Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 72,213 C→T 7.8% intergenic (+98/+16) yabI → / ← thiQ DedA family inner membrane protein/thiamine/thiamine pyrophosphate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091372,2130CT 7.8% 314.5 / 9.9 103intergenic (+98/+16)yabI/thiQDedA family inner membrane protein/thiamine/thiamine pyrophosphate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (52/42);  new base T (3/5);  total (56/47)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACG  >  NC_000913/72066‑72361
                                                                                                                                                   |                                                                                                                                                    
cGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCaa                                                                                                                                                    >  2:168299/1‑149 (MQ=255)
 gTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATc                                                                                                                                                   <  2:1504912/150‑1 (MQ=255)
  ttGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                  <  2:467130/150‑1 (MQ=38)
  ttGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATcc                                                                                                                                                  <  1:1331930/150‑1 (MQ=255)
  tggATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                  <  2:349840/148‑1 (MQ=38)
  gtGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTAAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                  <  2:353093/149‑1 (MQ=38)
   tGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                                 <  2:1653973/150‑1 (MQ=38)
   tGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCg                                                                                                                                                 <  2:597469/150‑1 (MQ=255)
       gCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATGCGGCGTGAACGCCTTATCCGAcct                                                                                                                                             <  2:1128671/150‑3 (MQ=38)
       gCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAg                                                                                                                                             >  2:432848/1‑150 (MQ=255)
       gCCGGTGTATATCGAAATTTTGCGTAAAGTGGTGGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAg                                                                                                                                             <  1:149001/150‑1 (MQ=255)
         cGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTg                                                                                                                                           <  1:1366643/150‑1 (MQ=255)
         cGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTg                                                                                                                                           <  1:1373322/150‑1 (MQ=255)
         cGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTGTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTg                                                                                                                                           <  1:726986/150‑1 (MQ=255)
          ggTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGc                                                                                                                                          <  2:1626985/150‑1 (MQ=255)
              tataTCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATAc                                                                                                                                      <  1:1468698/150‑1 (MQ=255)
              tataTCGATAATTTGCCTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGGGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGCTCACGCCGCATCCGACAGTGCATAc                                                                                                                                      <  1:303211/150‑1 (MQ=255)
               atatCGATTTTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACt                                                                                                                                     <  2:1714705/150‑1 (MQ=255)
                  tCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAAc                                                                                                                                  >  1:1138086/1‑150 (MQ=255)
                   cGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAAcc                                                                                                                                 >  2:400493/1‑150 (MQ=255)
                   cGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAAcc                                                                                                                                 >  2:1201469/1‑150 (MQ=255)
                   cGATATTTTGCCTAACGTGGTTGGGGTTAAGGAGATACTCTTGTGCGGGTTGCCTGATGCGACGCTGGCCGCTCCCTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGAGACGGCGTTCACGCCGCATCCGACAGTGCATACTAAcc                                                                                                                                 <  1:1059113/150‑1 (MQ=38)
                    gATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAAccc                                                                                                                                >  1:1360319/1‑150 (MQ=255)
                    gATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAAccc                                                                                                                                >  1:246909/1‑150 (MQ=255)
                      aatTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGGTGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGt                                                                                                                              <  2:1369277/149‑1 (MQ=255)
                       atTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTa                                                                                                                             >  1:1131507/1‑150 (MQ=255)
                          ttGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATc                                                                                                                          <  2:40796/150‑1 (MQ=255)
                             cGTAAAGTGGTTGGGGTATAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACATTGCATACTAACCCGTAATcccc                                                                                                                       <  1:529471/150‑1 (MQ=255)
                              gTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCa                                                                                                                      <  1:7965/150‑1 (MQ=255)
                               tAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCaa                                                                                                                     >  2:474359/1‑150 (MQ=255)
                                aaaGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGTATAATGCGTTCACGCCGCTTCCGTCAGTGCATACTAACCCGTAATCCCCata                                                                                                                    >  2:837938/1‑148 (MQ=38)
                                 aaGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCCCGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATa                                                                                                                   >  2:1197263/1‑150 (MQ=255)
                                     ggTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAACCGCACTACCCGTTGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTACCCCGTAATCCCCAATAGTGc                                                                                                               >  1:192784/1‑150 (MQ=255)
                                     ggTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATTCTAACCCGTAATCCCCAATAGTGc                                                                                                               >  1:1590480/1‑150 (MQ=255)
                                     ggTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGc                                                                                                               <  1:717692/150‑1 (MQ=255)
                                       gtGGGGTTTAGGAGATAGGCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCg                                                                                                             <  1:221183/149‑1 (MQ=255)
                                         ggggTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGaa                                                                                                           <  2:819658/150‑1 (MQ=255)
                                           ggTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGc                                                                                                         <  2:710269/150‑1 (MQ=255)
                                               tAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGACGCACTc                                                                                                     >  2:837010/1‑150 (MQ=255)
                                                   agaTAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                                 >  2:1623136/1‑150 (MQ=255)
                                                   agaTAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTGCACGCCGCAGCCGACAGTTCATACTAACCCGTAATCCACAATAGTTCCGAAGCACTCGCCt                                                                                                 >  1:285289/1‑150 (MQ=38)
                                                   agaTAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGGAGGTCGGATCAGGCGTTCACGCCGACTCCGACAGTGCATACTAACCCTGAATCCCCCATAGTGCCGCAGCCCTCACCt                                                                                                 >  1:289235/1‑150 (MQ=37)
                                                      tAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTTCATAACGCACGACCCGTAGGTCGGATAAGGCGGTCACGCCGCATCCGACAGTGCATACTTACCCGGAATCCCCAGTAGTGCCGATGCACTCGCGTTAc                                                                                              >  1:882197/1‑150 (MQ=37)
                                                      tAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTAc                                                                                              >  1:1570860/1‑150 (MQ=255)
                                                          cTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCt                                                                                          <  1:103002/150‑1 (MQ=255)
                                                            tgtgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTca                                                                                        <  2:717347/150‑1 (MQ=255)
                                                                cGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGGAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTccacca                                                                                    >  1:182654/1‑148 (MQ=255)
                                                                      gCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAtt                                                                              >  1:1583328/1‑150 (MQ=255)
                                                                      gCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAtt                                                                              <  1:50678/150‑1 (MQ=255)
                                                                      gCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAtt                                                                              <  2:1570860/150‑1 (MQ=255)
                                                                        cTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAGAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTgg                                                                            >  2:1094543/1‑150 (MQ=255)
                                                                        cTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACCACTCATTgg                                                                            >  2:885940/1‑150 (MQ=255)
                                                                        cTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTgg                                                                            <  1:1276621/150‑1 (MQ=255)
                                                                        cTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTgg                                                                            >  1:1487254/1‑150 (MQ=255)
                                                                             ccGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATa                                                                       >  1:383537/2‑150 (MQ=255)
                                                                                aCGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATAccc                                                                    >  2:286749/1‑150 (MQ=255)
                                                                                      gCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGATGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAg                                                              <  1:1686313/150‑1 (MQ=255)
                                                                                       ccGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAgg                                                             <  1:7733/150‑1 (MQ=255)
                                                                                        cgcgTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGc                                                            <  1:1239835/150‑1 (MQ=255)
                                                                                        cgcgTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGc                                                            <  1:866750/150‑1 (MQ=255)
                                                                                         gcgTCTTATCAGGCCTACAAAACGCACTACCAGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCATTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCg                                                           >  1:801556/1‑150 (MQ=255)
                                                                                           gTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGAt                                                         <  1:400493/150‑1 (MQ=255)
                                                                                            tCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATg                                                        <  2:631416/150‑1 (MQ=255)
                                                                                               tATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATgcgc                                                     >  1:456334/1‑150 (MQ=255)
                                                                                               tATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACACATTGGTCATACCCTGCCAGGCGATgcgc                                                     >  2:671606/1‑150 (MQ=255)
                                                                                                  cAGGCCTACAAAACTCACTACCCGTAGGTCGGATAAGGCGTTCACGCCTCAGCCGACAGTGCAAACGAACCCGGAATCCCCAATCGTGCCGAAGCACTCGCCTGAGCGCTCAACAACTCATTGGAGATACCCAGCCCGGCGAGGCGCCCg                                                  >  2:820269/1‑150 (MQ=255)
                                                                                                  cAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCg                                                  >  1:1715600/1‑150 (MQ=255)
                                                                                                  cAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCg                                                  >  1:166869/1‑150 (MQ=255)
                                                                                                       cTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCTAAGAACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGc                                             >  2:86016/1‑150 (MQ=255)
                                                                                                       cTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGc                                             <  2:1360319/150‑1 (MQ=255)
                                                                                                        tACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCGCGCCGCATCCGACAGTGCAGACTACCCCGTAATCCCCAAAAGTGCCGAAGCACTCGCCTTACCGCTCAACACCTCAGTGGGCAGACCCGGCCCGGCGATGGCCCCGTCGGCg                                            >  1:1068699/1‑150 (MQ=255)
                                                                                                        tACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTGACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCg                                            >  2:476982/1‑150 (MQ=255)
                                                                                                           aaaaCGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGact                                         <  1:657054/150‑1 (MQ=255)
                                                                                                           aaaaCGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGact                                         >  2:1607328/1‑150 (MQ=255)
                                                                                                               cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGActacta                                     <  2:451598/150‑1 (MQ=255)
                                                                                                                gCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACtactac                                    >  1:651161/1‑150 (MQ=255)
                                                                                                                 cACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTAcc                                   <  1:1552279/150‑1 (MQ=255)
                                                                                                                     aCCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                               >  2:1599589/1‑150 (MQ=255)
                                                                                                                        gcactgTCGGATGCGGCGTGAACGCCTTATCCGACCGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCGCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGCCTACTACCGAGcgcg                            >  2:371035/6‑150 (MQ=38)
                                                                                                                         cactgTCGGATGCGGCGTGAACGCCTTATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGt                           >  1:579213/5‑150 (MQ=255)
                                                                                                                          tAGGTCGGATAAGGCGTTAACGCCGCATCCGACAGTGCATACTAACCCGTAATCACCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTg                          >  1:919074/1‑150 (MQ=255)
                                                                                                                           aGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTgg                         >  2:1673932/1‑150 (MQ=255)
                                                                                                                            ggTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGc                        <  2:246909/150‑1 (MQ=255)
                                                                                                                            ggTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGc                        <  1:1623136/150‑1 (MQ=255)
                                                                                                                             gTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACCACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCg                       >  1:810684/1‑150 (MQ=255)
                                                                                                                               cGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGAt                     >  2:658263/1‑150 (MQ=255)
                                                                                                                                ggatgcggCGTGAACGCCTTATCCGACCGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAAAAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGCATACTTCCGAGCGCGTGGCGATc                    >  2:306620/7‑150 (MQ=255)
                                                                                                                                ggATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATc                    >  2:1627270/1‑150 (MQ=255)
                                                                                                                                  aTAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCcg                  >  1:671501/1‑150 (MQ=255)
                                                                                                                                  aTAAGGCGTTCACGCCGCATACGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTCCCGCTCAACAACTCCTTGGTCATACACTGCCAGGCGATGCGCCCGTCGGCGACAACTACCGAGCGCGTGGCGATCcg                  >  1:1156593/1‑150 (MQ=255)
                                                                                                                                    ttgccGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCcgcg                <  2:74396/146‑1 (MQ=255)
                                                                                                                                    aaGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCcgcg                >  2:1376365/1‑150 (MQ=255)
                                                                                                                                      ggCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCACCAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgcc              >  1:316397/1‑150 (MQ=255)
                                                                                                                                      ggCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCCTACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTAACGAGCGCGTGGCGATCCGcgcc              >  2:316408/1‑150 (MQ=255)
                                                                                                                                      ggCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGcgcc              >  1:1378151/1‑150 (MQ=255)
                                                                                                                                           tCACGCCGCATCCGACAGTGCATACTAACCCGAAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCCGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATc         <  2:677153/150‑1 (MQ=255)
                                                                                                                                            cACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCt        <  2:1583328/150‑1 (MQ=255)
                                                                                                                                            cACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGAAGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCAACt        >  1:464222/1‑150 (MQ=255)
                                                                                                                                               gccgcATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTcc     <  1:1201469/150‑1 (MQ=255)
                                                                                                                                                ccgcATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTccc    >  2:1235669/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACG  >  NC_000913/72066‑72361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: