Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 729,376 G→T 8.0% intergenic (+36/‑207) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913729,3760GT 8.0% 285.9 / 8.3 111intergenic (+36/‑207)ybfA/rhsCDUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base G (50/51);  new base T (2/8);  total (52/59)
Fisher's exact test for biased strand distribution p-value = 1.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAA  >  NC_000913/729227‑729525
                                                                                                                                                     |                                                                                                                                                       
ttCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCgg                                                                                                                                                         >  1:1693028/1‑150 (MQ=255)
 tCTGGAAAGACCGCGACCGCTTTTACAGCTACCTGCATTGCGTATGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACAGCCAGCGGa                                                                                                                                                        <  2:331076/150‑1 (MQ=255)
  cTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCACCAGAGGaa                                                                                                                                                       >  1:1516339/1‑150 (MQ=255)
   tGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGaaa                                                                                                                                                      <  2:1004500/150‑1 (MQ=255)
   tGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGaaa                                                                                                                                                      <  1:1488806/150‑1 (MQ=255)
     gAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACg                                                                                                                                                    >  1:932371/1‑150 (MQ=255)
         gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTGTTGATTGCTGAGTTTTGAATACATTGAAAACCGCCATCCGAAACGCTgg                                                                                                                                                >  2:369754/1‑150 (MQ=255)
         gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                                >  1:775778/1‑150 (MQ=255)
           ccGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                                              <  2:1228295/150‑1 (MQ=255)
            cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCgg                                                                                                                                             <  1:931596/150‑1 (MQ=255)
            cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCgg                                                                                                                                             <  1:624796/150‑1 (MQ=255)
            cgcgCCCGCTTTTACAGCTACCGGCATCGCGGCTGGGCGAAAACCACCGATAAACCGGTGGGGATGGATCAGGCCGAAAAGGCAACTGCTGATTTTTATTGAATGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCgg                                                                                                                                             <  2:483267/150‑1 (MQ=255)
             gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAGCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGt                                                                                                                                            >  2:75167/1‑150 (MQ=255)
             gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGGCGAAAACCAGCGATAAACCGGTGTGGAGGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGt                                                                                                                                            <  1:1145721/150‑1 (MQ=255)
               gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGttt                                                                                                                                          <  1:1115459/150‑1 (MQ=255)
                cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGtttt                                                                                                                                         <  1:1260901/150‑1 (MQ=255)
                cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGtttt                                                                                                                                         <  2:1508451/150‑1 (MQ=255)
                 ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGttttt                                                                                                                                        <  1:1311868/150‑1 (MQ=255)
                  cGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCATCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtttttt                                                                                                                                       >  2:1112111/1‑150 (MQ=255)
                   gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCCGCGGAACCGCTGGCGGGTTTTTg                                                                                                                                      >  1:1523373/1‑150 (MQ=255)
                     ttttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGtt                                                                                                                                    >  1:1516706/1‑149 (MQ=255)
                     tgttACAGCGACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGttttcaatg                                                                                                                                    <  2:84050/148‑6 (MQ=255)
                        tACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCa                                                                                                                                 >  2:1692547/1‑150 (MQ=255)
                         aCAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGAGCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAg                                                                                                                                <  1:1447472/150‑1 (MQ=255)
                         aCAGCTACCTGCATCGCGTCCGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCCCCAGCGGAAACGCTGGCGGTTTTTTGTATCAg                                                                                                                                >  2:1272742/1‑150 (MQ=255)
                                 cTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCTACTGGAGATTTTTATTGATTGCTGAGTTTTGATTACATTGAAAAACGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGAAGt                                                                                                                        >  1:1117497/1‑150 (MQ=255)
                                  tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAAGTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTGTTTTGTATCAGGAAGATGtt                                                                                                                       >  1:262529/1‑150 (MQ=255)
                                   gcttCGCGTGAGGTCGAAAACCTGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGTTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTc                                                                                                                      <  2:1090530/147‑1 (MQ=255)
                                    cATCTCGTATGTTCTAAAACCAGCTATAAACGGGTGTGTTTGTATAAGGCCTAAAAGGCAACTGGGGATTTTTATTGATTGCTTAGTTTTGAATACATTGAAAACCGCAAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCa                                                                                                                     <  2:1517246/150‑1 (MQ=255)
                                       cgcgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTTAATACATTGAAAAACGCCAGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCacag                                                                                                                  >  2:1507282/1‑150 (MQ=255)
                                       cgcgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCagag                                                                                                                  <  2:915697/150‑1 (MQ=255)
                                       cgcgTCTGGTCGAAAACCAGCGATAAACCGGGGTGGATGGATCAGGCCTAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCagag                                                                                                                  <  1:1702699/150‑1 (MQ=255)
                                               gTCGAAAACCAGCGATAAACCGGTGTGGATGGAGCAGGCCGAAAGGCAACTGGTGATTATTATTGATTGCTGAGTTTTCAATACATTGCAAACCGCCAGCGGAACCGCTGGCGGTTTTTTGTATCAGGAAGATGTACAGAGGAATATTTa                                                                                                         >  1:231379/1‑150 (MQ=255)
                                                tCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGATGGCGGGTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                         >  1:265535/1‑150 (MQ=255)
                                                 cGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                                                        <  2:416891/150‑1 (MQ=255)
                                                 cGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAAACGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGCAAGATGTTAAGAGGAATATTTAg                                                                                                        >  2:497528/1‑150 (MQ=255)
                                                 cGAAAACCAGCGATAAACCGGGGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGCGTTTTGAAAACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                                                        <  2:1585195/150‑1 (MQ=255)
                                                  gAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGa                                                                                                       >  2:105054/1‑150 (MQ=255)
                                                  gAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGa                                                                                                       <  2:157847/150‑1 (MQ=255)
                                                   aaaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCTAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAc                                                                                                      <  2:1187550/150‑1 (MQ=255)
                                                   aaaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGCAACGCTGGCGGTTTTATGTAACACGAAGATGTTCAGAGGAATATTTAGAc                                                                                                      >  1:90258/1‑150 (MQ=255)
                                                      aCCAGCTATAAACCTGTGTGGATGGATCAGGCCGAAAAGTCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATc                                                                                                   <  1:1046311/150‑1 (MQ=255)
                                                      aCCAGCGATAAAGCGGTGTGGATGGAGCAGGCCGAATAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAAAACATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATc                                                                                                   <  2:856352/150‑1 (MQ=255)
                                                      aCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATc                                                                                                   >  2:1040654/1‑150 (MQ=255)
                                                        cAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                                                 >  1:1242538/1‑150 (MQ=255)
                                                        cAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGACAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                                                 >  1:349370/1‑150 (MQ=255)
                                                             aTAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAc                                                                                            <  1:681167/150‑1 (MQ=255)
                                                              tAAACCGGTGTGGATGTATCAGGCAGAAAAGTCAACTTGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACAGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           <  2:272286/150‑1 (MQ=255)
                                                               aaaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCa                                                                                          >  2:416298/1‑150 (MQ=255)
                                                                      tgtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaaa                                                                                   <  1:1160553/150‑1 (MQ=255)
                                                                      ggtgGATGGATCATTCCGAAAAGGAAACAGGTTATTTTAATTGTTTGCTTATTTTTGAATTCTTTGAAAACCGCCAGCGTAAACGGTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaaa                                                                                   <  1:1296094/149‑1 (MQ=255)
                                                                       gtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAAc                                                                                  <  2:1206121/150‑1 (MQ=255)
                                                                         ggatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACat                                                                                >  1:335768/1‑150 (MQ=255)
                                                                            tggatCAGGCCGAAAAGGCAACTGGGGATTTTTATTGATTGCTGAGTTTTTATTACTTTGATAACCGCAAGCGGAAACGCGGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatata                                                                             <  2:1555119/150‑1 (MQ=255)
                                                                              gatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAtt                                                                           >  2:565538/1‑150 (MQ=255)
                                                                                 cAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAt                                                                        >  1:55103/1‑150 (MQ=255)
                                                                                  aGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATg                                                                       <  1:1385224/150‑1 (MQ=255)
                                                                                  aGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCGGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATg                                                                       <  2:851085/150‑1 (MQ=255)
                                                                                     ccGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaa‑‑a                                                                  >  2:1379282/1‑150 (MQ=255)
                                                                                      cGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑at                                                                 <  1:135041/150‑1 (MQ=255)
                                                                                      cGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATATTTAGACATAGTTATACCAATCGAAACATATAATAATGaa‑‑aa                                                                 >  2:1668781/1‑149 (MQ=255)
                                                                                       gAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATTTTCATGAA‑‑ata                                                                >  2:1143609/1‑150 (MQ=255)
                                                                                        aaaaGGCAACTGGTGATTTTTATTGATTGCTGTGTTTTGAATTCATTGAAAACCGCCATCGGAATCTCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCTTGAA‑‑atat                                                               <  1:1507282/150‑1 (MQ=255)
                                                                                         aaaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑atata                                                              <  2:173547/150‑1 (MQ=255)
                                                                                            ggCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑atatatat                                                           >  1:1653650/1‑150 (MQ=255)
                                                                                            ggCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑atatatat                                                           >  2:237896/1‑150 (MQ=255)
                                                                                                  tGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAatat                                                     <  2:932371/150‑1 (MQ=255)
                                                                                                   ggTGATTTTTATTGATTGATGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGAGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATAtt                                                    >  1:31819/1‑150 (MQ=255)
                                                                                                     tGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATAtttt                                                  <  2:1693028/150‑1 (MQ=255)
                                                                                                      taTTTTTATTGATTGCTGAGTTTTGAATACATTAAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTc                                                 <  1:1272102/149‑1 (MQ=255)
                                                                                                      gATTTTTCTTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTc                                                 <  1:406134/150‑1 (MQ=255)
                                                                                                      gATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTc                                                 <  1:486066/150‑1 (MQ=255)
                                                                                                      gATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTc                                                 >  1:662453/1‑150 (MQ=255)
                                                                                                        tttttATTGATTGCTGCGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACCTCGTTATACCACTCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCt                                               <  2:265746/150‑1 (MQ=255)
                                                                                                        tttttATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCTGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATTTATATATAtttcatg                                               <  1:99913/150‑5 (MQ=255)
                                                                                                        tttttATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCt                                               <  1:416298/150‑1 (MQ=255)
                                                                                                         ttttATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTa                                              >  1:311367/1‑150 (MQ=255)
                                                                                                                gattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTATACATCGTTATAACAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTACTAATTGTTc                                       >  2:361905/1‑150 (MQ=255)
                                                                                                                 attgCTGAGTTTTTAATACATTGAAAACCGCCAGCTGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCt                                      <  1:1528188/150‑1 (MQ=255)
                                                                                                                 attgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCt                                      <  2:473331/150‑1 (MQ=255)
                                                                                                                         gTTTTGAATACATTGAAAACCGCCAGCGGAAACGCGGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑AAATATATAAATATTTTCCTAATTGTTCTTATATGaa                              >  1:1429746/1‑149 (MQ=255)
                                                                                                                               aaTACATTGAAAACCGCCAGCGGAAACGCGGGCGGGTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGatat                        >  1:163792/1‑150 (MQ=255)
                                                                                                                                aTACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtc                       <  1:1040654/150‑1 (MQ=255)
                                                                                                                                   cATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCa                    >  2:511709/1‑150 (MQ=255)
                                                                                                                                   cATTGAAAACCCCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATAATTAGACATCGTTATACAAAAAGAAACATAAATTCATGAA‑‑AAATATATAAATATTTACCTAATTGTTCTTATATTACAGATATATCa                    >  2:1705615/1‑150 (MQ=255)
                                                                                                                                      tGAAAACCGCCAGCTGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACtt                 <  1:717508/150‑1 (MQ=255)
                                                                                                                                       gAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTa                >  2:1133508/1‑150 (MQ=255)
                                                                                                                                        aaaaCCGCCAGCGTTTCCGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTaa               >  2:550544/1‑150 (MQ=255)
                                                                                                                                         aaaCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGGTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg              >  1:1721066/1‑150 (MQ=255)
                                                                                                                                          aaCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGTAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAgg             <  1:1112111/150‑1 (MQ=255)
                                                                                                                                             cGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCtt          >  2:813864/1‑150 (MQ=255)
                                                                                                                                              gCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCttt         >  2:540094/1‑150 (MQ=255)
                                                                                                                                              gCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCttt         >  2:615402/1‑150 (MQ=255)
                                                                                                                                               ccAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTc        <  2:1523373/150‑1 (MQ=255)
                                                                                                                                               ccAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTc        <  1:75167/150‑1 (MQ=255)
                                                                                                                                                cAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGAAATATAACTTAAGGCttt         >  1:1051095/1‑150 (MQ=255)
                                                                                                                                                cAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCt       <  2:775778/150‑1 (MQ=255)
                                                                                                                                                cAGCGGAAACGCGGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTTTACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCt       <  2:231379/150‑1 (MQ=255)
                                                                                                                                                 aGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCtt      <  2:1516706/150‑1 (MQ=255)
                                                                                                                                                     gAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATaa  >  1:1360732/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAA‑‑ATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAA  >  NC_000913/729227‑729525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: