Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 845,575 G→A 5.6% intergenic (‑95/+166) ybiO ← / ← glnQ mechanosensitive channel protein, intermediate conductance/glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913845,5750GA 5.6% 391.1 / 6.7 123intergenic (‑95/+166)ybiO/glnQmechanosensitive channel protein, intermediate conductance/glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (64/52);  new base A (5/2);  total (69/54)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 128 total aligned reads displayed.

CCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCA  >  NC_000913/845426‑845723
                                                                                                                                                     |                                                                                                                                                    
 cgcgTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCgg                                                                                                                                                     >  2:1286411/1‑150 (MQ=255)
 cgcgTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCgg                                                                                                                                                     >  1:1123255/1‑150 (MQ=255)
 cgcgTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCgg                                                                                                                                                     >  2:1223714/1‑150 (MQ=255)
   cgTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGTCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGt                                                                                                                                                   <  1:58212/150‑1 (MQ=255)
   cgTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGt                                                                                                                                                   >  2:1029358/1‑150 (MQ=255)
      gggCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGaa                                                                                                                                                >  1:133191/1‑150 (MQ=255)
        gCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAg                                                                                                                                              <  2:1062284/150‑1 (MQ=255)
         cAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCAGGACTCCTCCAGTGAGAAAATCGAGCAAAAAGCTAAGTAAAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCGGGTGTGCCTCCCTTTCGGGTGAAAgg                                                                                                                                             <  1:739432/150‑1 (MQ=255)
           ggTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGgga                                                                                                                                           >  2:392635/1‑150 (MQ=255)
             ggcgcCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCACCCGAAAGggagg                                                                                                                                         <  1:664471/149‑1 (MQ=255)
              gcgcCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCACCCGAAAGggaggc                                                                                                                                        <  2:250338/150‑2 (MQ=255)
               cgcCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGaa                                                                                                                                       >  2:674046/1‑150 (MQ=255)
                 cccAGCAGGCAGAAGAGGATGAACAGGCTCCACCGCATGACTCCGCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCGGGAGGAGGGGCGATTGTAAAAGAGAGGAATCTGGTGTGCCTCACTGTCGGGTGAAAGGtaggtagg                                                                                                                                     >  2:858821/1‑150 (MQ=255)
                 cccAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACACCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTATTTGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAgg                                                                                                                                     <  1:528547/150‑1 (MQ=255)
                  ccAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAggg                                                                                                                                    >  1:187763/1‑150 (MQ=255)
                  ccAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAggg                                                                                                                                    >  2:1686822/1‑150 (MQ=255)
                    agcagGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGat                                                                                                                                  <  2:1585976/150‑1 (MQ=255)
                       aggcagAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATAtt                                                                                                                               <  1:1189384/150‑1 (MQ=255)
                                cggatccaCAGGATCCACCTCATGACTGCTCCAGTGAGAAAATAGAGCATAAAGATAAGTGTAGAGGCTGGTGGAGGGGCGATTTTAACTGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTTAGCAGGtt                                                                                                                      <  1:155134/143‑1 (MQ=255)
                                 ggATGAACAGGATCCACCGCATGACTGCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTg                                                                                                                     >  2:1347590/1‑150 (MQ=255)
                                 ggATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTg                                                                                                                     >  2:735457/1‑150 (MQ=255)
                                 ggATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTg                                                                                                                     >  2:1385024/1‑150 (MQ=255)
                                    tGAACATCATCCACCCCATTACACCTCCAGTGAGAAAATAGATGAAATAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTg                                                                                                                  <  1:141074/150‑1 (MQ=255)
                                    tGAACAGGATCCACCGCATGACTCCTACAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCGGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTg                                                                                                                  <  2:1209580/150‑1 (MQ=255)
                                     gAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTATGAAGGTtgatga                                                                                                                 >  2:1451385/1‑150 (MQ=255)
                                     gAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGa                                                                                                                 >  2:544955/1‑150 (MQ=255)
                                      aaCAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGAc                                                                                                                >  2:571530/1‑150 (MQ=255)
                                       aCAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATAATAATCAGGATGCTGAcc                                                                                                               >  1:1577454/1‑149 (MQ=255)
                                         aGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAAc                                                                                                             >  2:1182758/1‑150 (MQ=255)
                                          ggATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACg                                                                                                            >  1:203772/1‑150 (MQ=255)
                                          ggATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACg                                                                                                            >  1:1609168/1‑150 (MQ=255)
                                              ccACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCg                                                                                                        <  1:142225/150‑1 (MQ=255)
                                               cACCGCATGACTCCTCCAGTGAGAAAATATAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGTATATTAACCAGGTTGCTGAAAACGTGCgc                                                                                                       >  1:257657/1‑149 (MQ=255)
                                                 ccGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGc                                                                                                     >  2:714667/1‑150 (MQ=255)
                                                  cGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACACCGTGCGGGCt                                                                                                    >  2:87766/1‑150 (MQ=255)
                                                   gCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtt                                                                                                   >  2:402996/1‑150 (MQ=255)
                                                       gACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGTAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtttttt                                                                                               >  2:881395/1‑150 (MQ=255)
                                                       gACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGACGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtttttt                                                                                               >  2:1099569/1‑150 (MQ=255)
                                                         ctcctcCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGc                                                                                             >  2:1157156/1‑150 (MQ=255)
                                                           cctccAGTGAGAAAATAGAGCATAATGATTAGTTTAGATGCTGGAGGAGGGGCGATTTTTAATGAGAGGACTCTGGTGTGCCTCCCTTTCGGGTGATTGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGcgg                                                                                           <  2:1160217/150‑3 (MQ=255)
                                                            ctccAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCgg                                                                                          <  1:1686822/150‑1 (MQ=255)
                                                                  tGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgcgg                                                                                    >  2:1029559/1‑150 (MQ=255)
                                                                    agaAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgcggcg                                                                                  <  2:797903/150‑1 (MQ=255)
                                                                    agaAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgcggcg                                                                                  >  1:177391/1‑150 (MQ=255)
                                                                    agaAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGAGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgcggcg                                                                                  <  2:444017/150‑1 (MQ=255)
                                                                      aaaaTAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTg                                                                                <  1:1448964/150‑1 (MQ=255)
                                                                          tAGCGCAAAAAGATAAGTATAGTTGCTGGATGAGGGGCGATTTTAAATGAGAGGAAGCGGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACg                                                                            <  2:200255/150‑1 (MQ=255)
                                                                           agagCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGc                                                                           >  1:1197407/1‑150 (MQ=255)
                                                                            gagCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGcc                                                                          <  2:1188398/150‑1 (MQ=255)
                                                                              gCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCtt                                                                        >  2:313504/1‑150 (MQ=255)
                                                                                aaaaaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTTAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTAt                                                                      <  2:1314370/150‑1 (MQ=255)
                                                                                aaaaaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTAt                                                                      >  2:659088/1‑150 (MQ=255)
                                                                                  aaaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATcc                                                                    <  2:524355/150‑1 (MQ=255)
                                                                                   aaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCg                                                                   <  1:392635/150‑1 (MQ=255)
                                                                                     gATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGATAGGGAGGAAGGGATATTACGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTc                                                                 <  1:894282/150‑1 (MQ=255)
                                                                                     gATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTc                                                                 <  2:1123255/150‑1 (MQ=255)
                                                                                      aTAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                <  2:203772/150‑1 (MQ=255)
                                                                                      aTAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                <  2:1162812/150‑1 (MQ=255)
                                                                                       tAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCt                                                               <  2:1677564/150‑1 (MQ=255)
                                                                                       tAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCt                                                               >  2:1614222/1‑150 (MQ=255)
                                                                                        aaGTATAGATGCTGGAGTAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTa                                                              <  1:1458048/150‑1 (MQ=255)
                                                                                          gTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCGTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACa                                                            <  1:139603/150‑1 (MQ=255)
                                                                                           tataGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                           >  1:774040/1‑150 (MQ=255)
                                                                                           tataGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCATCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGTCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                           >  1:70221/1‑150 (MQ=255)
                                                                                              aGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGtt                                                        >  1:945349/1‑150 (MQ=255)
                                                                                               gATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTc                                                       >  1:567755/1‑150 (MQ=255)
                                                                                                 tGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCAt                                                     >  1:139122/1‑150 (MQ=255)
                                                                                                  gCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATg                                                    <  1:1728269/150‑1 (MQ=255)
                                                                                                  gCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATg                                                    <  1:1576250/150‑1 (MQ=255)
                                                                                                     ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCaa                                                 >  2:1671699/1‑150 (MQ=255)
                                                                                                       aggaggGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAAtt                                               >  2:1095340/1‑150 (MQ=255)
                                                                                                         gaggGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAAtttt                                             <  1:1029358/150‑1 (MQ=255)
                                                                                                             ggCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTtaata                                         >  1:1213676/1‑150 (MQ=255)
                                                                                                             ggCGATTTTAAATGAGAGGAATCGGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTtaata                                         <  1:968730/150‑1 (MQ=255)
                                                                                                                gATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGTGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCTTCCTACAAGTTCATGCAATTTTAATAAGt                                      >  1:674341/1‑150 (MQ=255)
                                                                                                                gATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGt                                      <  1:1223714/150‑1 (MQ=255)
                                                                                                                  ttttAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTg                                    >  1:869220/1‑150 (MQ=255)
                                                                                                                       aaTGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgc                                                                                      >  1:827815/1‑95 (MQ=255)
                                                                                                                       aaTGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGa                               >  2:1517956/1‑150 (MQ=255)
                                                                                                                       aaTGAGAGGAATCTGGTGTGCCTCCCTTTCGCGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTAGCCGGATGCGGCGTGAACGACTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGa                               >  1:804491/1‑150 (MQ=255)
                                                                                                                              ggAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTg                        >  1:1651666/1‑150 (MQ=255)
                                                                                                                                aaTCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTa                      >  1:1064841/1‑150 (MQ=255)
                                                                                                                                aaTCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTa                      <  1:1157156/150‑1 (MQ=255)
                                                                                                                                aaTCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTa                      >  2:1005239/1‑150 (MQ=255)
                                                                                                                                  tCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAgg                    <  2:1380339/150‑1 (MQ=255)
                                                                                                                                    tGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGcc                  >  1:421664/1‑150 (MQ=255)
                                                                                                                                    atcccttccTCCCTTTCACCCGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCAGGCTATTTTAATAAGTTGCCAGATATCCGGTAGGcc                  >  2:1135028/8‑150 (MQ=37)
                                                                                                                                     ggTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCt                 >  2:476843/1‑150 (MQ=255)
                                                                                                                                     ggTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCt                 <  1:1286411/150‑1 (MQ=255)
                                                                                                                                      gtgtGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTg                <  2:774040/150‑1 (MQ=255)
                                                                                                                                           ccTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAg           >  1:453598/1‑150 (MQ=255)
                                                                                                                                            cTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGc          >  1:1509554/1‑150 (MQ=255)
                                                                                                                                            cTCCCTTTCACCCGAAAGGGAGGAAGGGATATTAAGCAGGTGGCTGACAACGTGCGGGCTTTTTTTCCGGATGCGGCGTGAACGCCTTATCCGTCCGACAAGTTCATGCAATTTTAATAAGTATCCCGATATCCTGGAGGCCTGATAAGc          >  2:819707/1‑150 (MQ=37)
                                                                                                                                             tCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCg         <  2:1448693/150‑1 (MQ=255)
                                                                                                                                              cccTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGt        >  1:201213/1‑150 (MQ=255)
                                                                                                                                               ccTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTa       >  2:1497818/1‑150 (MQ=255)
                                                                                                                                                cTTTCGGGTGAAAGGGAGGAAGGTATATTAAGCATGTTGCTGACAACGTGCGGGCTTTTTGCCGGATGCGGCGTTAACGCCTTATCCGTCCTACTATTTCATGCAATTTTAATAAGTTGCCATATATCCTGTAGGCCTGATAAGCGTAgc     <  1:1295404/150‑1 (MQ=37)
                                                                                                                                                cTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAg      >  2:11298/1‑150 (MQ=255)
                                                                                                                                                cTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGAGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAg      <  2:1012133/150‑1 (MQ=255)
                                                                                                                                                    cGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCa  >  1:410155/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCGCGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCA  >  NC_000913/845426‑845723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: