887/150‑1 (MQ=255)
                                                                            ggAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTctggctgg                                                                          <  1:1125951/150‑1 (MQ=255)
                                                                              aataatTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTa                                                                        <  1:236554/150‑1 (MQ=255)
                                                                                    tttGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAg                                                                  <  2:1649145/150‑1 (MQ=255)
                                                                                       gcagcaAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTc                                                               >  1:554914/1‑150 (MQ=255)
                                                                                       gcagcaAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTc                                                               >  2:1452813/1‑150 (MQ=255)
                                                                                       gcagcaAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTc                                                               <  1:372994/150‑1 (MQ=255)
                                                                                          gcaAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAg                                                            >  2:1569877/1‑150 (MQ=255)
                                                                                          gcaAAAGTGAACAGTGAGAACAAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTATAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAg                                                            >  2:858484/1‑150 (MQ=255)
                                                                                            aaaaGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCa                                                          >  1:590836/1‑150 (MQ=255)
                                                                                              attttatcAGTGAGATCAAGGAAAAATTGCTGATTTTTCGTATAGTTGATGCGAGTGCATCCTCTTGGAAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAATCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACt                                                        <  2:1596799/143‑1 (MQ=255)
                                                                                               aGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACtt                                                       <  1:57340/150‑1 (MQ=255)
                                                                                                gTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTc                                                      >  1:9616/1‑150 (MQ=255)
                                                                                                gTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTc                                                      <  1:1496346/150‑1 (MQ=255)
                                                                                                   aaCAGTGAGAAACACTAAAAATTGCTGTTTTTGCCTAAAGAGGAAGCGAGTGCATCCTCTTGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAATCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAg                                                   <  2:1570410/150‑1 (MQ=255)
                                                                                                     cAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGt                                                 >  2:1512082/1‑150 (MQ=255)
                                                                                                         gagaACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGt                                             >  1:846380/1‑150 (MQ=255)
                                                                                                          agaACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGtt                                            >  2:1134457/1‑150 (MQ=255)
                                                                                                           gaACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTg                                           >  1:889272/1‑150 (MQ=255)
                                                                                                              ccAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCaa                                        >  2:1434498/1‑150 (MQ=255)
                                                                                                              ccAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCaa                                        >  2:1317903/1‑150 (MQ=255)
                                                                                                               catgAAAAATTGATGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAAc                                       <  2:876243/147‑1 (MQ=255)
                                                                                                                aGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTACATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAAcg                                      >  1:895808/1‑150 (MQ=255)
                                                                                                                 ggAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAAcgc                                     >  2:1309044/1‑150 (MQ=255)
                                                                                                                   aaaaaTTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAAcgccg                                   >  1:144614/1‑150 (MQ=255)
                                                                                                                     aaaTTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACgccgcc                                 <  1:1548225/150‑1 (MQ=255)
                                                                                                                         tGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGa                             >  1:350131/1‑150 (MQ=255)
                                                                                                                            tGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGt                          <  2:846380/150‑1 (MQ=255)
                                                                                                                              aTTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTc                        <  1:200371/150‑1 (MQ=255)
                                                                                                                                    cGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCTGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTg                  <  2:1509863/150‑1 (MQ=255)
                                                                                                                                    cGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTg                  <  2:1517207/150‑1 (MQ=255)
                                                                                                                                    cGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTg                  >  2:1291205/1‑150 (MQ=255)
                                                                                                                                     gTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGa                 >  1:816888/1‑150 (MQ=255)
                                                                                                                                     gTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGa                 >  2:369673/1‑150 (MQ=255)
                                                                                                                                     gTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGa                 >  2:200144/1‑150 (MQ=255)
                                                                                                                                      cccagagGATGCACTCGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCCGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGaa                >  1:485881/4‑150 (MQ=255)
                                                                                                                                       aaaaAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAAt               >  2:942196/1‑150 (MQ=255)
                                                                                                                                         agagGATGCACTCGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATgg             >  2:682996/1‑150 (MQ=255)
                                                                                                                                            ggATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGaa           >  2:983849/1‑150 (MQ=255)
                                                                                                                                            ggATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGAAt          >  1:298395/1‑150 (MQ=255)
                                                                                                                                             gATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCGCAGTGAATGGAATc         >  1:181511/1‑150 (MQ=255)
                                                                                                                                                 cGAGTGCATCCTCTGGGCAAAGCGAGGTATCGGTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGAATCGCCa     <  1:105012/150‑1 (MQ=255)
                                                                                                                                                    gTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGAATCGCCAAcg  <  2:554914/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGATAATCGCCGCCATAATCAGAAATCCTATCAGGATGTAAAATGCTTCTGCCATGGTTATTCCCACAAACGAAACGGAATAATTTTGCAGCAAAAGTGAACAGTGAGAACCAGGAAAAATTGCTGATTTTGCGTAAAGAGGATGCGAGTGCATCCTCTGGGCAAAGCGAGTTATCGCTTGTGCAGATGGGATTAAAGCAGGTAGTCGCCAGCAGCTTCTGGCTGGTATTCGAGTTCCAGCACTTCAAGGTGGGTTGCAACGCCGCCCGGAAGTTCCCAGTGAATGGAATCGCCAACG  >  NC_000913/643365‑643662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: