Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 824,573 T→A 5.2% intergenic (‑76/‑57) ybhP ← / → ybhQ endo/exonuclease/phosphatase family protein/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913824,5730TA 5.2% 316.6 / 3.5 106intergenic (‑76/‑57)ybhP/ybhQendo/exonuclease/phosphatase family protein/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (52/48);  new base A (3/3);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TTAAACGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGGTGGG  >  NC_000913/824424‑824721
                                                                                                                                                     |                                                                                                                                                    
ttAAAAGAGGTAAAGCATTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTAGTGTTTGATCGGGCATAATTATCCTGTCTTTGCCTCTTATCTCAGTGAAATAGTGAAGTCTGCGTCACAAAAATGTGCGGTCTTACGGAAtt                                                                                                                                                      <  1:266298/150‑1 (MQ=255)
  aaaCGAGGTAAACCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATCGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAAtttt                                                                                                                                                    <  2:1024481/150‑1 (MQ=255)
   aaCGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTc                                                                                                                                                   >  2:1493614/1‑150 (MQ=255)
   aaCGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTc                                                                                                                                                   <  2:1113815/150‑1 (MQ=255)
   aaCGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTc                                                                                                                                                   >  2:635519/1‑150 (MQ=255)
      gcgGTAAAGCCTTTGTGAATATTGATGGTGATCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCAGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGt                                                                                                                                                <  2:1454897/150‑1 (MQ=255)
      gcgGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGt                                                                                                                                                <  1:185425/150‑1 (MQ=255)
      gcgGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCAGCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGt                                                                                                                                                <  2:1448167/150‑1 (MQ=255)
       cgGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGTCGTCACAAAAAGGTGCGGTCTTACGGAAAATTCCGTa                                                                                                                                               <  2:920735/150‑1 (MQ=255)
             aGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTc                                                                                                                                         >  1:1325349/1‑150 (MQ=255)
              gCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCGTGTCTTTGCCTCTTATCTCATTGATATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTTCGGAATTTTCCGTAAAGTTCg                                                                                                                                        >  2:910543/1‑150 (MQ=255)
              gCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCg                                                                                                                                        <  1:229551/150‑1 (MQ=255)
               ccTTTGTGAATATTGGTGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCgg                                                                                                                                       <  2:1067725/150‑1 (MQ=255)
               ccTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGGCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCgg                                                                                                                                       <  1:1216566/150‑1 (MQ=255)
               ccTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCATCTCTTTGCCTCTTATCTCATTGAAATAGAGAAGTCGTCGTCACAAAAAGGTGCGGTCTTAACGAAATTTCCGTAAAGTTCgg                                                                                                                                       >  2:1696125/1‑150 (MQ=255)
                cTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGt                                                                                                                                      <  2:853597/150‑1 (MQ=255)
                    gtgAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActc                                                                                                                                  <  2:572237/150‑1 (MQ=255)
                    gtgAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGACTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTAAGTAAAGTTCTGTActc                                                                                                                                  >  2:765354/1‑150 (MQ=255)
                     tgAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTActct                                                                                                                                 >  1:1559910/1‑150 (MQ=255)
                      gAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTg                                                                                                                                <  1:1007014/150‑1 (MQ=255)
                       aaTATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGa                                                                                                                               >  2:1573531/1‑150 (MQ=255)
                         tatTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagt                                                                                                                             <  2:538842/150‑1 (MQ=255)
                         taatGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagt                                                                                                                             <  1:6369/147‑1 (MQ=255)
                             gATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAACGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaagt                                                                                                                         <  2:1367711/150‑1 (MQ=255)
                             gATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGagtaagt                                                                                                                         <  2:1374386/150‑1 (MQ=255)
                                    aGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATa                                                                                                                  >  1:1584202/1‑150 (MQ=255)
                                        ccTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTATAGATAAAtt                                                                                                              >  2:124271/1‑150 (MQ=255)
                                        ccTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAtt                                                                                                              >  2:1608734/1‑150 (MQ=255)
                                        ccTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAtt                                                                                                              >  2:854513/1‑150 (MQ=255)
                                          ttGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAAttct                                                                                                            <  2:297684/150‑1 (MQ=255)
                                               cGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAgg                                                                                                       >  2:362882/1‑150 (MQ=255)
                                               cGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAgg                                                                                                       >  2:298773/1‑150 (MQ=255)
                                                 aaaaTTGAGGGGTTGCATCGGGCATAATTATCCAGTCTTTGCCACTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGgag                                                                                                     <  2:318566/150‑1 (MQ=255)
                                                       gttgtGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGc                                                                                               >  1:189884/1‑150 (MQ=255)
                                                            gtTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGa                                                                                          >  1:1428167/1‑150 (MQ=255)
                                                               tGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGt                                                                                       <  1:891122/150‑1 (MQ=255)
                                                                gATCGGTAATAATTTTCCTGTCTTTGCATCTTATCTCATGGAAATAGTGTTGTCTTCGTCACAAAAAGGTGCGGTCTTACGTAATTTTCCGTAAAGTTAGGTACTCTGAGTAATGTGAGATATATTCTTCAGTAGAGAAGACATTAAGTg                                                                                      >  2:305975/1‑150 (MQ=255)
                                                                 aTCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTgg                                                                                     <  1:635519/150‑1 (MQ=255)
                                                                  tCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGTAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGc                                                                                    <  2:1369456/150‑1 (MQ=255)
                                                                   cGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGGCGGCGTCACAAAACGGTGCGGGCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGCGAAGCCATGAAGTGGca                                                                                   <  2:343044/150‑1 (MQ=255)
                                                                        aTAATTTTCATTTCTTTGCCTCTTATCTCTTTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCGTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCaacaac                                                                              <  1:908984/150‑1 (MQ=255)
                                                                        aTAAATTTCCTGTCTTTGCCTCTTAACTCATTGAAATAGTCTAGGCGGCGGCACAAACAGGGGCGGTCTTACGGAATTTTCCGTAAAGTGCGGTACTCTGAGTAAGTAGAGATAAAATCTTCAGGAGAGAAGCCATGAAGTGGCaacaac                                                                              <  1:915069/150‑1 (MQ=255)
                                                                         tAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGAGGTCTTACGGAATTTTACGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCTGGAGAGAAGCCATGAAGTGGCaactact                                                                             >  1:262128/1‑149 (MQ=255)
                                                                            ttttCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACgtgt                                                                          >  1:682088/1‑150 (MQ=255)
                                                                              ttCCTGTCTTTGCCTCTTATCTCATTGAAATAGTTTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTc                                                                        <  2:1400796/150‑1 (MQ=255)
                                                                                ccTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCgt                                                                      <  1:894616/150‑1 (MQ=255)
                                                                                 cTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCgtg                                                                     >  1:1732139/1‑150 (MQ=255)
                                                                                   gTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTc                                                                   <  1:29955/150‑1 (MQ=255)
                                                                                       ttGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATATTCCGTAAAGATCGGTACTCTGAGTAAGAAGAGATAAATTCTTCAGGAGAGAATCCATGAAGAGGCAACAACGTGTTCGTGTCGCaa                                                               >  2:530319/1‑150 (MQ=255)
                                                                                        tGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTATGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAAc                                                              >  2:1527512/1‑150 (MQ=255)
                                                                                        tGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCATCAACGTCTTCGTGTCGCAAc                                                              >  2:895015/1‑150 (MQ=255)
                                                                                         gCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGATTTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACg                                                             >  1:155499/1‑150 (MQ=255)
                                                                                         gCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACg                                                             <  1:975810/150‑1 (MQ=255)
                                                                                          ccTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACgg                                                            >  2:460308/1‑150 (MQ=255)
                                                                                           ctctTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACggg                                                           >  1:1564375/1‑150 (MQ=255)
                                                                                             ctTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTGAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTGGTGTCGCAACggggc                                                         >  2:1163200/1‑148 (MQ=255)
                                                                                              ttATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCt                                                        <  2:378553/150‑1 (MQ=255)
                                                                                              ttATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCt                                                        <  2:1413782/150‑1 (MQ=255)
                                                                                              ttATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCt                                                        <  2:756109/150‑1 (MQ=255)
                                                                                              tattatcATTGAAATATTGTAGTCTGCGTAACAAATAGGTGAGTTCATACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCAATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCt                                                        <  2:1293754/145‑1 (MQ=255)
                                                                                               tATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTa                                                       <  2:189884/150‑1 (MQ=255)
                                                                                                  ctcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGt                                                    >  1:1400413/1‑150 (MQ=255)
                                                                                                  ctcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGt                                                    >  1:1561024/1‑150 (MQ=255)
                                                                                                   tcATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGtt                                                   >  1:913115/1‑150 (MQ=255)
                                                                                                    cATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTg                                                  >  1:672045/1‑150 (MQ=255)
                                                                                                        gAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGCTAAATACTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGGCTAAGTTGCTgg                                              >  2:335070/1‑150 (MQ=255)
                                                                                                        gAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTgg                                              >  2:1578127/1‑150 (MQ=255)
                                                                                                          aaTAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTTTTCGTGTCGCAACTGGTCTAAGTTGCTGGCa                                            >  2:121987/1‑150 (MQ=255)
                                                                                                           aTAGTGTAGGCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCTTAAAGTGCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACCTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAg                                           <  1:868408/150‑1 (MQ=255)
                                                                                                            tAGTGTAGTCGGCGTCTCAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGa                                          >  2:466588/1‑150 (MQ=255)
                                                                                                                gtAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTAt                                      >  1:233186/1‑150 (MQ=255)
                                                                                                                  aGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCGAAGTTGCTGGCAGATTAtat                                    >  1:48180/1‑148 (MQ=255)
                                                                                                                   gTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGtt                                   <  1:539360/150‑1 (MQ=255)
                                                                                                                   gTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGtt                                   <  1:1013524/150‑1 (MQ=255)
                                                                                                                    tCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTg                                  <  2:193790/150‑1 (MQ=255)
                                                                                                                         gTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCTGATTATGTTGCAttt                             >  1:198502/1‑150 (MQ=255)
                                                                                                                            acaAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACt                          <  1:1573531/150‑1 (MQ=255)
                                                                                                                             caAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTg                         <  2:867141/150‑1 (MQ=255)
                                                                                                                              aaaaaGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACGCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTgg                        <  2:327591/150‑1 (MQ=255)
                                                                                                                               aaaaGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGTTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGt                       >  2:1125417/1‑150 (MQ=255)
                                                                                                                               aaaaGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGt                       >  2:1371855/1‑150 (MQ=255)
                                                                                                                                  aGGAGCGGTCTAACGGAATTTTCCGGAAAGTTCGTTACTCTGAGTAAGTTGAGATAAATTCTTAAGGAGAGAAGCCATGAAGTGGCAACAACGTGTGCGTGTCGCAACGGGGCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGt                    <  2:244065/150‑1 (MQ=255)
                                                                                                                                   ggTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTg                   >  2:1008181/1‑150 (MQ=255)
                                                                                                                                      gCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGgcg                <  1:362882/150‑1 (MQ=255)
                                                                                                                                       gaactttACGGAAAATTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGgcgc               >  1:1362751/6‑150 (MQ=255)
                                                                                                                                          ctttACGGAAAATTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCgctgc            >  1:434344/3‑150 (MQ=255)
                                                                                                                                            ttACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGTTAGTGGCGctgctg          >  1:1291335/1‑150 (MQ=255)
                                                                                                                                              aCGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCACCGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggt        <  1:102856/150‑1 (MQ=255)
                                                                                                                                              aCGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTTGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggt        >  2:1148836/1‑150 (MQ=255)
                                                                                                                                              aCGGAATTTTCAGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggt        >  2:1689278/1‑150 (MQ=255)
                                                                                                                                               cGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggtg       >  1:1370485/1‑150 (MQ=255)
                                                                                                                                               cGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGCTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggtg       >  1:1267376/1‑150 (MQ=255)
                                                                                                                                               cGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTCGTCGTGTCGCAACGGGACTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTAGCGCTGCtggtg       >  1:1284339/1‑150 (MQ=255)
                                                                                                                                                ggAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggtgg      <  2:1559910/150‑1 (MQ=255)
                                                                                                                                                  aaTTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggtggtg    <  1:453353/150‑1 (MQ=255)
                                                                                                                                                   aTTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggtggtgg   >  1:1676763/1‑150 (MQ=255)
                                                                                                                                                   aTTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCtggtggtgg   <  2:1194410/150‑1 (MQ=255)
                                                                                                                                                    ttttCCGTATAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGGTggg  <  2:804339/150‑1 (MQ=255)
                                                                                                                                                    ttttCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGGTggg  <  2:1561024/150‑1 (MQ=255)
                                                                                                                                                    ttttCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGGTggg  <  2:1400413/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTAAACGCGGTAAAGCCTTTGTGAATATTGATGGTGAGCACCTTGAACGAAAATTGTTGTGTTTGATCGGGCATAATTTTCCTGTCTTTGCCTCTTATCTCATTGAAATAGTGTAGTCGGCGTCACAAAAAGGTGCGGTCTTACGGAATTTTCCGTAAAGTTCGGTACTCTGAGTAAGTAGAGATAAATTCTTCAGGAGAGAAGCCATGAAGTGGCAACAACGTGTTCGTGTCGCAACGGGTCTAAGTTGCTGGCAGATTATGTTGCATTTACTGGTAGTGGCGCTGCTGGTGGTGGG  >  NC_000913/824424‑824721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: