Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 693,030 C→A 5.9% V117L (GTA→TTA)  ybeZ ← heat shock protein, putative NTPase; PhoH‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913693,0300CA 5.9% 285.5 / 5.8 93V117L (GTA→TTA) ybeZheat shock protein, putative NTPase; PhoH‑like protein
Reads supporting (aligned to +/- strand):  ref base C (41/46);  new base A (2/4);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACA  >  NC_000913/692881‑693178
                                                                                                                                                     |                                                                                                                                                    
gCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAc                                                                                                                                                      >  1:896353/1‑150 (MQ=255)
gCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAAAGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGGCTGTGTTGGCGTGCGCGGCTTAATTAc                                                                                                                                                      >  1:698188/1‑150 (MQ=255)
      ttCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCcgcg                                                                                                                                                <  2:623098/150‑1 (MQ=255)
        ccTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGtt                                                                                                                                              >  2:1252144/1‑150 (MQ=255)
        ccTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGtt                                                                                                                                              >  2:1265627/1‑150 (MQ=255)
         cTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcac                                                                                                                                             <  1:318622/150‑4 (MQ=255)
          tGACGCTCCAGGGCATCAACTGCCGCAGCGACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTg                                                                                                                                            >  2:1538618/1‑150 (MQ=255)
          tGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTg                                                                                                                                            <  2:113594/150‑1 (MQ=255)
          tGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcacg                                                                                                                                            <  1:1671588/150‑5 (MQ=255)
          tGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcacg                                                                                                                                            <  1:749896/150‑5 (MQ=255)
          tGACGCTCCACGGCATCAACTGCCGCACCCACTGCCAGGTAGCTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCAAGGTCGAGAATATAGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcacg                                                                                                                                            <  1:36094/150‑5 (MQ=255)
           gACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGGTTgg                                                                                                                                           >  2:890326/1‑150 (MQ=255)
                tCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTg                                                                                                                                      <  2:1664307/150‑1 (MQ=255)
                 ccAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGGTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGa                                                                                                                                     <  1:1075752/150‑1 (MQ=255)
                  cAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGat                                                                                                                                    >  1:1136457/1‑150 (MQ=255)
                  cAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGGTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGat                                                                                                                                    <  2:557667/150‑1 (MQ=255)
                      gCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTg                                                                                                                                <  2:653419/150‑1 (MQ=255)
                      gCATCAACAGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTg                                                                                                                                <  1:1285354/150‑1 (MQ=255)
                        aTCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGAc                                                                                                                              >  1:1191172/1‑150 (MQ=255)
                         tCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGGTTTGATATTGAcc                                                                                                                             <  2:983980/150‑1 (MQ=255)
                         tCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACACGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGAcc                                                                                                                             <  1:499914/150‑1 (MQ=255)
                          cAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCg                                                                                                                            >  2:1439598/1‑150 (MQ=255)
                               gCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTg                                                                                                                       >  1:284295/1‑150 (MQ=255)
                                ccGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGc                                                                                                                      <  2:1366115/150‑1 (MQ=255)
                                 cGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGcc                                                                                                                     >  2:1161321/1‑150 (MQ=255)
                                  gCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCg                                                                                                                    >  2:787966/1‑150 (MQ=255)
                                   cAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGCCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGt                                                                                                                   <  1:1607315/150‑1 (MQ=255)
                                    aGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTa                                                                                                                  >  1:899855/1‑150 (MQ=255)
                                     gCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAAGCCGCGTTTGGTTTTGATATTTACCGCTTATCCGTAc                                                                                                                 >  2:1275580/1‑150 (MQ=255)
                                        aCTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTcc                                                                                                              <  1:460478/150‑1 (MQ=255)
                                        aCTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCATAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTcc                                                                                                              >  2:1592932/1‑150 (MQ=255)
                                                 taggtTTTACCCGTACCCGCCGGGCCATCGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGctc                                                                                                     >  1:1707480/1‑150 (MQ=255)
                                                  aggtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcgccgc                                                                                                                                                 <  2:909064/105‑1 (MQ=255)
                                                  aggtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcgccgc                                                                                                                                                 >  1:909064/1‑105 (MQ=255)
                                                    gtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTcc                                                                                                  <  1:134014/150‑1 (MQ=255)
                                                     ttttACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGctcgacg                                                                                                 >  1:913370/1‑146 (MQ=255)
                                                        tacccgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCgcgc                                                                                              <  2:23590/150‑1 (MQ=255)
                                                          cccgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCtt                                                                                            >  2:121742/1‑150 (MQ=255)
                                                          cccgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCtt                                                                                            >  1:140628/1‑150 (MQ=255)
                                                           ccgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCttt                                                                                           <  2:233156/150‑1 (MQ=255)
                                                             gtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGc                                                                                         <  2:540694/150‑1 (MQ=255)
                                                              tagccgccgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCTTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCt                                                                                        <  2:1563047/147‑1 (MQ=255)
                                                              tacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCt                                                                                        <  2:1605875/150‑1 (MQ=255)
                                                              tacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCt                                                                                        <  1:695095/150‑1 (MQ=255)
                                                              tacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCt                                                                                        <  1:1538618/150‑1 (MQ=255)
                                                                cccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTcc                                                                                      <  2:1136457/150‑1 (MQ=255)
                                                                cccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTcc                                                                                      <  1:1265627/150‑1 (MQ=255)
                                                                  cgccgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCCCGTTTGGTTTTGATATTGACCGCTTTGCCGTTCTCCGGCACGCTCTCCGCGCTTTGCTCCAg                                                                                    >  2:1078831/1‑150 (MQ=255)
                                                                    ccgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTa                                                                                  >  2:243581/1‑150 (MQ=255)
                                                                      tggCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGGTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGTCACGCTCTCCGCGCTTTGCTCCCGTaac                                                                                >  1:84893/2‑148 (MQ=255)
                                                                              gCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGcttc                                                                        >  2:623272/1‑150 (MQ=255)
                                                                              gCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGcttc                                                                        >  1:494394/1‑150 (MQ=255)
                                                                                cGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGcttctt                                                                      >  1:214261/1‑150 (MQ=255)
                                                                                cGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGcttctt                                                                      >  2:413879/1‑150 (MQ=255)
                                                                                cGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGcttctt                                                                      <  2:714073/150‑1 (MQ=255)
                                                                                cGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGcttctt                                                                      <  1:1248718/150‑1 (MQ=255)
                                                                                cGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGcttctt                                                                      <  2:777163/150‑1 (MQ=255)
                                                                                 gAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCttt                                                                     <  2:378926/150‑1 (MQ=255)
                                                                                 gAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCttt                                                                     <  2:521650/150‑1 (MQ=255)
                                                                                    ggTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAAt                                                                  <  2:548794/150‑1 (MQ=255)
                                                                                    ggTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAAt                                                                  >  1:390341/1‑150 (MQ=255)
                                                                                        aTGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCCCGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCa                                                              >  2:330415/1‑150 (MQ=255)
                                                                                        aTGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCa                                                              <  2:1417577/150‑1 (MQ=255)
                                                                                          gTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAg                                                            >  1:5700/1‑150 (MQ=255)
                                                                                              tGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTATGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTgg                                                        >  1:174568/1‑150 (MQ=255)
                                                                                              tGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGCTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTgg                                                        >  2:471392/1‑150 (MQ=255)
                                                                                               ggTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGa                                                       >  1:697020/1‑150 (MQ=255)
                                                                                                 tCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATc                                                     >  1:442130/1‑150 (MQ=255)
                                                                                                  cGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCt                                                    <  1:623564/150‑1 (MQ=255)
                                                                                                   gagaATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTg                                                   >  2:32662/1‑150 (MQ=255)
                                                                                                     taaTATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCTCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGtt                                                 <  1:1225979/149‑1 (MQ=255)
                                                                                                      tatatTGGCGATGTACTGCGCCTGGTTTGTCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTc                                                <  1:164361/149‑1 (MQ=255)
                                                                                                         atTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCgg                                             >  1:635715/1‑150 (MQ=255)
                                                                                                            ggCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTc                                          <  1:1355906/150‑1 (MQ=255)
                                                                                                            ggCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTc                                          <  1:485595/150‑1 (MQ=255)
                                                                                                             gcgcTGTACTGCGCCTGGGTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCg                                         <  1:1436519/150‑1 (MQ=255)
                                                                                                             gCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCTCTTTGCCGTACTCCGTCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGTATCTGTTCCGGTTCg                                         <  1:1275580/150‑1 (MQ=255)
                                                                                                             gCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCg                                         <  2:1247745/150‑1 (MQ=255)
                                                                                                              cGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTTATCGCAAGGTGGATCTGTTCCGGTTCGa                                        >  1:851279/1‑150 (MQ=255)
                                                                                                               gATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGat                                       <  2:411511/150‑1 (MQ=255)
                                                                                                                   tACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATcc                                   >  1:935511/1‑150 (MQ=255)
                                                                                                                   tACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCGTTAATCGCAAGGGGGATCTGTTCCGGTTCGATATcc                                   >  1:1707757/1‑150 (MQ=255)
                                                                                                                     cTGCGCCTGGTTTGGCGTGCGCGGGTTAATTACGCCGCGGTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTGCGTTAATCGCAAGGTGGATCTGTTCCGGTGCGATATCCTg                                 >  1:1256543/1‑150 (MQ=255)
                                                                                                                        cgcCTGGTTCGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACAGCATTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGGGGATCTGTTCCGGTTCGATATCCTGAAt                              <  2:132828/150‑1 (MQ=255)
                                                                                                                        cgcCGGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAAt                              <  1:1180899/150‑1 (MQ=255)
                                                                                                                         gcCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATc                             <  1:1252144/150‑1 (MQ=255)
                                                                                                                            tGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGGGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGa                          >  2:326001/1‑150 (MQ=255)
                                                                                                                               tttGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACcg                       <  1:787966/150‑1 (MQ=255)
                                                                                                                                  accaaacgcgGCGTAATTAAGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCgcgc                    >  1:1180555/7‑150 (MQ=255)
                                                                                                                                    cGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTTCTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCAt                  >  1:24383/1‑150 (MQ=255)
                                                                                                                                    cGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCAt                  >  2:1322877/1‑150 (MQ=255)
                                                                                                                                     gTGCGCGGCTTAATTACGCCGCGTTTGGGTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATc                 <  1:1071703/150‑1 (MQ=255)
                                                                                                                                             cTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCAGCGCTTTGCTCCAGTACACTCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGt         >  1:190023/1‑150 (MQ=255)
                                                                                                                                              ttAATTACGCCGCGTTTGGCTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTa        <  1:471392/150‑1 (MQ=255)
                                                                                                                                                aaTTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATc      >  2:1665866/1‑150 (MQ=255)
                                                                                                                                                 aTTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCg     <  2:284295/150‑1 (MQ=255)
                                                                                                                                                    aCGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACa  <  2:899855/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACA  >  NC_000913/692881‑693178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: