Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 775,627 G→A 5.0% V159I (GTA→ATA)  tolQ → membrane spanning protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913775,6270GA 5.0% 318.2 / 3.2 99V159I (GTA→ATA) tolQmembrane spanning protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base G (48/46);  new base A (2/3);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 101 total aligned reads displayed.

GCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGACAA  >  NC_000913/775479‑775775
                                                                                                                                                    |                                                                                                                                                    
gcgTGCTATGCGTATCTCCATGAACCGGGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGt                                                                                                                                                     <  1:576897/150‑1 (MQ=255)
 ggTGCTATGCGTATCTGCATGAACCGTGATCTTGAAAATCTGGAAACGCAGATTCCGTTCCGCGGTACGGGTGGCTCCCTCACCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTa                                                                                                                                                    <  1:897335/149‑1 (MQ=255)
  ttGCTATGCGTATCTCCCTGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTaa                                                                                                                                                   <  1:1674887/149‑1 (MQ=255)
    gCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGGATATTGGTCTGGTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTaaaa                                                                                                                                                 >  1:1603733/1‑150 (MQ=255)
      tATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAACCGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACa                                                                                                                                               <  2:592572/150‑1 (MQ=255)
      tATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACa                                                                                                                                               <  2:546992/150‑1 (MQ=255)
       aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACaa                                                                                                                                              >  2:129354/1‑150 (MQ=255)
       aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTACCGCCCCGAGGGCGATaaaggcg                                                                                                                                              <  1:1410975/150‑5 (MQ=255)
       aTGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTCTGGTACGGTCTGGGGGATCATGCACGCCTTTACCGCCCCGAGGGCGATaaaggcg                                                                                                                                              <  1:1449618/150‑5 (MQ=255)
        tGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAg                                                                                                                                             <  1:195809/150‑1 (MQ=255)
        tGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAg                                                                                                                                             >  2:395692/1‑150 (MQ=255)
         gCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGc                                                                                                                                            <  2:1364818/150‑1 (MQ=255)
         gCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGc                                                                                                                                            <  2:1649605/150‑1 (MQ=255)
            tATCTCCATGATCCGTGATCTTGAAAATCTGGAAACGCACATTCCGGTCCTGGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGGTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAac                                                                                                                                         <  2:662946/150‑1 (MQ=255)
            tATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAac                                                                                                                                         <  1:1480412/150‑1 (MQ=255)
            tATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGGCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAac                                                                                                                                         <  2:1325437/150‑1 (MQ=255)
             aTCTCCATGAACCGTGAAATTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTCGGACGGGCTGGGGGATCATGCACGCCTTGATCGCCGTCGGGGGGGTAAGACAAggacca                                                                                                                                        >  1:289406/1‑145 (MQ=255)
                tcCATGAACCGTGAACTTGAAAATCTGGTAACGCACATTCCGGTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGGTTGCTACGGGCTGGGGGATCATGCACGCCTTTATCGCCCTAGGGGCGGTAAAACAATCATCACtc                                                                                                                                     >  1:250324/1‑149 (MQ=255)
                tcCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTg                                                                                                                                     >  1:319662/1‑150 (MQ=255)
                 ccATGAACCGTGAACTTGAAAATCTGGAAACGCACCTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACTGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGc                                                                                                                                    <  1:1542114/150‑1 (MQ=255)
                       aCCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATgg                                                                                                                              >  1:1528764/1‑150 (MQ=255)
                        ccGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGt                                                                                                                             >  1:991382/1‑150 (MQ=255)
                          gTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTg                                                                                                                           <  2:319662/150‑1 (MQ=255)
                          gTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTg                                                                                                                           <  2:1133357/150‑1 (MQ=255)
                             aaCTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTgcgc                                                                                                                        >  1:1038032/1‑150 (MQ=255)
                               cTTGAAAATCTGGAACCGCACATTCCGTTCCTAGGGACGGTTGGCTCCCTCAGCCCTTATATTGGTCTGTTTGGTACGGTATGGGGGATCGAGCACGACTTTTTCGCACGCGGGGCGGGAACACAAGCAACACGGCAAATGGTTgcgcac                                                                                                                      >  2:1141754/1‑148 (MQ=255)
                                ttGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCg                                                                                                                     <  1:1016380/150‑1 (MQ=255)
                                        cTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCa                                                                                                             <  1:259515/150‑1 (MQ=255)
                                              aCGCACATTCCGTTCCTCGGGACGGTTGGCTCCATCAGCCAGTATATTGGTCTGTTTGGTACGGGCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCg                                                                                                       <  1:1157967/150‑1 (MQ=255)
                                               cGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGt                                                                                                      >  1:1533550/1‑150 (MQ=255)
                                               cGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGt                                                                                                      >  2:664893/1‑150 (MQ=255)
                                                gCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGtt                                                                                                     <  2:425989/150‑1 (MQ=255)
                                                 cacaTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATAGTGGTCTGTTTGGTACTGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTg                                                                                                    <  2:1608953/150‑1 (MQ=255)
                                                  acaTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGa                                                                                                   >  1:871766/1‑150 (MQ=255)
                                                      tCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGc                                                                                               <  1:996879/150‑1 (MQ=255)
                                                        cGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTCTCGCAGAAGCGTTGATTGCgc                                                                                             >  2:1522027/1‑149 (MQ=255)
                                                         gTTCCTCGGTACGGGTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGAc                                                                                            <  1:689170/150‑1 (MQ=255)
                                                         gTTCCGCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGAc                                                                                            <  1:986649/150‑1 (MQ=255)
                                                           tcatCGGGACGGCTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATTCACTCCGTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTg                                                                                          <  1:1483803/147‑1 (MQ=255)
                                                            ccTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGc                                                                                         >  1:808403/1‑150 (MQ=255)
                                                            ccTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCGTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGc                                                                                         <  2:1717666/150‑1 (MQ=255)
                                                               cGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAAt                                                                                      >  1:1257754/1‑150 (MQ=255)
                                                                  tACGGGTGGCTCCCTCAGCCCGTATATTGGTCTGTTTGGTACGGTCGGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTgg                                                                                   <  1:692330/150‑1 (MQ=255)
                                                                        gggCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGtt                                                                             <  2:383141/149‑1 (MQ=255)
                                                                          gCTCCATCAGCCCGTTTTTTGGTGTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAATACTAGCAACTCTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTg                                                                           <  1:1588508/150‑1 (MQ=255)
                                                                            tCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATAGCCCTCGGGGCGGTAAAACAAGCACCACTGCAAATGGTTGCGCCCGGTATCGCAGCAGCGGTGAATGCGACTGCAATTGGTATGTTAGcc                                                                         >  1:135951/1‑150 (MQ=255)
                                                                            gccATCAGCCCGTATATTTGTCTGTTTGGTACGGTCGGGGGGATCATGCACGCCTTTATCGCCCGCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGcc                                                                         <  2:773452/149‑1 (MQ=255)
                                                                                    cccGTATATTGGTCTGGTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCgggatagc                                                                 <  1:918659/150‑8 (MQ=255)
                                                                                           atTGGTGTGTTTGGTACGGTCTGGGGGATCATGCACGCGTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGGTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGtt                                                          <  2:176707/150‑1 (MQ=255)
                                                                                           atTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGtt                                                          <  2:794491/150‑1 (MQ=255)
                                                                                             tGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTAt                                                        >  2:607175/1‑150 (MQ=255)
                                                                                              ggTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCATATGGTTGCGCCGGGTATCGCTGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTTTg                                                       >  1:1496876/1‑150 (MQ=255)
                                                                                               gtttGTTTGGTACGGGCTGGGGGAGCATGCACGCATTTCTCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATgg                                                      <  2:1083138/147‑1 (MQ=255)
                                                                                               gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGTAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATgg                                                      >  1:998523/1‑150 (MQ=255)
                                                                                               gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATgg                                                      <  2:371568/150‑1 (MQ=255)
                                                                                                 cTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCATATGGTTTAGCCCTGTATCGCAGAAGAGTTGATTGCGACTGAAATTGGTCTGTTTGCCGCTAGCCCGGCAGTTATGGcc                                                    >  1:1062549/1‑150 (MQ=255)
                                                                                                   gTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATGCCGGCAGTTATGGCCTa                                                  >  2:739627/1‑150 (MQ=255)
                                                                                                   gTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTa                                                  >  1:1067422/1‑150 (MQ=255)
                                                                                                   gTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTa                                                  >  2:739628/1‑150 (MQ=255)
                                                                                                   gTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTa                                                  >  2:1250849/1‑150 (MQ=255)
                                                                                                       ggTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAAc                                              >  2:7008/1‑150 (MQ=255)
                                                                                                         tACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGCCTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCg                                            >  1:1556965/1‑150 (MQ=255)
                                                                                                         tACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCg                                            >  1:63849/1‑150 (MQ=255)
                                                                                                           cGGGCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACGGCAAATGGGTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGcc                                          <  2:1045665/150‑1 (MQ=255)
                                                                                                            ggTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCt                                         <  1:395692/150‑1 (MQ=255)
                                                                                                              tCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCa                                       <  2:1528764/150‑1 (MQ=255)
                                                                                                              gcTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAATCAACACTGCAAATGGTTGCTCCCGGTATCGCAGATGCGTTGATTGCGAATGCAATTGGTCTTTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCa                                       <  1:441381/149‑1 (MQ=255)
                                                                                                               cTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCaa                                      >  1:552502/1‑150 (MQ=255)
                                                                                                                 gggggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAAcc                                    >  2:476162/1‑150 (MQ=255)
                                                                                                                 gggggATCATGAACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAAcc                                    >  2:1637655/1‑150 (MQ=255)
                                                                                                                    ggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCATCACTGCAAATGGTTGCGCCCGGTATCTCAGAAGCGTTGATTGGGACTGCATTTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTTCAACAGCCTCAAACAgg                                 >  1:321934/1‑149 (MQ=255)
                                                                                                                    ggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAgc                                 >  1:1215663/1‑150 (MQ=255)
                                                                                                                    ggATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAgc                                 >  2:1716602/1‑150 (MQ=255)
                                                                                                                        cATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGt                             <  2:1178573/150‑1 (MQ=255)
                                                                                                                           tgcttgttttACCGCCCCGAGGGCGATAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaa                          >  1:660641/8‑150 (MQ=255)
                                                                                                                           gCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaa                          >  2:1031173/1‑150 (MQ=255)
                                                                                                                            cACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCACCCAGCGCGTaaac                         >  2:1536892/1‑150 (MQ=255)
                                                                                                                            cACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaac                         >  2:213228/1‑150 (MQ=255)
                                                                                                                             cttgttttACCGCCCCGAGGGCGATGAAGCAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTa                                                                     >  2:1533152/6‑105 (MQ=255)
                                                                                                                             cttgttttACCGCCCCGAGGGCGATGAAGCAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTa                                                                     <  1:1533152/100‑1 (MQ=255)
                                                                                                                             aCGACTTTATAGCACTCGGGGCGGTAAAACAAGCACCACTGCATATGTTTGCGCCAGGTATCGCAGAAGCGTTGATTGAGACTGCAATTGGTCTGTTTGACGATATCACGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaaca                        <  1:318848/150‑1 (MQ=255)
                                                                                                                               gCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaacaaa                      >  2:1261674/1‑150 (MQ=255)
                                                                                                                                ccTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaacaaac                     >  1:1137397/1‑150 (MQ=255)
                                                                                                                                ccTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTaaacaaac                     >  1:1722946/1‑150 (MQ=255)
                                                                                                                                 cTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTCTCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACt                    <  1:430878/150‑1 (MQ=255)
                                                                                                                                 cTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACt                    >  2:1039740/1‑150 (MQ=255)
                                                                                                                                  tttATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTg                   <  1:1004687/150‑1 (MQ=255)
                                                                                                                                   ttATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTgg                  >  1:1051857/1‑150 (MQ=255)
                                                                                                                                   atATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCAGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTCTCCCGGCAGTTTAGGCCTACAACCGACTCATCCAGCGCGTAAACAAACTgg                  >  1:1521621/2‑150 (MQ=255)
                                                                                                                                    tATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGTCTGCAATTGGTCTGTTTGCCGCTATCACGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGa                 >  1:1616712/1‑150 (MQ=255)
                                                                                                                                      tCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAAc               >  1:1521608/1‑150 (MQ=255)
                                                                                                                                       cGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACt              <  2:1611968/150‑1 (MQ=255)
                                                                                                                                        gCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGACGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTg             >  2:848279/1‑150 (MQ=255)
                                                                                                                                         cccTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGa            <  2:552502/150‑1 (MQ=255)
                                                                                                                                             cGGGGCGGTAAAACACGCAACCCTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTCCTATTGGTCTGATTGCCCCTATCCCGGCAGTTATGGCCTACAACCGCGGCGACCAGCGCGTCAACAAACTGGAACGGaagtc        >  2:886865/1‑147 (MQ=255)
                                                                                                                                              ggggCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTAc       <  1:7008/150‑1 (MQ=255)
                                                                                                                                                 gCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGAc    >  2:553577/1‑150 (MQ=255)
                                                                                                                                                 gCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGAc    <  1:60691/150‑1 (MQ=255)
                                                                                                                                                   ggTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGACaa  >  1:1339337/1‑150 (MQ=255)
                                                                                                                                                   ggTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGACaa  >  1:134037/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCAGCGCGTAAACAAACTGGAACTGAATTACGACAA  >  NC_000913/775479‑775775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: