Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 721,006 T→A 6.1% intergenic (+166/+50) ybfK → / ← kdpE uncharacterized protein/response regulator in two‑component regulatory system with KdpD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913721,0060TA 6.1% 318.0 / 7.5 114intergenic (+166/+50)ybfK/kdpEuncharacterized protein/response regulator in two‑component regulatory system with KdpD
Reads supporting (aligned to +/- strand):  ref base T (46/61);  new base A (3/4);  total (49/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01

Only 100 of 130 total aligned reads displayed.

GATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCATATAAATA  >  NC_000913/720857‑721155
                                                                                                                                                     |                                                                                                                                                     
tatatTCTATCCAGGAAATAATGTTTAACTGTGAAGGTGATAAATTAAATATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTTAGTAATTTAATATTTTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtattt                                                                                                                                                       <  1:251548/149‑1 (MQ=255)
gATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtattt                                                                                                                                                       >  2:423479/1‑150 (MQ=255)
  tatTCTATCCAGGAAATACGGTTTAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatttat                                                                                                                                                     <  2:57859/150‑1 (MQ=255)
  tatTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatttat                                                                                                                                                     <  2:603475/150‑1 (MQ=255)
  tatTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatttat                                                                                                                                                     >  2:1139471/1‑150 (MQ=255)
   atTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGtatttatt                                                                                                                                                    >  1:848483/1‑150 (MQ=255)
    ttCTATCCATGAAATACGGTTGAACTGTTAATGTTATCAATAATAAATGATCAATATTATTTTATTTAATGATGATCTTTTTACTCAGTAATATAATATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCAGTATttatta                                                                                                                                                   <  2:176873/150‑1 (MQ=255)
    ttCTATCCAGGAAATACGGTTTAACTGTGAAGGTGATCAATAAAAAAAGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGATATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATttatta                                                                                                                                                   <  2:1054308/150‑1 (MQ=255)
      cTAACCAGGAAATACGGTTGAACTGTGAAGGTGATCAAAAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATttattatt                                                                                                                                                 <  2:1160843/150‑1 (MQ=255)
       tATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATttattatta                                                                                                                                                <  1:1467976/150‑1 (MQ=255)
          ccAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGATATTTTTGTGTGTTGTTTAAGATAAAAAGACGTATttattattaaag                                                                                                                                             >  1:80719/1‑147 (MQ=255)
            atgAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCt                                                                                                                                           <  1:18269/148‑1 (MQ=255)
            aGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCt                                                                                                                                           <  2:222077/150‑1 (MQ=255)
            aGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCt                                                                                                                                           >  2:1481055/1‑150 (MQ=255)
             ggAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCACTCTTAATTTATTTAATGATGAGCTGTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCTTATTTATTATTACGGCtt                                                                                                                                          >  2:267692/1‑150 (MQ=255)
             ggAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCtt                                                                                                                                          >  2:763879/1‑150 (MQ=255)
              taaaTACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCttt                                                                                                                                         <  2:1438718/149‑1 (MQ=255)
              gAAATTCGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCttt                                                                                                                                         <  2:170496/150‑1 (MQ=255)
              gAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCttt                                                                                                                                         <  2:83105/150‑1 (MQ=255)
              gAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCttt                                                                                                                                         <  2:461154/150‑1 (MQ=255)
              gAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCttt                                                                                                                                         <  2:51901/150‑1 (MQ=255)
               ataTACTGTTGAACTGTGAAGGTGTTCAATTAATAATGATCAATCTTAATTTATTTAATGATGATGTTTTTAATCAGTAATTTAAAATTTTGTATTTTTATTTTTGTGTGTTTTTTAAGTTAAAAAGCCGTTATTATAAATACGGCtttt                                                                                                                                        <  2:568862/148‑2 (MQ=255)
               aaaTACGGTTGAACTGTGAAGGTTATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTTAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTAATAATAAATCCGGCtttt                                                                                                                                        <  1:1530660/150‑2 (MQ=255)
               aaaTACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTAATAATAAATACGGCtttt                                                                                                                                        <  2:1451331/150‑2 (MQ=255)
               aaaTACGGTTGAACTGAGAAGGAGATCAATAAAAAATGATCAATCTTAATTTATTTTATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAACGCCGTAATAATAACTACGGCtttt                                                                                                                                        <  1:95654/150‑2 (MQ=255)
                  tACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaatt                                                                                                                                     <  2:1601294/150‑1 (MQ=255)
                    cGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTttaattaa                                                                                                                                   >  2:1029768/1‑150 (MQ=255)
                     ggTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTtaattaat                                                                                                                                  <  1:1377019/150‑1 (MQ=255)
                      gTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATtaata                                                                                                                                 >  2:1709742/1‑150 (MQ=255)
                      gTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGACGTATTTATTATTACGGCTTTAATtaata                                                                                                                                 >  1:1038018/1‑150 (MQ=255)
                         gAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATaaaa                                                                                                                              >  2:242984/1‑150 (MQ=255)
                             tgtgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggca                                                                                                                          <  2:773176/150‑1 (MQ=255)
                              gtgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAaggcag                                                                                                                         >  1:1032796/1‑150 (MQ=255)
                              gtgAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGACGTATTTATTATTACGGATTTAATTAATAAAaggcag                                                                                                                         >  1:978335/1‑150 (MQ=255)
                                  aGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGATTTGTTATTTTTGTGAGTTGTGTTAGATAAAACGCCGTATTTATTATTACGGCTTTAAGTATTAAAAGGCAGTCtt                                                                                                                     >  2:300862/1‑149 (MQ=255)
                                     tGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTAt                                                                                                                  >  2:1386030/1‑150 (MQ=255)
                                      taTCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTAtt                                                                                                                 <  1:635105/149‑1 (MQ=255)
                                         cAATAAAAAATGGTCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTaaa                                                                                                              >  2:1587952/1‑150 (MQ=255)
                                          aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTa                                                                                                                <  1:61682/147‑1 (MQ=255)
                                          aaTAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTa                                                                                                                >  2:61682/1‑147 (MQ=255)
                                             aaaaaaTGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTa                                                                                                          <  1:1531061/150‑1 (MQ=255)
                                              aaaaaTGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCATTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTaa                                                                                                         <  1:108263/150‑1 (MQ=255)
                                                    gATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATTAAAGGAAGGATTTATTAAAATTAATATTCa                                                                                                   >  1:285909/1‑150 (MQ=255)
                                                     aTCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCaa                                                                                                  <  2:1305327/150‑1 (MQ=255)
                                                       cAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAg                                                                                                <  1:1139471/150‑1 (MQ=255)
                                                           cTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATa                                                                                            >  1:839632/1‑150 (MQ=255)
                                                              aaTTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCTTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAc                                                                                         >  2:1510373/1‑150 (MQ=255)
                                                              aaTTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTATTTAATATAAGGCAGGATGTATTAAAATTAATATTCAAAGCATAAAc                                                                                         >  1:1725474/1‑150 (MQ=255)
                                                               atttatttAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAcc                                                                                        <  1:1723766/150‑1 (MQ=255)
                                                                   atttaATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATa                                                                                    >  2:841692/1‑150 (MQ=255)
                                                                   atttaATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATa                                                                                    >  2:891587/1‑150 (MQ=255)
                                                                      taATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGcc                                                                                 >  1:386533/1‑150 (MQ=255)
                                                                      taATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACAGATAGcc                                                                                 >  1:1261040/1‑150 (MQ=255)
                                                                       aaTGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCa                                                                                >  2:113975/1‑150 (MQ=255)
                                                                         tgatgaGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAAt                                                                              <  1:1686217/150‑1 (MQ=255)
                                                                           atgaGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCTATAGCCAATAc                                                                            >  1:775575/1‑150 (MQ=255)
                                                                           atgaGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATAc                                                                            <  2:539079/150‑1 (MQ=255)
                                                                            tgaGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATAcc                                                                           <  1:137895/150‑1 (MQ=255)
                                                                             gaGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCg                                                                          <  2:195270/150‑1 (MQ=255)
                                                                               gCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGt                                                                        >  1:1244760/1‑150 (MQ=255)
                                                                                    ttACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAg                                                                   <  1:358724/150‑1 (MQ=255)
                                                                                    ttACTCAGTAAAATAAAATATTTAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAg                                                                   <  2:1083349/150‑1 (MQ=255)
                                                                                    ttACTCAGAAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAg                                                                   <  1:309869/150‑1 (MQ=255)
                                                                                     tACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGt                                                                  >  1:576813/1‑150 (MQ=255)
                                                                                     tACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGt                                                                  <  1:481696/150‑1 (MQ=255)
                                                                                       cTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTaa                                                                >  2:1273625/1‑150 (MQ=255)
                                                                                        tCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAAt                                                               <  1:789106/150‑1 (MQ=255)
                                                                                         cAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATg                                                              <  2:1442143/150‑1 (MQ=255)
                                                                                           ttAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAATGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGaa                                                            <  1:1710193/149‑1 (MQ=255)
                                                                                             aaTATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAAt                                                          >  2:381876/1‑150 (MQ=255)
                                                                                                ataAAAAATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCTTGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGc                                                       >  2:1655238/1‑150 (MQ=255)
                                                                                                      tatTGAATTGTTATTTTTGTGTGTTGTTTAAGATTAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTgggc                                                 <  2:513726/150‑1 (MQ=255)
                                                                                                       atTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTgggcg                                                >  1:1527801/1‑150 (MQ=255)
                                                                                                         tGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTgggcggg                                              >  2:6410/1‑150 (MQ=255)
                                                                                                          gAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTgggcgggc                                             >  2:1319998/1‑150 (MQ=255)
                                                                                                          gAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTgggcgggc                                             <  2:848483/150‑1 (MQ=255)
                                                                                                           aaTTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGggcgggcg                                            <  1:1587952/150‑1 (MQ=255)
                                                                                                             ttGTTTTTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGcgggcggg                                          <  2:80719/150‑1 (MQ=255)
                                                                                                             ttGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGcgggcggg                                          <  2:591492/150‑1 (MQ=255)
                                                                                                             ttGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGcgggcggg                                          <  2:1032796/150‑1 (MQ=255)
                                                                                                              tGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAat                                                                                                        >  1:1255541/1‑87 (MQ=255)
                                                                                                              tGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAat                                                                                                        <  2:1255541/87‑1 (MQ=255)
                                                                                                               gTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGAt                                        <  1:423479/150‑1 (MQ=255)
                                                                                                                 tATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCTGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATcc                                      <  1:936777/150‑1 (MQ=255)
                                                                                                                 tATTTTTGGGGGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTAGTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTGCAGTAATAAAATGGCGTGGGCGGGCGGGATcc                                      <  1:626898/150‑1 (MQ=255)
                                                                                                                       tgtgtgTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTcc                                >  2:1398689/1‑150 (MQ=255)
                                                                                                                       tgtgtgTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTcc                                <  1:1319998/150‑1 (MQ=255)
                                                                                                                         tgtgtTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAg                              <  1:913312/150‑1 (MQ=255)
                                                                                                                          gtgtTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGt                             >  1:595889/1‑150 (MQ=255)
                                                                                                                           tgttgtTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAACGATTGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCAAGtt                            >  2:68698/1‑150 (MQ=255)
                                                                                                                               gttTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTg                        <  1:1697202/150‑1 (MQ=255)
                                                                                                                                 ttAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTc                      <  2:630753/150‑1 (MQ=255)
                                                                                                                                 ttAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTc                      <  2:1354191/150‑1 (MQ=255)
                                                                                                                                    aGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCAAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATACTTTTCAAGTTTTTGTCGCa                   >  1:847543/1‑150 (MQ=255)
                                                                                                                                        aaaaaGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATg               >  2:776897/1‑150 (MQ=255)
                                                                                                                                          aaaGCCGTAATAATAAATACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTc             >  2:1414331/1‑150 (MQ=255)
                                                                                                                                               cGTATTTATTATTACTGATTTAATTAATAAATGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAAACGATAGCCAATACGGGTTTAAGTAATGAACTGGGGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCata        <  2:1228866/150‑1 (MQ=25)
                                                                                                                                                gTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCatat       <  1:891587/150‑1 (MQ=255)
                                                                                                                                                 ttttttttATTACGGGTTTAATTAAAAAAAGGCATGCTTTATTAAAATTAAAATTCAAACCAAAAACCGAAACCCAAAACCGGTTTCATTAATGAAATGGGGGGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCAtata      <  2:386533/148‑1 (MQ=18)
                                                                                                                                                     tattattaCGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTATTGTCGCAGATGTCCCATATAAATa  >  2:162147/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAATATAAAATATTGAATTGTTATTTTTGTGTGTTGTTTAAGATAAAAAGCCGTATTTATTATTACGGCTTTAATTAATAAAAGGCAGGCTGTATTAAAATTAATATTCAAAGCATAAACCGATAGCCAATACCGGTTTCAGTAATGAAATGGCGTGGGCGGGCGGGATCCTGTTCCAGTTTTTGTCGCAGATGTCCCATATAAATA  >  NC_000913/720857‑721155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: