Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 672,147 A→T 6.1% L14I (TTA→ATA) ‡ lptE ← LPS assembly OM complex LptDE, lipoprotein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913672,1470AT 6.1% 315.6 / 7.1 112L14I (TTA→ATA) ‡lptELPS assembly OM complex LptDE, lipoprotein component
Reads supporting (aligned to +/- strand):  ref base A (42/61);  new base T (5/2);  total (49/63)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 118 total aligned reads displayed.

AAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGC  >  NC_000913/671998‑672295
                                                                                                                                                     |                                                                                                                                                    
aaGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGCTTaa                                                                                                                                                      >  1:1089031/1‑150 (MQ=255)
 aGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAac                                                                                                                                                     >  1:1101031/1‑150 (MQ=255)
         gACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCag                                                                                                                                             <  2:721542/150‑1 (MQ=255)
          acacCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCaga                                                                                                                                            <  1:264296/150‑1 (MQ=255)
          acacCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCaga                                                                                                                                            <  2:1049977/150‑1 (MQ=255)
            cccATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGTTCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGTAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAgaga                                                                                                                                          <  1:450980/149‑1 (MQ=255)
             ccATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGAt                                                                                                                                         >  1:59050/1‑150 (MQ=255)
                ttCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATaac                                                                                                                                      <  2:551060/150‑1 (MQ=255)
                   aGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATaacaac                                                                                                                                   <  1:206515/150‑1 (MQ=255)
                       gTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGACGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATg                                                                                                                               <  1:1701172/150‑1 (MQ=255)
                       gTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATg                                                                                                                               <  1:923519/150‑1 (MQ=255)
                          aCTGGTTACTCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGTATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTg                                                                                                                            <  1:259136/150‑1 (MQ=255)
                          aCTGGTTACGCACTGAACGGTTTAATGGCCCGTTCGGATCGCCTGAGTCCAGTATCATGACCTTCATAGTGGAAGGAACCTGCGTTGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTg                                                                                                                            <  1:1565395/150‑1 (MQ=255)
                            tGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGcc                                                                                                                          <  1:1557055/150‑1 (MQ=255)
                            tGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGcc                                                                                                                          >  1:1589105/1‑150 (MQ=255)
                             ggTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCa                                                                                                                         >  1:204243/1‑150 (MQ=255)
                              gTTACGCACCTCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAg                                                                                                                        <  2:1298637/150‑1 (MQ=255)
                              gTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAg                                                                                                                        <  1:1652422/150‑1 (MQ=255)
                                tACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGat                                                                                                                      >  1:286712/1‑150 (MQ=255)
                                  cgcaccgcacGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGatat                                                                                                                    <  2:730438/150‑1 (MQ=255)
                                  cgcaccgcacGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGGGGTATCACGCAGATGCCAGCCACACCCGGCGGGGCTGAACACCGCCAGAGATAACCACAATGTTGCCAGatat                                                                                                                    >  2:125050/1‑150 (MQ=255)
                                    caccgcacGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCg                                                                                                                  >  1:1383736/1‑150 (MQ=255)
                                        gcacGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGCCCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACg                                                                                                              >  1:1042144/1‑150 (MQ=255)
                                        gcacGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTACCACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACg                                                                                                              >  2:901290/1‑150 (MQ=255)
                                         cacGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCAAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGc                                                                                                             >  2:374358/1‑150 (MQ=255)
                                            ggCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTtc                                                                                                          <  1:181426/150‑1 (MQ=255)
                                            ggCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTtc                                                                                                          >  1:796612/1‑150 (MQ=255)
                                                   tGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCcgc                                                                                                   <  2:609660/150‑1 (MQ=255)
                                                    ggCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCcgcg                                                                                                  >  2:1435144/1‑150 (MQ=255)
                                                         gTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGACGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAg                                                                                             <  2:316329/150‑1 (MQ=255)
                                                           tCGGATCGCCTGAGTACAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCAGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGcc                                                                                           <  2:159033/150‑1 (MQ=255)
                                                             ggATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCaa                                                                                         >  1:424648/1‑150 (MQ=255)
                                                              gATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAAc                                                                                        >  2:945492/1‑150 (MQ=255)
                                                                tCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGTGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGa                                                                                      <  1:1230557/150‑1 (MQ=255)
                                                                tCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACAGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGa                                                                                      <  2:290501/150‑1 (MQ=255)
                                                                 cGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGAc                                                                                     <  2:40572/150‑1 (MQ=255)
                                                                   ccTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCa                                                                                   <  1:1552600/150‑1 (MQ=255)
                                                                    cTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAg                                                                                  >  2:1126478/1‑150 (MQ=255)
                                                                     tGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGa                                                                                 >  2:709388/1‑150 (MQ=255)
                                                                       aGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGAtt                                                                               <  2:213575/150‑1 (MQ=255)
                                                                        ttCCAGGCTCATGACCTTCATAGGTGAAGGAACCAGCGTGGTATCAGGGAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTg                                                                              <  2:1667925/149‑1 (MQ=255)
                                                                           cAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTgagg                                                                           <  1:367963/150‑1 (MQ=255)
                                                                             ggATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTgaggag                                                                         >  2:371154/1‑150 (MQ=255)
                                                                              gATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGt                                                                        <  2:204243/150‑1 (MQ=255)
                                                                                  aTGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTAc                                                                    >  1:927893/1‑150 (MQ=255)
                                                                                        ttCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTa                                                              <  1:1354043/150‑1 (MQ=255)
                                                                                         tCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTAc                                                             <  2:491819/150‑1 (MQ=255)
                                                                                         tCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTAc                                                             <  2:491816/150‑1 (MQ=255)
                                                                                             aGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTaa                                                         >  2:1496911/1‑150 (MQ=255)
                                                                                                ggaaggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATc                                                      <  2:1008482/150‑1 (MQ=255)
                                                                                                ggaaggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACAACGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATc                                                      >  2:1058083/1‑150 (MQ=255)
                                                                                                cgaaggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATc                                                      >  1:706469/2‑150 (MQ=255)
                                                                                                  aaggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCAc                                                    <  2:772706/150‑1 (MQ=255)
                                                                                                   aggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACt                                                   <  2:1589105/150‑1 (MQ=255)
                                                                                                   aggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACt                                                   <  2:1518553/150‑1 (MQ=255)
                                                                                                    ggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGTCAACGACCAGATTGAGGAGTTTACCTGGTACGTaaagtgatt                                                  <  2:1338419/150‑7 (MQ=255)
                                                                                                    ggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACtt                                                  <  1:475768/150‑1 (MQ=255)
                                                                                                    ggaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACtt                                                  <  2:870399/150‑1 (MQ=255)
                                                                                                     gaaCCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACttt                                                 <  2:463691/150‑1 (MQ=255)
                                                                                                        ccTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCACCGACCAGATTGAGGAGTTTACCTGGTACGTAAATAACTTTaag                                              >  2:975311/1‑148 (MQ=255)
                                                                                                         cTGCGTGATACCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGt                                             >  1:363265/1‑150 (MQ=255)
                                                                                                          tGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTa                                            >  1:1057642/1‑150 (MQ=255)
                                                                                                            gggggTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAAGTGATTTACGTAcc                                          <  2:885739/147‑2 (MQ=255)
                                                                                                            cGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGCCCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACa                                          >  2:42455/1‑150 (MQ=255)
                                                                                                            cGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACa                                          <  2:59050/150‑1 (MQ=255)
                                                                                                            cGTGGTATCACGCAGATGCCAGCCACACCCGGCGGGGATTAACACCGCGAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTTCGTAAATCACTTTCCGTACa                                          >  1:140054/1‑150 (MQ=255)
                                                                                                              tGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGt                                        <  1:712820/150‑1 (MQ=255)
                                                                                                                 tATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTatc                                     >  2:235774/1‑148 (MQ=255)
                                                                                                                  aTCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACg                                    <  1:952442/150‑1 (MQ=255)
                                                                                                                    cACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGcc                                  >  1:65441/1‑150 (MQ=255)
                                                                                                                    cACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGcc                                  >  1:514374/1‑150 (MQ=255)
                                                                                                                    cACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGcc                                  <  2:796612/150‑1 (MQ=255)
                                                                                                                     aCGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCa                                 >  2:1103951/1‑150 (MQ=255)
                                                                                                                      cGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCAt                                >  2:1108764/1‑150 (MQ=255)
                                                                                                                          gATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAg                            <  1:348681/150‑1 (MQ=255)
                                                                                                                               cagccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATAtt                       >  2:532504/1‑150 (MQ=255)
                                                                                                                                 gccaCACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATAtttt                     <  2:424648/150‑1 (MQ=255)
                                                                                                                                     cacCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGa                           <  1:685963/140‑1 (MQ=255)
                                                                                                                                     cacCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGa                           >  2:685963/1‑140 (MQ=255)
                                                                                                                                     cacCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTa                 >  2:46565/1‑150 (MQ=255)
                                                                                                                                      ctctggcggTGTTAATCACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTAc                >  1:567503/5‑150 (MQ=255)
                                                                                                                                       tctggcggTGTTAATCACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTAcc               >  1:368924/4‑150 (MQ=255)
                                                                                                                                       cccGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTAcc               <  2:1101031/150‑1 (MQ=255)
                                                                                                                                       cccGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTAcc               >  1:3395/1‑150 (MQ=255)
                                                                                                                                       cccGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCAGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTAcc               <  2:1042144/150‑1 (MQ=255)
                                                                                                                                        ctggcggTGTTAATCACCGCCGGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCa              >  1:1141/3‑150 (MQ=255)
                                                                                                                                        ctggcggTGTTAATCACCGCCAGGGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGa                           <  1:129898/135‑1 (MQ=255)
                                                                                                                                        ctggcggTGTTAATCACCGCCAGGGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGa                           >  2:129898/3‑137 (MQ=255)
                                                                                                                                        ctggcggTGTTAATCACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCa              >  1:1587069/3‑150 (MQ=255)
                                                                                                                                         cggcggTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAg             <  1:1692947/150‑1 (MQ=255)
                                                                                                                                         cggcggTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAg             <  1:709388/150‑1 (MQ=255)
                                                                                                                                           gcggTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGAt           >  1:559696/1‑150 (MQ=255)
                                                                                                                                              gTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGtt        <  1:1244460/150‑1 (MQ=255)
                                                                                                                                              gTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACAAGATGtt        >  2:1387718/1‑150 (MQ=255)
                                                                                                                                               tgcttAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACCACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGttg       >  1:326930/4‑149 (MQ=255)
                                                                                                                                                  ttACCACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTg    >  1:867586/1‑150 (MQ=255)
                                                                                                                                                   tAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTgg   <  2:706469/150‑1 (MQ=255)
                                                                                                                                                   tAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTgg   <  2:927893/150‑1 (MQ=255)
                                                                                                                                                    aaCACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTTCCTGGTACGTATATCCCTTTACGTTCAGTAACGCCATCAAGATATTTTGCGACCAGATGTTCCTGGc  >  2:772707/1‑150 (MQ=255)
                                                                                                                                                    aaCACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGc  >  1:1152336/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAGTGGAAGGAACCTGCGTGGTATCACGCAGATGCCAGCCACACCCGGCGGTGATTAACACCGCCAGAGATAACAACAATGTTGCCAGATATCGCACGCTTCCTCCCGCGCTTAGCCAACGACCAGATTGAGGAGTTTACCTGGTACGTAAATCACTTTACGTACAGTAACGCCATCAAGATATTTTGCTACCAGATGTTCCTGGC  >  NC_000913/671998‑672295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: