| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 657,531 | T→G | 5.9% | intergenic (+30/+24) | cspE → / ← flc | constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 657,531 | 0 | T | G | 5.9% | 277.4 / 7.6 | 103 | intergenic (+30/+24) | cspE/flc | constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (41/56); new base G (3/3); total (44/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.19e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
GTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCAC > NC_000913/657382‑657680 | gTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCtt < 1:415152/150‑1 (MQ=255) cacTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAt > 2:79788/1‑150 (MQ=255) cTTCTCTGCAATCCAGACTAATGGTGTTAAAACTCTTGCTGAATGTCAGCGCGTAGAGTTCTAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa < 1:865757/150‑1 (MQ=255) tctcTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGc < 1:1321421/150‑1 (MQ=255) ctctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg > 1:361724/1‑150 (MQ=255) tctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAAAGGTGCCAAAGGACCTTCTGCTGCAAACGTAATCGCTATGTAAGAAACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCgg > 1:100639/1‑150 (MQ=255) ctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg > 1:816155/1‑150 (MQ=255) aaTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑‑tttt < 2:649651/150‑1 (MQ=255) aTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑‑ttttg < 2:591899/150‑2 (MQ=255) aTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGG‑‑ttttg < 1:1679641/150‑2 (MQ=255) ccAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCTCTTAATCAGCGGG‑‑ttttttt > 2:674804/1‑150 (MQ=255) ccAGACTAATGGTTTAAAAACTCATGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAATGCCCTTCTGCTGCAAACGTAATCGCTCAGTAAGATACGTCAGAAAGAATTCAAAACCCTCTTAATCAGCGGG‑‑ttttttt > 2:854712/1‑150 (MQ=255) cAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑tttttttt > 1:536181/1‑150 (MQ=255) aGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTg < 1:709187/150‑1 (MQ=255) taCTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAATCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTgg < 1:197788/149‑1 (MQ=255) gACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTCCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTgg < 2:208016/150‑1 (MQ=255) gACTAATGGTTTTAAAACTCTTGCTGAAGGGCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTgg < 1:1478823/150‑1 (MQ=255) ggTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAg < 2:573757/150‑1 (MQ=255) aaaaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTCACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCg < 1:864252/150‑1 (MQ=255) aaaaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCg < 1:1188282/150‑1 (MQ=255) aaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGt < 1:244805/150‑1 (MQ=255) aCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTATGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGtt < 2:1623260/150‑1 (MQ=255) ctctTGCTGAAGGTCAGCGCGAAGAGTTCGAAATCACT‑ACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGTATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGa < 1:239034/150‑1 (MQ=255) atctTGCTTAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGAGCCAAAGGCCCTTCTTCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTg < 2:1490116/149‑1 (MQ=255) cTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCg > 1:263614/1‑150 (MQ=255) tGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGa > 1:815066/1‑150 (MQ=255) gAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaa > 1:914338/1‑150 (MQ=255) aaGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaa < 1:744181/150‑1 (MQ=255) aGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaaaa < 1:1153626/150‑1 (MQ=255) tCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAg < 2:813523/150‑1 (MQ=255) aGCGCTTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTATGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTATTCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc < 2:1072550/150‑1 (MQ=255) aGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCAGCTTAATCAGCTGG‑‑TTTTTTTTGGTCTTTAGTGTGAGGTTGAGGCAGAAAACAGcc > 1:1098702/1‑150 (MQ=255) aGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCGCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑ATTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc > 1:1104825/1‑150 (MQ=255) ggcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAg < 1:428692/149‑1 (MQ=255) cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAg < 1:410902/150‑1 (MQ=255) gcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCTCTCTGTAAGATACGTCAGCAAGATTTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCAGAAAACAGCCAGa > 1:1623008/1‑150 (MQ=255) gTAGAGTTCGAAATCACTAACGTTGCCACAGGCCCTTCTGCTGCAACCGTACTCGCTCTGAAAGATACGTCTGCAAGAATTCAAAACCCGCTTAATCTGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAAt < 1:281064/150‑1 (MQ=255) gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg < 1:79788/150‑1 (MQ=255) cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGc < 1:1570402/150‑1 (MQ=255) cGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGAAAGAATTAAAAACCCGCTTTATCTGGGGGGTTTTTTTTTGGTCTTTTGTTGCCGGTTGAGGCCCAAAACCGCCAGAATGccgga > 2:85231/1‑147 (MQ=255) aaTCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCAGt > 2:759566/1‑150 (MQ=255) aCTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATg < 2:827768/150‑1 (MQ=255) cTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATgg > 1:800890/1‑150 (MQ=255) tAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc < 2:1378309/150‑1 (MQ=255) tAACGGTGCCAAACGCCCTTCTGCTGCAAACGTAATCGCTCTGAAAGATACGTCAGCAAGACTTCACAACCCGCTTACTCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc < 2:113852/150‑1 (MQ=255) ggTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa > 1:1716387/1‑150 (MQ=255) gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCATCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg < 2:1273327/150‑1 (MQ=255) gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg < 2:361724/150‑1 (MQ=255) gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg < 1:501977/150‑1 (MQ=255) gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAg < 2:206936/150‑1 (MQ=255) gCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAc > 1:314904/1‑150 (MQ=255) cAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGTTACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCTGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc < 1:1008765/150‑1 (MQ=255) cAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc > 2:480842/1‑150 (MQ=255) aGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAg > 2:64810/1‑150 (MQ=255) cccTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAAGCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAg < 2:263614/150‑1 (MQ=255) cTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGt > 2:662402/1‑150 (MQ=255) tCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTg < 1:607646/150‑1 (MQ=255) ctgctgCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGa < 2:1495984/150‑1 (MQ=255) ctgctgCAAACGTAATCGATCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGT‑TTTTTTTTGGGCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTg < 2:824267/150‑1 (MQ=255) gctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACg > 2:534063/1‑150 (MQ=255) ctgcAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGa > 1:926145/1‑150 (MQ=255) aaCGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg < 2:421948/150‑1 (MQ=255) gTAATCGCTTTGTAAGATACGTAAGCAAGAATTCAAAACCCGCTTAATCATCGTG‑‑TTTTTTTTGGTCTTTAGTTTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTc < 1:1016227/150‑1 (MQ=255) gTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTc < 2:1168782/150‑1 (MQ=255) gTAATCGCTCTGTAAGATACGTCAGAAAGATTTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTTGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTc < 2:342261/150‑1 (MQ=255) cGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTaa > 2:536416/1‑150 (MQ=255) gCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAAt > 1:458168/1‑150 (MQ=255) gCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAACGTTGACGAAAACGTTCAGTAAt > 1:1635501/1‑150 (MQ=255) ctctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATg < 2:380430/150‑1 (MQ=255) tctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGc < 2:911372/150‑1 (MQ=255) ctGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑GTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAACGACCACAGAAGGTTGACGAAAACGTTCAGTAATGcc > 1:1160711/1‑150 (MQ=255) tGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGccc < 2:536181/150‑1 (MQ=255) gATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCa > 1:518568/1‑150 (MQ=255) tACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGAGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTACAGTAATGCCCAGCCaaa > 1:427383/1‑150 (MQ=255) aCGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGACAAAAGACCCCAGAAGGTTGACGAACACGTTCAGTAATGCCCAGCCAAAg < 2:374875/150‑1 (MQ=255) cGTCAGCAAGAATTCAAAACCCGCATAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGc < 1:674804/150‑1 (MQ=255) cAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGc > 2:339453/1‑141 (MQ=255) cAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGc < 1:339453/141‑1 (MQ=255) aGCAATAATTAAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGGCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGcc < 1:854712/150‑1 (MQ=255) gCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TGTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAACGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCCGCCAAAGCGGccc > 1:1096635/1‑150 (MQ=255) cAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCct > 1:1536121/1‑150 (MQ=255) cAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCct > 1:1303057/1‑150 (MQ=255) aGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAACGCGGCCctct > 1:1331718/1‑150 (MQ=255) caaaaaaaaCCCGCTGATTAAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGAGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTg > 1:1707165/6‑150 (MQ=255) aaTTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTTATGCCCAGCCAAAGCGGCCCTCTTg < 2:1716387/150‑1 (MQ=255) aaTTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTg > 2:1138964/1‑150 (MQ=255) ttCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTa < 2:1473255/150‑1 (MQ=255) ttCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTa < 2:1102181/150‑1 (MQ=255) tCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTaa > 1:1074838/1‑150 (MQ=255) cAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGGCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAc < 1:1015121/150‑1 (MQ=255) aaaaaCCCGCTGATTAAGCGGG‑‑TATTTTTTGGTCTTTAGTGTACGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCACAGAAGGTTGACGAAAACGTTCAGTAATGCCAAGCCAAAGCGGCCCTCTTGTAAc > 1:845736/2‑150 (MQ=255) aaaaCCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACa < 2:694628/150‑1 (MQ=255) aaaaCCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACa < 2:926145/150‑1 (MQ=255) aaCCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaa > 1:1640327/1‑150 (MQ=255) aCCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGTCCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCGAGCCAAAGCGGCCCTCTTGTAACaaaa < 1:443426/150‑1 (MQ=255) cGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAaca > 1:1326090/1‑150 (MQ=255) gCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAacac < 1:1700792/150‑1 (MQ=255) gCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAacac > 1:730389/1‑150 (MQ=255) tAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcaccac > 2:4143/1‑150 (MQ=255) tAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcaccac > 1:925751/1‑150 (MQ=255) | GTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTGTAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGG‑‑TTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCAC > NC_000913/657382‑657680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |