Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 721,004 T→A 6.6% intergenic (+164/+52) ybfK → / ← kdpE uncharacterized protein/response regulator in two‑component regulatory system with KdpD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913721,0040TA 6.6% 352.6 / 9.7 119intergenic (+164/+52)ybfK/kdpEuncharacterized protein/response regulator in two‑component regulatory system with KdpD
Reads supporting (aligned to +/- strand):  ref base T (47/64);  new base A (3/5);  total (50/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 131 total aligned reads displayed.

AAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCATATA  >  NC_000913/720855‑721151
                                                                                                                                                     |                                                                                                                                                   
aatatatTCTATCCATGAAATAAGGTTGAAATTTGTATGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtat                                                                                                                                                     <  1:421970/147‑1 (MQ=255)
  tatatTCTATCCAGGAAATAATGTTTAACTGTGAAGGTGATAAATTAAATATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATTTAATATTTTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtattt                                                                                                                                                   <  1:251548/149‑1 (MQ=255)
  gATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtattt                                                                                                                                                   >  2:423479/1‑150 (MQ=255)
    tatTCTATCCAGGAAATACGGTTTAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatttat                                                                                                                                                 <  2:57859/150‑1 (MQ=255)
    tatTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatttat                                                                                                                                                 >  2:1139471/1‑150 (MQ=255)
     atTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatttatt                                                                                                                                                >  1:848483/1‑150 (MQ=255)
      ttCTATCCATGAAATACGGTTGAACTGTTAATGTTATCAATAATAAATGATCAATATTATTTTATTTAATGATGATCTTTTTACTCAGTAATATAATATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCAGTATttatta                                                                                                                                               <  2:176873/150‑1 (MQ=255)
      ttCTATCCAGGAAATACGGTTTAACTGTGAAGGTGATCAATAAAAAAAGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGATATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATttatta                                                                                                                                               <  2:1054308/150‑1 (MQ=255)
         tATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATttattatta                                                                                                                                            <  1:1467976/150‑1 (MQ=255)
            ccAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGATATTTTTGTGTGTTGTTTAAGATAAAAAGACGTATttattattaaag                                                                                                                                         >  1:80719/1‑147 (MQ=255)
              atgAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCt                                                                                                                                       <  1:18269/148‑1 (MQ=255)
              aGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCt                                                                                                                                       >  2:1481055/1‑150 (MQ=255)
              aGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCt                                                                                                                                       <  2:222077/150‑1 (MQ=255)
               ggAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCACTCTTAATTTATTTAATGATGAGCTGTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCTTATTTATTATTACGGCtt                                                                                                                                      >  2:267692/1‑150 (MQ=255)
               ggAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCtt                                                                                                                                      >  2:763879/1‑150 (MQ=255)
                gAAATTCGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCttt                                                                                                                                     <  2:170496/150‑1 (MQ=255)
                gAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCttt                                                                                                                                     <  2:83105/150‑1 (MQ=255)
                gAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCttt                                                                                                                                     <  2:223279/150‑1 (MQ=255)
                gAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCttt                                                                                                                                     <  2:51901/150‑1 (MQ=255)
                 ataTACTGTTGAACTGTGAAGGTGTTCAATTAATAATGATCAATCTTAATTTATTTAATGATGATGTTTTTAATCAGTAATTTAAAATTTTGTATTTTTATTTTTGTGTGTTTTTTAAGTTAAAAAGCCGTTATTATAAATACGGCtttt                                                                                                                                    <  2:568862/148‑2 (MQ=255)
                 aaaTACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTAATAATAAATACGGCtttt                                                                                                                                    <  2:1451331/150‑2 (MQ=255)
                 aaaTACGGTTGAACTGAGAAGGAGATCAATAAAAAATGATCAATCTTAATTTATTTTATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAACGCCGTAATAATAACTACGGCtttt                                                                                                                                    <  1:95654/150‑2 (MQ=255)
                    tACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaatt                                                                                                                                 <  2:1601294/150‑1 (MQ=255)
                      cGGTTGAACTGTGAAGGTGATCAATAAAAAATGATAAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTAATAATAAATACGGCtttttatct                                                                                                                               <  1:611203/150‑7 (MQ=255)
                       ggTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTtaattaat                                                                                                                              <  1:1377019/150‑1 (MQ=255)
                        gTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGACGTATTTATTATTACGGCTTTAATtaata                                                                                                                             >  1:1038018/1‑150 (MQ=255)
                           taaCTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATaaaa                                                                                                                          <  2:1706702/149‑1 (MQ=255)
                           gAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATaaaa                                                                                                                          >  2:631580/1‑150 (MQ=255)
                           gAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATAGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATaaaa                                                                                                                          >  1:181595/1‑150 (MQ=255)
                               tgtgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggca                                                                                                                      <  2:773176/150‑1 (MQ=255)
                                gtgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggcag                                                                                                                     >  1:1032796/1‑150 (MQ=255)
                                gtgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGACGTATTTATTATTACGGATTTAATTAATAAAaggcag                                                                                                                     >  1:978335/1‑150 (MQ=255)
                                 tgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggcagg                                                                                                                    <  1:1581042/150‑1 (MQ=255)
                                    aGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGATTTGTTATTTTTGTGAGTTGTGTTAGATAAAACGCCGTATTTATTATTACGGCTTTAAGTATTAAAAGGCAGTCtt                                                                                                                 >  2:300862/1‑149 (MQ=255)
                                     ggTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGACGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGt                                                                                                                >  1:911991/1‑150 (MQ=255)
                                      gTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTa                                                                                                               >  2:358724/1‑150 (MQ=255)
                                        taTCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTAtt                                                                                                             <  1:635105/149‑1 (MQ=255)
                                           cAATAAAAAATGGTCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTaaa                                                                                                          >  2:1587952/1‑150 (MQ=255)
                                            aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtattt                                                                                                                                                   <  2:455527/108‑1 (MQ=255)
                                            aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtattt                                                                                                                                                   >  1:455527/1‑108 (MQ=255)
                                            aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTa                                                                                                            >  2:61682/1‑147 (MQ=255)
                                            aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTa                                                                                                            <  1:61682/147‑1 (MQ=255)
                                               aaaaaaTGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTa                                                                                                      <  1:1531061/150‑1 (MQ=255)
                                               aaaaaaTGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTa                                                                                                      <  2:1148925/150‑1 (MQ=255)
                                                aaaaaTGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCATTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTaa                                                                                                     <  1:108263/150‑1 (MQ=255)
                                                       aTCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCaa                                                                                              <  2:1305327/150‑1 (MQ=255)
                                                         cAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAg                                                                                            <  1:1139471/150‑1 (MQ=255)
                                                             cTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATa                                                                                        >  1:839632/1‑150 (MQ=255)
                                                             cTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATa                                                                                        >  2:1381778/1‑150 (MQ=255)
                                                                aaTTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCTTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAc                                                                                     >  2:1510373/1‑150 (MQ=255)
                                                                 atttatttAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAcc                                                                                    <  1:1723766/150‑1 (MQ=255)
                                                                     atttaATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATa                                                                                >  2:891587/1‑150 (MQ=255)
                                                                     atttaATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATa                                                                                <  2:1418641/150‑1 (MQ=255)
                                                                     atttaATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATa                                                                                >  2:841692/1‑150 (MQ=255)
                                                                        taATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACAGATAGcc                                                                             >  1:1261040/1‑150 (MQ=255)
                                                                         aaTGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCa                                                                            >  2:113975/1‑150 (MQ=255)
                                                                           tgatgaGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAAt                                                                          <  1:1686217/150‑1 (MQ=255)
                                                                             atgaGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCTATAGCCAATAc                                                                        >  1:775575/1‑150 (MQ=255)
                                                                             atgaGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATAc                                                                        <  2:539079/150‑1 (MQ=255)
                                                                              tgaGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATAcc                                                                       <  1:137895/150‑1 (MQ=255)
                                                                               gaGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCg                                                                      <  2:195270/150‑1 (MQ=255)
                                                                                 gCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGt                                                                    >  1:1244760/1‑150 (MQ=255)
                                                                                    ttttACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTc                                                                 <  2:1020223/150‑1 (MQ=255)
                                                                                    ttttACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATAAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTc                                                                 <  2:592021/150‑1 (MQ=255)
                                                                                      ttACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAg                                                               <  1:358724/150‑1 (MQ=255)
                                                                                      ttACTCAGAAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAg                                                               <  1:309869/150‑1 (MQ=255)
                                                                                       tACTCATTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGt                                                              <  2:1063863/150‑1 (MQ=255)
                                                                                       tACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGt                                                              <  1:481696/150‑1 (MQ=255)
                                                                                         cTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTaa                                                            >  2:1273625/1‑150 (MQ=255)
                                                                                           cAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATg                                                          <  2:1442143/150‑1 (MQ=255)
                                                                                             ttAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAATGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGaa                                                        <  1:1710193/149‑1 (MQ=255)
                                                                                                  ataAAAAATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCTTGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGc                                                   >  2:1655238/1‑150 (MQ=255)
                                                                                                        tatTGAATTGTTATTTTTGTGTGTTGTTTAAGATTAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTgggc                                             <  2:513726/150‑1 (MQ=255)
                                                                                                         atTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTgggcg                                            >  1:1527801/1‑150 (MQ=255)
                                                                                                            gAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTgggcgggc                                         <  2:848483/150‑1 (MQ=255)
                                                                                                            gAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTgggcgggc                                         >  2:1319998/1‑150 (MQ=255)
                                                                                                             aaTTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGggcgggcg                                        <  1:1587952/150‑1 (MQ=255)
                                                                                                               ttGTTTTTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGcgggcggg                                      <  2:80719/150‑1 (MQ=255)
                                                                                                               ttGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGcgggcggg                                      <  2:591492/150‑1 (MQ=255)
                                                                                                               ttGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGcgggcggg                                      <  2:1032796/150‑1 (MQ=255)
                                                                                                                tGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAat                                                                                                    <  2:1255541/87‑1 (MQ=255)
                                                                                                                tGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAat                                                                                                    >  1:1255541/1‑87 (MQ=255)
                                                                                                                 gTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGAt                                    <  1:423479/150‑1 (MQ=255)
                                                                                                                   tATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCTGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATcc                                  <  1:936777/150‑1 (MQ=255)
                                                                                                                         tgtgtgTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTcc                            >  2:1398689/1‑150 (MQ=255)
                                                                                                                         tgtgtgTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTcc                            <  1:1319998/150‑1 (MQ=255)
                                                                                                                            gtgtTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGt                         >  1:595889/1‑150 (MQ=255)
                                                                                                                             tgttgtTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAACGATTGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCAAGtt                        >  2:68698/1‑150 (MQ=255)
                                                                                                                                   ttAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTc                  <  2:1354191/150‑1 (MQ=255)
                                                                                                                                   ttAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTc                  <  2:630753/150‑1 (MQ=255)
                                                                                                                                      aGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCa               >  1:1439175/1‑150 (MQ=255)
                                                                                                                                          aaaaaGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATg           >  2:776897/1‑150 (MQ=255)
                                                                                                                                           taaaGCCGTAATAATAAATACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTATTATTCAACGCATAAAACGTTAGCAAATACCGGTTTCAGTAATGAAATGGCTTGGGCGGGCGGCATCCTGTTCCCGTTTTTGTCGCAGATGt          >  1:330876/2‑150 (MQ=255)
                                                                                                                                            aaaGCCGTAATAATAAATACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTc         >  2:1414331/1‑150 (MQ=255)
                                                                                                                                                ccGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCTGGCTGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCat     <  1:775591/150‑1 (MQ=255)
                                                                                                                                                ccGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCat     <  1:1709742/150‑1 (MQ=255)
                                                                                                                                                 cGTATTTATTATTACTGATTTAATTAATAAATGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAACGATAGCCAATACGGGTTTAAGTAATGAACTGGGGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCata    <  2:1228866/150‑1 (MQ=25)
                                                                                                                                                  gTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCatat   <  1:841692/150‑1 (MQ=255)
                                                                                                                                                  gTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCatat   <  1:891587/150‑1 (MQ=255)
                                                                                                                                                   ttttttttATTACGGGTTTAATTAAAAAAAGGCATGCTTTATTAAAATTAAAATTCAAACCAAAAACCGAAACCCAAAACCGGTTTCATTAATGAAATGGGGGGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCAtata  <  2:386533/148‑1 (MQ=18)
                                                                                                                                                     |                                                                                                                                                   
AAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCATATA  >  NC_000913/720855‑721151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: