Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 78,830 T→A 6.2% intergenic (+31/+18) setA → / ← leuD broad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091378,8300TA 6.2% 312.4 / 6.3 112intergenic (+31/+18)setA/leuDbroad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit
Reads supporting (aligned to +/- strand):  ref base T (53/51);  new base A (3/4);  total (56/56)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 112 total aligned reads displayed.

GGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGG  >  NC_000913/78686‑78978
                                                                                                                                                |                                                                                                                                                     
ggcGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGAc                                                                                                                                                  <  2:721375/150‑1 (MQ=255)
ggcGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGAc                                                                                                                                                  <  2:1718121/150‑1 (MQ=255)
     tATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGc                                                                                                                                             >  2:888938/1‑150 (MQ=255)
     tATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                                                                             <  1:1576038/150‑1 (MQ=255)
     tATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACAGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGc                                                                                                                                             >  1:97046/1‑150 (MQ=255)
      aTTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGct                                                                                                                                            <  2:1385979/150‑2 (MQ=255)
      aTTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGAATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAACCCCGGTCATTTGACCGGGCa                                                                                                                                            >  1:1264763/1‑150 (MQ=255)
       ttCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTATGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGctt                                                                                                                                           <  2:1220105/150‑3 (MQ=255)
       ttCAGGCAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTAAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGctt                                                                                                                                           <  2:1137141/150‑3 (MQ=255)
          aGGGAGCAATTGCCCAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAggg                                                                                                                                       <  2:895970/150‑1 (MQ=255)
          aGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAggg                                                                                                                                       >  2:1062601/1‑150 (MQ=255)
           gggAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAgggg                                                                                                                                      >  1:769024/1‑150 (MQ=255)
           gggAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAgggg                                                                                                                                      >  2:1186595/1‑150 (MQ=255)
            ggAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGa                                                                                                                                     >  2:438175/1‑150 (MQ=255)
                 aaTTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAATACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTAGACCGGGCAAGTGGATTAAt                                                                                                                                >  1:1293502/1‑150 (MQ=255)
                 aaTTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAAt                                                                                                                                <  1:613210/150‑1 (MQ=255)
                  aTTGCACAAAGTGGGGGGCACGTTGCGGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATAATTTTTAACCGCAAAGGTTAAAGACGGTTGATGACGTGGACGATAGCGGAAAGCCCGGGCATTTGACCGGGCAAGGGGATTAAtt                                                                                                                               <  2:1452419/150‑1 (MQ=255)
                    tGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCa                                                                                                                             <  1:1186595/150‑1 (MQ=255)
                     gCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCAt                                                                                                                            <  2:568853/150‑1 (MQ=255)
                     gCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGGTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCAt                                                                                                                            <  2:1043171/150‑1 (MQ=255)
                      cacaAAGTTGGTGGCACTTTGCTGTCTACTTGGTAATTGCGGTTATTTCTGTTCTCGCATTATTTTTAACCGCAAATGTTATAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATa                                                                                                                           <  2:945724/150‑1 (MQ=255)
                      cacaAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATa                                                                                                                           <  2:885131/150‑1 (MQ=255)
                      cacaAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATa                                                                                                                           <  1:1023164/150‑1 (MQ=255)
                      cacaAAGTTGGGGGCACTTTGCTGGCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGCGTGATTAAttaac                                                                                                                            <  1:275595/150‑4 (MQ=255)
                         aaaGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAAc                                                                                                                        >  1:1618426/1‑150 (MQ=255)
                            gTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGTAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCa                                                                                                                     >  2:1011157/1‑150 (MQ=255)
                            gTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCa                                                                                                                     <  2:1633189/150‑1 (MQ=255)
                               gggggCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTc                                                                                                                              >  2:1264983/1‑138 (MQ=255)
                                ggggCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgtt                                                                                                                 >  2:432239/1‑150 (MQ=255)
                                      cTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTg                                                                                                           <  1:628233/150‑1 (MQ=255)
                                      cTTTGCGGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTg                                                                                                           <  2:699592/150‑1 (MQ=255)
                                        ttGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCt                                                                                                         <  1:685091/150‑1 (MQ=255)
                                          gCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTc                                                                                                       <  2:700592/150‑1 (MQ=255)
                                            ggTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCAt                                                                                                     <  1:1422503/149‑1 (MQ=255)
                                             gTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGTAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATa                                                                                                    <  1:1011157/150‑1 (MQ=255)
                                                 aCTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCg                                                                                                >  2:808518/1‑150 (MQ=255)
                                                    gggTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCa                                                                                             >  2:1702322/1‑150 (MQ=255)
                                                      gTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAAt                                                                                           <  2:1678374/150‑1 (MQ=255)
                                                        aaTTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATgg                                                                                         >  1:504791/1‑150 (MQ=255)
                                                         tttGCGGGTATTTCTGATGTCGCATTATTTTTATCCGCAAAGTTTAAAGACGTTTGCTGCCGTGGACGATAGCGGAAAGCCCGGTCATTTGCCCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGc                                                                                        <  2:315698/149‑1 (MQ=255)
                                                          ttGCTGTTATTTCTGTTGTCTCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcg                                                                                       <  1:967637/150‑1 (MQ=255)
                                                          ttGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcg                                                                                       <  2:463773/150‑1 (MQ=255)
                                                             cGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcgtcg                                                                                    >  1:898002/1‑150 (MQ=255)
                                                              ggTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcgtcgt                                                                                   >  1:50128/1‑150 (MQ=255)
                                                               gTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcgtcgtc                                                                                  >  2:343955/1‑150 (MQ=255)
                                                                ttATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcgtcgtcg                                                                                 <  1:1163050/150‑1 (MQ=255)
                                                                ttATTTCTGTTGGCGCATTTTTATTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAATCGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcgtcgtcg                                                                                 <  1:1464743/150‑1 (MQ=255)
                                                                           gtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCTGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGt                                                                      >  2:1520561/1‑150 (MQ=255)
                                                                              gCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGGCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGGTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAg                                                                   <  1:342139/150‑1 (MQ=255)
                                                                                attattTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGCCCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGcc                                                                 >  2:1570046/1‑150 (MQ=255)
                                                                                attattTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGTCGTCGTCGTGCTGCAAGGTAAGcc                                                                 >  1:1564303/1‑150 (MQ=255)
                                                                                attattTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGcc                                                                 >  2:549673/1‑150 (MQ=255)
                                                                                 ttattTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTTATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGccc                                                                >  1:1352602/1‑150 (MQ=255)
                                                                                 ttattTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTGCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGccc                                                                >  1:1208221/1‑150 (MQ=255)
                                                                                 ttattTTTAACCGCAAAGGGGAAAGACGTTTGATGCCGTGGACGATAGCGGAAAGCACGGTCACTTGACCGGGCAAGGGGATTACTTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGccc                                                                <  2:1157152/150‑1 (MQ=255)
                                                                                  tattTTTAACCGCAAAGGTTAAAGCCGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTTATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCa                                                               >  1:20983/1‑150 (MQ=255)
                                                                                   attTTTAACCGCAAAGGTTAAAGACGTTTGATGTCGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCaa                                                              <  1:606606/150‑1 (MQ=255)
                                                                                    tttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAAt                                                             >  1:1424014/1‑150 (MQ=255)
                                                                                    tttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAAt                                                             <  1:1062601/150‑1 (MQ=255)
                                                                                     ttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCTAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGGGTCGTCGTGCTGCAAGGTAAGCCCAATa                                                            >  1:59446/1‑150 (MQ=255)
                                                                                      tttAACCGCTAAGGTTAAAGTCGTTTGATGCTGTGGACGACAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATGAAATCATAAACGCAGGTTGGTGTGCTTCATAAGCGGCAATGGCGGCGTCGTGCTGCAAGGTAAGCCCAATAc                                                           <  1:1562468/150‑1 (MQ=255)
                                                                                      tttAACCGCAAAGGTTATATACGTTTGATGTCGTGGACGATAGCGGACAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCTTCGTCGTGCTGCAAGGTAAGCCCAATAc                                                           <  2:1317305/150‑1 (MQ=255)
                                                                                      tttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAc                                                           >  2:1140386/1‑150 (MQ=255)
                                                                                      tttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAc                                                           >  1:1386262/1‑150 (MQ=255)
                                                                                         aaCCGCAAAGGTTAAAGACGTTTGATGACTTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAATGTAAGCCCAATACTGt                                                        <  1:733423/150‑1 (MQ=255)
                                                                                          aCCGCAAAGGTTAAAGAAGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTc                                                       >  2:1297276/1‑150 (MQ=255)
                                                                                           ccGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTTTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTcc                                                      <  1:1353060/150‑1 (MQ=255)
                                                                                           ccGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTcc                                                      <  2:668203/150‑1 (MQ=255)
                                                                                           ccGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTcc                                                      <  2:400828/150‑1 (MQ=255)
                                                                                           ccGCAAAGGTTAAAGACGTTTGATGACGAGGACGATAGCGGAAAGCCCGGTCATTTGCCCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTcc                                                      <  2:841085/150‑1 (MQ=255)
                                                                                             gCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAg                                                    >  1:710659/1‑150 (MQ=255)
                                                                                              cAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAgc                                                   >  1:386088/1‑149 (MQ=255)
                                                                                                   gTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCACGGTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGtt                                              <  2:353856/150‑1 (MQ=255)
                                                                                                     tAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTtca                                            >  1:399443/1‑150 (MQ=255)
                                                                                                      aaaGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTtcat                                           <  2:97046/150‑1 (MQ=255)
                                                                                                      aaaGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTtcat                                           >  2:201033/1‑150 (MQ=255)
                                                                                                      aaaGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTtcat                                           >  2:428444/1‑150 (MQ=255)
                                                                                                       aaGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTtcatc                                          <  2:518121/150‑1 (MQ=255)
                                                                                                       aaGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTtcatc                                          >  2:1367169/1‑150 (MQ=255)
                                                                                                         gACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTcatcat                                        >  1:590102/1‑150 (MQ=255)
                                                                                                                         gACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAg                        <  2:1472137/150‑1 (MQ=255)
                                                                                                                          aCGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAgg                       <  2:321242/150‑1 (MQ=255)
                                                                                                                             aTAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCAAGACCGTTCATCATGCAGTGGCGGCGGAAGGCAt                    >  1:1542425/1‑150 (MQ=255)
                                                                                                                              tAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGGCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATc                   <  2:474497/150‑1 (MQ=255)
                                                                                                                                tccccttgCCCGGTCAAATGCCCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGa                 >  2:779886/8‑150 (MQ=255)
                                                                                                                                gCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTTTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGa                 >  2:65104/1‑150 (MQ=255)
                                                                                                                                 ccccttgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCACACCGTTCATCATGCAGTGGCGGCGGATGGCTTCGAt                >  2:947020/7‑150 (MQ=255)
                                                                                                                                  ggAAAGCCCGGTCTTTTGACCGGGCAAGGGGATTATTTCATACACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGGCGTCGTGCTGCAAGGTAAGCCCTATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATg               <  1:20596/150‑1 (MQ=255)
                                                                                                                                    aaaGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTGTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCTGCCCGTTCATCGTGCAGTGGGGGCGGAAGGCATCGCTGGt             >  2:1172596/1‑150 (MQ=255)
                                                                                                                                     aaGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTa            <  1:1234363/150‑1 (MQ=255)
                                                                                                                                     aaGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTa            <  1:1217978/150‑1 (MQ=255)
                                                                                                                                      aGCCCGGTCATTTGACCGGGCTAGGGGATTAATTCATAATCGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGAGCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTaa           >  1:265197/1‑150 (MQ=255)
                                                                                                                                       gCCCGGTCAATTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTGGTTTTGCTTCATTAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCGAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCCGAAGGCATAGAAGGaaaa          >  1:350490/1‑150 (MQ=255)
                                                                                                                                          cGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCg       >  2:1709108/1‑150 (MQ=255)
                                                                                                                                            gTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGTAAGGCATCGATGGTAAAGCGAt     >  2:1496764/1‑150 (MQ=255)
                                                                                                                                              cATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAg   <  1:1325593/150‑1 (MQ=255)
                                                                                                                                              cATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAATGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAg   >  1:872359/1‑150 (MQ=255)
                                                                                                                                              cATTTGACCGGGCAAGGGGAGTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGATCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAg   <  1:432239/150‑1 (MQ=255)
                                                                                                                                               aTTTGCCCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCTGTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAgg  >  2:1574253/1‑150 (MQ=255)
                                                                                                                                               aTTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAgg  >  1:1513296/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGACCGTTCATCATGCAGTGGCGGCGGAAGGCATCGATGGTAAAGCGATAGG  >  NC_000913/78686‑78978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: