Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 832,365 A→C 9.8% intergenic (+129/+103) rhlE → / ← ybiA ATP‑dependent RNA helicase/DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913832,3650AC 9.8% 236.0 / 11.1 86intergenic (+129/+103)rhlE/ybiAATP‑dependent RNA helicase/DUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base A (35/42);  new base C (3/6);  total (38/48)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGC  >  NC_000913/832216‑832514
                                                                                                                                                     |                                                                                                                                                     
aaaCCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTACATGCTTTTGTAGGCCGGATaa                                                                                                                                                       >  2:737648/1‑150 (MQ=255)
 aaCCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAg                                                                                                                                                      >  1:319333/1‑150 (MQ=255)
  aCCTGCCTCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAgg                                                                                                                                                     <  1:1172160/150‑1 (MQ=255)
   ccTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGc                                                                                                                                                    <  2:377528/150‑1 (MQ=255)
    cTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCg                                                                                                                                                   <  1:1223828/150‑1 (MQ=255)
     tGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATaacgttg                                                                                                                                                  >  1:406006/1‑145 (MQ=255)
      gCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGta                                                                                                                                                 <  2:1398205/150‑2 (MQ=255)
         gCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAc                                                                                                                                              <  2:1655802/150‑1 (MQ=255)
          cTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcg                                                                                                                                             <  2:1074225/150‑1 (MQ=255)
            gcgcAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                           >  1:1370479/1‑150 (MQ=255)
             cgcAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          <  2:1089521/150‑1 (MQ=255)
              gcAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAAcgcctt                                                                                                                                         <  2:1230729/150‑3 (MQ=255)
                  tAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                     <  2:1393416/150‑1 (MQ=25)
                  tAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                     >  1:1311530/1‑150 (MQ=255)
                  tAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATcc                                                                                                                                     >  1:1318570/1‑150 (MQ=255)
                     tCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                  <  1:499799/150‑1 (MQ=21)
                     tCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                  <  2:1513485/150‑1 (MQ=255)
                     tCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                  <  2:545926/150‑1 (MQ=255)
                      tttttATTCCTGGCGAAGCCCGGCATCAGGCTGATGAACACACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCGTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                 <  2:1085373/149‑2 (MQ=18)
                      cTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                 <  1:1255782/150‑2 (MQ=21)
                            tGCCGGGCTATGCCCGGCATCAGGCTGATGAACAACCGCAAACCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcac                                                                                                                           <  1:1090112/150‑1 (MQ=255)
                             gCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcaca                                                                                                                          <  1:1213568/150‑1 (MQ=255)
                               cGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCacaac                                                                                                                        <  1:670760/150‑1 (MQ=255)
                               cGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGAATTTCacaac                                                                                                                        >  2:357089/1‑150 (MQ=255)
                                 ggCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCAcaacaa                                                                                                                      >  1:951690/1‑150 (MQ=255)
                                    tATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCa                                                                                                                   >  1:490710/1‑150 (MQ=255)
                                      tGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACt                                                                                                                 <  1:499544/150‑1 (MQ=255)
                                          cGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTc                                                                                                             <  2:115192/150‑1 (MQ=255)
                                           ggCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCa                                                                                                            >  2:677480/1‑150 (MQ=255)
                                            gCATCAGGCTGATGAACAACCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAg                                                                                                           <  1:450916/150‑1 (MQ=255)
                                            gCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAg                                                                                                           <  2:381985/150‑1 (MQ=255)
                                            gCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAg                                                                                                           <  2:153097/150‑1 (MQ=255)
                                             cATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGc                                                                                                          >  2:1384156/1‑150 (MQ=255)
                                                   gCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTg                                                                                                    <  2:63862/150‑1 (MQ=255)
                                                   gCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTg                                                                                                    <  1:171025/150‑1 (MQ=255)
                                                        tgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAac                                                                                               <  1:483825/150‑1 (MQ=255)
                                                            cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACgg                                                                                           <  1:1551481/150‑1 (MQ=255)
                                                            cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTGTTGTAGGCCGGATACGGCGTTTCCGCCGCATCCGGCATTTCACAAAAAGCCCTAGTCAGCAATTTgagcaaccgg                                                                                           >  1:847531/1‑143 (MQ=18)
                                                             aaaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACggg                                                                                          <  2:319333/150‑1 (MQ=255)
                                                              aaCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGa                                                                                         >  1:409134/1‑150 (MQ=255)
                                                                cGCAAAACTGCCTGATGCGCTACGCTTATCCGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTCGGCCGGATACGGAGTATACGCCGCATCCGGCATTTCACAACAAGCACTAGTCAGAAATGTGAGAACACGGGaaa                                                                                       >  1:273376/1‑150 (MQ=21)
                                                                cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGaaa                                                                                       >  2:1243878/1‑150 (MQ=255)
                                                                    aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTACATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCGATTTGAGAACACGGGAAAAAAt                                                                                   <  1:737648/150‑1 (MQ=255)
                                                                        tGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTAt                                                                               >  2:517849/1‑150 (MQ=255)
                                                                         gCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATc                                                                              <  1:556870/150‑1 (MQ=255)
                                                                         gCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGCGAACACGGGAAAAAATTTATc                                                                              <  1:946556/150‑1 (MQ=255)
                                                                         gCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATc                                                                              <  2:1674276/150‑1 (MQ=255)
                                                                          ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCt                                                                             >  1:1544261/1‑150 (MQ=255)
                                                                                 cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAc                                                                      >  1:837809/1‑150 (MQ=255)
                                                                                  gcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAcc                                                                     >  1:943650/1‑150 (MQ=255)
                                                                                  gcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTAcc                                                                     >  2:603676/1‑150 (MQ=255)
                                                                                   cTACGCTTATCAGGCCTACGTGATCTCTGCAATATATTGAATTTGCAGGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTTAGAACACGGGAAAAAATTTATCTGATTTACCg                                                                    >  1:53865/1‑150 (MQ=255)
                                                                                   cTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCg                                                                    <  1:1279800/150‑1 (MQ=255)
                                                                                   cTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGAATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTATAccc                                                                    >  1:77951/1‑149 (MQ=255)
                                                                                   cTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGAATCCGGAATTTCACAACAAGCACTTGTCAGCAATTTTAGAACACGGGAAAAAATTTATCTGTTTTAcac                                                                    >  2:886993/1‑148 (MQ=255)
                                                                                     aCGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGcc                                                                  >  1:438628/1‑150 (MQ=255)
                                                                                           atgaGGCCTACGTGAACTCTGCAATATATTGAATTTTCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAg                                                            <  1:261105/147‑1 (MQ=255)
                                                                                           aTCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAg                                                            <  2:1715319/150‑1 (MQ=255)
                                                                                              aGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCa                                                         >  2:1649532/1‑150 (MQ=255)
                                                                                              aGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTATACCCCCCATaatcaa                                                         >  2:852647/1‑146 (MQ=255)
                                                                                                   tACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCgag                                                    <  2:195353/150‑1 (MQ=255)
                                                                                                   tACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCgag                                                    <  2:748826/150‑1 (MQ=255)
                                                                                                          aCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTAc                                             <  1:730866/150‑1 (MQ=255)
                                                                                                           ctctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACt                                            >  2:617201/1‑150 (MQ=255)
                                                                                                           ctctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTGGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACt                                            <  1:1555777/150‑1 (MQ=255)
                                                                                                            tctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACTACAATCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACtt                                           <  2:1530810/150‑1 (MQ=255)
                                                                                                             ctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTtc                                          >  1:124501/1‑150 (MQ=255)
                                                                                                              tGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTtct                                         <  2:146778/150‑1 (MQ=255)
                                                                                                                   tatatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCtata                                    <  2:438628/150‑1 (MQ=255)
                                                                                                                      atTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTtc                                          <  2:606697/141‑1 (MQ=255)
                                                                                                                      atTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTtc                                          >  1:606697/1‑141 (MQ=255)
                                                                                                                      atTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGcc                                 >  2:972349/1‑150 (MQ=255)
                                                                                                                      atTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGcc                                 >  2:845903/1‑150 (MQ=255)
                                                                                                                        tGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGCGACGTTACTTCTCtaggatcgg                               >  1:1058179/1‑143 (MQ=255)
                                                                                                                         gAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCTATTTGAGAACACGGGAAAAAATTTATCAGTTTTACCGCCCATAATCAACCGAGACTTTACAGCTCTATACCCact                              >  1:330158/1‑148 (MQ=37)
                                                                                                                           aTTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTg                            <  1:224833/150‑1 (MQ=255)
                                                                                                                              tGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGCCGTTACTTCTCTATAGCCAATTGTTc                         >  2:975654/1‑150 (MQ=255)
                                                                                                                                cATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTcgc                       <  2:1521784/150‑1 (MQ=255)
                                                                                                                                     ttttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAAc                  <  2:951690/150‑1 (MQ=255)
                                                                                                                                       ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTc                >  2:1432899/1‑150 (MQ=255)
                                                                                                                                        tGTAGGCCGGATAAGGCGTTTACGCCGCGTCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACAGAGACGTAACTTCTCTATAGCCATTTGTTCGCGCAACTcc               >  2:303849/1‑150 (MQ=255)
                                                                                                                                           atgCCGGATGCGGCGTAAACGCCTTATCCGGCATTTCACAACAAGCACTTGTCAGCAATGTGAGAACACGGGAAAAAATTTTTCTGTTTTACCGCCCATCAGCCACCGAGACGTTACTTCTCTTTAGCCAATTGTTCGCGCAACTCCAtt            >  1:1304251/3‑150 (MQ=255)
                                                                                                                                           aGGACGGATAAGGCGTTTAAGACGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCAtt            <  2:1228304/150‑1 (MQ=255)
                                                                                                                                            ggCCGTATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTa           <  2:164046/150‑1 (MQ=255)
                                                                                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTa           >  2:1013215/1‑150 (MQ=255)
                                                                                                                                            ggCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTa           >  2:1187251/1‑150 (MQ=255)
                                                                                                                                              ccGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTaaa         >  1:87754/1‑150 (MQ=255)
                                                                                                                                               cGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTaaaa        <  2:837809/150‑1 (MQ=255)
                                                                                                                                                     aGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGc  >  2:513211/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACCTGCCGCTGCGCAGTAATCTTTTATGCCGGGCTATGCCCGGCATCAGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGTCAGCAATTTGAGAACACGGGAAAAAATTTATCTGTTTTACCGCCCATAAGCCACCGAGACGTTACTTCTCTATAGCCAATTGTTCGCGCAACTCCATTAAAAGGTAGC  >  NC_000913/832216‑832514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: