Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 845,578 T→C 5.7% intergenic (‑98/+163) ybiO ← / ← glnQ mechanosensitive channel protein, intermediate conductance/glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913845,5780TC 5.7% 357.3 / 8.8 123intergenic (‑98/+163)ybiO/glnQmechanosensitive channel protein, intermediate conductance/glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (64/52);  new base C (5/2);  total (69/54)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 127 total aligned reads displayed.

CGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAG  >  NC_000913/845429‑845727
                                                                                                                                                     |                                                                                                                                                     
   gggCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGaa                                                                                                                                                    >  1:133191/1‑150 (MQ=255)
     gCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAg                                                                                                                                                  <  2:1062284/150‑1 (MQ=255)
      cAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCAGGACTCCTCCAGTGAGAAAATCGAGCAAAAAGCTAAGTAAAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCGGGTGTGCCTCCCTTTCGGGTGAAAgg                                                                                                                                                 <  1:739432/150‑1 (MQ=255)
        ggTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGgga                                                                                                                                               >  2:392635/1‑150 (MQ=255)
         gTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGggag                                                                                                                                              <  1:428608/150‑1 (MQ=255)
          ggcgcCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCACCCGAAAGggagg                                                                                                                                             <  1:664471/149‑1 (MQ=255)
           gcgcCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCACCCGAAAGggaggc                                                                                                                                            <  2:250338/150‑2 (MQ=255)
            cgcCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGaa                                                                                                                                           >  2:674046/1‑150 (MQ=255)
              cccAGCAGGCAGAAGAGGATGAACAGGCTCCACCGCATGACTCCGCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCGGGAGGAGGGGCGATTGTAAAAGAGAGGAATCTGGTGTGCCTCACTGTCGGGTGAAAGGtaggtagg                                                                                                                                         >  2:858821/1‑150 (MQ=255)
              cccAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACACCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTATTTGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAgg                                                                                                                                         <  1:528547/150‑1 (MQ=255)
               ccAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAggg                                                                                                                                        >  1:187763/1‑150 (MQ=255)
                 agcagGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGat                                                                                                                                      <  2:1585976/150‑1 (MQ=255)
                    aggcagAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATAtt                                                                                                                                   <  1:1189384/150‑1 (MQ=255)
                              ggATGACCACGATCCACCGCAGGACTCCTCCAGGGAGAAACTAGACCAAAAAGCAAAGTCTCGATGCTGCAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGGGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTg                                                                                                                         <  2:1136501/150‑1 (MQ=255)
                              ggATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTg                                                                                                                         >  2:735457/1‑150 (MQ=255)
                              ggATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTg                                                                                                                         >  2:1385024/1‑150 (MQ=255)
                                 tGAACATCATCCACCCCATTACACCTCCAGTGAGAAAATAGATGAAATAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTg                                                                                                                      <  1:141074/150‑1 (MQ=255)
                                 tGAACAGGATCCACCGCATGACTCCTACAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCGGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTg                                                                                                                      <  2:1209580/150‑1 (MQ=255)
                                  gAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGa                                                                                                                     >  2:544955/1‑150 (MQ=255)
                                   aaCAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGAc                                                                                                                    >  2:571530/1‑150 (MQ=255)
                                    aCAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACa                                                                                                                   >  2:1097314/1‑150 (MQ=255)
                                    aCAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATAATAATCAGGATGCTGAcc                                                                                                                   >  1:1577454/1‑149 (MQ=255)
                                      aGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAAc                                                                                                                 >  1:95439/1‑150 (MQ=255)
                                       ggATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACg                                                                                                                >  1:1609168/1‑150 (MQ=255)
                                       ggATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACg                                                                                                                >  1:1162812/1‑150 (MQ=255)
                                       ggATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACg                                                                                                                >  1:203772/1‑150 (MQ=255)
                                        gATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGt                                                                                                               >  2:1225806/1‑150 (MQ=255)
                                           ccACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCg                                                                                                            <  1:1731149/150‑1 (MQ=255)
                                           ccACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCg                                                                                                            <  1:142225/150‑1 (MQ=255)
                                              ccGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGc                                                                                                         >  2:714667/1‑150 (MQ=255)
                                               cGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACACCGTGCGGGCt                                                                                                        >  2:87766/1‑150 (MQ=255)
                                                gCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtt                                                                                                       >  2:402996/1‑150 (MQ=255)
                                                    gACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGTAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCtttttt                                                                                                   >  2:881395/1‑150 (MQ=255)
                                                        cctccAGTGAGAAAATAGAGCATAATGATTAGTTTAGATGCTGGAGGAGGGGCGATTTTTAATGAGAGGACTCTGGTGTGCCTCCCTTTCGGGTGATTGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGcgg                                                                                               <  2:1160217/150‑3 (MQ=255)
                                                         ctccAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCgg                                                                                              <  1:1686822/150‑1 (MQ=255)
                                                          tccAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGa                                                                                             <  2:461463/150‑1 (MQ=255)
                                                           ccAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGAt                                                                                            <  2:1026985/150‑1 (MQ=255)
                                                               tGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgcgg                                                                                        >  2:1029559/1‑150 (MQ=255)
                                                                 agaAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgcggcg                                                                                      <  2:797903/150‑1 (MQ=255)
                                                                 agaAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGAGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATgcggcg                                                                                      <  2:444017/150‑1 (MQ=255)
                                                                   aaaaTAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTg                                                                                    <  1:1448964/150‑1 (MQ=255)
                                                                       tAGCGCAAAAAGATAAGTATAGTTGCTGGATGAGGGGCGATTTTAAATGAGAGGAAGCGGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACg                                                                                <  2:200255/150‑1 (MQ=255)
                                                                        agagCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGc                                                                               >  1:1197407/1‑150 (MQ=255)
                                                                         gagCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGcc                                                                              <  1:818581/150‑1 (MQ=255)
                                                                         gagCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGcc                                                                              <  2:1188398/150‑1 (MQ=255)
                                                                             aaaaaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTTAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTAt                                                                          <  2:1314370/150‑1 (MQ=255)
                                                                             aaaaaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTAt                                                                          >  2:659088/1‑150 (MQ=255)
                                                                               aaaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATcc                                                                        <  2:524355/150‑1 (MQ=255)
                                                                                aaGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCg                                                                       <  1:392635/150‑1 (MQ=255)
                                                                                  gATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGATAGGGAGGAAGGGATATTACGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTc                                                                     <  1:894282/150‑1 (MQ=255)
                                                                                  gATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTc                                                                     <  2:1123255/150‑1 (MQ=255)
                                                                                   aTAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                    <  2:203772/150‑1 (MQ=255)
                                                                                   aTAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTcc                                                                    <  2:1162812/150‑1 (MQ=255)
                                                                                    taataataGATGCTGGAGGAGGGGCGATTTTAAATGAGAGCAATCTCGTGGGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCGTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCt                                                                   <  1:1646583/145‑1 (MQ=39)
                                                                                    tAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCt                                                                   <  2:1677564/150‑1 (MQ=255)
                                                                                    tAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCt                                                                   >  2:1614222/1‑150 (MQ=255)
                                                                                     aaGTATAGATGCTGGAGTAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTa                                                                  <  1:1458048/150‑1 (MQ=255)
                                                                                     aaGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTa                                                                  >  1:209461/1‑150 (MQ=255)
                                                                                       gTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCGTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACa                                                                <  1:139603/150‑1 (MQ=255)
                                                                                        tataGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                               >  1:774040/1‑150 (MQ=255)
                                                                                        tataGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCATCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGTCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACaa                                                               >  1:70221/1‑150 (MQ=255)
                                                                                           aGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGtt                                                            >  1:945349/1‑150 (MQ=255)
                                                                                            gATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTc                                                           >  1:567755/1‑150 (MQ=255)
                                                                                               gCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATg                                                        <  1:1576250/150‑1 (MQ=255)
                                                                                               gCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATg                                                        <  1:1728269/150‑1 (MQ=255)
                                                                                                  ggaggaGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCaa                                                     >  2:1671699/1‑150 (MQ=255)
                                                                                                    aggaggGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAAtt                                                   >  2:1095340/1‑150 (MQ=255)
                                                                                                      gaggGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAAtttt                                                 <  2:1356021/150‑1 (MQ=255)
                                                                                                      gaggGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAAtttt                                                 <  1:1029358/150‑1 (MQ=255)
                                                                                                          ggCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTtaata                                             >  1:1213676/1‑150 (MQ=255)
                                                                                                             gATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGTGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCTTCCTACAAGTTCATGCAATTTTAATAAGt                                          >  1:674341/1‑150 (MQ=255)
                                                                                                             gATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGt                                          <  1:1223714/150‑1 (MQ=255)
                                                                                                               ttttAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTg                                        >  1:869220/1‑150 (MQ=255)
                                                                                                                   aaaTGAGAGGAATCTGGGGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAATTCATGCAATTTTAATAAGTTGCCAg                                    <  1:1525197/150‑1 (MQ=255)
                                                                                                                    aaTGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGa                                   >  2:1517956/1‑150 (MQ=255)
                                                                                                                    aaTGAGAGGAATCTGGTGTGCCTCCCTTTCGCGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTAGCCGGATGCGGCGTGAACGACTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGa                                   >  1:804491/1‑150 (MQ=255)
                                                                                                                             aaTCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTa                          >  1:1064841/1‑150 (MQ=255)
                                                                                                                             aaTCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTa                          <  1:1157156/150‑1 (MQ=255)
                                                                                                                             aaTCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTa                          >  2:1005239/1‑150 (MQ=255)
                                                                                                                               tCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAgg                        <  2:1380339/150‑1 (MQ=255)
                                                                                                                                cTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGc                       >  2:1458792/1‑150 (MQ=255)
                                                                                                                                 tGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGcc                      >  1:421664/1‑150 (MQ=255)
                                                                                                                                 atcccttccTCCCTTTCACCCGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCAGGCTATTTTAATAAGTTGCCAGATATCCGGTAGGcc                      >  2:1135028/8‑150 (MQ=37)
                                                                                                                                  ggTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCt                     <  1:1286411/150‑1 (MQ=255)
                                                                                                                                   gtgtGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTg                    <  2:774040/150‑1 (MQ=255)
                                                                                                                                     gtgCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGAt                  <  2:1164662/150‑1 (MQ=255)
                                                                                                                                       gCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGCCAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATaa                >  1:576364/1‑150 (MQ=255)
                                                                                                                                         cTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGc              >  1:1509554/1‑150 (MQ=255)
                                                                                                                                         cTCCCTTTCACCCGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGc              >  1:1350108/1‑150 (MQ=255)
                                                                                                                                         cTCCCTTTCACCCGAAAGGGAGGAAGGGATATTAAGCAGGTGGCTGACAACGTGCGGGCTTTTTTTCCGGATGCGGCGTGAACGCCTTATCCGTCCGACAAGTTCATGCAATTTTAATAAGTATCCCGATATCCTGGAGGCCTGATAAGc              >  2:819707/1‑150 (MQ=37)
                                                                                                                                          tCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCg             <  2:1448693/150‑1 (MQ=255)
                                                                                                                                           cccTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGt            >  1:201213/1‑150 (MQ=255)
                                                                                                                                            ccTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTa           >  2:1497818/1‑150 (MQ=255)
                                                                                                                                             cTTTCGGGTGAAAGGGAGGAAGGTATATTAAGCATGTTGCTGACAACGTGCGGGCTTTTTGCCGGATGCGGCGTTAACGCCTTATCCGTCCTACTATTTCATGCAATTTTAATAAGTTGCCATATATCCTGTAGGCCTGATAAGCGTAgc         <  1:1295404/150‑1 (MQ=37)
                                                                                                                                             cTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAg          >  2:11298/1‑150 (MQ=255)
                                                                                                                                             cTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGAGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAg          <  2:1012133/150‑1 (MQ=255)
                                                                                                                                                 cGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCa      >  1:410155/1‑150 (MQ=255)
                                                                                                                                                   ggTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATc    <  2:715613/150‑1 (MQ=255)
                                                                                                                                                   ggTCAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATc    >  2:1720489/1‑150 (MQ=255)
                                                                                                                                                     tGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAg  <  1:544955/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTGGGCAGGTGCGCCCAGCAGGCAGAAGAGGATGAACAGGATCCACCGCATGACTCCTCCAGTGAGAAAATAGAGCAAAAAGATAAGTATAGATGCTGGAGGAGGGGCGATTTTAAATGAGAGGAATCTGGTGTGCCTCCCTTTCGGGTGAAAGGGAGGAAGGGATATTAAGCAGGTTGCTGACAACGTGCGGGCTTTTTTGCCGGATGCGGCGTGAACGCCTTATCCGTCCTACAAGTTCATGCAATTTTAATAAGTTGCCAGATATCCTGTAGGCCTGATAAGCGTAGCGCATCAG  >  NC_000913/845429‑845727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: