Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 852,857 A→C 7.1% intergenic (‑260/+12) opgE ← / ← mntS OPG biosynthetic transmembrane phosphoethanolamine transferase/Mn(2)‑response protein, MntR‑repressed

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913852,8570AC 7.1% 375.2 / 15.3 126intergenic (‑260/+12)opgE/mntSOPG biosynthetic transmembrane phosphoethanolamine transferase/Mn(2)‑response protein, MntR‑repressed
Reads supporting (aligned to +/- strand):  ref base A (52/65);  new base C (4/5);  total (56/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 127 total aligned reads displayed.

GGGCTGCAAGAAGATAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGACC  >  NC_000913/852710‑853003
                                                                                                                                                   |                                                                                                                                                  
 ggCTGCAAGAAGATAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACcg                                                                                                                                                 <  1:1143820/150‑1 (MQ=255)
 ggCTGCAAGAAGATAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACcgc                                                                                                                                                >  2:580285/1‑150 (MQ=255)
  gCTGCAAGAAGATAGAGCGAGCCGCCGTTCAGGTGAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGAGTACcgc                                                                                                                                                >  1:92265/1‑150 (MQ=255)
  gCTGCAAGAAGATAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACcgc                                                                                                                                                <  1:501329/150‑1 (MQ=255)
      cAAGAAGATAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGAcg                                                                                                                                            >  2:1325880/1‑150 (MQ=255)
         gaagaTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGAcgccg                                                                                                                                         >  1:1297175/1‑150 (MQ=255)
          aagaTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGCACAAATCTTTTGCGAAGGGGATTGCAAAGCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGAcgccgc                                                                                                                                        >  2:1047578/1‑150 (MQ=255)
           agaTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTCGCGGTACGCCGCt                                                                                                                                       <  1:373878/150‑1 (MQ=255)
           agaTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCt                                                                                                                                       >  1:1704997/1‑150 (MQ=255)
            gaTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTCGCGGTACGCCGCtt                                                                                                                                      <  1:1498649/150‑2 (MQ=255)
             aTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTCGCGGTACGCCGCttc                                                                                                                                     <  2:294637/150‑3 (MQ=255)
             aTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTAt                                                                                                                                     >  2:201010/1‑150 (MQ=255)
              tAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTCGCGGTACGCCGCttca                                                                                                                                    <  1:458659/150‑4 (MQ=255)
              tAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGGTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTAtt                                                                                                                                    <  1:1400436/150‑1 (MQ=255)
                gagcgagcCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTa                                                                                                                                  <  1:1006968/150‑1 (MQ=255)
                gagcgagcCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTa                                                                                                                                  >  1:516861/1‑150 (MQ=255)
                   cgagcCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCg                                                                                                                               <  1:1360057/150‑1 (MQ=255)
                       ccgccgTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAg                                                                                                                           >  2:95203/1‑150 (MQ=255)
                         gccgTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGt                                                                                                                         >  2:782139/1‑150 (MQ=255)
                          ccgTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGtt                                                                                                                        <  1:69619/150‑1 (MQ=255)
                           ggTTCAGGTCAACTACTGACGTACAAATCTTTTGCGAAGGGTATTGCAAATCCGACAAAAAGGGTGGTTTTTCAGTCCAAAACTCGTTATGCGTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGttt                                                                                                                       <  1:180540/149‑1 (MQ=255)
                           cgTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGttt                                                                                                                       <  2:1704997/150‑1 (MQ=255)
                           cgTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGttt                                                                                                                       <  2:651286/150‑1 (MQ=255)
                             ttCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTAt                                                                                                                     <  1:1064457/150‑1 (MQ=255)
                               cAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCt                                                                                                                   >  1:1693867/1‑150 (MQ=255)
                                  gTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTtgc                                                                                                                <  1:1142972/150‑1 (MQ=255)
                                  gTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTtga                                                                                                                >  1:1385907/1‑149 (MQ=255)
                                     aaCTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTtgctgc                                                                                                             <  2:499904/150‑1 (MQ=255)
                                      actactGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCg                                                                                                            <  2:99417/150‑1 (MQ=255)
                                         actGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGtt                                                                                                         <  2:1570190/150‑1 (MQ=255)
                                            gACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgt                                                                                                      <  2:1140970/150‑1 (MQ=255)
                                            gACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgt                                                                                                      >  1:1537080/1‑150 (MQ=255)
                                             aCGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgtt                                                                                                     <  1:1666446/150‑1 (MQ=255)
                                              cGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACTCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttg                                                                                                    <  1:1568734/150‑1 (MQ=255)
                                              cGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttg                                                                                                    <  2:788234/150‑1 (MQ=255)
                                               gAACAAATCTTTTGCGAAGGGGATTGCATATCCGACAAAAAGTGGGGTTTTTCAGACCTAAACGCGTTATTCCTGCATGAAAGAAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttgt                                                                                                   <  1:277588/150‑1 (MQ=255)
                                                aaCAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttgtt                                                                                                  >  2:250704/1‑150 (MQ=255)
                                                aaCAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttgtt                                                                                                  <  2:1668195/150‑1 (MQ=255)
                                                aaCAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttgtt                                                                                                  >  1:466789/1‑150 (MQ=255)
                                                  cAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGa                                                                                                >  1:1656980/1‑150 (MQ=255)
                                                   aaaTCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGAc                                                                                               >  1:318446/1‑150 (MQ=255)
                                                          ttGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCg                                                                                        <  1:238365/150‑1 (MQ=255)
                                                             cGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGcac                                                                                     <  2:529287/150‑1 (MQ=255)
                                                             cGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGcac                                                                                     <  1:1157735/150‑1 (MQ=255)
                                                              gAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGcaca                                                                                    >  2:830711/1‑150 (MQ=255)
                                                              gAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGcaca                                                                                    >  2:1067308/1‑150 (MQ=255)
                                                                 ggggATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAATGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCacaaca                                                                                 <  2:609241/150‑1 (MQ=255)
                                                                   ggATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATa                                                                               <  1:562043/150‑1 (MQ=255)
                                                                    gATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATag                                                                              <  1:722833/150‑1 (MQ=255)
                                                                    gATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATag                                                                              <  1:743527/150‑1 (MQ=255)
                                                                    gATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATag                                                                              >  2:548472/1‑150 (MQ=255)
                                                                    gATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATag                                                                              <  2:140197/150‑1 (MQ=255)
                                                                        gCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGCCGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACTATATCGCACAACATagagag                                                                          >  1:1600308/1‑150 (MQ=255)
                                                                        gCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACagagagag                                                                          >  1:947403/1‑150 (MQ=255)
                                                                        gCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATagagag                                                                          >  2:1687749/1‑150 (MQ=255)
                                                                         cAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagc                                                                         <  1:672407/150‑1 (MQ=255)
                                                                           aaTCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagcag                                                                       <  2:1388319/150‑1 (MQ=255)
                                                                            aTCCGACAAAACGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagcagc                                                                      <  1:1443938/150‑1 (MQ=255)
                                                                                gACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTa                                                                  >  2:503551/1‑150 (MQ=255)
                                                                                gACAAAAACTGGGGGGTTTCAGCCCAAAACGCGTTATGCCCGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTa                                                                  <  2:261425/150‑1 (MQ=255)
                                                                                 aCAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTaa                                                                 >  2:598134/1‑150 (MQ=255)
                                                                                    aaaaGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCg                                                              >  1:347453/1‑150 (MQ=255)
                                                                                    aaaaGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCg                                                              <  1:179249/150‑1 (MQ=255)
                                                                                    aaaaGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCg                                                              <  1:201010/150‑1 (MQ=255)
                                                                                    aaaaGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCg                                                              >  1:1326621/1‑150 (MQ=255)
                                                                                          ggggTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAATCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACttt                                                        <  2:1537080/150‑1 (MQ=255)
                                                                                                  tCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGAGCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGgat                                                >  2:28264/1‑149 (MQ=255)
                                                                                                  tCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGgag                                                <  1:580285/150‑1 (MQ=255)
                                                                                                   cAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGgaga                                               >  1:1468955/1‑150 (MQ=255)
                                                                                                     gACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAAt                                             <  2:466789/150‑1 (MQ=255)
                                                                                                     gACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAAt                                             >  1:1430985/1‑150 (MQ=255)
                                                                                                       ccAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGa                                           >  1:671735/1‑150 (MQ=255)
                                                                                                        cAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTTTCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGAc                                          >  2:1291740/1‑150 (MQ=255)
                                                                                                         aaaaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACt                                         >  2:1290721/1‑150 (MQ=255)
                                                                                                           aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTaa                                       <  2:516861/150‑1 (MQ=255)
                                                                                                             cgcgTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGACGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAac                                     >  2:141562/1‑150 (MQ=255)
                                                                                                                  tATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACAcgcg                                >  2:1615465/1‑150 (MQ=255)
                                                                                                                    tGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCa                              <  2:363660/150‑1 (MQ=255)
                                                                                                                    tGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCa                              >  1:731352/1‑150 (MQ=255)
                                                                                                                    tGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATATATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGAGCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCa                              <  2:670341/150‑1 (MQ=255)
                                                                                                                       cTGCATGAAAGCAGGAGTGAAGCGGGGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCGGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATac                           <  2:92265/150‑1 (MQ=255)
                                                                                                                       cTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATac                           <  2:1693867/150‑1 (MQ=255)
                                                                                                                          cATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACAcc                        <  1:1619119/150‑1 (MQ=255)
                                                                                                                          cATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACAcc                        <  1:1226318/150‑1 (MQ=255)
                                                                                                                          cATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACAAAACACGCGCATACAcc                        >  2:1177595/1‑150 (MQ=255)
                                                                                                                           aTGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACct                       <  2:1359786/150‑1 (MQ=255)
                                                                                                                             gAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACctct                     >  2:1359045/1‑150 (MQ=255)
                                                                                                                                  cAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAAc                <  1:1467664/150‑1 (MQ=255)
                                                                                                                                      taaataGCGGCGTCGCGGTACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCTCAACATAGAGAGCAGCAGTAACCGTACTTTTAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcat            >  1:720589/6‑150 (MQ=255)
                                                                                                                                       gTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATTCACCTCTTGAACtcatt           >  1:1005791/1‑150 (MQ=255)
                                                                                                                                       gTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcatt           >  1:687426/1‑150 (MQ=255)
                                                                                                                                       gTGAAGCGGCGTACCGCGACGCCGCGATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCAGAACATAGAGAGCCGCATTACCCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGATCtcatt           >  1:1080160/1‑150 (MQ=255)
                                                                                                                                        tGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACCCCTCTTGAACtcattc          >  2:1274404/1‑150 (MQ=255)
                                                                                                                                        tGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcattc          <  1:309450/150‑1 (MQ=255)
                                                                                                                                         gAAGCGGCGTACCGCGACGCCGCTATTTATCGTAATGTTTACCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcattca         <  2:653058/150‑1 (MQ=255)
                                                                                                                                         gAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcattca         <  2:318446/150‑1 (MQ=255)
                                                                                                                                         ataGCGGCGTCGCGGTACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcattca         >  2:1156244/3‑150 (MQ=255)
                                                                                                                                           aGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATa       <  1:598134/150‑1 (MQ=255)
                                                                                                                                             cggcgTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAg     <  1:801620/150‑1 (MQ=255)
                                                                                                                                                cgTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGAcc  <  1:95203/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGCTGCAAGAAGATAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGACC  >  NC_000913/852710‑853003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: