Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 699,439 G→T 5.4% intergenic (‑262/+135) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913699,4390GT 5.4% 329.5 / 6.8 112intergenic (‑262/+135)asnB/umpHasparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base G (50/56);  new base T (3/3);  total (53/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-01

Only 100 of 116 total aligned reads displayed.

ATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGAT  >  NC_000913/699296‑699588
                                                                                                                                               |                                                                                                                                                     
aTTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGACGGATGCGGCGTGAACGcc                                                                                                                                                 >  1:866135/1‑150 (MQ=255)
  tGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCtt                                                                                                                                               >  2:868823/1‑150 (MQ=255)
   gTCGATTGGTGTAAAAAGGTCTGGGGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTTCGGGGGCCAAATTGCTGACAAAGTGCGAGTTGTTCATGCCGGATGCGGCGGGAACGCCTTa                                                                                                                                              <  1:1178953/150‑1 (MQ=39)
     cGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                            >  2:1695272/1‑150 (MQ=255)
      gaatGGTGAAAAAAGGGCTGGGGTGAGGATATATTTATTTATGAATCTATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGAGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                           <  2:456925/147‑1 (MQ=38)
        ttGGTGAAAAAAGGTCTTGTGTGAGGATATATTTATTGATGAATCGATAATTATTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                         <  1:862211/150‑1 (MQ=255)
         tGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                                                                                                        <  2:931899/150‑1 (MQ=255)
          ggTGAAAACAGGTCTGGTGTGAGGAAATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                       <  1:921358/150‑1 (MQ=255)
          ggTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTTCGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                       <  1:1221098/150‑1 (MQ=255)
          ggTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                                       <  1:628279/150‑1 (MQ=255)
           gTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                                                                                                      >  2:184202/1‑150 (MQ=255)
           gTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                                                                                                      <  2:61875/150‑1 (MQ=255)
           gTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGcat                                                                                                                                      <  2:1573776/150‑3 (MQ=39)
            tGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGTGGGCCAAATTGATGACAAATTTAGATTTTTTCATTCAGGATTCTGCGTGAACTCCTTATCCGACCTa                                                                                                                                     >  1:1077524/1‑150 (MQ=255)
            tGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa                                                                                                                                     >  2:592923/1‑150 (MQ=255)
              aaaaaaGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa                                                                                                                                   <  2:1456951/150‑1 (MQ=255)
               aaaaaGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaa                                                                                                                                  <  1:1670063/150‑1 (MQ=255)
                aaaaGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATAAGGCGTTCACGCCGCATCCGGcatgaaca                                                                                                                                 <  1:499259/150‑8 (MQ=38)
                 aaaGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaaa                                                                                                                                <  2:1141537/150‑1 (MQ=255)
                 aaaGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaaaa                                                                                                                                <  2:1239286/150‑1 (MQ=255)
                    ggTCTGGTGTGAGGATATAATTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATTCGGCGTGAACGCCTTATCCGGCCTACAAAAGCa                                                                                                                             >  2:1013730/1‑150 (MQ=255)
                     gTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCAt                                                                                                                            >  2:984540/1‑150 (MQ=255)
                        tGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGaa                                                                                                                         >  1:1129472/1‑150 (MQ=255)
                          gtgtGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGaaaa                                                                                                                       <  1:611017/150‑1 (MQ=255)
                           tgtgAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCTAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAAt                                                                                                                      >  2:738732/1‑150 (MQ=255)
                            gtgAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAAtt                                                                                                                     >  1:1589818/1‑150 (MQ=255)
                             tgAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGATTGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATATGTTCATGCCGGATCCGGCGTGAACGCCTAATCAGGCCTACAAAAGCATGAAAAtta                                                                                                                    >  2:347566/1‑149 (MQ=255)
                             tgAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAAtta                                                                                                                    >  2:1728669/1‑149 (MQ=255)
                              gAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCa                                                                                                                   >  2:405063/1‑150 (MQ=255)
                                ggATATATTTATTGATGAATCGATAATTTTTAGCGGGGTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAat                                                                                                                 <  1:174809/150‑1 (MQ=255)
                                 gATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGACTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAata                                                                                                                >  2:672338/1‑150 (MQ=255)
                                     tatttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATAtt                                                                                                            >  2:929861/1‑150 (MQ=255)
                                      atttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTg                                                                                                           <  1:765201/150‑1 (MQ=255)
                                      atttattGATGAATCCATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTg                                                                                                           >  1:427206/1‑150 (MQ=255)
                                       tttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGc                                                                                                          <  2:873422/150‑1 (MQ=255)
                                       tttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGc                                                                                                          >  2:56909/1‑150 (MQ=255)
                                        ttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCa                                                                                                         >  2:751937/1‑150 (MQ=255)
                                         tattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTTCTGACAAAGTTAGATTTGTTAATGAAGGATGCGTCGTGAACTCATTATCCGGCCTACAAAAGAATGAAAATTAAAGATATTGCAg                                                                                                        >  1:245789/1‑150 (MQ=255)
                                         tattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAg                                                                                                        >  1:743572/1‑150 (MQ=255)
                                            tgatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAg                                                                                                     <  2:195683/150‑1 (MQ=255)
                                            tgatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAg                                                                                                     >  2:1445830/1‑150 (MQ=255)
                                             gatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATACTGCAGGAGc                                                                                                    >  2:270986/1‑150 (MQ=255)
                                             gaagaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGAGCGATTTGTTCATGCCTGAAGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGc                                                                                                    <  2:1266419/147‑1 (MQ=255)
                                               tgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTg                                                                                                  >  2:1360463/1‑150 (MQ=255)
                                               tgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTg                                                                                                  >  2:836230/1‑150 (MQ=255)
                                                gaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGc                                                                                                 >  1:20583/1‑150 (MQ=255)
                                                gaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCACGCCGCATCCGGCATg                                                                                                                                                       <  1:60751/96‑1 (MQ=255)
                                                     gATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAgg                                                                                            >  2:884941/1‑150 (MQ=255)
                                                     gATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAgg                                                                                            >  2:450204/1‑150 (MQ=255)
                                                       tAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGcc                                                                                          <  1:555685/150‑1 (MQ=255)
                                                        aaTTTTTAGCTGGTTTTATTGAATGTTTATATTTTTCGTGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGTATGCGTCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCt                                                                                         <  2:306494/150‑1 (MQ=37)
                                                        aaTTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCt                                                                                         <  2:1624213/150‑1 (MQ=255)
                                                        aaTTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCt                                                                                         <  2:385278/150‑1 (MQ=255)
                                                         aTTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTg                                                                                        <  2:944606/150‑1 (MQ=255)
                                                         aTTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTg                                                                                        <  2:452094/150‑1 (MQ=255)
                                                           ttttAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGAt                                                                                      <  2:172360/150‑1 (MQ=255)
                                                              tAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAg                                                                                   <  2:1261910/150‑1 (MQ=255)
                                                              tAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAg                                                                                   >  1:871878/1‑150 (MQ=255)
                                                               aGCGTGTTTTATTGAATGTTTATATTATCCTGGGGACAAATTTCTGACAATCTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATCAAAATTCAATATATTGCAGGAGCTGCGTAGGCCGGATAAGc                                                                                  <  1:846646/150‑1 (MQ=21)
                                                               aGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGATCTGCGTAGGCCTGATAAGc                                                                                  >  2:98877/1‑150 (MQ=255)
                                                                gagggTTTTATTGAATGTTTATATTTTACGGGGGCCATATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCg                                                                                 <  1:1642629/148‑1 (MQ=255)
                                                                gCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCg                                                                                 <  1:1411774/150‑1 (MQ=255)
                                                                   ggTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCTTGAACGCCTTATCCGGCCTACAAAAGCATGATAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAg                                                                              >  1:56420/1‑150 (MQ=255)
                                                                   ggTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAg                                                                              <  1:1136563/150‑1 (MQ=255)
                                                                     ttttATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAgcg                                                                            >  1:917565/1‑150 (MQ=255)
                                                                       ttATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCa                                                                          <  1:1529657/150‑1 (MQ=255)
                                                                         aTTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATc                                                                        >  1:944749/1‑150 (MQ=255)
                                                                          ttGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCa                                                                       <  2:652164/150‑1 (MQ=255)
                                                                           tGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAg                                                                      <  2:1190788/150‑1 (MQ=255)
                                                                             aaTGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                    <  2:1270321/150‑1 (MQ=255)
                                                                                     tatTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAATTTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGc                                                            <  1:381522/150‑1 (MQ=255)
                                                                                     tatTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGc                                                            <  1:1487334/150‑1 (MQ=255)
                                                                                      atTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGCGCTGCGTCGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCg                                                           >  1:1095116/1‑150 (MQ=255)
                                                                                                ggCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTTAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGtcatca                                                 <  1:26452/150‑1 (MQ=255)
                                                                                                  ccAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCaga                                               >  1:540233/1‑150 (MQ=255)
                                                                                                   cAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCagag                                              >  2:1265614/1‑150 (MQ=255)
                                                                                                   cAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCagag                                              <  2:20583/150‑1 (MQ=255)
                                                                                                    aaaTTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGc                                             <  1:126651/150‑1 (MQ=255)
                                                                                                      aTTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCa                                           <  2:647611/150‑1 (MQ=255)
                                                                                                         gCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAAcc                                        <  2:407405/150‑1 (MQ=255)
                                                                                                            gACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACg                                     >  1:1375156/1‑150 (MQ=255)
                                                                                                                       aTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGt                          <  1:835671/150‑1 (MQ=255)
                                                                                                                       aTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGt                          <  1:184202/150‑1 (MQ=255)
                                                                                                                         ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTgg                        <  1:929861/150‑1 (MQ=255)
                                                                                                                         ttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGAATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTgg                        >  2:1096872/1‑150 (MQ=255)
                                                                                                                           gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGtt                      <  2:1589818/150‑1 (MQ=255)
                                                                                                                             tCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCTGCGGACGTGGTTgg                    <  2:1095116/150‑2 (MQ=255)
                                                                                                                             tCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGc                    <  1:1360463/150‑1 (MQ=255)
                                                                                                                             tCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCAACCACGTCTGCGGACGTGGTTggc                   <  1:592923/150‑3 (MQ=255)
                                                                                                                               aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTa                  >  1:267217/1‑150 (MQ=255)
                                                                                                                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTAt                 >  2:48081/2‑150 (MQ=25)
                                                                                                                                    ggataaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAg             >  2:1453235/7‑150 (MQ=25)
                                                                                                                                       taaggcgTTCACGCCGCATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATa          >  2:940772/4‑150 (MQ=25)
                                                                                                                                       tGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATa          >  2:274514/1‑150 (MQ=255)
                                                                                                                                         cggcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAAc        >  2:608550/1‑150 (MQ=255)
                                                                                                                                          ggcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAACCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACg       <  2:1133152/150‑1 (MQ=255)
                                                                                                                                            cgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTc     <  1:884941/150‑1 (MQ=255)
                                                                                                                                             gTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCg    <  2:694340/150‑1 (MQ=255)
                                                                                                                                               gAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGAt  >  1:1422124/1‑150 (MQ=255)
                                                                                                                                               gAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGAt  >  1:874425/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
ATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGAT  >  NC_000913/699296‑699588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: