Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 775,613 T→C 5.0% I154T (ATC→ACC)  tolQ → membrane spanning protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913775,6130TC 5.0% 299.6 / 4.6 99I154T (ATC→ACC) tolQmembrane spanning protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base T (47/47);  new base C (2/3);  total (49/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

Only 100 of 101 total aligned reads displayed.

AGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAAC  >  NC_000913/775464‑775762
                                                                                                                                                     |                                                                                                                                                     
aGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTAt                                                                                                                                                       <  2:1658607/150‑1 (MQ=255)
  gggcaGGGGCGTCGCGTGCTATGCGTATCTCCATGCACCGTGAACTTGAAAATCTGGACACGCACATTCCGGTCAGCGGTACGGTTGTCTCCATCAGCCCGTATATTGTTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCg                                                                                                                                                     <  1:292452/146‑1 (MQ=255)
  gggAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCg                                                                                                                                                     <  1:1396129/149‑1 (MQ=255)
   ggAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGc                                                                                                                                                    >  2:1016380/1‑150 (MQ=255)
        gggCGTCGCGTGCTATGCGTATCTCCATGACCCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCGGGGGGATCATGCACGCCTTTATCGCCCTCg                                                                                                                                               >  1:130223/1‑150 (MQ=255)
            gTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGc                                                                                                                                           <  1:989828/150‑1 (MQ=255)
            gTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGc                                                                                                                                           <  1:1393923/150‑1 (MQ=255)
               gcgTGCTATGCGTATCTCCATGAACCGGGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGt                                                                                                                                        <  1:576897/150‑1 (MQ=255)
                ggTGCTATGCGTATCTGCATGAACCGTGATCTTGAAAATCTGGAAACGCAGATTCCGTTCCGCGGTACGGGTGGCTCCCTCACCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTa                                                                                                                                       <  1:897335/149‑1 (MQ=255)
                 ttGCTATGCGTATCTCCCTGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTaa                                                                                                                                      <  1:1674887/149‑1 (MQ=255)
                   gCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGGATATTGGTCTGGTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTaaaa                                                                                                                                    >  1:1603733/1‑150 (MQ=255)
                     tATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAACCGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACa                                                                                                                                  <  2:592572/150‑1 (MQ=255)
                     tATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACa                                                                                                                                  <  2:546992/150‑1 (MQ=255)
                      aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACaa                                                                                                                                 >  2:129354/1‑150 (MQ=255)
                      aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTACCGCCCCGAGGGCGATaaaggcg                                                                                                                                 <  1:1410975/150‑5 (MQ=255)
                      aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTCTGGTACGGTCTGGGGGATCATGCACGCCTTTACCGCCCCGAGGGCGATaaaggcg                                                                                                                                 <  1:1449618/150‑5 (MQ=255)
                       tGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAg                                                                                                                                >  2:395692/1‑150 (MQ=255)
                       tGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAg                                                                                                                                <  1:195809/150‑1 (MQ=255)
                        gCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGc                                                                                                                               <  2:1649605/150‑1 (MQ=255)
                        gCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGc                                                                                                                               <  2:1364818/150‑1 (MQ=255)
                          gTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGc                                                                                                                                           <  1:947065/136‑1 (MQ=255)
                           tATCTCCATGATCCGTGATCTTGAAAATCTGGAAACGCACATTCCGGTCCTGGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGGTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAac                                                                                                                            <  2:662946/150‑1 (MQ=255)
                           tATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAac                                                                                                                            <  1:1480412/150‑1 (MQ=255)
                           tATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGGCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAac                                                                                                                            <  2:1325437/150‑1 (MQ=255)
                            aTCTCCATGAACCGTGAAATTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTCGGACGGGCTGGGGGATCATGCACGCCTTGATCGCCGTCGGGGGGGTAAGACAAggacca                                                                                                                           >  1:289406/1‑145 (MQ=255)
                               tcCATGAACCGTGAACTTGAAAATCTGGTAACGCACATTCCGGTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGGTTGCTACGGGCTGGGGGATCATGCACGCCTTTATCGCCCTAGGGGCGGTAAAACAATCATCACtc                                                                                                                        >  1:250324/1‑149 (MQ=255)
                               tcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTg                                                                                                                        >  1:319662/1‑150 (MQ=255)
                                ccATGAACCGTGAACTTGAAAATCTGGAAACGCACCTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACTGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGc                                                                                                                       <  1:1542114/150‑1 (MQ=255)
                                      aCCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATgg                                                                                                                 >  1:1528764/1‑150 (MQ=255)
                                       ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGt                                                                                                                >  1:991382/1‑150 (MQ=255)
                                         gTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTg                                                                                                              <  2:1133357/150‑1 (MQ=255)
                                         gTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTg                                                                                                              <  2:319662/150‑1 (MQ=255)
                                            aaCTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTgcgc                                                                                                           >  1:1038032/1‑150 (MQ=255)
                                              cTTGAAAATCTGGAACCGCACATTCCGTTCCTAGGGACGGTTGGCTCCCTCAGCCCTTATATTGGTCTGTTTGGTACGGTATGGGGGATCGAGCACGACTTTTTCGCACGCGGGGCGGGAACACAAGCAACACGGCAAATGGTTgcgcac                                                                                                         >  2:1141754/1‑148 (MQ=255)
                                               ttGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCg                                                                                                        <  1:1016380/150‑1 (MQ=255)
                                                       cTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCa                                                                                                <  1:259515/150‑1 (MQ=255)
                                                             aCGCACATTCCGTTCCTCGGGACGGTTGGCTCCATCAGCCAGTATATTGGTCTGTTTGGTACGGGCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCg                                                                                          <  1:1157967/150‑1 (MQ=255)
                                                              cGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGt                                                                                         >  2:664893/1‑150 (MQ=255)
                                                              cGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGt                                                                                         >  1:1533550/1‑150 (MQ=255)
                                                               gCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGtt                                                                                        <  2:425989/150‑1 (MQ=255)
                                                                cacaTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATAGTGGTCTGTTTGGTACTGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTg                                                                                       <  2:1608953/150‑1 (MQ=255)
                                                                 acaTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGa                                                                                      >  1:871766/1‑150 (MQ=255)
                                                                     tCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGc                                                                                  <  1:996879/150‑1 (MQ=255)
                                                                       cGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTCTCGCAGAAGCGTTGATTGCgc                                                                                >  2:1522027/1‑149 (MQ=255)
                                                                        gTTCCTCGGTACGGGTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGAc                                                                               <  1:689170/150‑1 (MQ=255)
                                                                        gTTCCGCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGAc                                                                               <  1:986649/150‑1 (MQ=255)
                                                                          tcatCGGGACGGCTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATTCACTCCGTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTg                                                                             <  1:1483803/147‑1 (MQ=255)
                                                                           ccTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGc                                                                            >  1:808403/1‑150 (MQ=255)
                                                                           ccTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCGTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGc                                                                            <  2:1717666/150‑1 (MQ=255)
                                                                              cGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAAt                                                                         >  1:1257754/1‑150 (MQ=255)
                                                                                 tACGGGTGGCTCCCTCAGCCCGTATATTGGTCTGTTTGGTACGGTCGGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTgg                                                                      <  1:692330/150‑1 (MQ=255)
                                                                                      ttGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGTGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGt                                                                 <  2:935123/150‑1 (MQ=255)
                                                                                       gggCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGtt                                                                <  2:383141/149‑1 (MQ=255)
                                                                                         gCTCCATCAGCCCGTTTTTTGGTGTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAATACTAGCAACTCTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTg                                                              <  1:1588508/150‑1 (MQ=255)
                                                                                           tCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATAGCCCTCGGGGCGGTAAAACAAGCACCACTGCAAATGGTTGCGCCCGGTATCGCAGCAGCGGTGAATGCGACTGCAATTGGTATGTTAGcc                                                            >  1:135951/1‑150 (MQ=255)
                                                                                           gccATCAGCCCGTATATTTGTCTGTTTGGTACGGTCGGGGGGATCATGCACGCCTTTATCGCCCGCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGcc                                                            <  2:773452/149‑1 (MQ=255)
                                                                                                   cccGTATATTGGTCTGGTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCgggatagc                                                    <  1:918659/150‑8 (MQ=255)
                                                                                                          atTGGTGTGTTTGGTACGGTCTGGGGGATCATGCACGCGTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGGTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGtt                                             <  2:176707/150‑1 (MQ=255)
                                                                                                          atTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGtt                                             <  2:794491/150‑1 (MQ=255)
                                                                                                            tGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTAt                                           >  2:607175/1‑150 (MQ=255)
                                                                                                             ggTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCATATGGTTGCGCCGGGTATCGCTGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTTTg                                          >  1:1496876/1‑150 (MQ=255)
                                                                                                              gtttGTTTGGTACGGGCTGGGGGAGCATGCACGCATTTCTCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATgg                                         <  2:1083138/147‑1 (MQ=255)
                                                                                                              gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGTAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATgg                                         >  1:998523/1‑150 (MQ=255)
                                                                                                              gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATgg                                         <  2:371568/150‑1 (MQ=255)
                                                                                                                cTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCATATGGTTTAGCCCTGTATCGCAGAAGAGTTGATTGCGACTGAAATTGGTCTGTTTGCCGCTAGCCCGGCAGTTATGGcc                                       >  1:1062549/1‑150 (MQ=255)
                                                                                                                  gTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATGCCGGCAGTTATGGCCTa                                     >  2:739627/1‑150 (MQ=255)
                                                                                                                  gTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTa                                     >  1:1067422/1‑150 (MQ=255)
                                                                                                                  gTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTa                                     >  2:1250849/1‑150 (MQ=255)
                                                                                                                  gTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTa                                     >  2:739628/1‑150 (MQ=255)
                                                                                                                      ggTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAAc                                 >  2:7008/1‑150 (MQ=255)
                                                                                                                        tACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGCCTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCg                               >  1:1556965/1‑150 (MQ=255)
                                                                                                                        tACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCg                               >  1:63849/1‑150 (MQ=255)
                                                                                                                          cGGGCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACGGCAAATGGGTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGcc                             <  2:1045665/150‑1 (MQ=255)
                                                                                                                           ggTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCt                            <  1:395692/150‑1 (MQ=255)
                                                                                                                             tCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCa                          <  2:1528764/150‑1 (MQ=255)
                                                                                                                             gcTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAATCAACACTGCAAATGGTTGCTCCCGGTATCGCAGATGCGTTGATTGCGAATGCAATTGGTCTTTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCa                          <  1:441381/149‑1 (MQ=255)
                                                                                                                              cTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCaa                         >  1:552502/1‑150 (MQ=255)
                                                                                                                                gggggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAAcc                       >  2:476162/1‑150 (MQ=255)
                                                                                                                                gggggATCATGAACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAAcc                       >  2:1637655/1‑150 (MQ=255)
                                                                                                                                   ggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCATCACTGCAAATGGTTGCGCCCGGTATCTCAGAAGCGTTGATTGGGACTGCATTTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTTCAACAGCCTCAAACAgg                    >  1:321934/1‑149 (MQ=255)
                                                                                                                                   ggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAgc                    >  2:1716602/1‑150 (MQ=255)
                                                                                                                                   ggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAgc                    >  1:1215663/1‑150 (MQ=255)
                                                                                                                                       cATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGt                <  2:1178573/150‑1 (MQ=255)
                                                                                                                                          tgcttgttttACCGCCCCGAGGGCGATAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaa             >  1:660641/8‑150 (MQ=255)
                                                                                                                                          gCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaa             >  2:1031173/1‑150 (MQ=255)
                                                                                                                                           cACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCACCCAGCGCGTaaac            >  2:1536892/1‑150 (MQ=255)
                                                                                                                                           cACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaac            >  2:213228/1‑150 (MQ=255)
                                                                                                                                            cttgttttACCGCCCCGAGGGCGATGAAGCAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTa                                                        <  1:1533152/100‑1 (MQ=255)
                                                                                                                                            cttgttttACCGCCCCGAGGGCGATGAAGCAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTa                                                        >  2:1533152/6‑105 (MQ=255)
                                                                                                                                            aCGACTTTATAGCACTCGGGGCGGTAAAACAAGCACCACTGCATATGTTTGCGCCAGGTATCGCAGAAGCGTTGATTGAGACTGCAATTGGTCTGTTTGACGATATCACGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaaca           <  1:318848/150‑1 (MQ=255)
                                                                                                                                              gCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaacaaa         >  2:1261674/1‑150 (MQ=255)
                                                                                                                                               ccTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaacaaac        >  1:1137397/1‑150 (MQ=255)
                                                                                                                                               ccTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaacaaac        >  1:1722946/1‑150 (MQ=255)
                                                                                                                                                cTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTCTCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACt       <  1:430878/150‑1 (MQ=255)
                                                                                                                                                cTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACt       >  2:1039740/1‑150 (MQ=255)
                                                                                                                                                 tttATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTg      <  1:1004687/150‑1 (MQ=255)
                                                                                                                                                  ttATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTgg     >  1:1051857/1‑150 (MQ=255)
                                                                                                                                                  atATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCAGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTCTCCCGGCAGTTTAGGCCTACAACCGACTCATCCAGCGCGTAAACAAACTgg     >  1:1521621/2‑150 (MQ=255)
                                                                                                                                                   tATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGTCTGCAATTGGTCTGTTTGCCGCTATCACGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGa    >  1:1616712/1‑150 (MQ=255)
                                                                                                                                                     tCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAAc  >  1:1521608/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTGGAAGGGGCGTCGCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAAC  >  NC_000913/775464‑775762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: