Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 751,837 A→C 5.3% S111R (AGT→AGG ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913751,8370AC 5.3% 358.0 / 8.1 125S111R (AGT→AGGybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (65/52);  new base C (4/3);  total (69/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

Only 100 of 127 total aligned reads displayed.

TGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTT  >  NC_000913/751689‑751986
                                                                                                                                                    |                                                                                                                                                     
tGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAAc                                                                                                                                                      <  2:1416677/150‑1 (MQ=255)
 gTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACt                                                                                                                                                     >  1:548090/1‑150 (MQ=255)
 gTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACt                                                                                                                                                     >  1:541211/1‑150 (MQ=255)
 gTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACt                                                                                                                                                     <  1:769680/150‑1 (MQ=255)
  tAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTg                                                                                                                                                    >  2:902372/1‑150 (MQ=255)
  tAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTg                                                                                                                                                    <  1:1586427/150‑1 (MQ=255)
   aaTCCCTCAGGACACCGGCACCACCTTCTTTCCAGGTAGAGGGGGGAGCCCAGTTGTCTGAGTGCCCCGCCCGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGCTTGGCTTGCTCGAAATTGAAGAGCATTGCAGGTCGGGAACGGa                                                                                                                                                   >  2:670823/1‑150 (MQ=255)
    aTCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGTAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTAATTTGCTGATTGGATTGATCGAAATTGAATAGCATTTCAGGTCTGGCAATGaa                                                                                                                                                  >  1:1064276/1‑150 (MQ=255)
     tCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGaaa                                                                                                                                                 >  2:478995/1‑150 (MQ=255)
       cATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATc                                                                                                                                               >  1:1048007/1‑150 (MQ=255)
        aTCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGTTCCCGACCTGAAAtgc                                                                                                                                              <  1:637681/150‑3 (MQ=255)
          gaGGACACCGTCAAGAGCTTCTTTCGATGTAGAGGGGGGAGCCCAGTTTTCTGAGTTCCAGGCCAGGCAGGCTTGCGGAATAGTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAAt                                                                                                                                            <  2:1028263/149‑1 (MQ=255)
          cAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCaaa                                                                                                                                            >  2:644773/1‑149 (MQ=255)
           aGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTATTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATa                                                                                                                                           >  2:337075/1‑150 (MQ=255)
            ggACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGTTCCCGACCTGAAAtgctctt                                                                                                                                          <  1:1420208/150‑7 (MQ=255)
             gACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGTTCCCGACCTGAAAtgctcttc                                                                                                                                         <  2:667090/150‑8 (MQ=255)
             gACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATaca                                                                                                                                         >  1:1274670/1‑150 (MQ=255)
               cacCGGCAACACCTTCTTTCCATGTAGAGGGGGTAGCCCAGTTTTCTGAGGGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTCGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACAcc                                                                                                                                       <  1:810141/150‑1 (MQ=255)
                   ggCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGCTCGAAATTGAAGCGCATTTCAGGTCGGGAACTGCAATCAATACCCCGatc                                                                                                                                   >  1:1162975/1‑150 (MQ=255)
                   ggCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGatc                                                                                                                                   <  2:352125/150‑1 (MQ=255)
                   ggCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGatc                                                                                                                                   <  1:1180220/150‑1 (MQ=255)
                        cacCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCAGGAACTGAAATCAATACACCGatctatct                                                                                                                              >  2:170933/1‑150 (MQ=255)
                         acCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCtg                                                                                                                             >  2:1686054/1‑150 (MQ=255)
                          cgttcttTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAAACAATACTCCGATCTATCtgt                                                                                                                            >  2:443673/3‑150 (MQ=255)
                          ccTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCtgt                                                                                                                            <  2:1108261/150‑1 (MQ=255)
                          ccTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCtgt                                                                                                                            >  1:1352203/1‑150 (MQ=255)
                                ttCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAg                                                                                                                      >  2:117060/1‑150 (MQ=255)
                                 tCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAgg                                                                                                                     <  1:1216412/150‑1 (MQ=255)
                                     tGTAGAGGGGGGAGCCCAGTTTTCTGAGTCCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTg                                                                                                                 >  1:10801/1‑150 (MQ=255)
                                        agagGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACg                                                                                                              >  1:471581/1‑150 (MQ=255)
                                        agagGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACg                                                                                                              <  2:1521624/150‑1 (MQ=255)
                                         gagGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGTTCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACGGATCTAGCTGGGGCACGGACTGCCgg                                                                                                             >  2:1105687/1‑150 (MQ=255)
                                             ggggAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGatat                                                                                                         >  2:747566/1‑150 (MQ=255)
                                                  gCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGt                                                                                                    >  1:1229031/1‑150 (MQ=255)
                                                   cccAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAACGACTGACGGATTTCTGGtt                                                                                                   <  1:247957/150‑1 (MQ=255)
                                                    ccAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGttt                                                                                                  <  1:974653/150‑1 (MQ=255)
                                                       gTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTaa                                                                                               >  1:998425/1‑150 (MQ=255)
                                                       gTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTaa                                                                                               >  1:1548601/1‑150 (MQ=255)
                                                         tttCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAAc                                                                                             >  1:776846/1‑150 (MQ=255)
                                                         tttCTGAGAGCCACGCCAGCCAGGCTTGCGGACTACTAATCTTCAGTGGCTGATTGGCCTGATCGAAATTGAAGAGCATTACATGTCGTGAATTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAAc                                                                                             <  2:1058973/150‑1 (MQ=255)
                                                            cTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCaa                                                                                          >  1:1435997/1‑150 (MQ=255)
                                                               aGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAAtt                                                                                       <  2:1541173/150‑1 (MQ=255)
                                                                gTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGATGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAAttt                                                                                      >  1:28777/1‑150 (MQ=255)
                                                                  gCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACCCCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTAt                                                                                    >  2:944597/1‑150 (MQ=255)
                                                                  gCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGCCGGATATCTGGTTTTAAACCAAATTTAt                                                                                    >  1:728543/1‑150 (MQ=255)
                                                                  gCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATTTGGTTTAAAACCAGAtatcc                                                                                    <  2:1672658/150‑5 (MQ=255)
                                                                    cACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCg                                                                                  >  1:1362959/1‑150 (MQ=255)
                                                                         cagccagGCTTGCGGAATACTAATATTCAGTGGCTGAGTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTATTAAACCAAATTTATCGACCAg                                                                             >  2:641580/1‑150 (MQ=255)
                                                                          agccagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGt                                                                            <  1:1500379/150‑1 (MQ=255)
                                                                           gccagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTg                                                                           <  2:1009751/150‑1 (MQ=255)
                                                                             ccggCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAACTCAATACACCGATCTATCTGTGGCAAGGACGGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGaa                                                                         >  2:153017/3‑150 (MQ=255)
                                                                             cagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGaa                                                                         >  1:1235692/1‑150 (MQ=255)
                                                                             cagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGaa                                                                         <  1:117060/150‑1 (MQ=255)
                                                                                 cTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCAt                                                                     >  2:1043682/1‑150 (MQ=255)
                                                                                    gCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGa                                                                  <  2:776846/150‑1 (MQ=255)
                                                                                     cGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGAt                                                                 >  1:335754/1‑150 (MQ=255)
                                                                                      ggAATACTAATATTCAGATGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTTAAATCAATAAACCTAGCTATCTGTTGCAATGACTGACGGCGATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATg                                                                <  1:835861/150‑1 (MQ=255)
                                                                                        aaTACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCa                                                              <  2:541211/150‑1 (MQ=255)
                                                                                        aaTACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCa                                                              <  2:548090/150‑1 (MQ=255)
                                                                                           aCTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACAGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATgg                                                           <  2:562205/150‑1 (MQ=255)
                                                                                            cTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGa                                                          >  1:1443329/1‑150 (MQ=255)
                                                                                              aaTATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGCCGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAAt                                                        >  2:124494/1‑150 (MQ=255)
                                                                                               atatTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATg                                                       <  2:467758/150‑1 (MQ=255)
                                                                                                   tCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGttt                                                   <  2:349554/150‑1 (MQ=255)
                                                                                                     aGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTg                                                 <  1:170933/150‑1 (MQ=255)
                                                                                                       ttGCTGATTGTCTTGATCGAAATTGAAGAGCATTTCATGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCTAGGACTGACGGATATCTGGTTTTAATCCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGTTTTTGTc                                               <  1:373095/150‑1 (MQ=255)
                                                                                                       ttGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTc                                               <  1:880646/150‑1 (MQ=255)
                                                                                                       ttGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTc                                               <  1:645508/150‑1 (MQ=255)
                                                                                                        tGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCa                                              <  2:1097453/150‑1 (MQ=255)
                                                                                                         gCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGCCTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCAc                                             <  1:1358354/150‑1 (MQ=255)
                                                                                                           tGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAATTTTATCGAGCAGTGAATATTTGATGCATGGATTGGTTTTGTccacc                                           >  2:875925/1‑146 (MQ=255)
                                                                                                              ttGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACAAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCAttt                                        >  1:349756/1‑150 (MQ=255)
                                                                                                                 gCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGTTCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCtttttt                                     <  1:1559212/150‑1 (MQ=255)
                                                                                                                 gCTTGATAGAAATTGAAGAGCATTTCAGGTCGGGACCTGATATCATTACACCGATCTATCTGTGGCAAGGACTGACGGATATATGGTTTTAAACAAAATTTATCGAGCAGTGAATCATTGATGCTGGGAAGGGTTTTGTCACGCtttttt                                     >  2:80211/1‑150 (MQ=255)
                                                                                                                      aTCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCAGTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCa                                >  1:143544/1‑150 (MQ=255)
                                                                                                                       tCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAg                               >  1:1394525/1‑150 (MQ=255)
                                                                                                                       tCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGAGCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAg                               <  1:867847/150‑1 (MQ=255)
                                                                                                                        cGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGt                              >  1:1372550/1‑150 (MQ=255)
                                                                                                                        cGAAATTGAAGAGCATTTCAGGACGGGAACTGAAATCAATACACCGATCTATCTGTGTCAAGGACTGACGGATATATGGTTTTAAAACAAATTTATCGCCCAGTGAATCATTGATGCCTGGAATGGTTTTGTCACACTTTTTTTGACAGt                              >  1:999680/1‑150 (MQ=255)
                                                                                                                         gataTTGAACAGCATTTCAGGTCGGGATCTGAAATCAATACACCGATCTTTCTGTGTCAAGGACTGACGGATATCTGGTTTTAAACAAAATTTATCGACCAGTTAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGtt                             <  1:337075/147‑1 (MQ=255)
                                                                                                                          aaaTTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTa                            >  2:1021493/1‑150 (MQ=255)
                                                                                                                          aaaTTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTa                            >  2:1437897/1‑150 (MQ=255)
                                                                                                                              tGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTGAAttt                        >  1:1272412/1‑150 (MQ=255)
                                                                                                                               gAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAAtttt                       >  1:222599/1‑150 (MQ=255)
                                                                                                                                aaGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGTTTTTGTCAACATTTTTTTGACAGTTAAttttt                      >  1:1053510/1‑150 (MQ=255)
                                                                                                                                aaGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCAACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAAttttt                      <  2:1176210/150‑1 (MQ=255)
                                                                                                                                   agCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGc                   <  2:459775/150‑1 (MQ=255)
                                                                                                                                   agCATTACAGGTCGTGAACTGAAATCAATCCACCGATCTATCTGTGGCAAGGACTGACGGATATCTTGTTTTAAACCAAATATATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGc                   <  2:142557/150‑1 (MQ=255)
                                                                                                                                    tgaTTTCAGTTCCCGACCTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGcc                  >  2:423122/3‑150 (MQ=255)
                                                                                                                                      aTTTCAGTTCCCGACCTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCa                >  1:1131798/1‑150 (MQ=255)
                                                                                                                                      aTTTCAGTTCCCGACCTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCa                >  1:1137599/1‑150 (MQ=255)
                                                                                                                                          cAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTaa            >  1:1451401/1‑149 (MQ=255)
                                                                                                                                          cAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTAc            <  1:1291733/150‑1 (MQ=255)
                                                                                                                                             gTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGa         <  1:366367/150‑1 (MQ=255)
                                                                                                                                             gTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGa         >  2:1456276/1‑150 (MQ=255)
                                                                                                                                               cGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCAATTACTGAtt       >  1:239101/1‑150 (MQ=255)
                                                                                                                                                gggAACTGAAATCAATACACCGATCTATCTGTGGCAATGACTGACGGATATCTGGGTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGAttt      <  2:1162975/150‑1 (MQ=255)
                                                                                                                                                 ggAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGAtttt     <  2:1548601/150‑1 (MQ=255)
                                                                                                                                                   aaCTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgt   >  1:382473/1‑150 (MQ=255)
                                                                                                                                                    aCTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgtt  >  2:686797/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGTAATCCATCAGGACACCGGCAACACCTTCTTTCCATGTAGAGGGGGGAGCCCAGTTTTCTGAGTGCCACGCCAGCCAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTT  >  NC_000913/751689‑751986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: