Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 681,699 T→A 8.4% intergenic (+36/+24) djlC → / ← hscC J domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913681,6990TA 8.4% 262.7 / 11.9 95intergenic (+36/+24)djlC/hscCJ domain‑containing HscC co‑chaperone; Hsc56/Hsp70 family chaperone Hsc62; RpoD‑binding transcription inhibitor
Reads supporting (aligned to +/- strand):  ref base T (44/43);  new base A (4/4);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01

AAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTCTCGCTCGTC  >  NC_000913/681551‑681848
                                                                                                                                                    |                                                                                                                                                     
aaaaaaaTGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGttt                                                                                                                                                      >  2:1304204/1‑150 (MQ=255)
          cgaGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGAAAGGGTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTATTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATc                                                                                                                                            <  1:1586225/149‑1 (MQ=255)
           gaGGGTGACAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATcc                                                                                                                                           <  2:475665/150‑1 (MQ=255)
            ccggTGAAAAACCACAGAGCATTACCAGCATAATCAAACTCAGTTTAACGGTGCTCGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCg                                                                                                                                          <  1:681685/148‑1 (MQ=255)
             gggTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCgg                                                                                                                                         <  2:983543/150‑1 (MQ=255)
             gggTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                                                                         <  1:793356/150‑1 (MQ=255)
             gggTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                                                                         >  2:707778/1‑150 (MQ=255)
             gggTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                                                                         >  2:339548/1‑150 (MQ=255)
             gggTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCgg                                                                                                                                         >  1:1698763/1‑150 (MQ=255)
              ggTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGc                                                                                                                                        <  1:1593271/150‑1 (MQ=255)
                  aaaaaCCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGcc                                                                                                                                    >  1:540349/1‑150 (MQ=255)
                   aaaaCCACAGAGCAATACCACCATAATCAAAATCAGTTTAAGGGTGCTGGTGATAGGATATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCGGCGTAAACGCTGCATCCGGCatcgg                                                                                                                                   <  2:1103609/150‑5 (MQ=38)
                    aaaCCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCgt                                                                                                                                  >  2:1723961/1‑150 (MQ=255)
                     aaCCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCgtg                                                                                                                                 <  2:1132991/150‑1 (MQ=255)
                     aaCCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCgtg                                                                                                                                 <  2:1486223/150‑1 (MQ=255)
                     aaCCACAGAGCATAACCAGCATTATCAAAATCTGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAACTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCGGGCAGCCgtg                                                                                                                                 <  2:495710/150‑1 (MQ=255)
                     aaCCACACTGCATTACCAGCATTATCAAAATCAGTTTAACGGAGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCgtg                                                                                                                                 <  2:844162/150‑1 (MQ=255)
                      aCCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGCGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCgtgt                                                                                                                                <  1:143630/150‑1 (MQ=255)
                       caacaTAGCACTACCAGCAATAGCAAAAACAGTTTAACGCTGCTGGTGATAGGTTATGCTCTCGTCAAAATCGCGATGTTGGTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCACCCGGCAGCCGTGtt                                                                                                                               <  1:852369/148‑1 (MQ=255)
                         acaGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTa                                                                                                                             >  2:493790/1‑150 (MQ=255)
                            gagCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCTCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCAGTGTTTATgg                                                                                                                          >  2:874428/1‑150 (MQ=255)
                            gagCAATACCAGCATTATCAAAATCAGTTTAACGGTGCGGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCAGCAGCCGTGTTTATgg                                                                                                                          <  2:1246797/150‑1 (MQ=255)
                             cgCATTACCAGCATTATCAAAATACGTTTAACGGTGCTGGTGATAGGTAATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATggg                                                                                                                         <  2:1702304/149‑1 (MQ=255)
                                 ttACCAGCATTATCAAAATCATTTTAACGGTGCTGGTGATAGGTTATGCTCTCGTCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtct                                                                                                                     <  2:1630570/150‑1 (MQ=255)
                                  tACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGAtctc                                                                                                                    >  2:462374/1‑150 (MQ=255)
                                   aCCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCa                                                                                                                   <  2:1526356/150‑1 (MQ=255)
                                     cAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAAt                                                                                                                 >  2:258811/1‑150 (MQ=255)
                                      aGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                                                                                <  1:976996/150‑1 (MQ=255)
                                      aGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                                                                                <  2:168470/150‑1 (MQ=255)
                                      aGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                                                                                <  2:645443/150‑1 (MQ=255)
                                      aGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTAATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATg                                                                                                                <  1:1126250/150‑1 (MQ=255)
                                          ctATCAAACTAAGATTAACGGTGCGGCGGATAGGTTATTCTCTCGGCAAAATCGCGATGAGGTTTAGATGACGATGTGAGTCTTGCGCCGATGCCGGACGCAGCGTTTACGCCTCATCCGGCAGCCGTGTTTATCCGAGCTCAATGGCTa                                                                                                            <  2:100635/149‑1 (MQ=255)
                                           tATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTaa                                                                                                           >  2:750531/1‑150 (MQ=255)
                                           tATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTaa                                                                                                           >  2:744158/1‑150 (MQ=255)
                                            aTCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTaaa                                                                                                          >  1:1121848/1‑150 (MQ=255)
                                             acAAAATAAGTTTAACGCTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGAAGTTGTTAAGCTTAACATCTGTTTTTTGCGCCGATGCCGGCTTCAGCGTTTACTCAGCATCCGTCTGACGTGATAATGGCATCTCAAAGCCAAaat                                                                                                         >  1:1520696/2‑150 (MQ=255)
                                              cAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAaata                                                                                                        >  1:1704654/1‑150 (MQ=255)
                                                  aTCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcg                                                                                                    <  2:407001/150‑1 (MQ=255)
                                                     aGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTAAGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctc                                                                                                 >  1:540032/1‑150 (MQ=255)
                                                      gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAAtcgctcg                                                                                                >  1:1470002/1‑150 (MQ=255)
                                                      gTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTAGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCCGCCGTGTTTATGGGATCGCAAAGGCTAAAAAAtcgctcg                                                                                                >  1:850328/1‑150 (MQ=255)
                                                          aaCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccc                                                                                            >  2:1576816/1‑149 (MQ=255)
                                                           aCGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTcgcccgc                                                                                           >  1:110414/1‑150 (MQ=255)
                                                                    gTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTATATAATCGCTCGCCCGCCGCCGAACt                                                                                  >  1:1642118/1‑150 (MQ=255)
                                                                     tGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGAAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACtt                                                                                 >  2:1246655/1‑150 (MQ=255)
                                                                      gATAGGTTATGCTCTCGGCTAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTc                                                                                <  1:1730552/150‑1 (MQ=255)
                                                                      gATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGAGTTTACGCCGCATCCGGCAGCAGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCAGCCGCCGCACtta                                                                                >  1:1440464/1‑149 (MQ=255)
                                                                       aTAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCa                                                                               <  2:471744/150‑1 (MQ=255)
                                                                       aTAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCa                                                                               <  2:427813/150‑1 (MQ=255)
                                                                         aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                                                                             >  2:1624835/1‑150 (MQ=255)
                                                                         aGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                                                                             <  1:808768/150‑1 (MQ=255)
                                                                          ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATcc                                                                            <  2:110414/150‑1 (MQ=255)
                                                                          ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATcc                                                                            >  1:572350/1‑150 (MQ=255)
                                                                          ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATcc                                                                            >  1:753263/1‑150 (MQ=255)
                                                                          ggTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATAGCTCGCCCGCCGCCGAAGTTCATcc                                                                            >  2:136839/1‑150 (MQ=255)
                                                                             tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                                                                             <  2:1180312/146‑1 (MQ=255)
                                                                             tATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                                                                             >  1:1180312/1‑146 (MQ=255)
                                                                               tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCAcgcg                                                                       >  2:13190/1‑150 (MQ=255)
                                                                                gCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGTTCTCAATGGCTAAATAATCGCTCGCCCGCCCCCGCACTTCATCCACGCgg                                                                      >  2:1039710/1‑150 (MQ=255)
                                                                                  tctcGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGc                                                                    <  1:258811/150‑1 (MQ=255)
                                                                                     cGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCat                                                                 >  2:1652091/1‑150 (MQ=255)
                                                                                        cAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATc                                                              >  1:1548247/1‑150 (MQ=255)
                                                                                           aaTCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGtt                                                           <  2:469081/150‑1 (MQ=255)
                                                                                             tCGCGTTGTTGTTTAGCTGACGATGTGTTTTTTGCTCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGGAGCCTTGTTTATGGGTTCTCAATGGCTAAATAATGGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg                                                         <  1:120199/150‑1 (MQ=255)
                                                                                             tCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg                                                         <  1:959651/150‑1 (MQ=255)
                                                                                             tCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg                                                         <  1:480234/150‑1 (MQ=255)
                                                                                             tCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTg                                                         <  2:208061/150‑1 (MQ=255)
                                                                                              cgcaATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGa                                                        >  2:1649696/1‑150 (MQ=255)
                                                                                               gcgATGTTGTTTAGCTGACGCTGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGAc                                                       <  1:837374/150‑1 (MQ=255)
                                                                                                      tgttTAGCTGCGGATGTGTTTTTTGCGCCGCTGCCGTATGCTGCGTTTACGCGGCATCCGGCAGCCGTGTTTATGGTATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAAcg                                                <  2:1520833/150‑1 (MQ=255)
                                                                                                          tAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCCTCCGGCCGCCGGGTTTATGGGATCGCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCCCGCGCGCCATATCGTTTGCCGGCAAcgccgc                                            >  2:58312/1‑150 (MQ=255)
                                                                                                           aGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCa                                           >  2:1197154/1‑150 (MQ=255)
                                                                                                             cTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATc                                         >  2:1580176/1‑150 (MQ=255)
                                                                                                               gACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCaa                                       >  2:704897/1‑150 (MQ=255)
                                                                                                               gACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCaa                                       >  2:531054/1‑150 (MQ=255)
                                                                                                               gACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTAATGGGATCACAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCCCGCGGGCCATATCGTTTGACTGCAACGCCGCATCaa                                       >  2:1539901/1‑150 (MQ=255)
                                                                                                                   aTGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATc                                   <  1:13190/150‑1 (MQ=255)
                                                                                                                       gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTg                               <  2:1631565/150‑1 (MQ=255)
                                                                                                                       gtTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGACGCCGAACTACATCCCCGCGGGCCATATCGTTTGACTGCACCGGCCCATCAAAATCGGTg                               >  2:323841/1‑150 (MQ=255)
                                                                                                                        ttttttGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGa                              <  2:1698763/150‑1 (MQ=255)
                                                                                                                                  ggctGCCGGATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGcc                    >  1:1058402/4‑150 (MQ=255)
                                                                                                                                   gATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGCCTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCg                   >  2:1282540/1‑150 (MQ=255)
                                                                                                                                    aTGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCgc                  >  1:542993/1‑149 (MQ=255)
                                                                                                                                      gCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGAtc                <  2:618870/150‑1 (MQ=255)
                                                                                                                                       ccGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGAtct               >  1:1720001/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGAtct               <  1:1723961/150‑1 (MQ=255)
                                                                                                                                         ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATctct             >  2:523584/1‑150 (MQ=255)
                                                                                                                                         ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATctct             >  2:3263/1‑150 (MQ=255)
                                                                                                                                         ggATGCGGCGTAAACGCTGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATctct             >  1:698530/1‑150 (MQ=255)
                                                                                                                                         ggATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATctct             <  2:572350/150‑1 (MQ=255)
                                                                                                                                             gCAGCGTTTACGCCGCATCCGTCAGCCGGGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCCCGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTtctc         <  2:237777/150‑1 (MQ=255)
                                                                                                                                              cAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTctcg        >  1:402311/1‑150 (MQ=255)
                                                                                                                                              cAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTctcg        >  2:183072/1‑150 (MQ=255)
                                                                                                                                                gCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTctcgct      <  1:1651710/150‑1 (MQ=255)
                                                                                                                                                gCGTTTACGCCGCAGCGGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTctcgct      <  1:1334022/150‑1 (MQ=255)
                                                                                                                                                  gTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTctcgctcg    <  2:1345705/150‑1 (MQ=255)
                                                                                                                                                    ttACGCCGCATCCGGCAGCCGGGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTGCCTCCCCGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGGGAACACCCGGCCAAGCTCTTCTCGCTCGTc  >  2:881777/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTCTCGCTCGTC  >  NC_000913/681551‑681848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: