Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 751,833 G→C 5.2% R113G (CGA→GGA)  ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913751,8330GC 5.2% 382.7 / 9.2 124R113G (CGA→GGA) ybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (64/52);  new base C (3/3);  total (69/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-01

Only 100 of 128 total aligned reads displayed.

AAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTT  >  NC_000913/751685‑751982
                                                                                                                                                    |                                                                                                                                                     
aaGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTTCCACGCCAGCCCGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCgg                                                                                                                                                      >  1:1054730/1‑150 (MQ=255)
 aGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCggg                                                                                                                                                     >  2:1731069/1‑150 (MQ=255)
     gTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACt                                                                                                                                                 >  1:541211/1‑150 (MQ=255)
      tAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTg                                                                                                                                                >  2:902372/1‑150 (MQ=255)
      tAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTg                                                                                                                                                <  1:1586427/150‑1 (MQ=255)
        aTCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGTAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTAATTTGCTGATTGGATTGATCGAAATTGAATAGCATTTCAGGTCTGGCAATGaa                                                                                                                                              >  1:1064276/1‑150 (MQ=255)
         tCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGaaa                                                                                                                                             <  1:1439760/150‑1 (MQ=255)
           cATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGATTACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATc                                                                                                                                           >  2:151649/1‑150 (MQ=255)
           cATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATc                                                                                                                                           >  1:1048007/1‑150 (MQ=255)
            aTCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGTTCCCGACCTGAAAtgc                                                                                                                                          <  1:637681/150‑3 (MQ=255)
            aTCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCa                                                                                                                                          <  1:1323257/150‑1 (MQ=255)
              cAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCaaa                                                                                                                                        >  2:644773/1‑149 (MQ=255)
               aGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTATTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATa                                                                                                                                       >  2:337075/1‑150 (MQ=255)
                ggACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGTTCCCGACCTGAAAtgctctt                                                                                                                                      <  1:1420208/150‑7 (MQ=255)
                 gACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGTTCCCGACCTGAAAtgctcttc                                                                                                                                     <  2:667090/150‑8 (MQ=255)
                 gACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATaca                                                                                                                                     >  1:1274670/1‑150 (MQ=255)
                   cacCGGCAACACCTTCTTTCCATGTAGAGGGGGTAGCCCAGTTTTCTGAGGGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTCGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACAcc                                                                                                                                   <  1:810141/150‑1 (MQ=255)
                       ggCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGCTCGAAATTGAAGCGCATTTCAGGTCGGGAACTGCAATCAATACCCCGatc                                                                                                                               >  1:1162975/1‑150 (MQ=255)
                       ggCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGatc                                                                                                                               <  1:1180220/150‑1 (MQ=255)
                       ggCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGatc                                                                                                                               <  2:352125/150‑1 (MQ=255)
                            cacCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGatctatct                                                                                                                          >  2:1562130/1‑150 (MQ=255)
                            cacCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCAGGAACTGAAATCAATACACCGatctatct                                                                                                                          >  2:170933/1‑150 (MQ=255)
                             acCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCtg                                                                                                                         >  2:1686054/1‑150 (MQ=255)
                              cgttcttTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAAACAATACTCCGATCTATCtgt                                                                                                                        >  2:443673/3‑150 (MQ=255)
                              ccTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCtgt                                                                                                                        <  2:1108261/150‑1 (MQ=255)
                              ccTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCtgt                                                                                                                        >  1:1352203/1‑150 (MQ=255)
                              ccTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTCATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCtgt                                                                                                                        >  1:482957/1‑150 (MQ=255)
                                     tCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAgg                                                                                                                 <  1:1216412/150‑1 (MQ=255)
                                      ccATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGATATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGa                                                                                                                >  1:1544452/1‑150 (MQ=255)
                                            agagGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACg                                                                                                          >  2:1372174/1‑150 (MQ=255)
                                            agagGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACg                                                                                                          >  1:471581/1‑150 (MQ=255)
                                             gagGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGTTCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACGGATCTAGCTGGGGCACGGACTGCCgg                                                                                                         >  2:1105687/1‑150 (MQ=255)
                                                 ggggAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGatat                                                                                                     >  2:747566/1‑150 (MQ=255)
                                                      gCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGt                                                                                                >  1:1382072/1‑150 (MQ=255)
                                                       cccAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGtt                                                                                               >  1:1173286/1‑150 (MQ=255)
                                                       cccAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAACGACTGACGGATTTCTGGtt                                                                                               <  1:247957/150‑1 (MQ=255)
                                                        ccAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGttt                                                                                              <  1:974653/150‑1 (MQ=255)
                                                        ccAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGttt                                                                                              <  1:816128/150‑1 (MQ=255)
                                                           gTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTaa                                                                                           >  1:1548601/1‑150 (MQ=255)
                                                             tttCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAAc                                                                                         <  2:1125811/150‑1 (MQ=255)
                                                             tttCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAAc                                                                                         >  1:776846/1‑150 (MQ=255)
                                                             tttCTGAGAGCCACGCCAGCCAGGCTTGCGGACTACTAATCTTCAGTGGCTGATTGGCCTGATCGAAATTGAAGAGCATTACATGTCGTGAATTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAAc                                                                                         <  2:1058973/150‑1 (MQ=255)
                                                                cTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCaa                                                                                      >  1:1435997/1‑150 (MQ=255)
                                                                   aGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAAtt                                                                                   <  2:1541173/150‑1 (MQ=255)
                                                                    gTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGATGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAAttt                                                                                  >  1:28777/1‑150 (MQ=255)
                                                                      gCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACCCCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTAt                                                                                >  2:944597/1‑150 (MQ=255)
                                                                      gCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGCCGGATATCTGGTTTTAAACCAAATTTAt                                                                                >  1:728543/1‑150 (MQ=255)
                                                                       ccACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATc                                                                               >  1:1254341/1‑150 (MQ=255)
                                                                       ccACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATc                                                                               >  2:513812/1‑150 (MQ=255)
                                                                           gccagccaGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGAcc                                                                           >  1:1097453/1‑150 (MQ=255)
                                                                             cagccagGCTTGCGGAATACTAATATTCAGTGGCTGAGTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTATTAAACCAAATTTATCGACCAg                                                                         >  2:641580/1‑150 (MQ=255)
                                                                              agccagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGt                                                                        <  1:1500379/150‑1 (MQ=255)
                                                                               gccagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTg                                                                       <  2:1009751/150‑1 (MQ=255)
                                                                                 ccggCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAACTCAATACACCGATCTATCTGTGGCAAGGACGGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGaa                                                                     >  2:153017/3‑150 (MQ=255)
                                                                                 cagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGaa                                                                     >  1:1235692/1‑150 (MQ=255)
                                                                                     cTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCAt                                                                 >  2:1043682/1‑150 (MQ=255)
                                                                                        gCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGa                                                              <  2:776846/150‑1 (MQ=255)
                                                                                          ggAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATg                                                            <  2:225083/150‑1 (MQ=255)
                                                                                           gAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGc                                                           <  2:1015019/150‑1 (MQ=255)
                                                                                            aaTACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCa                                                          <  2:548090/150‑1 (MQ=255)
                                                                                            aaTACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCa                                                          <  2:541211/150‑1 (MQ=255)
                                                                                               aCTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACAGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATgg                                                       <  2:562205/150‑1 (MQ=255)
                                                                                                cTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGa                                                      >  1:1443329/1‑150 (MQ=255)
                                                                                                  aaTATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGCCGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAAt                                                    >  2:124494/1‑150 (MQ=255)
                                                                                                   atatTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATg                                                   <  2:467758/150‑1 (MQ=255)
                                                                                                      ttCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGtt                                                <  1:1731069/150‑1 (MQ=255)
                                                                                                       tCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGttt                                               <  2:349554/150‑1 (MQ=255)
                                                                                                         aGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTg                                             <  1:170933/150‑1 (MQ=255)
                                                                                                          gTTGCTGATTGGCTTGATCGATATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGt                                            >  1:29124/1‑150 (MQ=255)
                                                                                                           ttGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTc                                           <  1:880646/150‑1 (MQ=255)
                                                                                                           ttGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTc                                           <  1:645508/150‑1 (MQ=255)
                                                                                                             gCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGCCTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCAc                                         <  1:1358354/150‑1 (MQ=255)
                                                                                                               tGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAATTTTATCGAGCAGTGAATATTTGATGCATGGATTGGTTTTGTccacc                                       >  2:875925/1‑146 (MQ=255)
                                                                                                                gATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGAt                                                             >  2:674408/1‑127 (MQ=255)
                                                                                                                gATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGAt                                                             <  1:674408/127‑1 (MQ=255)
                                                                                                                  ttGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACAAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCAttt                                    >  1:349756/1‑150 (MQ=255)
                                                                                                                     gCTTGATCGATATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCTATCTATCTGTGGCAAGGTCTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCtttttt                                 <  1:370820/150‑1 (MQ=255)
                                                                                                                     gCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGTTCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCtttttt                                 <  1:1559212/150‑1 (MQ=255)
                                                                                                                     gCTTGATAGAAATTGAAGAGCATTTCAGGTCGGGACCTGATATCATTACACCGATCTATCTGTGGCAAGGACTGACGGATATATGGTTTTAAACAAAATTTATCGAGCAGTGAATCATTGATGCTGGGAAGGGTTTTGTCACGCtttttt                                 >  2:80211/1‑150 (MQ=255)
                                                                                                                           tCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAg                           >  1:1394525/1‑150 (MQ=255)
                                                                                                                           tCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGAGCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAg                           <  1:867847/150‑1 (MQ=255)
                                                                                                                            cGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGt                          >  1:1372550/1‑150 (MQ=255)
                                                                                                                            cGAAATTGAAGAGCATTTCAGGACGGGAACTGAAATCAATACACCGATCTATCTGTGTCAAGGACTGACGGATATATGGTTTTAAAACAAATTTATCGCCCAGTGAATCATTGATGCCTGGAATGGTTTTGTCACACTTTTTTTGACAGt                          >  1:999680/1‑150 (MQ=255)
                                                                                                                             gataTTGAACAGCATTTCAGGTCGGGATCTGAAATCAATACACCGATCTTTCTGTGTCAAGGACTGACGGATATCTGGTTTTAAACAAAATTTATCGACCAGTTAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGtt                         <  1:337075/147‑1 (MQ=255)
                                                                                                                              aaaTTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTa                        >  2:1437897/1‑150 (MQ=255)
                                                                                                                              aaaTTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTa                        <  2:10801/150‑1 (MQ=255)
                                                                                                                              aaaTTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTa                        >  2:1021493/1‑150 (MQ=255)
                                                                                                                                  tGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTGAAttt                    >  1:1272412/1‑150 (MQ=255)
                                                                                                                                   gAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAAtttt                   >  1:222599/1‑150 (MQ=255)
                                                                                                                                   gAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAAtttt                   >  1:899617/1‑150 (MQ=255)
                                                                                                                                    aaGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGTTTTTGTCAACATTTTTTTGACAGTTAAttttt                  >  1:1053510/1‑150 (MQ=255)
                                                                                                                                    aaGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCAACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAAttttt                  <  2:1176210/150‑1 (MQ=255)
                                                                                                                                       agCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGc               <  2:459775/150‑1 (MQ=255)
                                                                                                                                       agCATTACAGGTCGTGAACTGAAATCAATCCACCGATCTATCTGTGGCAAGGACTGACGGATATCTTGTTTTAAACCAAATATATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGc               <  2:142557/150‑1 (MQ=255)
                                                                                                                                        tgaTTTCAGTTCCCGACCTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGcc              >  2:423122/3‑150 (MQ=255)
                                                                                                                                          aTTTCAGTTCCCGACCTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCa            >  1:1137599/1‑150 (MQ=255)
                                                                                                                                          aTTTCAGTTCCCGACCTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCa            >  1:1131798/1‑150 (MQ=255)
                                                                                                                                                 gTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGa     <  1:366367/150‑1 (MQ=255)
                                                                                                                                                 gTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGa     >  2:1456276/1‑150 (MQ=255)
                                                                                                                                                    gggAACTGAAATCAATACACCGATCTATCTGTGGCAATGACTGACGGATATCTGGGTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGAttt  <  2:1162975/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAGTTGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTT  >  NC_000913/751685‑751982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: