Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 852,862 G→T 7.4% intergenic (‑265/+7) opgE ← / ← mntS OPG biosynthetic transmembrane phosphoethanolamine transferase/Mn(2)‑response protein, MntR‑repressed

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913852,8620GT 7.4% 355.5 / 13.6 122intergenic (‑265/+7)opgE/mntSOPG biosynthetic transmembrane phosphoethanolamine transferase/Mn(2)‑response protein, MntR‑repressed
Reads supporting (aligned to +/- strand):  ref base G (50/63);  new base T (4/5);  total (54/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

Only 100 of 124 total aligned reads displayed.

CAAGAAGATAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGACCTCCTGACT  >  NC_000913/852716‑853011
                                                                                                                                                  |                                                                                                                                                     
cAAGAAGATAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGAcg                                                                                                                                                    >  2:588470/1‑150 (MQ=255)
    aagaTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGCACAAATCTTTTGCGAAGGGGATTGCAAAGCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGAcgccgc                                                                                                                                                >  2:1047578/1‑150 (MQ=255)
     agaTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTCGCGGTACGCCGCt                                                                                                                                               <  1:373878/150‑1 (MQ=255)
     agaTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCt                                                                                                                                               >  1:1704997/1‑150 (MQ=255)
      gaTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTCGCGGTACGCCGCtt                                                                                                                                              <  1:1498649/150‑2 (MQ=255)
       aTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCttc                                                                                                                                             <  1:588470/150‑3 (MQ=255)
       aTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTAt                                                                                                                                             >  2:201010/1‑150 (MQ=255)
       aTAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTAt                                                                                                                                             >  1:1388319/1‑150 (MQ=255)
        tAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTCGCGGTACGCCGCttca                                                                                                                                            <  1:458659/150‑4 (MQ=255)
          gagcgagcCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTa                                                                                                                                          <  1:1006968/150‑1 (MQ=255)
          gagcgagcCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTa                                                                                                                                          >  1:516861/1‑150 (MQ=255)
            gcgagcCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATc                                                                                                                                        <  2:450840/150‑1 (MQ=255)
            ccgagcCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGACGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATc                                                                                                                                        <  1:694020/149‑1 (MQ=255)
             cgagcCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCg                                                                                                                                       <  1:1360057/150‑1 (MQ=255)
                 ccgccgTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAg                                                                                                                                   >  2:95203/1‑150 (MQ=255)
                   gccgTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGt                                                                                                                                 >  2:782139/1‑150 (MQ=255)
                     ggTTCAGGTCAACTACTGACGTACAAATCTTTTGCGAAGGGTATTGCAAATCCGACAAAAAGGGTGGTTTTTCAGTCCAAAACTCGTTATGCGTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGttt                                                                                                                               <  1:180540/149‑1 (MQ=255)
                     cgTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGttt                                                                                                                               <  2:651286/150‑1 (MQ=255)
                       ttCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTAt                                                                                                                             <  1:1064457/150‑1 (MQ=255)
                        tCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATc                                                                                                                            <  2:1348487/150‑1 (MQ=255)
                           ggacAACTACTGACGAACAAATCATTTGCGAAGGGGATTGCAAATACGACAAAAAGTGTGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTtg                                                                                                                         <  2:1290394/147‑1 (MQ=255)
                            gTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTtgc                                                                                                                        <  1:1142972/150‑1 (MQ=255)
                            gTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTtga                                                                                                                        >  1:1385907/1‑149 (MQ=255)
                            gTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTAATTATCGGAAGGTTTATCTtgc                                                                                                                        >  1:252943/1‑150 (MQ=255)
                             tCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTtgct                                                                                                                       <  2:1721753/150‑1 (MQ=255)
                                actactGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCg                                                                                                                    <  2:99417/150‑1 (MQ=255)
                                   actGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGtt                                                                                                                 <  2:1570190/150‑1 (MQ=255)
                                      gACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTCGCGGTACGCCGCtt                                                                                                                                              >  2:1113295/1‑117 (MQ=255)
                                      gACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgt                                                                                                              <  2:1140970/150‑1 (MQ=255)
                                      gACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgt                                                                                                              >  1:1537080/1‑150 (MQ=255)
                                       aCGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgtt                                                                                                             <  1:1666446/150‑1 (MQ=255)
                                        cGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACTCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttg                                                                                                            <  1:1568734/150‑1 (MQ=255)
                                        cGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttg                                                                                                            <  2:788234/150‑1 (MQ=255)
                                         gAACAAATCTTTTGCGAAGGGGATTGCATATCCGACAAAAAGTGGGGTTTTTCAGACCTAAACGCGTTATTCCTGCATGAAAGAAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttgt                                                                                                           <  1:277588/150‑1 (MQ=255)
                                          aaCAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttgtt                                                                                                          >  2:250704/1‑150 (MQ=255)
                                          aaCAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttgtt                                                                                                          <  2:1668195/150‑1 (MQ=255)
                                          aaCAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTttgttgtt                                                                                                          >  1:466789/1‑150 (MQ=255)
                                            cAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGa                                                                                                        >  1:1656980/1‑150 (MQ=255)
                                             aaaTCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGAc                                                                                                       >  1:1278167/1‑150 (MQ=255)
                                             aaaTCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGAc                                                                                                       >  1:318446/1‑150 (MQ=255)
                                                       cGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGcac                                                                                             <  1:1157735/150‑1 (MQ=255)
                                                       cGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGcac                                                                                             <  2:529287/150‑1 (MQ=255)
                                                        gAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGcaca                                                                                            <  1:735114/150‑1 (MQ=255)
                                                           ggggATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAATGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCacaaca                                                                                         <  2:609241/150‑1 (MQ=255)
                                                             ggATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATa                                                                                       <  1:562043/150‑1 (MQ=255)
                                                              gATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATag                                                                                      <  1:722833/150‑1 (MQ=255)
                                                                  gCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACagagagag                                                                                  >  1:947403/1‑150 (MQ=255)
                                                                  gCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATagagag                                                                                  >  2:1687749/1‑150 (MQ=255)
                                                                   cAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagc                                                                                 <  1:672407/150‑1 (MQ=255)
                                                                     aaTCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagcag                                                                               <  2:1388319/150‑1 (MQ=255)
                                                                      aTCCGACAAAACGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagcagc                                                                              <  1:1443938/150‑1 (MQ=255)
                                                                      aTCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGagcagc                                                                              <  1:1450694/150‑1 (MQ=255)
                                                                        ccGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCAt                                                                            <  1:943792/150‑1 (MQ=255)
                                                                          gACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTa                                                                          >  2:503551/1‑150 (MQ=255)
                                                                          gACAAAAACTGGGGGGTTTCAGCCCAAAACGCGTTATGCCCGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTa                                                                          <  2:261425/150‑1 (MQ=255)
                                                                           aCAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTaa                                                                         >  2:598134/1‑150 (MQ=255)
                                                                              aaaaGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCg                                                                      >  1:1326621/1‑150 (MQ=255)
                                                                              aaaaGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCg                                                                      <  1:179249/150‑1 (MQ=255)
                                                                              aaaaGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCg                                                                      <  1:201010/150‑1 (MQ=255)
                                                                              aaaaGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCg                                                                      >  1:1562993/1‑150 (MQ=255)
                                                                                   gggggTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGGGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACtt                                                                 <  2:1406545/149‑1 (MQ=255)
                                                                                    ggggTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAATCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACttt                                                                <  2:1537080/150‑1 (MQ=255)
                                                                                            tCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGAGCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGgat                                                        >  2:28264/1‑149 (MQ=255)
                                                                                            tCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGgag                                                        <  1:580285/150‑1 (MQ=255)
                                                                                             cAGACAAAAACGCGTTAACCCTGCATGAAAGCAGGAGTGAACCGGCGTACCGCGACGCCGCTATTTATCAGAAGGTTTATCTAGCTGCGGTTTGTTGTTGACCATATCTCACAACATATAGAGAAGCAATAAAACTACTTTAAAGCgaga                                                       >  2:913710/1‑150 (MQ=255)
                                                                                               gACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAAt                                                     >  1:1430985/1‑150 (MQ=255)
                                                                                               gACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAAt                                                     <  2:466789/150‑1 (MQ=255)
                                                                                                 ccAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGa                                                   >  1:671735/1‑150 (MQ=255)
                                                                                                  cAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTTTCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGAc                                                  >  2:1291740/1‑150 (MQ=255)
                                                                                                   aaaaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACt                                                 >  2:1290721/1‑150 (MQ=255)
                                                                                                     aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTaa                                               >  1:1658772/1‑150 (MQ=255)
                                                                                                     aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTaa                                               <  2:516861/150‑1 (MQ=255)
                                                                                                     aaCGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTaa                                               >  2:528026/1‑150 (MQ=255)
                                                                                                       cgcgTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGACGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAac                                             >  2:141562/1‑150 (MQ=255)
                                                                                                            tATGCCTGCATGAAATCAGGAGTGAAGCGGCGTACCGCGTCGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACAcgcg                                        <  1:357520/150‑1 (MQ=255)
                                                                                                              tGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCa                                      >  1:731352/1‑150 (MQ=255)
                                                                                                              tGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCa                                      <  2:363660/150‑1 (MQ=255)
                                                                                                              tGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATATATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGAGCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCa                                      <  2:670341/150‑1 (MQ=255)
                                                                                                                ccTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTAGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGTAGAATGACTAAACACGCGCATa                                    >  2:664124/1‑150 (MQ=255)
                                                                                                                 cTGCATGAAAGCAGGAGTGAAGCGGGGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCGGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATac                                   <  2:92265/150‑1 (MQ=255)
                                                                                                                 cTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATac                                   <  2:1693867/150‑1 (MQ=255)
                                                                                                                    cATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACAcc                                <  1:1619119/150‑1 (MQ=255)
                                                                                                                    cATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACAcc                                <  1:1226318/150‑1 (MQ=255)
                                                                                                                    cATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACAAAACACGCGCATACAcc                                >  2:1177595/1‑150 (MQ=255)
                                                                                                                     aTGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACct                               <  2:1359786/150‑1 (MQ=255)
                                                                                                                       gAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACctct                             >  2:1359045/1‑150 (MQ=255)
                                                                                                                         aaGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTg                           <  2:1656980/150‑1 (MQ=255)
                                                                                                                            cAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAAc                        <  1:1467664/150‑1 (MQ=255)
                                                                                                                                taaataGCGGCGTCGCGGTACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCTCAACATAGAGAGCAGCAGTAACCGTACTTTTAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcat                    >  1:720589/6‑150 (MQ=255)
                                                                                                                                 gTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATTCACCTCTTGAACtcatt                   >  1:1005791/1‑150 (MQ=255)
                                                                                                                                 gTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcatt                   >  1:687426/1‑150 (MQ=255)
                                                                                                                                 gTGAAGCGGCGTACCGCGACGCCGCGATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCAGAACATAGAGAGCCGCATTACCCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGATCtcatt                   >  1:1080160/1‑150 (MQ=255)
                                                                                                                                  tGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACCCCTCTTGAACtcattc                  >  2:1274404/1‑150 (MQ=255)
                                                                                                                                  tGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcattc                  <  1:309450/150‑1 (MQ=255)
                                                                                                                                   gAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcattca                 <  2:318446/150‑1 (MQ=255)
                                                                                                                                   ataGCGGCGTCGCGGTACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACtcattca                 >  2:1156244/3‑150 (MQ=255)
                                                                                                                                       cggcgTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAg             <  1:801620/150‑1 (MQ=255)
                                                                                                                                         gcgTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGAc           <  1:1724392/150‑1 (MQ=255)
                                                                                                                                          cgTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGAcc          <  1:95203/150‑1 (MQ=255)
                                                                                                                                                  gacgacGCTATTTATCGTAATGTTTATCTTGCTGCTTTTTGTTGTTGATCATATCTCACAACATAGAGATCATCATTAACCGTACTTTAATGGGATAATGACTAAACACGCTCATATACCTCTTGAACTCATTCATAAGACCTCCTGACt  <  1:102447/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAAGAAGATAGAGCGAGCCGCCGTTCAGGTCAACTACTGACGAACAAATCTTTTGCGAAGGGGATTGCAAATCCGACAAAAAGTGGGGTTTTTCAGACCAAAACGCGTTATGCCTGCATGAAAGCAGGAGTGAAGCGGCGTACCGCGACGCCGCTATTTATCGGAAGGTTTATCTTGCTGCGGTTTGTTGTTGACCATATCGCACAACATAGAGAGCAGCATTAACCGTACTTTAAAGGGAGAATGACTAAACACGCGCATACACCTCTTGAACTCATTCATAAGACCTCCTGACT  >  NC_000913/852716‑853011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: