Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 644,034 T→C 5.1% intergenic (‑67/+163) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913644,0340TC 5.1% 469.4 / 10.8 157intergenic (‑67/+163)rnk/rnaregulator of nucleoside diphosphate kinase/ribonuclease I
Reads supporting (aligned to +/- strand):  ref base T (79/70);  new base C (4/4);  total (83/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-02

Only 100 of 157 total aligned reads displayed.

CCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTT  >  NC_000913/643888‑644182
                                                                                                                                                  |                                                                                                                                                    
ccAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAg                                                                                                                                                   >  2:787427/1‑150 (MQ=255)
ccAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAg                                                                                                                                                   >  2:1008219/1‑150 (MQ=255)
        ggCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAg                                                                                                                                           <  1:905697/150‑1 (MQ=255)
        ggCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTAGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAg                                                                                                                                           >  1:1726209/1‑150 (MQ=255)
          cGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAggg                                                                                                                                         <  1:370628/150‑1 (MQ=255)
          cGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGGCGTTAATGATGATAGTTGGGCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAggg                                                                                                                                         <  1:1121783/150‑1 (MQ=255)
           gggTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGGCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCGCCGCCTGACGGTGAGGGt                                                                                                                                        <  2:1139983/150‑1 (MQ=255)
            ggTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGtt                                                                                                                                       >  2:1647694/1‑150 (MQ=255)
            ggTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGtt                                                                                                                                       >  1:696099/1‑150 (MQ=255)
             gTTGCGCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGGCTGGACATTTTTACTCCATGGCGGCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGttt                                                                                                                                      <  2:1080799/150‑1 (MQ=255)
             gTTGCGCCAGCAGAATATCGATGCGTTCGGCATCCAGGGCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCGCCGCCTGACGGTGAGGGttt                                                                                                                                      <  1:868007/150‑1 (MQ=255)
              ttGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTa                                                                                                                                     <  2:855266/150‑1 (MQ=255)
              ttGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGATCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTa                                                                                                                                     <  2:1022297/150‑1 (MQ=255)
                gCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAAc                                                                                                                                   <  2:9110/150‑1 (MQ=255)
                gCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAAACATCACCGTCAGGCGGCGAGGGTTTaaa                                                                                                                                   >  2:1577675/1‑149 (MQ=255)
                 cTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGCGGGTTTAACt                                                                                                                                  >  1:1570037/1‑150 (MQ=255)
                 cTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACt                                                                                                                                  >  1:1646870/1‑150 (MQ=255)
                   ccAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTca                                                                                                                                <  1:1029567/150‑1 (MQ=255)
                   ccAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTca                                                                                                                                >  1:700730/1‑150 (MQ=255)
                   ccAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTca                                                                                                                                >  1:208441/1‑150 (MQ=255)
                   ccAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTca                                                                                                                                >  2:1412720/1‑150 (MQ=255)
                    cagcagAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTcac                                                                                                                               <  1:786840/150‑1 (MQ=255)
                      gcagAATATCGATGCGTTCGTCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACat                                                                                                                             <  2:683512/150‑1 (MQ=255)
                      gcagAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACat                                                                                                                             >  2:320601/1‑150 (MQ=255)
                        agAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACatga                                                                                                                           >  1:633144/1‑150 (MQ=255)
                            tatCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATgatgat                                                                                                                       >  2:105276/1‑150 (MQ=255)
                               cGATGCGTTCGGCATCCAGGTCGTTAATGCTGATAGTTGGTCTGGAGATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCTGATAAACATACCAAAAAGAAAACCCTAACCGTCAGGCGGCGAGGGTTTAACTCACATGAGGATact                                                                                                                    >  2:284174/1‑150 (MQ=255)
                                gATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAGAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATactg                                                                                                                   >  1:1080805/1‑150 (MQ=255)
                                gATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATactg                                                                                                                   <  2:606248/150‑1 (MQ=255)
                                gATGAGTGCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATactg                                                                                                                   <  2:1460123/150‑1 (MQ=255)
                                  tGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATactgac                                                                                                                 <  2:1622735/150‑1 (MQ=255)
                                     gTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGt                                                                                                              >  1:894351/1‑150 (MQ=255)
                                               cAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCActct                                                                                                    >  2:404994/1‑150 (MQ=255)
                                                aGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCtt                                                                                                   >  1:538805/1‑150 (MQ=255)
                                                  ggcGTTACAGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGGCGGGGCTGCGAGTGGCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTg                                                                                                 <  2:282797/148‑1 (MQ=255)
                                                  gTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTg                                                                                                 <  1:1004074/150‑1 (MQ=255)
                                                  gTCGTTAATGATGATAGTTGGGCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCTAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTg                                                                                                 <  2:86314/150‑1 (MQ=255)
                                                   tCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGa                                                                                                <  1:1639874/150‑1 (MQ=255)
                                                    cGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACGAAAAAGAAAACCATCACCGTCAGGCGGGGGGGGTTTATCTCGCATGATGATTCTGTCTGGTGCTCACTCGTTGaa                                                                                               >  1:1120587/1‑150 (MQ=255)
                                                    cGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGTGGGTTTAACTCACATGATGATACTGCCTGTTGCTCACTGTTTgta                                                                                               >  1:1126304/1‑148 (MQ=255)
                                                    cGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGaa                                                                                               >  1:1705905/1‑150 (MQ=255)
                                                     gTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAg                                                                                              <  1:649253/150‑1 (MQ=255)
                                                      ttAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGt                                                                                             <  2:468482/150‑1 (MQ=255)
                                                       tAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTg                                                                                            >  2:808538/1‑150 (MQ=255)
                                                       tAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTg                                                                                            >  1:498473/1‑150 (MQ=255)
                                                       tAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTg                                                                                            >  1:505052/1‑150 (MQ=255)
                                                        aaTGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGa                                                                                           >  1:969250/1‑150 (MQ=255)
                                                         attatgatAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGAt                                                                                          <  2:1329189/147‑1 (MQ=255)
                                                          tgatgaTAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGAtt                                                                                         <  2:939715/150‑1 (MQ=255)
                                                           gatgatAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGAttt                                                                                        >  2:359370/1‑150 (MQ=255)
                                                           gatgatAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATAATGACTGTTGCTCACTCTTTGAAGTGAttt                                                                                        >  1:551776/1‑150 (MQ=255)
                                                            atgatAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTg                                                                                       >  1:145672/1‑150 (MQ=255)
                                                              gatAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCg                                                                                     <  1:1652630/150‑1 (MQ=255)
                                                              gatAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTAAGGCGGCGAGGGTTTAACTGAAATGATCATACTGACTGTTGCTCACTCGTTGAATTGATTTGCg                                                                                     >  2:1132786/1‑150 (MQ=255)
                                                                tAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTc                                                                                   <  2:784929/150‑1 (MQ=255)
                                                                aaGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTc                                                                                   <  2:844649/149‑1 (MQ=255)
                                                                 aGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTca                                                                                  >  2:6250/1‑150 (MQ=255)
                                                                  tttGGTATGTACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTcac                                                                                 <  1:310252/149‑1 (MQ=255)
                                                                  gTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTcac                                                                                 <  2:1178976/150‑1 (MQ=255)
                                                                        cTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAg                                                                           >  2:1655826/1‑150 (MQ=255)
                                                                         tGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAgg                                                                          <  1:1170405/150‑1 (MQ=255)
                                                                          ggACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAggg                                                                         >  2:1565031/1‑150 (MQ=255)
                                                                             aaTTTTTACTCCATGTCGTCGTTGCTGCGAGTGTCGGAGATAAACTTACCCAAAAGAAAACCCTCTCCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAAt                                                                      <  1:968726/149‑1 (MQ=255)
                                                                               tttttACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGTGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTc                                                                    <  1:1239412/150‑1 (MQ=255)
                                                                                ttttACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTcc                                                                   >  2:597357/1‑150 (MQ=255)
                                                                                ttttACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTcc                                                                   >  2:435028/1‑150 (MQ=255)
                                                                                  ttACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTc                                                                 <  1:993579/150‑1 (MQ=255)
                                                                                  ttACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTc                                                                 <  1:320601/150‑1 (MQ=255)
                                                                                  ttACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTc                                                                 <  1:1393433/150‑1 (MQ=255)
                                                                                   tACTCCATGTCGTCTGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCa                                                                <  1:490378/150‑1 (MQ=255)
                                                                                    aCTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCaa                                                               <  1:1688659/150‑1 (MQ=255)
                                                                                       ccATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTACTCAATGc                                                            >  2:488187/1‑150 (MQ=255)
                                                                                         aTGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAg                                                          >  2:1470543/1‑150 (MQ=255)
                                                                                          tGTCGTAGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAACCCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAgc                                                         <  2:1380837/150‑1 (MQ=255)
                                                                                           gtcgtcGGTGCTGCGAGTGTCGCAGATAAACATACACAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTATGAAGTGATTTGCTTCACATTAAGGGAATTCCTCAATGCAgcg                                                        >  2:243980/1‑150 (MQ=255)
                                                                                             cgtcgGTGCTGCGAGGGTCGCAGATAAACATACCCAAAAGAAACCCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGCAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCa                                                      <  2:1067199/150‑1 (MQ=255)
                                                                                                cgGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTa                                                   <  1:1711651/150‑1 (MQ=255)
                                                                                                   tgctgcGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGt                                                <  2:1570037/150‑1 (MQ=255)
                                                                                                    gctgcGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGta                                               >  1:1131009/1‑150 (MQ=255)
                                                                                                    gctgcGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGta                                               <  2:509436/150‑1 (MQ=255)
                                                                                                       gcGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATaa                                            >  2:141191/1‑150 (MQ=255)
                                                                                                         gAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGTAATTCCTCAATGCAGCGCATTATGTATAAAt                                          <  2:773147/150‑1 (MQ=255)
                                                                                                           gtgtCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCt                                        <  2:179535/150‑1 (MQ=255)
                                                                                                                  aGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGc                                 <  2:1014792/150‑1 (MQ=255)
                                                                                                                  aGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGc                                 >  1:1569953/1‑150 (MQ=255)
                                                                                                                         cATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGtt                          <  2:1131009/150‑1 (MQ=255)
                                                                                                                            aCCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTAt                       <  1:1470543/150‑1 (MQ=255)
                                                                                                                             cccAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATg                      >  2:1585657/1‑150 (MQ=255)
                                                                                                                             cacAAAAGAAAACCCTCACCGTCAGGAGGCGAGGGTTTTAGTGACATAATTCTACTGACTGTTGGTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGATTTCCTCAATGCAGAGCATTATGTATTAATCTTAATCGCCTTGGTTTATg                      >  2:981459/3‑150 (MQ=255)
                                                                                                                              ccAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATgg                     >  2:744771/1‑150 (MQ=255)
                                                                                                                              ccAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATgg                     <  2:208441/150‑1 (MQ=255)
                                                                                                                               cAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGAAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGa                    >  2:496986/1‑150 (MQ=255)
                                                                                                                                 gagttaaaCCCTCGCCGCCTGACGGTGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGAGTTGCGTCACATTCAGGGAATGCCTCAATGCAGCGCAGTAGGGAGGATTCGGAATCGCCGTGGTTTATGGAAg                  >  2:1087628/6‑150 (MQ=255)
                                                                                                                                   gttaaaCCCTCGCCGCCTGACGGTGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGAc                >  2:686753/4‑150 (MQ=255)
                                                                                                                                   aGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGAc                >  2:866152/1‑150 (MQ=255)
                                                                                                                                   aGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGAc                <  1:1412720/150‑1 (MQ=255)
                                                                                                                                     aaaaCCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGa              >  2:418349/1‑150 (MQ=255)
                                                                                                                                          ccTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTAACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTAGGTTTATGGAAGACGAATTAg         >  1:820334/1‑150 (MQ=255)
                                                                                                                                            tCACCGTCAGTCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCg       <  2:1287991/150‑1 (MQ=255)
                                                                                                                                                 gTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGttt  <  1:554040/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CCAGCATAGGCGGGTTGCTCCAGCAGAATATCGATGCGTTCGGCATCCAGGTCGTTAATGATGATAGTTGGTCTGGACATTTTTACTCCATGTCGTCGGTGCTGCGAGTGTCGCAGATAAACATACCCAAAAGAAAACCCTCACCGTCAGGCGGCGAGGGTTTAACTCACATGATGATACTGACTGTTGCTCACTCTTTGAAGTGATTTGCGTCACATTCAGGGAATTCCTCAATGCAGCGCATTATGTATAAATCTTAATCGCCTTGGTTTATGGAAGACGAATTAGCGTGTTT  >  NC_000913/643888‑644182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: