Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 662,686 A→T 7.4% intergenic (‑44/+66) ybeD ← / ← dacA UPF0250 family protein/D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913662,6860AT 7.4% 326.2 / 10.2 121intergenic (‑44/+66)ybeD/dacAUPF0250 family protein/D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base A (62/49);  new base T (4/5);  total (67/54)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 123 total aligned reads displayed.

CTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACA  >  NC_000913/662543‑662834
                                                                                                                                               |                                                                                                                                                    
cTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCggg                                                                                                                                                <  2:1658975/150‑1 (MQ=255)
  tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAg                                                                                                                                              <  2:436008/150‑1 (MQ=255)
  tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAg                                                                                                                                              <  2:38731/150‑1 (MQ=255)
   cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGCAGGCGGGAGt                                                                                                                                             >  2:1302731/1‑150 (MQ=255)
   cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGt                                                                                                                                             >  2:793964/1‑150 (MQ=255)
   cAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTGTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGt                                                                                                                                             >  2:114710/1‑150 (MQ=255)
    aaCCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtt                                                                                                                                            >  1:1257687/1‑150 (MQ=255)
      ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTTCTGGCGGGAGttgc                                                                                                                                          <  1:87200/150‑3 (MQ=255)
      ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCTGCTGGCGGGAGttgc                                                                                                                                          <  2:1152494/150‑3 (MQ=255)
      ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtttt                                                                                                                                          <  1:1281181/150‑1 (MQ=255)
       caccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttttt                                                                                                                                         <  2:875084/150‑1 (MQ=255)
        accTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtttttt                                                                                                                                        >  2:1002621/1‑150 (MQ=255)
        accTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtttttt                                                                                                                                        >  2:536323/1‑150 (MQ=255)
         acTGATCAACCAGCTCAGGTAACGCCTGCGCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAATCATTTCGTTATGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTa                                                                                                                                       <  1:1069899/149‑1 (MQ=255)
           tGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACt                                                                                                                                     >  1:148754/1‑150 (MQ=255)
                   ccgCTCAGGTAACGCCGGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTa                                                                                                                             <  1:631979/148‑1 (MQ=255)
                    aGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAg                                                                                                                            >  1:8488/1‑150 (MQ=255)
                    aGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTAAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAg                                                                                                                            >  2:1218744/1‑150 (MQ=255)
                     gCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGt                                                                                                                           >  1:1609445/1‑150 (MQ=255)
                       tCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGtat                                                                                                                         >  2:139932/1‑150 (MQ=255)
                        cAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGtata                                                                                                                        >  2:751369/1‑150 (MQ=255)
                        cAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGtata                                                                                                                        >  1:448509/1‑150 (MQ=255)
                          ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATg                                                                                                                      >  1:653732/1‑150 (MQ=255)
                          ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCAAGAAGGCGGGAGTTTTTTACTGATGATTAGTATATg                                                                                                                      >  2:779106/1‑150 (MQ=255)
                          ggTAACGACTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTAAAGCAGTTCGTTAAGTTTTGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGCGGGCGTGAGTTTTTTACTCATGCTTAGTATATg                                                                                                                      >  1:610218/1‑150 (MQ=255)
                           gTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgg                                                                                                                     <  1:710300/150‑1 (MQ=255)
                            tAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATggg                                                                                                                    <  1:151068/150‑1 (MQ=255)
                                 ccTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATggggcagg                                                                                                               >  1:1495088/1‑146 (MQ=255)
                                    gCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGaaa                                                                                                            >  1:1551012/1‑150 (MQ=255)
                                     ccccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAAt                                                                                                           <  2:775043/150‑1 (MQ=255)
                                     ccccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAAt                                                                                                           <  1:806802/150‑1 (MQ=255)
                                       ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa                                                                                                         >  2:870767/1‑150 (MQ=255)
                                       ccATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTa                                                                                                         >  1:1044660/1‑150 (MQ=255)
                                         aTAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGGAATTATATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTAAATGGGGACGGAAATTaca                                                                                                       >  2:270968/1‑150 (MQ=255)
                                          tAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTacac                                                                                                      <  1:951356/150‑1 (MQ=255)
                                              tttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTc                                                                                                  <  1:830360/150‑1 (MQ=255)
                                                 gtaagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAg                                                                                               >  2:166200/1‑150 (MQ=255)
                                                 gtaagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAg                                                                                               <  2:1109426/150‑1 (MQ=255)
                                                 gtaagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAg                                                                                               <  2:1539874/150‑1 (MQ=255)
                                                   aattaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCCGATCCGGCGTAACGTAATTAAATAGCAAATCCCGCCAGAAGGCGGTAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAttg                                                                                             >  1:474112/4‑150 (MQ=255)
                                                   aagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAATTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAgtg                                                                                             <  2:205241/150‑1 (MQ=255)
                                                    agtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCACCAGGCGGGAGTATTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAgtgt                                                                                            >  1:1138648/1‑150 (MQ=255)
                                                     gtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGtt                                                                                           <  2:39354/150‑1 (MQ=255)
                                                            gAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAtttt                                                                                    <  2:849190/150‑1 (MQ=255)
                                                                aGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaac                                                                                <  2:1721204/150‑1 (MQ=255)
                                                                     aTTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaacc                                                                           >  1:506001/1‑148 (MQ=255)
                                                                     aTTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaac                                                                           >  1:1327367/1‑150 (MQ=255)
                                                                        cAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAg                                                                        >  2:1637909/1‑150 (MQ=255)
                                                                        cAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAg                                                                        >  2:1322150/1‑150 (MQ=255)
                                                                        cAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAg                                                                        >  1:1685844/1‑150 (MQ=255)
                                                                        cAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAg                                                                        <  2:8488/150‑1 (MQ=255)
                                                                        cAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAg                                                                        >  2:725024/1‑150 (MQ=255)
                                                                        cAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTACGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTATTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAAACAg                                                                        >  2:1690774/1‑150 (MQ=255)
                                                                            cAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGAt                                                                    <  1:518148/150‑1 (MQ=255)
                                                                             tgTTCGTAAAGTTTGGTGTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGCAGGGGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATg                                                                   <  2:225016/149‑1 (MQ=255)
                                                                                tCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGaa                                                                <  2:706209/150‑1 (MQ=255)
                                                                                  gTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACa                                                              <  1:751369/150‑1 (MQ=255)
                                                                                  gTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACa                                                              <  1:870767/150‑1 (MQ=255)
                                                                                    tAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAtt                                                            >  2:1733207/1‑150 (MQ=255)
                                                                                      aGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaa                                                          >  1:1020495/1‑150 (MQ=255)
                                                                                      aGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaa                                                          >  1:417380/1‑150 (MQ=255)
                                                                                         ttGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaattt                                                       <  2:122094/150‑1 (MQ=255)
                                                                                         ttGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaattt                                                       <  1:1475703/150‑1 (MQ=255)
                                                                                          tGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaattta                                                      <  2:406731/150‑1 (MQ=255)
                                                                                          tGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaattta                                                      >  1:211288/1‑150 (MQ=255)
                                                                                           tgTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatttaa                                                     <  1:725024/149‑1 (MQ=255)
                                                                                              tttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGt                                                  <  2:1153491/150‑1 (MQ=255)
                                                                                                 cATGTCAGCTCAGGCGTAAAGTAATAAAATAGCAACTCCCGCCAGAAGGAGGGAGTTTTTTACTGATTCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATAAATTTAATGTAAt                                               <  1:231369/150‑1 (MQ=255)
                                                                                                  aTGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATc                                              <  1:933000/150‑1 (MQ=255)
                                                                                                  aTGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGAGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATc                                              >  1:1064475/1‑150 (MQ=255)
                                                                                                    gTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCaa                                            >  1:686257/1‑150 (MQ=255)
                                                                                                     tCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAACCCAGTGATGGAACATTTAGTTAATGTAATCatt                                           >  2:150324/1‑148 (MQ=255)
                                                                                                     tCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAAt                                           >  1:16532/1‑150 (MQ=255)
                                                                                                          tCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGAttt                                      <  2:1609445/150‑1 (MQ=255)
                                                                                                             tgCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGc                                   <  1:424097/149‑1 (MQ=255)
                                                                                                                 taacgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGTGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATAAATGATTTTGCCgaa                               >  1:132284/1‑150 (MQ=255)
                                                                                                                  aacgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaag                              <  2:414845/150‑1 (MQ=255)
                                                                                                                   acgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTGATGTGTTTAATTTTTAACCTAACCAGTGAGGGAACCGTCATTTAATGTAAGCAATGATTTTGCCgaaga                             >  2:261139/1‑150 (MQ=255)
                                                                                                                    cgtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCgaagaa                            >  1:1513770/1‑150 (MQ=255)
                                                                                                                     gtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATTCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGAAGGAACATTAATTTAATGTAATCAATGATTTTGCCGaagaag                           >  1:1147912/1‑150 (MQ=255)
                                                                                                                     gtaaTTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGaagaag                           >  1:861782/1‑150 (MQ=255)
                                                                                                                         ttAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTAc                       <  1:166200/150‑1 (MQ=255)
                                                                                                                          tAAATAGCAACTCCCGCCAGAAGGCGGGAGTTATTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCAGAAGAAGTTAcc                      >  1:1411522/1‑150 (MQ=255)
                                                                                                                           aaaTAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCt                     >  2:652595/1‑150 (MQ=255)
                                                                                                                            aaTAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCtt                    >  2:1012566/1‑150 (MQ=255)
                                                                                                                            aaTAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGAAGCGTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGTCCATTAATTTAATGTAATCAATGATTATGCCGAAGAAGTTACCtt                    >  2:707642/1‑150 (MQ=255)
                                                                                                                               aaaaaCTCCCGCCTTCTGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATTTAATCAATGATTTTGCCGAAGAATTTACCTTcag                 >  1:1010331/4‑148 (MQ=255)
                                                                                                                               aaaaaCTCCCGCCTTCTGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCg                 >  1:1552259/4‑150 (MQ=255)
                                                                                                                                gCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCgg                >  1:562237/1‑150 (MQ=255)
                                                                                                                                aaaaCTCCCGCCTTCTGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCATGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCgg                >  1:1674846/3‑150 (MQ=255)
                                                                                                                                 cAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCggg               >  2:1356973/1‑150 (MQ=255)
                                                                                                                                   aCTCCCGCCTTCTGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAt             >  1:664076/1‑150 (MQ=255)
                                                                                                                                   aCTCCCGCCATAATGCTGGAGTTTTTTAATGATGATTAGTATATGTTGACTGAAATTACAATTTCATGTGTTTAATTTTTTACCAAACCAGTGATTTATCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAt             <  1:241729/150‑1 (MQ=255)
                                                                                                                                   aCTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAt             <  2:653732/150‑1 (MQ=255)
                                                                                                                                     tCCCGCCAGAAGGAGGGAGTTTTTTAGTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGAttt           <  1:161705/150‑1 (MQ=255)
                                                                                                                                       ccGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCGGTGATGGGACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCt         >  1:1262190/1‑150 (MQ=255)
                                                                                                                                         gCCAGAAGGCGGGAGTTTTTTACTGATTCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTg       <  1:793964/150‑1 (MQ=255)
                                                                                                                                          ccAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGc      <  1:1218744/150‑1 (MQ=255)
                                                                                                                                              aaGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCTAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAaca  <  1:1188728/150‑1 (MQ=255)
                                                                                                                                              aaGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAaca  <  2:1257687/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACA  >  NC_000913/662543‑662834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: